• Plant Resources
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• v.6 no.2
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• pp.129-133
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• 2003

For mass multiplication of an important medicinal plant Coleus forskohlii, a procedure for the high frequency regeneration of Coleus forskohlii has been developed using leaf explants via callus culture. Callus formation occurred in MS medium supplemented with 1-2 mg/L each of NAA and BAP. A large number of shoots were formed on MS + 1 mg/L BAP from 50-60 days old greenish calli. Rooting of healthy shoots occurred on 0.1-0.4 mg/L NAA. The protocol described could be useful in future for genetic manipulation of this plant species.

• Dementia and Neurocognitive Disorders
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• v.17 no.4
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• pp.131-136
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• 2018

Alzheimer's disease (AD) related genes have been elucidated by advanced genetic techniques. Familial autosomal dominant AD genes founded by linkage analyses are APP, PSEN1, PSEN2, ABCA7, and SORL1. Genome-wide association studies have found risk genes such as ABCA7, BIN1, CASS4, CD33, CD2AP, CELF1, CLU, CR1, DSG2, EPHA1, FERMT2, HLA-DRB5-HLA-DRB1, INPP5D, MEF2C, MS4A6A/MS4A4E, NME8, PICALM, PTK2B, SLC24A4, SORL1, and ZCWPW1. ABCA7, SORL1, TREM2, and APOE are proved to have high odds ratio (>2) in risk of AD using next generation sequencing studies. Thanks to the promising genetic techniques such as CRISPR-CAS9 and single-cell RNA sequencing opened a new era in genetics. CRISPR-CAS9 can directly link genetic knowledge to future treatment. Single-cell RNA sequencing are providing useful information on cell biology and pathogenesis of diverse diseases.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2B
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• pp.107-120
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• 2010

The effect of potential future climate change on the inflow of agricultural reservoir and its impact to downstream streamflow by reservoir operation for paddy irrigation water was assessed using the SLURP (semi-distributed land use-based runoff process), a physically based hydrological model. The fundamental input data (elevation, meteorological data, land use, soil, vegetation) was collected to calibrate and validate of the SLURP model for a 366.5 $km^2$ watershed including two agricultural reservoirs (Geumgwang and Gosam) located in Anseongcheon watershed. Then, the CCCma CGCM2 data by SRES (special report on emissions scenarios) A2 and B2 scenarios of the IPCC (intergovernmental panel on climate change) was used to assess the future potential climate change. The future weather data for the year, m ms, m5ms and 2amms was downscaled by Change Factor method through bias-correction using 3m years (1977-2006) weather data of 3 meteorological stations of the watershed. In addition, the future land uses were predicted by modified CA (cellular automata)-Markov technique using the time series land use data fromFactosat images. Also the future vegetation cover information was predicted and considered by the linear regression between monthly NDVI (normalized difference vegetation index) from NOAA AVHRR images and monthly mean temperature using eight years (1998-2006) data.

• Journal of Plant Biotechnology
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• v.46 no.1
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• pp.32-36
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• 2019

Calla lilies (Zantedeschia spp.) are monocotyledonous ornamental plants which belongs to the Araceae family. After the release of elite calla cultivar, an efficient propagation system is needed for commercial use. Despite the use of conventional propagation methods such as splitting of tubers and rhizomes of calla, rapid and efficient propagation system should be developed. In order to achieve this goal, stem segments contained apical meristems derived from calla lily cultivar (cv. Gag-si) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxin. This was aimed at inducing embryogenic calluses, shoots and multiple shoots. As a result, about 25% of induction rates of yellow embryogenic calluses were observed with MS medium containing both $0.5mg{\cdot}L^{-1}\;NAA$ and $1.5mg{\cdot}L^{-1}\;BA$ as growth regulators. In the experiments involving the regeneration from embryogenic calluses through shoot formation, MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ showed the highest rates at approximately 85 ~ 90% with regard to the formation of shoots in calla. Moreover, multiple shoots needed for rapid propagation were generated when explants were cultured on MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ with 40% of formation rate. In this study, the combination of auxin and cytokinin showed positive effects on both the induction of embryogenic calluses, the formation of shoots as well as multiple shoots in calla. The regeneration system described here can contribute to the development of breeding programs of calla in the future.

• Journal of Convergence for Information Technology
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• v.11 no.2
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• pp.194-200
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• 2021

The aim of this study was proceed for 6weeks to determine the effect of middle-aged women's upper lips ms by SUKI programs. A total of 20 subjects were tested, and the SUKI arbitration programs of Pattern T1, Pattern T2, Pattern T3, Pattern T4 were adopted three times a week in six weeks. The results were as this. It showed a significant difference in the EG, although some of the intra-group comparisons of the upper lip ms. Therefore, SUKI programs have been shown to help improve the elasticity of the upper lip ms(p<.05). In this study, the SUKI program was used based on the fact that although limited, the elasticity of the upper lip ms, located in the facial part of a middle-aged woman, causes various wrinkles around her mouth, making them lose confidence in their appearance. This also suggests that normal facial ms can affect the appearance of the female middle-aged woman, and it was intended to suggest some ways to manage the appearance by proper management of facial muscle according to the life cycle of the female middle-aged woman. In conclusion, we hope that in the future, various experiments will be used as new research data on how to prevent women's facial skin beauty and wrinkles and help improve elasticity of facial muscles around the mouth.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.39 no.6
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• pp.687-695
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• 2002

Media-specific FEC techniques, suggested to confront with VoIP speech packet loss, improve speech quality at the expense of generating additional one-frame delay. In this paper, we suggest new media-specific FEC, i.e, LSF FEC technique which is able to improve speech quality with much shortened additional delay. In the proposed technique, the LSF parameters of the future frame are utilized to recover a lost packet. To evaluate performance of the proposed technique, we use ITU-T G.723.1 and G.729 Codec and apply Gilbert packet loss model and estimate MOS per every packet loss rate using PESQ speech quality estimation algorithm. The proposed technique has effect of shortening delay over from 6.5ms to 27ms compared with existing media-specific FEC techniques. Simulation results for comparison of reconstructed speech quality show this novel technique improves the MOS over 0.1 in practical lossy environment of 5 % packet loss rate.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.15 no.1
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• pp.61-70
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• 2005

This study investigated the spinal loads(L5/S1 disc compression and shear forces) predicted from four biomechanical models: one EMG model and three optimization models. Three objective functions used in the optimization models were to miminize 1) the cubed muscle forces : MF3, 2) the cubed muscle stress : MS3, 3) maximum muscle intensity : MI. Twelve healthy male subjects participated in the isometric voluntary exertion tests to six directions : flexion/extension, left/right lateral bending, clockwise/ counterclockwise twist. EMG signals were measured from ten trunk muscles and spinal loads were assessed at 10, 20, 30, 40, 50, 60, 70, 80, 90%MVE(maximum voluntary exertion) in each direction. Three optimization models predicted lower L5/S1 disc compression forces than the EMG model, on average, by 31%(MF3), 27%(MS3), 8%(MI). Especially, in twist and extension, the differences were relatively large. Anterior-posterior shear forces predicted from optimization models were lower, on average, by 27%(MF3), 21%(MS3), 9%(MI) than by the EMG model, especially in flexion(MF3 : 45%, MS3 : 40%, MI : 35%). Lateral shear forces were predicted far less than anterior-posterior shear forces(total average = 124 N), and the optimization models predicted larger values than the EMG model on average. These results indicated that the optimization models could underestimate compression forces during twisting and extension, and anterior-posterior shear forces during flexion. Thus, future research should address the antagonistic coactivation, one major reason of the difference between optimization models and the EMG model, in the optimization models.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.297-302
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• 2019

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus 'Nanpa' using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus 'Nanpa' can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus 'Nanpa' in the future.

• ETRI Journal
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• v.42 no.5
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• pp.721-733
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• 2020

Recently, there is an increasing demand for ultra-low-latency (ULL) services such as factory automation, autonomous driving, and telesurgery that must meet an end-to-end latency of less than 10 ms. Fifth-generation (5G) New Radio guarantees 0.5 ms one-way latency, so the feasibility of ULL services is higher than in previous mobile communications. However, this feasibility ensures performance at the radio access network level and requires an innovative 5G network architecture for end-to-end ULL across the entire 5G system. Hence, we survey in detailed two the 3rd Generation Partnership Party (3GPP) standardization activities to ensure low latency at network level. 3GPP standardizes mobile edge computing (MEC), a low-latency solution at the edge network, in Release 15/16 and is standardizing time-sensitive communication in Release 16/17 for interworking 5G systems and IEEE 802.1 time-sensitive networking (TSN), a next-generation industry technology for ensuring low/deterministic latency. We developed a 5G system based on 3GPP Release 15 to support MEC with a potential sub-10 ms end-to-end latency in the edge network. In the near future, to provide ULL services in the external network of a 5G system, we suggest a 5G-IEEE TSN interworking system based on 3GPP Release 16/17 that meets an end-to-end latency of 2 ms.

• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.205-211
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• 2013

The swine is one of the most widespread mammalian throughout the whole world. Presently, many studies concerning microsatellites in swine, especially domestic pigs, have been carried out in order to investigate general diversity patterns among either populations or breeds. Until now, a lot of time and effort spend into a single PCR method. But simple and more rapid multiplex PCR methods have been developed. The purpose of this study is to develop a robust set of microsatellites markers (MS marker) for traceability and individual identification. Using multiplex-PCR method with 23 MS marker divided 2 set, various alleles occurring to 5 swine breed (Berkshire, Landrace, Yorkshire, Duroc and Korea native pig) used markers to determine allele frequency and heterozygosity. MS marker found 4 alleles at SW403, S0227, SWR414, SW1041 and SW1377. The most were found 10 alleles at SW1920. Heterozygosity represented the lowest value of 0.102 at SWR414 and highest value of 0.861 at SW1920. So, it was recognized appropriate allele frequency for individual identification in swine. Using multiplex-PCR method, MS markers used to determine individual identification biomarker and breed-specific marker for faster, more accurate and lower analysis cost. Based on this result, a scientific basis was established to the existing pedigree data by applying genetics additionally. Swine traceability is expected to be very useful system and be conducted nationwide in future.