• 한국정밀공학회지
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• 제18권11호
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• pp.58-66
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• 2001

GPC has been reported as a useful self-tuning control algorithm for systems with unknown time-delay and parameters. GPC is easy to understand and implement, and thus has won popularity among many practicing engineers. Despite its success, GPC does not guarantee is nominal stability. So, in this paper, GPC is rederived in frequency domain instead of in the time domain to guarantee its nominal stability. Derivation of GPC in frequency domain involves spectral factorization and Diophantine equation. Frequency domain GPC control law is stable because the zeros of characteristic polynomial are strictly Schur. Recursive least square algorithm is used to identify unknown parameters. To see the effectiveness of the proposed controller, the controller is simulated for a numerical problem that changes in dead-time, in order and in parameters.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1992년도 한국자동제어학술회의논문집(국내학술편); KOEX, Seoul; 19-21 Oct. 1992
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• pp.188-193
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• 1992

The PID controllers which is widely used in the process industry are poorly damped when the dynamic process contains significant dead time or when there are random disturbances acting on the plant. GPC is known to be more superior than conventional self-tuning algorithm in overcoming above problem and prior choice of model order. In this paper, we propose the method which determine the parameter of PID controller from minimization of GPC criterion. The controller has emplicit scheme which is comprised of parameter estimation and PID control design. Simulation results show the performance of the proposed self-tuning PID controller.

• 미생물학회지
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• 제50권1호
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• pp.15-21
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• 2014

Pseudomonas sp. P7014 박테리아를 통한 큰느타리버섯 균사체의 생육촉진에 관한 연구가 수행되었다. 박테리아 배양액으로 부터 분리한 ethyl acetate 분획물(F5)에는 성장촉진물질(GPC)이 함유되어 있음을 확인하였다. TLC, HPLC, NMR 및 MS/MS분석법으로 확인한 바, indole acetic acid (IAA)로 확인되었다. 큰느타리버섯 균사체는 성장촉진물질(GPC)이 첨가된 PDA와 PDB 배지에서 빠른 성장을 보였다. 성장촉진물질(GPC)의 농도는 1.0 nM로 매우 낮았지만, 확인된 tryptophan은 IAA의 전구체로써 IAA가 아민화된 형태였다. 이들 결과는 박테리아에서 분비된 성장촉진물질(GPC)은 IAA이었고 큰느타리버섯 균사체의 생육촉진에 중요한 역할을 하는 것으로 확인되었다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1990년도 가을 학술발표회 논문집
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• pp.107-112
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• 1990

Chromatograms of asphalt cements were observed using high pressure - gel permeation chromatography (HP-GPC). The chromatogram characterzed profile of molecular size distribution of asphalt cements. Bituminous concretes using the asphalt cements were prepared in laboratory to measure mechanical properties, tensile strength (TS) and resilient modulus (MR), in dry and wet conditions. Three sources of AC-20 Asphalt cements and one aggregates were used for GPC test and strength test. Results of mechanical property tests and GPC tests were analyzed to evaluate correlation between those two results. Regression analysis showed strong correlation between a mechanical property and sliced percent areas of GPC profile. Mathematical models were developed for estimating mechanical properties of asphalt cement concrete based on HP-GPC data.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.1029-1031
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• 2015

Objective: Glypican-3 (GPC3) is reported to be an oncofetal protein that is a useful diagnostic immunomarker for hepatoblastoma. However, the results are not inclusive. This study systemically investigated the association between expression of GPC3 and pediatric hepatoblastoma. Methods: Clinical studies evaluating the association were identified using a predefined search strategy. GPC3 immunohistochemistry was applied in the pathological diagnosis of hepatoblastoma using the monoclonal antibodies with formalin-fixed and paraffin-embedded specimens. Positive predictive rates for the association between expression of GPC3 and pediatric hepatoblastoma were calculated. Results: Specimens from four clinical studies which including 134 patients with pediatric hepatoblastoma tested by GPC3 immunohistochemistry were considered eligible for inclusion. Systemic analysis showed that, in all patients, pooled positive predictive rate of the association between expression of GPC3 and pediatric hepatoblastoma was 95.5% (128/134). Conclusion: This systemic analysis suggests that the expression of glypican-3 is highly associated with the diagnosis of pediatric hepatoblastoma.

• Parasites, Hosts and Diseases
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• 제30권3호
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• pp.169-176
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• 1992

여섯 계통의 근교계 마우스떠에 상흡충의 기생기간과 경시적 산란력을 근거로 하여 숙주 기생충 관계의 계통별 차이를 구명하기 위해 마우스찰 20개의 간흡충 피낭유충을 경구적으로 감염시켰다. 감염 후 간흡충의 충란이 처음 검출될 때까지의 기간은 DDY 마우스에서 평균 21.2일로 가장 짧았고, GPC 21.48, BALB/c 및 DS 23.2 5, ICR 및 nude 23.4일의 순이 었다. 총산란기간 역시 DDY 마우스에서 164일로 가장 길었으며, GPC 132R, BALB/c 97일, nude 37일, DS 32일 및 ICR 28일의 순이었다. DDY 및 GPC 마우스에서는 간흡충의 산란수가 비교적 높고 안정적이었으나 나머지 4계통의 마우스에서는 관찰기간 동안 불규칙하였다. 이상의 성적으로 미루어 보아 마우스의 계통에 따라 간흡충의 숙주 기생충 관계의 차리를 확인하였고 DDY 마우스가 6계통중 가장 호적숙주임을 알았다.

• 대한토목학회논문집
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• 제12권4호
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• pp.49-57
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• 1992

본 연구(研究)는 노화(老化)에 의한 아스팔트시멘트의 물리적 성질변화를 크로마토그램에 근거(根據)하여 유성학적(流性學的)으로 고찰(考察)하였다. 3종류의 AC-20 아스팔트시멘트와 그것을 인공노화(人工老化)시킨 것 그리고 도로(道路)포장의 코어로부터 추출(抽出)해낸 자연노화(自然老化)된 아스팔트에 대하여 3가지 물리적 성질과, 고압(高壓) 겔 투과(透過) 크로마토그래프(HP-GPC)를 이용한 크로마토그램(분자입도분포(分子粒度分布))을 측정(測定)하였다. 이 물리적 성질과 HP-GPC 결과를 분석(分析)하여 노화전후(老化前後)의 크로마토그램과 물리적 성질의 상관관계(相關關係)를 고찰(考察)하였다. 물리적 성질로는 점도(粘度), 동점도(動粘度), 퇴입도(退入度)를 구하였으며, HP-GPC 시험에서는 THF가 순환용액으로 사용되고 RI 검출기가 사용되었다. 크로마토그램을 같은 시간간격(時間間隔)의 10구간으로 분할(分割)하여, 각 구간의 분할면적비(分割面積比)와 물리적 성질이 통계분석(統計分析)에 사용되었다. 이 연구결과(研究結果) 아스팔트시멘트의 물리적 성질은 크로마토그램 특성과 매우 밀접한 관계가 있음을 알 수 있었다. 또한 아스팔트의 노화는 유성학적으로 분자입도의 구성비가 변환되는 과정임을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7345-7349
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• 2013

Background: Because of the high prevalence of hepatocellular carcinoma (HCC) in Egypt, new markers with better diagnostic performance than alpha-feto protein (AFP) are needed to help in early diagnosis. The aim of this work was to compare the clinical utility of both serum and mRNA glypican3 (GPC3) as probable diagnostic markers for HCC among Egyptian patients. Materials and Methods: A total of 60 subjects, including 40 with HCC, 10 with cirrhosis and 10 normal controls were analyzed for serum GPC3 (sGPC3) by ELISA. GPC-3 mRNA from circulating peripheral blood mononuclear cells was amplified by RT-PCR. Both markers were compared to some prognostic factors of HCC, and sensitivity of both techniques was compared. Results: Serum glypican-3 and AFP were significantly higher in the HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity and specificity were (95% each) for sGlypican-3, (82.5% and 85%) for AFP, and (100% and 90%) for Glypican3 mRNA, and (80% and 95%) for double combination between sGPC3 and AFP respectively. Conclusion: Both serum GPC-3 and GPC-3mRNA are promising diagnostic markers for early detection of HCC in Egyptian patients. RT- PCR proved to be more sensitive (100%) than ELISA (95%) in detecting glypican3.

• 한국도로학회논문집
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• 제6권4호
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• pp.1-12
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• 2004

본 논문은 상온상태의 폐아스팔트 포장재료를 가열재활용하여 기층용뿐만 아니라 표층용으로 활용함에 있어 재생 아스팔트 바인더의 특성을 연구한 것이다. 4종류의 RAP을 가지고 RAP 자체의 기본 물성을 시험하였다. 배합설계는 표층에는 RAP을 10, 20%를 첨가하였고, 기층에는 10. 20, 30%를 첨가하였다. 재생혼합물의 신규바인더로는 AC 60-80을 선정하였다. 침입도, 점도, GPC, TFO. 저온균열 저항성을 평가하기 위한 BBR 실험을 수행하였다. 절대점도와 GPC에서의 대형입도분자(LMS)를 지수함수 회귀분석을 통해 $R^2$이 0.95 이상이었고 이것은 절대점도 추정에 GPC 결과가 상당히 정확함을 시사해주고 있다. RAP을 첨가한 재생 아스팔트 바인더의 PG 저온 등급은 일반 신규 바인더에 비해 한 단계 높은 등급을 나타내므로 저온균열에 대한 저항성은 약간 약한 것으로 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6341-6345
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• 2013

To clarify the role of stem cells in hepatocarcinogenesis, glypican-3 (GPC-3) and E-cadherin expression was investigated in embryonic cell lineages. Mouse embryonic stem cells (ESCs), hepatic progenitor cells (HPCs) and hepatocyte like cells (HCs), representing 0, 22 and 40 days of differentiation, respectively, were treated in vitro with diethylnitrosamine (DEN) at four doses (0, 1, 5 and 15 mM; G1, G2, G3 and G4, respectively) for 24 h and GPC-3 and E-cadherin expression was examined by relative quantitative real-time PCR and immunocytochemistry. GPC-3 mRNA expression was significantly different for G4 at day 0 (p<0.001) and for G4 at day 22 (p<0.01) compared with the control (G1). E-cadherin mRNA expression was significantly different for G3 and G4 at day 0 (p<0.05 and p<0.001, respectively), for G2 and G4 (p<0.05 and p<0.001, respectively) at day 22 and for G2 and G4 (p<0.01 and p<0.001, respectively) at day 40 compared with G1. Immunofluorescence staining for GPC-3 showed a membranous and/or granular expression in cytoplasm of ESCs and HPCs and granular and/or diffuse expression in cytoplasm of HCs, which were also stained by E-cadherin. DEN treatment increased GPC-3 expression in ESCs, HPCs and HCs, with increase of E-cadherin expression. Taken together, the expression of GPC-3 was altered by DEN treatment. However, its expression pattern was different at the stage of embryo stem cells and its derived hepatic lineage cells. This suggests that GPC-3 expression may be modulated in the progeny of stem cells during their differentiation toward hepatocytes, associated with E-cadherin expression.