• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.1
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• pp.1-10
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• 2014

At present, there are few research papers on skin penetration of cosmeceutical ingredients. What is worse is that in vivo studies are hard to find. In this study, we measured skin epidermal penetration of cosmeceutical ingredients using in vivo Raman spectroscopy and compared with the results obtained from experiments using in vitro franz cell. Results showed that ascorbyl-2-glucoside, retinol, retinyl palmitate, and kojic acid were good for penetration ratio in measurement in vitro and retinol, vitamin C, and arbutin were good in measurement in vivo. Among them, retinol was best in skin penetration in vivo experiment using Raman spectroscopy and ascorbyl-2-glucoside was best in skin penetration in vitro experiment using Franz cell system. It is estimated that the differences were originated from the experimental procedures of two different methods; in vivo Raman experiment can be sensitive to the effect of epidermis and dermis as characteristics of matter by estimating the stratum corneum and in vitro measurement is evaluation of material to penetrate skin of hairless mouse. However, most penetration barrier is the stratum corneum, thus it is important to examine movement of material in the stratum corneum. We expect that these results provided useful information for many cosmetic related research.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.111-117
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• 2011

Objectives : The present study was designed to investigate effects of mixed extract from korean medicinal herbs (MIX) on oxidation/reduction reaction-related and aging-related enzyme in vitro. Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase inhibition assay. Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. The MIX showed 97% inhibition of collagenase activity, and 64% inhibition of elastase activity at 1 mg/ml concentration of MIX, next only to positive control, which indicate good efficacy for anti-wrinkle ingredient. Also it's treatment showed 34% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of MIX. Antioxidant activities were determined by DPPH radical scavenging, xanthine oxidase (XO) inhibiting activity and SOD-like activity. Also these scavenging, XO-inhibiting and SOD-like activities were measured in 91%, 80%, and 63% inhibition, respectively, at a treated dose of 1 mg/ml, compare to control. Conclusions : These results suggest that possibility of mixed korean medicinal herbs as a functional ingredient for anti-wrinkle and whitening, anti-oxidation and anti-aging cosmetic formula.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.49-49
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• 2021

The roots of reed (Phragmites australis) were used in traditional medicine to treat respiratory problems, including symptoms such as high fever and cough. In this study, we identified the active ingredient from 70% EtOH reed root extract, and evaluated the whitening, wrinkle improvement and moisturizing effects. The content of p-coumaric acid, the active ingredient of the roots of P. australis, was slightly lower in 70% EtOH extract than in 100% EtOH extract. However, 70% EtOH reed root extract showed similar or higher effect in reducing power, DPPH, hydrogen peroxide scavenging, and nitric oxide scavenging activity compared to 100% EtOH extract. Moreover, 70% EtOH reed root extract markedly inhibited melanogenesis in B16F10 cells treated with α-melanocyte-stimulating hormone. 70% EtOH reed root extract significantly inhibited the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced elastase activity in HDF human dermal fibroblasts. In addition, 70% EtOH reed root extract ameliorated hyaluronic acid synthase-2 (HAS-2) expression induced by ultraviolet B (UVB) stimulation in HaCaT keratinocytes. The results of this study suggest that 70% EtOH reed root extract has potential as a functional cosmetic material related to whitening, wrinkle improvement, and moisturizing.

• Journal of the Korean Data and Information Science Society
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• v.25 no.6
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• pp.1273-1281
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• 2014

As a variety of industrial technology advances with various materials developed, cosmetics industry in general is witnessing many kinds of new cosmetic products. At the same time, Cosmetics Act becomes effective, and cosmetics ingredient labeling is implemented. Concerns with life pattern such as life management, biorhythm, right posture, and stress symptoms are on the increase in order to seek high-quality life as well. These life patterns are closely connected not only with life quality and health but with skin management, and thus this paper conducts a survey on the connection between functional cosmetics awareness and life pattern. Results show that out of 16 questions concerning cosmetics awareness, responses to question number 3, 5, 6 and 11 have relevant differences among colleges. Furthermore, the results of cross analysis with life pattern show that there are relevant differences between the year of responses and stress symptoms and between the year of responses and right posture. Lastly, it is shown that answers to questions regarding gender and biorhythm, gender and right posture, and gender and stress symptoms are relevantly different. Further research is needed to reveal the differences between college students and ordinary people at large.

• Korean Journal of Agricultural Science
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• v.45 no.3
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• pp.499-508
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• 2018

To use mountain ginseng adventitious roots (MGAR), which is superior in functionality compared to ginseng, and to mass produce raw materials as a functional cosmetic material, the antioxidant and cosmeceutical activities of the extracts of MGAR at different ethanol concentrations (0, 20, 40, 60 and 80%) were compared and analyzed. At first, different ethanol concentrations were compared to determine the best solvent for the extraction of phenolic compounds from MGAR. An 80% ethanol extract with 10.07 mg of gallic acid equivalents/g sample and 0.94 mg of quercetin equivalents/g sample was the best solvent in the extraction of phenolic compounds. However, the 60% ethanol extract had the highest antioxidant activity, evident by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, Trolox equivalent antioxidant capacity (35.01 mM Trolox/g sample) and ferric reducing antioxidant power (460.94 mM $FeSO_4/g$ sample). However, the inhibitory activities of the 80% ethanol extract from the MGAR against tyrosinase and elastase were higher than those of the other extracts. The results of this study show that the extract of MGAR can be used as an easily accessible source of natural antioxidant and as a possible cosmetic ingredient in the cosmeceutical industry. However, the components responsible for the antioxidant and cosmeceutical activities of the MGAR extracts are currently unclear. Therefore, it is suggested that further studies should be carried out to isolate and identify the antioxidant and cosmeceutical components in MGAR.

• Journal of Applied Biological Chemistry
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• v.53 no.3
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• pp.162-169
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• 2010

The solvent extracts of Prunus persica Flos were investigated for the activities of anti-oxidant and anti-inflammation to apply as a functional ingredient for cosmetic products. The electron donating ability of both ethanol (PPE) or acetone (PPA) extracts of P. persica Flos was above 90.0% at the concentration of 500ppm. The superoxide dismutase (SOD)-like activity of P. persica Flos extracts (PPE, PPA) were approximately 40.0% at 1,000 ppm. The xanthine oxidase inhibitory effect of P. persica Flos extracts (PPE, PPA) was approximately 30.0% at 1,000 ppm and equivalent to that of ascorbic acid. Hyaluronidase inhibition activity related to the anti-inflammation effect was 35.0% with the treatment of P. persica Flos extracts (PPW, PPE, PPA) at 1,000 ppm, respectively. In the experiment of anti-inflammation effect, P. persica Flos extracts (PPW, PPE, PPA) inhibited the generation of nitric oxide. In the antimicrobial activity test against the human skin-resident microflora such as Staphylococcus epidermidis and Propionibacterium acnes, a clear zone was identified from 4mg/disc in P. persica Flos (PPE) extract.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.9-14
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• 2018

In order to find new functional materials for the cosmetics application, we investigated the anti-oxidant and anti-melanogenic properties of Gentiana scabra extracts (GSE), which were prepared by the various extraction methods. Results showed that GSE had high DPPH radical scavenging activity in a dose-dependent manner. Also, GSE inhibited the prodution of melanin in B16F10 melanoma cell as well as tyrosinase activity. We also found that GSE inhibited mRNA expression of tyrosinase-related protein (TRP)-1 and 2. In conclusion we suggest that GSE is applicable to cosmetics as a potential ingredient for their anti-oxidant and whitening effects.

• The Korean Journal of Mycology
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• v.47 no.2
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• pp.153-163
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• 2019

This study was carried out to investigate the feasibility of Salix gracilistyla production for cosmetic use through mycelial fermentation. The efficacy of this method was confirmed by fermentation using the mycelia of Ganoderma lucidum (a representative medicinal mushroom). Total polyphenol and flavonoid content and DPPH radical scavenging activity of S. gracilistyla extract (SGE) were found to be higher than those of G. lucidum fermented S. gracilistyla extract (GLSGE). GLSGE had relatively lower collagenase activity than SGE. However, GLSGE increased HDFn cell viability more potently than SGE, and increased the biosynthesis of type I procollagen. Thus, GLSGE could be used as an anti-aging cosmetic active ingredient. These results indicate that extract fermentation using G. lucidum mycelia can effectively enhance some beneficial effects of functional materials.

• Journal of the Korean Applied Science and Technology
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• v.39 no.2
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• pp.303-313
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• 2022

In this study, the extracted oils and commercial oils (Clarisse) were analyzed after GC-MSD fragrance component analysis for each natural essential oil obtained through steam distillation extraction (cypress, seokchangpo, lavender) of a total of three plants grown and grown in Jangheung area. Easy, cinnamon, frankincense) was combined to confirm cytotoxicity, antioxidant, anti-inflammatory, and whitening effects, confirming its potential as a cosmetic material. As a result of the analysis of fragrance components, sabinene was identified as the main component of cypress oil, asarone in seokchangpo oil, and L-Linalool in lavender oil. At a concentration of 100 𝜇L/mL with no cytotoxicity of 6 essential combination oils, NO production was inhibited by 27.76%, DPPH radical scavenging activity was 99.69%, ABTS radical scavenging activity was 94.66%, and tyrosinase inhibitory activity was 55.9%. Possibility of being useful as a raw material for functional cosmetics was presented.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.4
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• pp.375-383
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• 2023

Malic acid-catalyzed transformation has been developed to produce ginsenoside Rg3 which is increasingly in demand as a functional ingredient. The optimization of the conversion of red ginseng saponin (RGS) to ginsenoside Rg3 by acid catalyzed transformation was carried out using Box-Behnken design (BBD) based on Response Surface Analysis (RSM). The main independent variables were malic acid concentration, temperature, and reaction time. Conversion of ginsenoside Rg3 was performed according to BBD model and optimization conditions were analyzed. The concentration of the converted ginsenoside Rg3 ranged from 1.548 mg/L to 4.558 mg/L, and the highest production was obtained under the condition of reacting 1% malic acid, 50 ℃ and 9h. Consequently, The independent variables affecting the production of ginsenoside Rg3 were identified in the following order: malic acid concentration, reaction time and temperature. In addition, it was confirmed that the interaction between malic acid concentration and reaction time had a greater influence than the temperature.