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http://dx.doi.org/10.5423/RPD.2006.12.1.062

Occurrence of Rice black-streaked dwarf fijivirus in Maize  

Lee Bong-Choon (Yeongnam Agricultural Research Institute, NICS, RDA)
Hong Yeon-Kyu (Yeongnam Agricultural Research Institute, NICS, RDA)
Hong Sung-Jun (Yeongnam Agricultural Research Institute, NICS, RDA)
Park Sung-Tae (Yeongnam Agricultural Research Institute, NICS, RDA)
Publication Information
Research in Plant Disease / v.12, no.1, 2006 , pp. 62-64 More about this Journal
Abstract
July in 2005, we collected infected maize plant that showing stripe dwarf disease on maize leaf in Jeonbuk provinces including Gochang-gun and conducted genomic dsRNA extraction and RT-PCR. Genomic dsRNA was extracted directly in infected maize plant and electrophoresis in agarose gel. We confirmed 10 segments of genomic dsRNA. We conducted RT-PCR by genomic dsRNA and specific primer of S7, S8 and S10. As a result, specific band of expected size was confirmed respectively. In the results of dsRNA and RT-PCR analysis, we confirmed Rice black-streaked dwarf fijivirus (RBSDV) from naturally infected maize plant. Occurrence of RBSDV of maize plant was dealt 22 ha's damage in maize field. The occurrence rate was 80% in a lot of places of disease.
Keywords
Genomic dsRNA; Rice black-streaked dwarf fijivirus; RT-PCR;
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1 Fang, S., Yu, J., Feng, J., Han, C., Li, D. and Liu, Y. 2001. Identification of Rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Arch. Virol. 146: 167-170   DOI
2 Shikata, E. and Kitagawa, Y. 1977. Rice black-streaked dwarf virus: its properties, morphology, and intracellular localization. Virology 77: 826-842   DOI   ScienceOn
3 Wang, Z. H., Fang, S. G, Xu, J. L. Sun, L. Y., Li, D. W. and Yu, J. L. 2003. Sequence analysis of the complete genome of Rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes 27: 163-168   DOI   ScienceOn
4 Lee, M., H., Heo, C. H., Park, K. B. and Lee, Y. S. 1989. Productivity of silage by chemical control under different planting date in the Rice black-streaked dwarf virus of com. Res. Rept. RDA 31: 31-35
5 Uyeda, I., Lee, B.-C. and Ando, Y. 1998. Reovirus isolation and RNA extraction. In: Foster GD, Taylor SC (eds). Methods Mol. Bioi. 81: 65-75
6 Lee, B. C, Hong, Y. K., Hong, S. J., Park, S. T. and Lee, K. Y. 2005. Occurrence and detection of Rice black-streaked dwarf virus in Korea. Plant Pathol. J. 21: 172-173   과학기술학회마을   DOI   ScienceOn
7 Zhang, H. M., Chen, J. P. and Adams, M. J. 2001. Molecular characterisation of segments 1 to 6 of Rice black-streaked dwarf virus from China, provides the complete genome. Arch. Virol. 146: 2331-2339   DOI
8 Isogai, M., Uyeda, I. and Choi, J. K. 2001. Molecular diagnosis of Rice black-streaked dwarf virus in Japan and Korea. Plant Pathol. J. 17: 164-168