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http://dx.doi.org/10.7845/kjm.2014.3076

Identification of Auxin from Pseudomonas sp. P7014 for the Rapid Growth of Pleurotus eryngii Mycelium  

Kang, Young Min (Herbal Medicine Resources Group, Herbal Medicine Research Division, Korean Institute of Oriental Medicine (KIOM))
Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
Publication Information
Korean Journal of Microbiology / v.50, no.1, 2014 , pp. 15-21 More about this Journal
Abstract
The promoting effect of Pseudomonas sp. P7014 on the mycelia growth of Pleurotus eryngii was investigated. An ethyl acetate fraction (F5) from the culture supernatant of the bacteria was confirmed to contain the growth promoting compound (GPC). The GPC was identified to be indole acetic acid (IAA) by TLC, HPLC, MS/MS, and NMR analyses. P. eryngii mycelia grew rapidly both on PDA and in PDB after the treatment of GPC. The promoting concentration of GPC was as low as 1.0 nM. Tryptophan, the aminated form of IAA, was confirmed to be the precursor of IAA. These results suggested that bacterial secreted compound was IAA and plays an important role in promoting growth of mushroom mycelia.
Keywords
Pleurotus eryngii; Pseudomonas sp. P7014; bioassay; growth promotion; indole acetic acid;
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1 Wang, Y., Han, K.S., Wang, X.Y., Koh, Y.J., and Hur, J.S. 2009. Effect of ribitol and plant hormones on aposymbiotical growth of the lichen-forming fungi of Ramalina farinacea and Ramalina fastigiata. Mycobiology 37, 28-30.   DOI   ScienceOn
2 Wang, X.Y., Li, W.X., Luo, H., Kim, J.A., Jeon, H.S., Koh, Y.J., and Hur, J.S. 2010. Plant hormones promote growth in lichen-forming fungi. Mycobiology 38, 176-179.   DOI   ScienceOn
3 Wood, D.A. and Hammond, J.B.W. 1977. Ethylene production by axenic fruiting cultures of Agaricus bisporus. Appl. Environ. Microbiol. 34, 228-229.
4 Xie, H., Pasternak, J.J., and Glick, B.R. 1996. Isolation and characterization of mutants of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 that overproduce indoleacetic acid. Curr. Microbiol. 32, 67-71.   DOI   ScienceOn
5 Rahman, A., Amakawa, T., Goto, N., and Tsurumi, S. 2001. Auxin is a positive regulator for ethylene-mediated response in the growth of Arabidopsis roots. Plant Cell Physiol. 42, 301-307.   DOI   ScienceOn
6 Omer, Z.S., Tombolini, R., Broberg, A., and Gerhardson, B. 2004. Indole-3-acetic acid production by pink-pigmented facultative methylotrophic bacteria. Plant Growth Regul. 43, 93-96.   DOI   ScienceOn
7 Peck, S.C. and Kende, H. 1995. Sequential induction of the ethylene biosynthetic enzymes by indole-3-acetic acid in etiolated peas. Plant Mol. Biol. 28, 293-301.   DOI   ScienceOn
8 Pilet, P.E. and Saugy, M. 1987. Effect on root growth of endogenous and applied IAA and ABA. Plant Physiol. 83, 33-38.   DOI   ScienceOn
9 Silva, E.M., Machuca, A., and Milagres, A.M.F. 2005. Effect of cereal brans on Lentinula edodes growth and enzyme activities during cultivation on forestry waste. Lett. Appl. Microbiol. 40, 283-288.   DOI   ScienceOn
10 Steenhoudt, O. and Vanderleyden, J. 2000. Azospirillum, a freeliving nitrogen fixing bacterium closely associated with grasses:genetic, biochemical and ecological aspects. FEMS Microbiol. Rev. 24, 487-506.   DOI   ScienceOn
11 Tien, T.M., Gaskins, M.H., and Hubbell, D.H. 1979. Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl. Environ. Microbiol. 37, 1016-1024.
12 Uhart, M., Piscera, J.M., and Alberto, E. 2008. Utilization of new naturally occurring strains and supplementation to improve the biological efficiency of the edible mushroom Agrocybe cylindracea. J. Ind. Microbiol. Biotechnol. DOI 10.1007/s10295-008-0321-1.   DOI
13 Grewal, S.I. and Rainey, P.B. 1991. Phenotypic variation of Pseudomonas putida and P. tolaasii affects the chemotactic response to Agaricus bisporus mycelial exudate. J. Gen. Microbiol. 137, 2761-2768.   DOI
14 Kim, M.K., Math, R.K., Cho, K.M., Shin, K.J., Kim, J.O., Ryu, J.S., Lee, Y.H., and Yun, H.D. 2008. Effect of Pseudomonas sp. P7014 on the growth of edible mushroom Pleurotus eryngii in bottle culture for commercial production. Bioresour. Technol. 99, 3306-3308.   DOI
15 Glick, B.R., Karaturovic, D.M., and Newell, P.C. 1995. A novel procedure for rapid isolation of plant growth promoting pseudomonads. Can. J. Microbiol. 41, 533-536.   DOI   ScienceOn
16 Glick, B.R. and Patten, C.L. 2002. Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl. Environ. Microbiol. 68, 3795-3801.   DOI   ScienceOn
17 Loper, J.E. and Schroth, M.N. 1986. Influence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet. J. Phytopathol. 76, 386-389.   DOI
18 Mark, S., Valentina, K., Yulia, M., Alexander, G., Andrey, P., Andrey, S., Nikolay, P., and Nina, D. 2004. Immunomodulating and anti-tumor action of extracts of several mushrooms. J. Biotechnol. 113, 77-83.   DOI   ScienceOn
19 Meuwley, P. and Pilet, P.E. 1991. Local treatment with indole-3-acetic acid induces differential growth responses in Zea mays L. roots. Planta 185, 58-64.
20 Mukhopadhyay, R., Chatterjee, S, Chatterjee, B.P., and Guha, A.K. 2005. Enhancement of biomass production of edible mushroom Pleurotus sajor-caju grown in whey by plant growth hormones. Process Biochem. 40, 1241-1244.   DOI   ScienceOn
21 Barbieri, R. and Galli, E. 1993. Effect on wheat root development of inoculation with an Azospirillum brasilense mutant with altered indole-3-acetic acid production. Res. Microbiol. 144, 69-75.   DOI   ScienceOn
22 Obodai, M., Cleland-Okine, J., and Vowotor, K.A. 2003. Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. J. Ind. Microbiol. Biotechnol. 30, 146-149.   DOI
23 Okon, Y. and Vanderleyden, J. 1997. Root-associated Azospirillum species can stimulate plants. ASM News 63, 366-370.
24 Biswas, J.C., Ladha, J.K., and Dazzo, F.B. 2000. Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Soil Sci. Soc. Am. J. 64, 1644-1650.   DOI
25 Beyerler, M., Michaux, P., Keel, C., and Haas, D. 1997. Effect of enhanced production of indole-3-acetic acid by the biological control agent Pseudomonas fluorescens CHA0 on plant growth, pp. 310-312. In Ogoshi, A., Kobayashi, K., Homma, Y., Kodama, F., Kondo, N., and Akino, S. (ed.), Plant growthpromoting rhizobacteria: present status and future prospects. OECD, Paris, France.
26 Cho, Y.S., Kim, J.S., Crowley, D.E., and Cho, B.G. 2003. Growth promotion of the edible fungus Pleurotus ostreatus by fluorescent pseudomonads. FEMS Microbiol. Lett. 218, 271-276.   DOI   ScienceOn
27 Eckardt, N.A. 2001. New insights into auxin biosynthesis. Plant Cell. 13, 1-3.   DOI
28 Alvarez, R., Nissen, S.J., and Sutter, E.G. 1989. Relationship between indole-3-acetic acid levels in apple (Malus pumila Mill.) rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid. Plant Physiol. 89, 439-443.   DOI   ScienceOn
29 Bano, Z. and Rajarathnnam, S. 1987. Pleurotus mushroom, part IA. Morphology, life cycle, taxonomy, breeding and cultivation. Crit. Rev. Food Sci. Nutr. 26, 157-233.   DOI   ScienceOn
30 Arshad, M. and Frankenberger, W.T.Jr. 1998. Plant growth regulating substances in the rhizosphere: microbial production and functions. Advan. Agron. 62, 146-151.
31 Frankenberger, W.T. and Arshad, M.Jr. 1995. Phytohormones in soil: Microbial production and function, p. 503. Marcel Dekker Inc., NY, USA.
32 Sawar, M. and Kremmer, R.J. 1995. Enhanced suppression of plant growth through production of L-tryptophan compounds by deleterious rhizobacteria. Plant Soil 172, 261-269.   DOI   ScienceOn
33 Barazani, O. and Friedman, J. 1999. Is IAA the major root growth factor secreted from plant-growth-mediating bacteria? J. Chem. Ecol. 25, 2397-2406.   DOI   ScienceOn