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Control Effect of Sodium Dichloroisocyanurate for Pear Scab (Venturia nashicola) on Niitaka Pear during Flowering Period (신고배 개화기 NaDCC 처리에 의한 검은별무늬병의 방제)

  • Nam, Ki-Woong;Han, Mi-Kyong;Yoon, Deok-Hoon
    • Korean Journal of Organic Agriculture
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    • v.22 no.2
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    • pp.347-357
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    • 2014
  • This study was conducted to evaluate the control effect of sodium dichloroisocyanurate (NaDCC) for Venturia nashicola on Niitaka pear during flowering period. As a nontoxic disinfectant, sodium dichloroisocyanurate is widely used in the field of hygiene and disease prevention, medical treatment, aquiculture as well as plant protection. NaDCC was sprayed on the pear tree inoculated with conidia ($4.5{\times}10^5spores/mL$) of Venturia nashicola and as a result the incidence of pear scab was 23.8% in 750mg/L and 26.2% in 1,000mg/L compare to the 51.6% incidence in untreated tree. No damage in the pollen of pear flower was detected with NaDCC treatments in the full bloom period for six pear cultivar including Wonwhang. A NaDCC single treatment in the early bloom of Niitaka pear showed more than 98% of fertilization rate. Furthermore, there was no incidence of Venturia nashicola on Nitaka pear trees treated with NaDCC 4 times during their growth period.

Pathogenicity and Host Range of a Potential Mycoherbicide, Isolate BWC98-105, Causing White Root Rot on Trifoliorum repens

  • Hong, Yeon-Kyu;Cho, Jae-Min;Lee, Bong-Choon;Song, Seok-Bo;Park, Sung-Tae
    • The Plant Pathology Journal
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    • v.20 no.1
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    • pp.58-62
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    • 2004
  • White root rot of wild white clover (Trifoliorum repens) caused by isolate BWC98-105 has been first reported in Korea. Typical symptoms on root include water-soaked and dark-brown rot, resulting in complete blight of the whole plant. The fungus grew well at $20-28^{\circ}C$ and produced abundant sclerotia at 10-15 days after full mycelial growth on potato dextrose agar. Sclerotia were brown to dark-brown in color and 1-3 mm in length. When white clover plants were inoculated with mycelial suspension ($10^5$ cfu/ml) of isolate BWC98-105, the plant shoots were killed within 4-6 days and the roots were completely blighted. Sclerotia were also formed on the surface of the root covered with whitish mycelia within 10-15 days in the field. All nine isolates developed high incidences of white root rot disease on white clover seedlings, of which the symptoms were similar to those observed in the fields. Hence, their pathogenicity was confirmed on white clover. The infection rate of the fungal isolates varied from 78.5% to 95.2%, among which BWC98-105 was the most virulent isolate. The weeding efficacy of the fungus was maintained until the following year, leading to a significant reduction of reshooting. The fungus was specifically parasitic to white clover, but not to four lawn species including zoysiagrass (Zoysia japonica) under greenhouse test. The fungus also had no response to some Gramineae species including rice, but caused little damage to five species of Leguminosae.

Occurrence of Rice black-streaked dwarf fijivirus in Maize (옥수수의 벼검은줄오갈병)

  • Lee Bong-Choon;Hong Yeon-Kyu;Hong Sung-Jun;Park Sung-Tae
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.62-64
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    • 2006
  • July in 2005, we collected infected maize plant that showing stripe dwarf disease on maize leaf in Jeonbuk provinces including Gochang-gun and conducted genomic dsRNA extraction and RT-PCR. Genomic dsRNA was extracted directly in infected maize plant and electrophoresis in agarose gel. We confirmed 10 segments of genomic dsRNA. We conducted RT-PCR by genomic dsRNA and specific primer of S7, S8 and S10. As a result, specific band of expected size was confirmed respectively. In the results of dsRNA and RT-PCR analysis, we confirmed Rice black-streaked dwarf fijivirus (RBSDV) from naturally infected maize plant. Occurrence of RBSDV of maize plant was dealt 22 ha's damage in maize field. The occurrence rate was 80% in a lot of places of disease.

Construction of a High-Quality Yeast Two-Hybrid Library and Its Application in Identification of Interacting Proteins with Brn1 in Curvularia lunata

  • Gao, Jin-Xin;Jing, Jing;Yu, Chuan-Jin;Chen, Jie
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.108-114
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    • 2015
  • Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

A Study on Korean Traditional Food Use Patterns in Households of Middle Aged Women living in Kyunggido and Incheon Area (중년주부 가정의 전통음식 이용에 관한 연구 - 경기.인천 지역을 중심으로 -)

  • Kang, Up-Soon;Chyun, Jong-Hee
    • Journal of the Korean Society of Food Culture
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    • v.13 no.5
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    • pp.461-474
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    • 1998
  • The purpose of this study is to investigate the patterns of Korean traditional food use in the households of middle aged women living in Kyunggido and Incheon area Four hundred eighty seven housewives whose children were in middle school answered the questionnaire about Korean traditional food use patterns in ordinary days, national festive days and in private celebration or memorial days. Most households have used Korean traditional table setting called Bansang as daily meals(96.7%) and have used home-made Kimchi(97.3%). However, the use of home-made Jang such as soy sauce, soybean paste and red pepper soybean paste was not very high(64.3%). Most households have celebrated New Years day(99.6%) with Ddukguk and Manduguk, Chusuk(95.1%) with Songpyun, and fruits, Full-Moon day(95.1%) with Ogokbap, Namul and Brum. Dongji was celebrated with Patjug by 73.3% of households. However, Samjitnal and Danho were celebrated with Whajeon and Ssukjulpyun by only 18.9% and 43.8% of the households respectively. The households using Korean traditional food to celebrate the birthday, the 1st birthday of child, the wedding or to memorize the passing day of ancestors were 96.0%, 80.7%, 81.3% and 86.7% respectively. Compared to households of urban and nuclear family, households of rural and extended family tended to use significantly more Korean traditional foods in many occasions and showed significantly higher rate of using home-made Kimchi and Jang.

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A Survey on Elementary, Middle and High School Students' Attitude and Eating Behaviors about Kimchi in Seoul and Kyunggido Area (서울.경기지역 초.중.고등학생의 김치 섭취 실태 및 태도 조사)

  • Moon, Hae-Jin;Lee, Young-Mee
    • Journal of the Korean Society of Food Culture
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    • v.14 no.1
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    • pp.29-42
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    • 1999
  • This study was performed with 482 subjects(278 boys and 204 girls in Seoul and Kyunggido area) to find out the attitude and eating behaviors about Kimchi on Korean young generation. General characteristics about subject and attitude and eating behavior were surveyed by self-developed questionnaires. 85.9% of the subjects were nuclear family type and 49.9% of respondent's mother had full-time job and 96.1% of subjects were middle or upper economic status. The preference for Kimchi, 60.4% of respondents answered that they liked Kimchi. According to grade, the rate of preference about Kimchi was significantly different, higher grade students were found higher preference on Kimchi : 64.4% of middle and 70.4% of high school students liked Kimchi. The eating patterns of Kimchi, up to 60% of respondents answered they ate Kimchi in every meal time, which suggested that it was still an important side dish in Korean meal among teenagers. The eating behaviors on Kimchi, $85{\sim}90%$ of respondents answered that they preferred Kimchi in meal time. Baechu Kimchi(chinese cabbage Kimchi) and Gakdugi(radish cube Kimchi) was highly preferred. And their preference of food which made from Kimchi (such as Kimchi fried rice, Kimchi stew, Kimchi pancake) was highly evaluated. 65.6% of residents pointed out that 'Kimchi is very nutritious food'. The respondent's structure of value on Kimchi were not significantly difference between sex and age. But it was significantly difference(p<0.01) between the groups with preference and familiarity on Kimchi. The group had higher preference and familiarity on Kimchi agreed to the positive values about Kimchi. These result suggested that various kinds of Kimchi should be developed for young generation to meet their taste and also continuous nutrition education about traditional food especially Kimchi should be promoted at home and school.

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Effects of the Geometry and Location of an Vertical Opening on the Fire Characteristics in the Under-Ventilated Compartment Fire (환기부족 구획화재에서 수직 개구부의 형상 및 위치가 화재특성에 미치는 영향)

  • Mun, Sun-Yeo;Park, Chung-Hwa;Hwang, Cheol-Hong;Park, Seul-Hyun
    • Fire Science and Engineering
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    • v.27 no.3
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    • pp.20-29
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    • 2013
  • To investigate numerically the effects of geometry and location of vertical opening on the thermal and chemical fire characteristics in full-scale under-ventilated compartment fires, the ventilation factor ($A\sqrt{h}$) to estimate a theoretical maximum inflow of ambient air and the mass loss rate in a heptane pool fire were fixed for all cases. It was shown that variations in door geometry affected significantly the change in thermal and chemical characteristics inside the compartment. Variations in window location resulted in the complex change in additional fire characteristics including the fire duration time and recirculating flow structure. These results were analyzed in details by the multi-dimensional flow and fire characteristics including the vent flow and fuel/air mixing phenomena.

A Case of Multiple Recurred Mucinous Eccrine Carcinoma (수 차례 국소 재발한 피부 점액성 에크린 암)

  • Lee, Yoon-Jung;Lee, Kyung-Suk;Kim, Nam-Gyun;Kim, Jun-Sik
    • Archives of Plastic Surgery
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    • v.38 no.2
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    • pp.189-193
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    • 2011
  • Purpose: Mucinous eccrine carcinoma of the skin is rare skin adnexal malignant tumor, which most commonly occurs in the head and neck regions. This tumor, although rarely metastatic, has a high incidence of local recurrence. We report a case of mucinous eccrine carcinoma developed on the Lt. cheek which was treated by Mohs surgery. Methods: The patient was a 53-year-old man who showed a palpable skin-colored $1.5{\times}1.5cm$ sized mass on Lt. cheek. We treated it by wide excision, but it recurred several times on the same region. So we finally did the Mohs surgery and full thickness skin graft. Results: The histologic characteristics are large mucinous pools with fibrous septae and clusters of tumor cells. Special stains showed that the mucin was positive reactivity on Periodic acid Schiff. Also the immunocytochemical studies showed a positive reactivity to carcinoembryonic antigen, S-100 protein, cytokeratin-7. Therefore pathologist reported it as a mucinous eccrine carcinoma. Conclusion: Authors experienced a rare case of primary mucinous eccrine carcinoma on Lt. cheek which had recurred several times. Because of the high local recurrence rate, we did the Mohs surgery and got an good result. Therefore the early Mohs surgery would be a good choice to primary mucinous eccrine carcinoma.

Characteristics of the Electro-Optical Camera(EOC)

  • Lee, Seung-Hoon;Shim, Hyung-Sik;Paik, Hong-Yul
    • Proceedings of the KSRS Conference
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    • 1998.09a
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    • pp.313-318
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    • 1998
  • Electro-Optical Camera(EOC) is the main payload of Korea Multi-Purpose SATellite(KOMPSAT) with the mission of cartography to build up a digital map of Korean territory including Digital Terrain Elevation Map(DTEM). This instrument which comprises EOC Sensor Assembly and EOC Electronics Assembly produces the panchromatic images of 6.6 m GSD with a swath wider than 17 km by push-broom scanning and spacecraft body pointing in a visible range of wavelength, 510 ~ 730 nm. The high resolution panchromatic image is to be collected for 2 minutes during 98 minutes of orbit cycle covering about 800 km along ground track, over the mission lifetime of 3 years with the functions of programmable rain/offset and on-board image data storage. The image of 8 bit digitization, which is collected by a full reflective type F8.3 triplet without obscuration, is to be transmitted to Ground Station at a rate less than 25 Mbps. EOC was elaborated to have the performance which meets or surpasses its requirements of design phase. The spectral response the modulation transfer function, and the uniformity of all the 2592 pixel of CCD of EOC are illustrated as they were measured for the convenience of end-user. The spectral response was measured with respect to each gain setup of EOC and this is expected to give the capability of generating more accurate panchromatic image to the EOC data users. The modulation transfer function of EOC was measured as greater than 16% at Nyquist frequency over the entire field of view which exceeds its requirement of larger than 10%, The uniformity that shows the relative response of each pixel of CCD was measured at every pixel of the Focal Plane Array of EOC and is illustrated for the data processing.

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MBE growth of topological insulator $Bi_2Se_3$ films on Si(111) substrate

  • Kim, Yong-Seung;Bansa, Namrata;Edrey, Eliav;Brahlek, Mathew;Horibe, Yoichi;Iida, Keiko;Tanimura, Makoto;Li, Guo-Hong;Feng, Tian;Lee, Hang-Dong;Gustafsson, Torgny;Andrei, Eva;Cheong, Sang-Wook;Oh, Seong-Shik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.59-59
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    • 2011
  • We will report atomically sharp epitaxial growth of $Bi_2Se_3$ three-dimensional topological insulator films on Si(111) substrate with molecular beam epitaxy (MBE). It was achieved by employing two step growth temperatures to prevent any formation of second phase, like as $SiSe_2$ clusters, between $Bi_2Se_3$ and Si substrate at the early stage of growth. The growth rate was determined completely by Bi flux and the Bi:Se flux ratio was kept ~1:15. The second-phase-free atomically sharp interface was verified by RHEED, TEM and XRD. Based on the RHEED analysis, the lattice constant of $Bi_2Se_3$ relaxed to its bulk value during the first quintuple layer implying the absence of strain from the substrate. Single-crystalline XRD peaks of $Bi_2Se_3$ were observed in films as thin as 4 QL. TEM shows full epitaxial structure of $Bi_2Se_3$ film down to the first quintuple layer without any second phases. This growth method was used to grow high quality epitaxial $Bi_2Se_3$ films from 3 QL to 3600 QL. The magneto-transport properties of these thin films show a robust 2D surface state which is thickness independent.

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