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Chemical Properties of Soils in the Vicinity of Yoch의on Industrial Complex (여천공단 주변지역 토양의 화학적 성질)

  • 문형태;표재훈;김준호
    • The Korean Journal of Ecology
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    • v.21 no.1
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    • pp.1-6
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    • 1998
  • Effects of air pollutants on the chemical properties of soil in Yoch'on inductrial complex were investigated. Soil pH showed severe acidity with average value of 4.3$\pm$0.13. Soil pH decreaed along the increase in altitute of Mt. Jeseok. Soil organic matter and total N was 19.2$\pm$3.6% and 4.7$\pm$0.25mg/g, respectively, which were higher than those of unpolluted area, Kongju. Available P was 41.2$\pm$3.7 $\mu$g/g, which was 4 times greater than that of unpolluted area, Kongju. Average content of total-S was 283.5$\pm$14.1 $\mu$g/g, which was 10 times greater than that of unpolluted area. Content of available Al was o.43$\pm$0.03 mg/g, which was 2 times greater than that of unpolluted area. Available Al increased as the soil pH decrease. Contents of exchangeable K, Ca and Mg were significantly lower than those in unpolluted area, because of severe leaching in acid soil.

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Growth Characteristics of Rhoodotorula sp. Y-55 on Ethanol, Acetic acid, and Acetaldehyde Substrates (Ethanol, Acetic acid, Acetaldehyde 기질에서의 Rhoodotorula sp. Y-55의 증식 특징)

  • Yeehn Yeeh;Sang Hae Kim;Woo Hong Joo;Hong Ki Jun;Oh Change Kwon
    • KSBB Journal
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    • v.11 no.3
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    • pp.367-373
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    • 1996
  • The growth characteristics of Rhodotorula sp. Y-55 were examined on minimal medium containing ethanol, acetic acid or acetadehyde as a sole carbon source by batch culture. The increased concentration of substrate reduced overall growth yield and prolonged lag time. The specific growth rate of the yeast was changed, depending upon the initial concentrations of ethanol and acetaldehyde during the exponential period, but was constant on acetic acid without regard to the initial substrate concentrations, giving a value of 0.l07h-1. The highest ${\mu}$ value was obtained on ethanol and acetadehyde substrates and the respective values were 0.270 at 20g/L and 0.041h-1 at 0.2g/L. The maximum overall growth yields were appeared to be 32.6% for ethanol of 10g/L, 25.6% for acetic acid of 20g/L, and 45% for acetaldehyde of 0.2g/L. The respective cellular contents of crude protein and nucleic acids were determined to be 41.5 and 4.9wt% on ethanol and 40.2 and 4.7wt% at the concentration revealing maximal growth yield.

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Anti-tumor Effect of Carrot(Docus carota L.) Extracts in the Human Lung Cancer Cell Line NCI-H1299 (인체 페암세포주 NCI-H1299에 대한 당근 추출물의 항암효과)

  • 노숙령;김도희
    • Journal of the East Asian Society of Dietary Life
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    • v.12 no.4
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    • pp.289-298
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    • 2002
  • This study was designed to investigate the anti-tumor effects of fresh carrot juice, methanol-extracts, and $\beta$-carotene on the human lung cancer cell line NCI-H1299. The anti-tumor effect was evaluated by the MTT assay in vitro. The anti-tumor effect of fresh carrot juice against NCI-H1299 lasted up to 96 hours after exposure; the viability rate of lung cancer cells decreased below 50% after 48 hours, and further after 72 hours. The strongest propagation inhibition effect of fresh carrot juice was shown at the concentration of 2000 $\mu\textrm{g}$/$m\ell$ after 72 hours and the viability rates was 45.98% even at the concentration of 25 $\mu\textrm{g}$/$m\ell$. The value of $IC_{50}$/ was 23.1$\mu\textrm{g}$/$m\ell$ when the elapsed time was 72 hours. The viability rate of methanol-extract was 52.4% under the concentration of 2000 $\mu\textrm{g}$/$m\ell$ and the elapsed time of 72 hours. Under the concentration of 1000 $\mu\textrm{g}$/$m\ell$ and the elapsed time of 48 hours, $\beta$ -carotene decreased the viability rate to 29.99%. The $IC_{50}$/ value of $\beta$-carotene was 691.2$\mu\textrm{g}$/$m\ell$ after 72 hours. According to the above results, the anti-tumor effect arose in NCI-H1299 when the concentration of the fresh carrot juice or the $\beta$-carotene was more than 25 $\mu\textrm{g}$/$m\ell$ or 1000 $\mu\textrm{g}$/$m\ell$, respectively. On the other hand, the methanol-extracts showed a weak anti-tumor effect even at a concentration as high as 2000 $\mu\textrm{g}$/$m\ell$.

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Analysis on the Frumkin Adsorption Isotherm of the Over-Potentially Deposited Hydrogen (OPD H) at the Polycrystalline Ni | Alkaline Aqueous Electrolyte Interface Using the Phase-Shift Method

  • Chun Jang H.;Jeon Sang K.
    • Journal of the Korean Electrochemical Society
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    • v.4 no.4
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    • pp.146-151
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    • 2001
  • The Frumkin adsorption isotherm of the over-potentially deposited hydrogen (OPD H) for the cathodic $H_2$ evolution reaction (HER) at the poly-Ni|0.05M KOH aqueous electrolyte interface has been studied using the phase-shift method. The behavior of the phase shift $(0^{\circ}\leq{\phi}\leq90^{\circ})$ for the optimum intermediate frequency corresponds well to that of the fractional surface coverage $(1\geq{\theta}\geq0)$ at the interface. The phase-shift method, i.e., the Phase-shift profile $(-{\phi}\;vs.\;E)$ for the optimum intermediate frequency, can be used as a new method to estimate the Frumkin adsorption isotherm $(\theta\;vs.\;E)$ of the OPD H for the cathodic HER at the interface. At the poly-Ni|0.05M KOH aqueous electrolyte interface, the rate (r) of change of the standard free energy of the OPD H with $\theta$, the interaction parameter (g) for the Frumkin adsorption isotherm, the equilibrium constant (K) for the OPD H with $\theta$, and the standard free energy $({\Delta}G_{\theta})$ of the OPD H with ${\theta}$ are $24.8kJ mol^{-1},\;10,\;5.9\times10^{-6}{\leq}K{\leq}0.13,\;and\;5.1\leq{\Delta}G_{\theta}\leq29.8kJ\;mol^{-1}$. The electrode kinetic parameters $(r,\;g,\;K,\;{\Delta}G_{\theta})$ depend strongly on ${\theta} (0{\leq}{\theta}{\leq}1)$.

Pharmacokinetics of oxytetracycline in olive flounder (Paralichthys olivaceus) by intramuscular injection (Oxytetracycline을 근육 주사한 넙치 (Paralichthys olivaceus)의 약물동태학적 특성)

  • Jung, Sung-Hee;Choi, Dong-Lim;Kim, Jin-Woo;Jo, Mi-Ra;Seo, Jung-Soo;Ji, Bo-Young
    • Journal of fish pathology
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    • v.22 no.1
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    • pp.91-95
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    • 2009
  • The pharmacokinetic properties of oxytetracycline (OTC) were studied after intramuscular injection to cultured olive flounder, Paralichthys olivaceus. Plasma concentrations of OTC were determined after dosage of 12.5, 25 and 50 ㎎/㎏ body weight in olive flounder (average 600 g, $23{\pm}1{^{\circ}C}$). Plasma samples were taken at 3, 5, 10, 15, 24, 32, 48, 72, 120, 168, 240 and 360 h post-dose. With 25 and 50 ㎎/㎏, the peak plasma concentrations of OTC, which attained at 5 h post-dose, were 0.99 and 1.49 $\mu{g}/m\ell$, respectively. However, the peak plasma concentration of 12.5 ㎎/㎏ was 0.35 $\mu{g}/m\ell$ after 10 h post-dose. Plasma concentrations of OTC were not measurable at 360 h post-dose in all doses. The kinetic profile of absorption, distribution and elimination of OTC in plasma were analyzed fitting to a 1-compartment model by Win-Nonlin program. The following parameters were calculated for 12.5, 25 and 50 ㎎/㎏ body weight, respectively: AUC (the area under the concentration-time curve)?D���D24.98, 44.67 and 50.45 $\mu{g}$ $h/m\ell$ $T_{1/2}$ (half-life) ?D���D0.42, 0.59 and 0.41 h; $T_{max}$ (time for maximum concentration)?D���D8.46, 6.34 and 2.66 h; $C_{max}$ (maximum concentration)?D���D0.30, 0.63 and 1.13 $\mu{g}/m\ell$.

Physicochemical components of Astragalus membranaceus fermented with mushroom mycelia (담자균 균사체가 배양된 황기의 이화학적 성분분석)

  • Jang, Yeon-Jeong;Lee, Yun-Hye;Lee, Chan-Jung;Kim, Jae-Hyeon;Kim, Eun-Ju;Ji, Soo-Jeong;Park, Shin-Young
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.49-56
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    • 2016
  • This study analyzed the physicochemical characteristics of Astragalus membranaceus (AM) fermented with seven different mushroom mycelia. Physicochemical characteristics, such as contents of moisture, pH, total reducing sugars, free sugar, and isoflavonoid, were investigated. The moisture content was increased in most of the samples. The pH values of AM fermented with Phellinus linteus and Flammulina velutipes were increased, while the pH of other samples were similar to that of non-fermented AM. The reducing sugar content was in the range of 211.69~391.74 mg/100 g. The extraction yield using water was higher than that when extracted with 80% ethanol. The free sugar content was increased through fermentation with mushroom mycelia. However, the glucose contents of the 80% ethanol and water extracts were decreased. Finally, the calycosin and formononetin contents in 80% ethanol and water extracts of AM fermented with Phellinus linteus were 2,549.24 mg/g, and 827.66 mg/g for calycosin, and 1,366.69 mg/g and 221.28 mg/g for formononetin, respectively. These results suggest that fermentation with mushroom mycelia could be used to increase the bioactivity of AM. The mycelium-fermented AM might be a valuable source of functional material and edible resource for industry.

Optimal Cultur Conditions for the Production of Insecticidal Toxin by Xenorhabdus nematophilus Isolated from Steinernema carpocapsae (Steinernema carpocapsae로부터 분리된 Xenorhabdus nematophilus에 의한 살충물질 생산을 위한 최적 배양조건)

  • 유연수;박선호
    • KSBB Journal
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    • v.15 no.1
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    • pp.100-105
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    • 2000
  • Optimal medium composition, culture conditions, characteristics of phase variation and activity of insecticidal toxin by Xenorhabdus nematophilus isolated and identified from Korean entomopathogenic nematode Steinernema carpocapsae were examined. Optimal medium composition of this strain was 50-70 g/L yeast extract, 3 g/L $K_{2}HPO_{4}$, 1g/L $NH_{4}H_{2}PO_{4}$, 2g/L ${MgSO}_4$$\cdot$${7H}_{2}O$, 10g/L NaCl and, these, yeast extract was found as a limiting nutrient for cell growth. When Monod equation was applied, maxmum specific growth rate and Monod constant were estimated as 0.13 $hr^{-1}$ and 20g/L, respectively. The pH of culture medium increased up to 8.5-9.5 regardless of initial pH 6-7 as the cells continued to grow. The specific growth rate in a 7 L fermentor was 0.18 $hr^{-1}$, which was enhancement 1.4 fold compared to a flask culture. In case of phase variation, phase I fraction was maintained above 90% at the stationary phase for both flask and fermentor cultures. According to oral toxicity test of Gallena mellonella by Xenorhabdus nematophilus, the addition of cell pellets into feed inhibited normal growth of insect larvae and killed completely then after 20 days cultivation. When culture supernatant of this strain was injected into hemolymph of insect larva, the toxicity was strongest at 24hr cultivation in the early exponential phase and gradually decreased as the culture time proceeded.

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Enlargement of Anti-corrosion of Zinc Plating by the Trivalent Chromium Sulfate Conversion Coating (3가 크롬황산염의 크로메이트에 의한 아연도금내식성 증대)

  • Lee, Chul-Tae
    • Applied Chemistry for Engineering
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    • v.18 no.3
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    • pp.296-302
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    • 2007
  • A trivalent chromate conversion coating solution which is composed with $KCr_2(SO_4)$ as main component was investigated to test a feasibility of use as an alternative six-valent chromate conversion coating for improvement of anti-corrosion of zinc plating. The proposed trivalent convesion coating was consisted of $KCr(SO_4)$ 35~45 g/L as trivalent chromium source, $NaH_2PO_4$ 20~30 g/L as phosphate, $CoSO_4$, 10~20 g/L, $ZnSO_4$ 10~20 g/L as metallic sulfates. This trivalent chromate films which are coated by this chromate conversion coating solution under pH 2.0~2.2, immersion time of 20~25 s at room temperature are able to achieve over 120 h in neutral salt spray test to 5% white rust.

A Seasonal Variation of Acidic Gases and Fine Particle Species in Chongju Area (청주지역 산성 가스상물질과 미세입자의 계절 변동 특성)

  • 강병욱;이학성;김희강
    • Journal of Korean Society for Atmospheric Environment
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    • v.13 no.5
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    • pp.333-343
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    • 1997
  • The cyclone/annular denuder system/filter pack sampling system (ADS) was used to collect the acidic air pollutants in Chongju city. The data set was collected on fifty -eight different days with 24 hour sampling period from October 27, 1995 through August 25, 1996. The chemical species measured were HN $O_3$, HN $O_2$, S $O_2$ and N $H_3$ in the gas phase, and PM2.5( $d_{p}$ <2.5 ${\mu}{\textrm}{m}$), S $O_4$$^{2-}$, N $O_3$$^{[-10]}$ and N $H_4$$^{+}$ in the Particulate Phase. Mean concentrations measured for this study were: 0.45 $\mu\textrm{g}$/㎥ for HN $O_3$, 3.39 $\mu\textrm{g}$/㎥ for HN $O_2$, 26.4 $\mu\textrm{g}$/㎥ for S $O_2$, 3.83$\mu\textrm{g}$/㎥ for N $H_3$, 44.2 $\mu\textrm{g}$/㎥ for P $M_{2.5}$, 8.22 $\mu\textrm{g}$/㎥ for S $O_4$$^{2-}$, 3.63 $\mu\textrm{g}$/㎥ for N $O_3$$^{[-10]}$ , and 2.84 $\mu\textrm{g}$/㎥ for N $H_4$$^{- }$. HN $O_3$ and N $H_3$ were higher during the summer. However, HN $O_2$ and S $O_2$ were higher during the fall and winter. P $M_{2.5}$ , S $O_4$/ sup 2-/ and N $H_4$$^{+}$ were not showed seasonal variations, but N $O_3$$^{[-10]}$ was higher in the winter.ter.r.

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