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Application of next generation sequencing (NGS) system for whole-genome sequencing of porcine reproductive and respiratory syndrome virus (PRRSV) (돼지생식기호흡기증후군바이러스(PRRSV)의 전장 유전체 염기서열(whole-genome sequencing) 분석을 위한 차세대 염기서열 분석법의 활용)

  • Moon, Sung-Hyun;Khatun, Amina;Kim, Won-Il;Hossain, Md Mukter;Oh, Yeonsu;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.39 no.1
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    • pp.41-49
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    • 2016
  • In the present study, fast and robust methods for the next generation sequencing (NGS) were developed for analysis of PRRSV full genome sequences, which is a positive sensed RNA virus with a high degree of genetic variability among isolates. Two strains of PRRSVs (VR2332 and VR2332-R) which have been maintained in our laboratory were used to validate our methods and to compare with the sequence registered in GenBank (GenBank accession no. EF536003). The results suggested that both of strains had 100% coverage with the reference; the VR2332 had the coverage depth from minimum 3 to maximum 23,012, for the VR2332-R from minimum 3 to maximum 41,348, and 22,712 as an average depth. Genomic data produced from the massive sequencing capacities of the NGS have enabled the study of PRRSV at an unprecedented rate and details. Unlike conventional sequence methods which require the knowledge of conserved regions, the NGS allows de novo assembly of the full viral genomes. Therefore, our results suggested that these methods using the NGS massively facilitate the generation of more full genome PRRSV sequences locally as well as nationally in regard of saving time and cost.

Optimal Distribution of Viscoelastic Material for Transient Vibration Suppression of a Flexible Beam (유연보의 과도 진동 감쇠를 위한 점탄성 재료의 최적 분포)

  • Kim, Tae-Woo;Kim, Ji-Hwan
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2002.11b
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    • pp.605-610
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    • 2002
  • Eigenvalues are taken as performance criteria for structural damping design using viscoelastic material. Given material properties, optimal distribution of damping material is sought based on eigenvalue sensitivity. For eigenanalysis of frequency dependent viscoelastic material treated structures, Golla-Mushes-McTavish (GHM) model is used and some dominant modes are chosen for consideration. To avoid the intensity of computation caused by increased problem size, an alternative approximate method is proposed which uses elastic modes and can be applied under small damping assumption. A cantilever beam treated with unconstrained viscoelastic layer is tested and optimal distribution of thickness of the layer is illustrated. Partial coverage configurations are compared with the one-sided full coverage case.

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Cognitive Radio Anti-Jamming Scheme for Security Provisioning IoT Communications

  • Kim, Sungwook
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.10
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    • pp.4177-4190
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    • 2015
  • Current research on Internet of Things (IoT) has primarily addressed the means to enhancing smart resource allocation, automatic network operation, and secure service provisioning. In particular, providing satisfactory security service in IoT systems is indispensable to its mission critical applications. However, limited resources prevent full security coverage at all times. Therefore, these limited resources must be deployed intelligently by considering differences in priorities of targets that require security coverage. In this study, we have developed a new application of Cognitive Radio (CR) technology for IoT systems and provide an appropriate security solution that will enable IoT to be more affordable and applicable than it is currently. To resolve the security-related resource allocation problem, game theory is a suitable and effective tool. Based on the Blotto game model, we propose a new strategic power allocation scheme to ensure secure CR communications. A simulation shows that our proposed scheme can effectively respond to current system conditions and perform more effectively than other existing schemes in dynamically changeable IoT environments.

Optimal Power Allocation and Outage Analysis for Cognitive MIMO Full Duplex Relay Network Based on Orthogonal Space-Time Block Codes

  • Liu, Jia;Kang, GuiXia;Zhu, Ying
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.3
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    • pp.924-944
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    • 2014
  • This paper investigates the power allocation and outage performance of MIMO full-duplex relaying (MFDR), based on orthogonal space-time block codes (OSTBC), in cognitive radio systems. OSTBC transmission is used as a simple means to achieve multi-antenna diversity gain. Cognitive MFDR systems not only have the advantage of increasing spectral efficiency through spectrum sharing, but they can also extend coverage through the use of relays. In cognitive MFDR systems, the primary user experiences interference from the secondary source and relay simultaneously, owing to full duplexing. It is therefore necessary to optimize the transmission powers at the secondary source and relay. In this paper, we propose an optimal power allocation (OPA) scheme based on minimizing the outage probability in cognitive MFDR systems. We also analyse the outage probability of the secondary user in noise-limited and interference-limited environments in Nakagami-m fading channels. Simulation results show that the proposed schemes achieve performance improvements in terms of reducing outage probability.

A Partial Scan Design by Unifying Structural Analysis and Testabilities (구조분석과 테스트 가능도의 통합에 의한 부분스캔 설계)

  • Park, Jong-Uk;Sin, Sang-Hun;Park, Seong-Ju
    • Journal of KIISE:Computer Systems and Theory
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    • v.26 no.9
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    • pp.1177-1184
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    • 1999
  • 본 논문에서는 스캔플립프롭 선택 시간이 짧고 높은 고장 검출률(fault coverage)을 얻을 수 있는 새로운 부분스캔 설계 기술을 제안한다. 순차회로에서 테스트패턴 생성을 용이하게 하기 위하여 완전스캔 및 부분스캔 설계 기술이 널리 이용되고 있다. 스캔 설계로 인한 추가영역을 최소화 하고 최대의 고장 검출률을 목표로 하는 부분스캔 기술은 크게 구조분석과 테스트 가능도(testability)에 의한 설계 기술로 나누어 볼 수 있다. 구조분석에 의한 부분스캔은 짧은 시간에 스캔플립프롭을 선택할 수 있지만 고장 검출률은 낮다. 반면 테스트 가능도에 의한 부분스캔은 구조분석에 의한 부분스캔보다 스캔플립프롭의 선택 시간이 많이 걸리는 단점이 있지만 높은 고장 검출률을 나타낸다. 본 논문에서는 구조분석에 의한 부분스캔과 테스트 가능도에 의한 부분스캔 설계 기술의 장단점을 비교.분석하여 통합함으로써 스캔플립프롭 선택 시간을 단축하고 고장 검출률을 높일 수 있는 새로운 부분스캔 설계 기술을 제안한다. 실험결과 대부분의 ISCAS89 벤치마크 회로에서 스캔플립프롭 선택 시간은 현격히 감소하였고 비교적 높은 고장 검출률을 나타내었다.Abstract This paper provides a new partial scan design technique which not only reduces the time for selecting scan flip-flops but also improves fault coverage. To simplify the problem of the test pattern generation in the sequential circuits, full scan and partial scan design techniques have been widely adopted. The partial scan techniques which aim at minimizing the area overhead while maximizing the fault coverage, can be classified into the techniques based on structural analysis and testabilities. In case of the partial scan by structural analysis, it does not take much time to select scan flip-flops, but fault coverage is low. On the other hand, although the partial scan by testabilities generally results in high fault coverage, it requires more time to select scan flip-flops than the former method. In this paper, we analyzed and unified the strengths of the techniques by structural analysis and by testabilities. The new partial scan design technique not only reduces the time for selecting scan flip-flops but also improves fault coverage. Test results demonstrate the remarkable reduction of the time to select the scan flip-flops and high fault coverage in most ISCAS89 benchmark circuits.

Factors of Influencing the Benefit Amount according to the National Health Insurance Coverage in Pit and Fissure Sealants (치면열구전색 급여화에 따른 수혜량에 영향을 미치는 요인)

  • Ahn, Eun-Suk;Hwang, Ji-Min
    • Journal of dental hygiene science
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    • v.15 no.6
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    • pp.768-774
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    • 2015
  • The purpose of this study was to grasp the benefits from pit and fissure sealants and to analyze the factors of influencing the benefit amount by using the raw data of the Korea National Health and Nutrition Examination Survey for 2007~2013 targeting the subjects aged from 6 years in full to 18 years. The analysis was used STATA 11.0. As a result of the analysis, the following conclusions were obtained. It rose up to 1.24 pieces after carrying out the national health insurance coverage from averagely 0.93 piece until the year in 2007~2009 (up to November) when is before enforcing the national health insurance coverage in the pit and fissure sealants. The benefits from pit and fissure sealants depending on general characteristics were surveyed to be higher in the higher income level, in the more subscription to health insurance and private insurance, and in the more toothbrushing frequency per day. The factors that have influence upon the benefits from pit and fissure sealants were shown to include whether or not to have the national health insurance coverage in the pit and fissure sealants, income level, health insurance type and private insurance subscription appearance, and one-day toothbrushing frequency. Synthesizing the results, a rise in the benefits from pit and fissure sealants is shown in 2010 based on December 2009 when the national health insurance coverage in the pit and fissure sealants was implemented, but is showing the tendency of declining again from 2011. To increase the benefits from pit and fissure sealants, it is thought that the schemes will need to be discussed such as reinforcing publicity on the national health insurance coverage in the pit and fissure sealants, expanding a support for low-income bracket, and differentiating the outpatient cost sharing according to socio-economic level.

Underwriting Method of Worksite Marketing Product (Worksite Marketing 상품과 언더라이팅 기법)

  • Kim, Cheong-Nyun;Chung, Sung-Wan
    • The Journal of the Korean life insurance medical association
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    • v.24
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    • pp.97-117
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    • 2005
  • Internally Korean insurance market is that whole life products' growth are becoming slowdown that's why new insurance products have appeared on the market in consideration of consumer's needs recently. Externally domestic insurance market competitions has drifted from insurance industry to whole financial industry since bankasurance started. Life insurance companies should open up a new market to survive from severe competitions. Worksite marketing can be an alternative. An insurer make arrangements with an employer about an insurance terms which an insurer offers in Worksite marketing. Then eligible individuals enroll in the plans at their own discretion and pay 100 percent of the premium for coverage through payroll deductions. An employer doesn't need to pay extra money for additional benefit but can raise employee's loyalty and satisfaction of company through worksite marketing. An employee can be covered at discounted premium rate and less strict underwriting guidelines to an insurer compared to individual insurance. In developed countries specially U.S insurance market, Worksite marketing is getting very popular and growing rapidly due to the advantages. Worksite marketing has both individual insurance characters and group insurance characters. Individual insurance characters are that employees enroll in the plans at their own discretion and pay 100 percent of the premium for coverage. Group insurance characters are that actively at work and participation etc. An insurer have to reflect these two characters on Worksite marketing when an insurance company work out a plan for developing products and underwriting guidelines. When an insurer devise worksite products, one should consider participation level which means percentage of eligible employees participating. Participation is related to anti-selection. As we know underwriting is essential for every kind of insurance, especially underwriting plays major role in worksite marketing. We can see that in the below. Firstly, it has a function in calculation of premium rate. When calculate premium rate for worksite products underwriters have to estimate expected participation level and risk factors. So underwriters and acturies keep in close contact with each other. Secondly, underwriting methods are important. When an insurer underwrite worksite products, there are three kinds of underwriting methods. These are Simplified issue underwriting, Full underwriting and Guaranteed issue underwriting. Simplified issue underwriting typically requires no medical examination, but usually requires supplying satisfactory answers to one or several health and/or lifestyle questions. Full underwriting requires a complete medical history questionnaire that may further require an exam. Guaranteed issue underwriting means that coverage is issued without the employee having to provide evidence of insurability. When insurer set the GI limit are usually based on the type of industry, number of eligible employees, the average amount of coverage and participation level. In addition to insurer should have a clear definition of eligible employee on the insurance provision and application form. It will minimize possibility of trouble claims and anti-selection. An insurer also establish preexisting condition exclusion and special guidelines for late entrants. When an insurer introduce Worksite marketing to Korean insurance market, an insurer has to examine market research to analyze potential market and strategy of sales most of all. Also an insurer should review real situation of the U.S, England and Japanese market etc. There are a lot of new technologies about worksite marketing process that an insurer should learn. When an insurer consider many things which we explained it can be a real alternative.

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Restorative management using hybrid ceramic of a patient with severe tooth erosion from swimming: a clinical report

  • Peampring, Chaimongkon
    • The Journal of Advanced Prosthodontics
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    • v.6 no.5
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    • pp.423-426
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    • 2014
  • This clinical report presents the clinical appearance and treatment approach in a case of excessive anterior teeth erosion resulted from swimming in a poorly-chlorinated swimming pool. Clinical findings revealed tooth sensitivity, severe enamel erosion resembling veneer preparations, and the presence of anterior open bite. A novel hybrid ceramic (Vita Enamic) was chosen for fabricating full-coverage crowns for this patient. After 6-months follow-up, the tooth sensitivity disappeared and the patient was satisfied with esthetic outcome. The hybrid ceramic restorations can be recommended with no complications.

Analysis of 'Sleep Disease' Medical Service Delivery system Through In-Depth Interview

  • Yu, Tae Gyu
    • International Journal of Advanced Culture Technology
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    • v.8 no.2
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    • pp.1-5
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    • 2020
  • As the world gradually advances to an aging society, the quality of human life is valued. Among them, 'quality of sleep' is very closely related to quality of life. Recently, Korea expanded health insurance coverage for "sleep disorders". Particularly, as the number of sleep multiple tests and prescriptions for sleep aids has increased rapidly, much attention has been focused on the related medical service environment. Therefore, this study looked at an in-depth interview of 11 hospitals to see what treatment delivery system is being established when the government applies health insurance for 'sleep disorders'. In conclusion, the organizations with the most average number of sleep polyp tests per day were found to have more sleep polyp labs (hardware) and more full-time specialists. Also, the polysomnography lab (hardware) and the specialist's full-time status (software) did not necessarily result in a "positive pressure regulator prescription" that can solve "sleep apnea" caused by "sleep ailments". Rather, it was found that the number of days of sleep multiple laboratories (hardware), the number of full-time specialists (software) or the specialty majors (software) had a greater impact. In particular, the higher the specialist's full-time personnel (software) index (=6.000), the higher the sleep-inducing agent prescription rate(=1.000), and the lower the specialist's full-time personnel (software) index (=1.000), the higher the sleep-inducer's prescription rate(= 0.010) Was low. In addition, even if the professional full-time personnel(software) index was the same (=1.000), the hospital type was lower as it was closer to the public hospital(=0.067) and higher at the specialized hospital (= 0.933). In the case of university hospitals, when the full-time specialists (software) are in the same condition (= 1.000), the frequency of use of the sleep laboratory (=1.000) and the sleep test rate (= 1.000) were all the same.

Scan Selection Algorithms for No Holding Partial Scan Test Method (무고정 부분 스캔 테스트 방법을 위한 스캔 선택 알고리즘)

  • 이동호
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.35C no.12
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    • pp.49-58
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    • 1998
  • In this paper, we report new algorithms to select scan flip-flops for the no holding partial scan test method. The no holding partial scan test method is identical to the full scan test method except that some flip-flops are left unscanned. This test method does not hold scanned or unscanned flip-flops while shifting in test vectors, or applying them, or shifting out test results. The proposed algorithm allows a large number of flip-flops to be left unscanned while maintaining almost the complete full scan fault coverage.

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