• Culinary science and hospitality research
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• v.18 no.3
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• pp.1-17
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• 2012

This study was planned for an empirical review of foodservice operation in expressway rest areas for a menu strategy targeting staff and customers. The results were as follows. Main foodservice category included 10-30 items of Korean dishes. The needs for having branded foodservice menu in rest areas were high in fusion/fast foods, snack/ready-made and beverages. Employment of professional cooks in rest areas was low (10-30%), and the most important factor was 'taste' when cooking Korean foods. Foreigners preferred fusion/fast foods and snack/ready-made foods to Korean food, beverages and Japanese/Chinese foods, and most of the foreigners ordered food with 'no question'(55.5%) or 'some questions'(34.8%). Rest area staff prepared some photos/pictures of foods (80-100%) for menu comprehension, but the accuracy of menu transcription was very low. Customers used a rest area once or twice, stayed for 10-20 min(43.6%)/20-30 min(38.6%), visited by car(77.7%) to relax and use toilets(61.6%) on average. Favorite food and snacks were Korean(32.7%) and ready made/snacks(33.0%), and they spent 5,000-10,000 won(51.3%), 3,000-5,000 won(25.8%) on them. Customer satisfaction with rest areas was 'average' regardless of gender, age, education levels and jobs. With this analysis, we should prepare an efficient menu strategy for customer satisfaction and plans for analyzing problems along with alternatives.

• The Plant Pathology Journal
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• v.26 no.1
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• pp.25-31
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• 2010

Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus-P biotype (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector, sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein was over-expressed as a fusion protein in Escherichia coli. SDS-PAGE gel revealed that CP expressed as a ~40 kDa protein. The recombinant coat protein (rCP) fused with 6x His-tag was purified from E.coli using Ni-NTA resin. The antigenicity of the fusion protein was determined by western blot analysis using antibodies raised against purified PRSV. The purified rCP was used as an antigen to produce high titer PRSV specific polyclonal antiserum. The resulting antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) assay and compared its sensitivity levels with ELISA based assays for detection of PRSV isolates. IC-RT-PCR was shown to be the most sensitive test followed by dot-blot immunobinding assay (DBIA) and plate trapped ELISA.

• The Plant Pathology Journal
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• v.32 no.3
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• pp.173-181
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• 2016

Gene disruption by homologous recombination is widely used to investigate and analyze the function of genes in Fusarium fujikuroi, a fungus that causes bakanae disease and root rot symptoms in rice. To generate gene deletion constructs, the use of conventional cloning methods, which rely on restriction enzymes and ligases, has had limited success due to a lack of unique restriction enzyme sites. Although strategies that avoid the use of restriction enzymes have been employed to overcome this issue, these methods require complicated PCR steps or are frequently inefficient. Here, we introduce a cloning system that utilizes multi-fragment assembly by In-Fusion to generate a gene disruption construct. This method utilizes DNA fragment fusion and requires only one PCR step and one reaction for construction. Using this strategy, a gene disruption construct for Fusarium cyclin C1 (FCC1), which is associated with fumonisin B1 bio-synthesis, was successfully created and used for fungal transformation. In vivo and in vitro experiments using confirmed fcc1 mutants suggest that fumonisin production is closely related to disease symptoms exhibited by F. fujikuroi strain B14. Taken together, this multi-fragment assembly method represents a simpler and a more convenient process for targeted gene disruption in fungi.

• Journal of IKEEE
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• v.22 no.3
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• pp.558-564
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• 2018

In distributed estimation where each node can collect only partial information on the parameter of interest without communication between nodes and quantize it before transmission to a fusion node which conducts estimation of the parameter, we consider a novel quantization technique employed at local nodes. It should be noted that the performance can be greatly improved if each node can transmit its measurement to one designated node (namely, head node) which can quantize its estimate using the total rate available in the system. For this case, the best strategy at the head node would be simply to partition the parameter space using the generalized Lloyd algorithm, producing the global codewords, one of which is closest to the estimate is transmitted to a fusion node. In this paper, we propose an iterative design algorithm that seeks to efficiently assign the codewords into each of quantization partitions at nodes so as to achieve the performance close to that of the system with the head node. We show through extensive experiments that the proposed algorithm offers a performance improvement in rate-distortion perspective as compared with previous novel techniques.

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.3
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• pp.163-168
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• 2021

Unlike in the past, profit-making companies use design thinking to solve their customers' essential needs rather than just solving problems in a conventional way. Design syncing is a creative strategy to solve problems by using designers' senses and methods in the process of design. Design thinking is a new way of thinking for designers to design their own ways and solve problems. In other words, design thinking can solve complex problems in a new and creative way. In order to realize the necessary functions, an aggregation of relevant elements in accordance with the new law can create an innovative design. Additionally, if a convergence system is applied that is organized and regularly functional to accomplish the functions of different kinds, design-thinking outcomes can make the necessary functions more specific. Through our research, we would like to explore the differential features of design and discuss the direction of design for consumer needs through a case analysis of design thinking with creative convergence system. We would like to propose competitive design product development and creative design thinking through case analysis such as products and systems with design thinking applied. We hope that this research will help businesses and individuals who make design thinking a problem.

• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.718-724
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• 2017

The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application. Therefore, an acidic tail of synuclein (ATS) derived from the C-terminal acidic tail of human alpha-synuclein protein was fused to the C-terminus of scFv57R in order to help it resist adverse stress and improve the stability and half-life. The tail showed no apparent effect on the preparation procedure and affinity of the protein, nor did it change the neutralizing potency in vitro. In the ELISA test of molecular stability, the ATS fusion form of the protein, scFv57R-ATS, showed an increase in thermal stability and longer half-life in serum than scFv57R. The protection against fatal rabies virus challenge improved after fusing the tail to the scFv, which may be attributed to the improved stability. Thus, the ATS fusion approach presented here is easily implemented and can be used as a new strategy to improve the stability and half-life of engineered antibody proteins for practical applications.

• Journal of Korean Library and Information Science Society
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• v.46 no.1
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• pp.321-344
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• 2015

This paper presents a fusion approach to sentiment detection that combines multiple sources of evidence to retrieve blogs that contain opinions on a specific topic. Our approach to finding opinionated blogs on topic consists of first applying traditional information retrieval methods to retrieve blogs on a given topic and then boosting the ranks of opinionated blogs based on the opinion scores computed by multiple sentiment detection methods. Our sentiment detection strategy, whose central idea is to rely on a variety of complementary evidences rather than trying to optimize the utilization of a single source of evidence, includes High Frequency module, which identifies opinions based on the frequency of opinion terms (i.e., terms that occur frequently in opinionated documents), Low Frequency module, which makes use of uncommon/rare terms (e.g., "sooo good") that express strong sentiments, IU Module, which leverages n-grams with IU (I and you) anchor terms (e.g., I believe, You will love), Wilson's lexicon module, which uses a collection-independent opinion lexicon constructed from Wilson's subjectivity terms, and Opinion Acronym module, which utilizes a small set of opinion acronyms (e.g., imho). The results of our study show that combining multiple sources of opinion evidence is an effective method for improving opinion detection performance.

• KIPS Transactions on Software and Data Engineering
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• v.12 no.12
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• pp.505-518
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• 2023

3D point cloud semantic segmentation is a computer vision task that involves dividing the point cloud into different objects and regions by predicting the class label of each point. Existing 3D semantic segmentation models have some limitations in performing sufficient fusion of multi-modal features while ensuring both characteristics of 2D visual features extracted from RGB images and 3D geometric features extracted from point cloud. Therefore, in this paper, we propose MMCA-Net, a novel 3D semantic segmentation model using 2D-3D multi-modal features. The proposed model effectively fuses two heterogeneous 2D visual features and 3D geometric features by using an intermediate fusion strategy and a multi-modal cross attention-based fusion operation. Also, the proposed model extracts context-rich 3D geometric features from input point cloud consisting of irregularly distributed points by adopting PTv2 as 3D geometric encoder. In this paper, we conducted both quantitative and qualitative experiments with the benchmark dataset, ScanNetv2 in order to analyze the performance of the proposed model. In terms of the metric mIoU, the proposed model showed a 9.2% performance improvement over the PTv2 model using only 3D geometric features, and a 12.12% performance improvement over the MVPNet model using 2D-3D multi-modal features. As a result, we proved the effectiveness and usefulness of the proposed model.

• Journal of Korean Society for Quality Management
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• v.46 no.3
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• pp.483-496
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• 2018

Purpose: This paper proposes a quality strategy based on the evolution of technology in the age of the 4th Industrial Revolution. Methods: We examine the theory of past quality activities and the changes in quality paradigm, and analyze key words for the technologies and key issues of the 4th Industrial Revolution. Based on existing quality management, we find a quality strategy that should be pursued during the 4th Industrial Revolution. Results: Quality has been recognized as an essential component of corporate competitiveness. The paradigm of quality has also changed with the pass of time and industry development. From this viewpoint, the following eight quality strategies are proposed for the development of the technology of the 4th Industrial Revolution period, such as Market-to-customer fusion quality, symbiotic quality, big data quality, technical accuracy and zero-defect quality, facility predictability quality, software quality, process flexibility quality, and information protection stability and security quality. Conclusion: Quality for customer satisfaction is still important nowadays. However, in the 4th Industrial Revolution era, where various business models and methods of manufacturing are expected, the big data utilization, software quality, and the reliability and security of information protection to support it are important.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.316-319
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• 2011

During the past several decades, the development of the maritime policy have been focused on the level of regulation based on the lessons from the individual accidents. Recently, the development of IMO e-navigation strategy has been discussed actively and has been focusing on the new frame and architecture based on the user requirements and definition of function and services for user requirements, which might produce the user-friendly policy, infrastructure and user service. For the new maritime ICT policy getting fusion of ICT and innovation of ship/ocean industry, the overarching methodology, as IMO e-navigation strategy, should be applied to primarily setup the architecture from the user requirements to technology. In this paper, we analyze the IMO e-navigation strategy procedure and provide the consideration and lessons for the strategic methodology to develop a new maritime ICT policy.