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http://dx.doi.org/10.7853/kjvs.2016.39.1.41

Application of next generation sequencing (NGS) system for whole-genome sequencing of porcine reproductive and respiratory syndrome virus (PRRSV)  

Moon, Sung-Hyun (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Khatun, Amina (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kim, Won-Il (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Hossain, Md Mukter (Department of Medicine, Faculty of Veterinary & Animal Science, Sylhet Agricultural University)
Oh, Yeonsu (Department of Veterinary Pathology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Cho, Ho-Seong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.39, no.1, 2016 , pp. 41-49 More about this Journal
Abstract
In the present study, fast and robust methods for the next generation sequencing (NGS) were developed for analysis of PRRSV full genome sequences, which is a positive sensed RNA virus with a high degree of genetic variability among isolates. Two strains of PRRSVs (VR2332 and VR2332-R) which have been maintained in our laboratory were used to validate our methods and to compare with the sequence registered in GenBank (GenBank accession no. EF536003). The results suggested that both of strains had 100% coverage with the reference; the VR2332 had the coverage depth from minimum 3 to maximum 23,012, for the VR2332-R from minimum 3 to maximum 41,348, and 22,712 as an average depth. Genomic data produced from the massive sequencing capacities of the NGS have enabled the study of PRRSV at an unprecedented rate and details. Unlike conventional sequence methods which require the knowledge of conserved regions, the NGS allows de novo assembly of the full viral genomes. Therefore, our results suggested that these methods using the NGS massively facilitate the generation of more full genome PRRSV sequences locally as well as nationally in regard of saving time and cost.
Keywords
Next Generation Sequencing; NGS; PRRSV; Whole-genome sequencing;
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