In vitro propagation of Coleus forskohlii, an important medicinal plant

  • Yang, Deok-hun (Department of Oriental medicinal materials & processing Kyung Hee university) ;
  • Jeon, Manju-Meluttu-George (Department of Oriental medicinal materials & processing Kyung hee University) ;
  • Jong-Seong (Graduate school of Biotechnology & Plant Metabolism Research Center Kyung hee University)
  • Published : 2003.08.01

Abstract

For mass multiplication of an important medicinal plant Coleus forskohlii, a procedure for the high frequency regeneration of Coleus forskohlii has been developed using leaf explants via callus culture. Callus formation occurred in MS medium supplemented with 1-2 mg/L each of NAA and BAP. A large number of shoots were formed on MS + 1 mg/L BAP from 50-60 days old greenish calli. Rooting of healthy shoots occurred on 0.1-0.4 mg/L NAA. The protocol described could be useful in future for genetic manipulation of this plant species.

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