• Journal of the Korean Society of Tobacco Science
• /
• v.10 no.1
• /
• pp.57-63
• /
• 1988

The mutagenic activities of the pyrolysates(300, 600, 750 and 85$0^{\circ}C$) of extracts from four antitussive or expectorant medicinal plants(Daturae Folios, Scopoliae Rhizoma, Acori Rhisoma, and Schigandrae Fructus) were studied in the Ames Salmonella/microsomes test system. The pyrolyzate of Schizandrae Fructus did not show the mutagenicity to tester strain TA98 of Salmonella typhinurium. However, those of other medicinal Plants, Daturae Folium, Scopoliae Rhigoma and Acori Rhizoma, exhibited the mutagenic activity to this strain and the mutagenicity of them were decreased gradually to 70% of the initial activity according to time course after the preparation of the samples with DMSO.

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.737-740
• /
• 2000

Two groups of bacteria, harmful and useful bacteria, coexist in our body. Harmful bacteria and free radicals which cause many diseases and agings could be controlled by not synthetic materials but natural products. 50 kinds of natural products were searched for this purpose, some of them expressed bacterial controlling ability. Clostridium difficle was controlled by Paeonia Japonica and Lycii Fructus, etc., Clostridium perfringens was inhibited by Scutellaria Baicalensis and Astragali Radix, etc., and same way, Listeria monocytogenes; Paeonia Japonica and Scutellaria Baicalensis, E. coli; Scutellaria Baicalensis and Schizandrae Fructus, Staphylococcus aureus; Paeonla Japonica and Scutellaria Baicalensis, Streptococcus mutans; Coptis Rhizoma, respectively. These natural products will play an important role in bio-Industry.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.4
• /
• pp.848-859
• /
• 2005

The following conclusions are drawn, through studying of referring literature, of contents of the prescription and of clinical applications in Hyungsang Medicine. Insamyangyung-tang(인삼양영탕) was appeared first in Taepunghyemin-whajegugbang(태평혜민화제국방) which was written by Jin and others at Song Dynasty. This prescription is applied to senility and long lasting weakness, little strength of spleen and lung(비폐기허), and insufficiency of blood. The Insamyangyung-tang is composed of medical stuffs which Rhizoma Cnidii is taken out from Sipjiendaebotang and Pericarpium Citri Nobilis, Fructus Schizandrae, Radix Polygalae are added to it. This prescription is used in China up to the present. In korea, according to Donguibogam, Rhizoma Cnidii and Poria are taken out from Sipjiendaebotang and Pericarpium Citri Nobilis, Fructus Schizandrae, Radix Polygalae are added to it, and Ramulus Cinnamomi is changed to Cortex Cinnamomi, and also Radix Ledebouriellae is newly added to it. This is reorganized to fit for Korean physical conditions and to be useful for deficiency of blood, and also to be efficacious for coming and going of chillness and fever and running with sweat due to weakness of Liver. In the point of view of Hyunsang medicine, Insamyangyung-tang is applied to shapes and symptoms as follows : This prescription becomes more efficacious to women than to men. At the age of fifties when liver grows weak it effects a cure. It takes effect to the shape of going easily into the deficiency of blood, that is, to the Hyul Kwa with an oval face. It effects a cure on the man who has wrinkles on the bridge of the nose, who has marks of being choked up on the central area between two brows, or who has distinctive size of eyes and nose. It is efficacious against symptoms which are to be exhausted, to be weak, to lose flesh, to be coming and going of chillness and fever and running with sweat.

• Applied Biological Chemistry
• /
• v.37 no.1
• /
• pp.30-36
• /
• 1994

The essential oil of Schizandrae Fructus was extracted by simultanous distillation-extraction and steam distillation and analyzed by GC and GC-MS. The oil content of Schizandrae Frutus was 0.64% when extracted by steam distillation and 0.88% in case of simultanous distillation extraction and sensory analysis of the oil indicated woody, camphorous, spicy and sour characteristic notes. The experimental results confirmed the presence 47 volatile components, the major components were ${\gamma}-terpinene$, p-cymene, ${\alpha}-ylangne$ and ${\beta}-elemene$. The oil was fractioned into ten fractions and 4 fraction of them have a good aroma character. The application of the oil showed the utilization possibility as flavoring materials for Korea tea.

• Journal of Haehwa Medicine
• /
• v.10 no.2
• /
• pp.31-40
• /
• 2002

According to the literatural study on the utility of TaeWonEum, the results were as follows 1. TaeWonEum is the prescription originally registered on Zang jie Bin's ${\ll}$Jing-Yue-Quan-Shu${\gg}$ and because of the dificiency of the renmai, the chongmai in women, it is made for the discomfort of the pregnancy 2. On the treatment of miccarriage, they were used to the methods of tonificating qi and blood, tonificating insufficiency, making flesh, eliminating wind, tonificating the spleen, clearing heat, eliminating stagnant blood, tonificating blood, growing zhang qi, etc but it is almostly used to the method of tonificating qi and blood 3. TaeWonEum is used on the purposes of tonificating the spleen and stomach, qi and blood, on the case of both the discomfort of the pregnancy by the dificiency of the renmai, the chongmai in women, and the insufficiency of fetal growth by the difficiency of the spleen and stomach, qi and blood 4. The symptoms can be used which are the fetal problem of incipient pregnancy, quickening, lumbago, distension, pallor, weakness of the mind, dizziness, cold, anorexia 5. The additional methods of TaeWonEum are as follows when much turbid enuresis is concerned, Disscoreae Rhizoma, Psoraleae Fructus, Schizandrae Fructus can be added when a patient is very difficient of qi, Atractylodis Macrocephalae Rhizoma is doubled and Astragali Radix can be added when a patient is often vomiting with asthenia-cold, Typhae Pollen can be added when a patient has fever and weak, Scutellariae Radix or Rehmanniae Radix can be added and E ucommiae Cortex can be subtracted when a patient has the colicky pain because of the difficiency of yin, Lycii Fructus can be added when a patient is at a great rage and qi is ascenting, Cyperi Rhizoma or Amomi Fructus can be added when a patient has hemorrage with trauma, Dipsaci Radix, Asini Gelatinum can be added when a patient's vomiting can not be reduced, Pinelliae Rhizoma, Zingiberis Rhizoma Recens can be added

• KSBB Journal
• /
• v.18 no.4
• /
• pp.282-288
• /
• 2003

For the screening of anti-inflammation and antioxidative activities, ethanolic extract of 40 species of traditional herbal medicines were examined their hyaluronidase inhibitory effect and radical scavenging activity in vitro. From the result of the hyaluronidase inhibitory activity using a Morgan-Elson assay, Astragali Radix, Eucommia Cortex, Schizandrae Fructus, Scutellaria Radix, Acanthopanacis Cortex, Chaenomelis Fructus, Amomum xanthioides Wallich and Moutan Radicis Cortex showed more than 50% hyaluronidase inhibitory effects at the concentration of 10 mg/mL. In the various solvent fractions (n-hexane, chloroform, ethyl acetate, water) prepared from ethanolic extracts, the ethyl acetate fraction of all extracts tested showed strong activity. Antioxidative activity was evaluated by assaying electron-donating ability to DPPH free radical and scavenging of hydroxyl radical (ㆍOH) generated through Fenton reaction, respectively. Rubus coreanus Miq, Moutan Radicis Cortex, Paeoniae Radix Alba, Plantaginis Semen and Sorbus commixta Hedl. showed high activity more than 90%, yet similar activity to $\alpha$-tocopherol and BHA at the concentration of 1 mg/mL in electron donating activity. The scavenging effects of ethanolic extracts on hydroxyl radical were investigated using a 2-deoxyribose oxidation method and tested all extracts showed significant radical scavenging activity. The experiment was also performed to examine whether herbal medicines having significant lipid peroxidation inhibitory activity, Schizandrae-Fructus is the strongest inhibitory activity in both linoleic acid and liposome peroxidation.

• Korean Journal of Pharmacognosy
• /
• v.35 no.4 s.139
• /
• pp.276-282
• /
• 2004

This study has been conducted to investigate the amount of residual sulfur dioxide for herbal medicines of 30 species which are purchased in 13 different regions (Bonghwa, Busan, Chunju, Jecheun, Kwangiu, Keumsan, Seoul, Taeku, Cheungdu, Xian, Beijing, Tokyo and Osaka). The sulfur dioxide residues were determined in the collected 386 samples by the modified Monier-Williams method. The residues of sulfur dioxide in 386 samples ranged from ND (under detection limit) to 2808 ppm. The sulfur dioxide in Cassiae Semon, Cinnamomi Ramulus, Cervi Cornu, Hoelen, Crataegi Fructus, Artemisiae Argyi Herba, Scolopendrae Corpus, Schizandrae Fructus and Cyperi Rhizoma were detected under the detection limit regardless of the collected regions. The sulfur dioxide residues in herbal medicines collected in domestic regions were relatively lower than those in foreign regions. There are no standards for sulfur dioxide residual limits of herbal medicines in Korea. This results will provide the scientific basis for the standardization of sulfur dioxide residues in Korea Pharmacopoeia.

• Microbiology and Biotechnology Letters
• /
• v.30 no.3
• /
• pp.270-281
• /
• 2002

The growth of Enterobacteria, Lactobacillus acidophilus and L. plantarum was promoted by natural products themselves bearing antioxidative capacity and combined two, three and four kinds of them.1. acidophilus was promoted by Paeonia japonica, Acori graminei rhizoma, Aurantii nobilis pericarpium, alone, and two mixed combinations were composed of Acori graminei rhizoma and Cinnamomi cortex, Aurantii nobilis pericarpium and Puerariae radix, and three mixed combinations were formed with Cimicifugae rhi- zoma, Cinnamomi cortex and Glycyrrhizae radix, and four mixed combinations were made up Glycyrrhizae radix, Theae folium, Scutellaria baicalensis george and Cinnamomi cortex. There four combination promoted growth of 1. acidophilus with 1.3 times than that of control, and their antioxidative capacity also higher than that off times, the ratio of elimination of hydroxyl radical was more than 70% in dilution rate of 100 times. In the case of 1. plantarum was premoted by Corm fructus, Nelumbo nuclfera gaertner Sophorae flos, alone, and two mixed combinations were organized with Schizandrae Iructus and Theae folium, Paeonia japonica and Epimedii herba, and three mixed combinations were combined with Corni fructus and Theae folium and Paeonia japonica, Corni fructus and Coptidis rhizoma and Schieandrae fructus, and four mixed combinations were composed of Corm fructus, Theae folium, Coptidis rhizoma, Scutellaria baicalensis george, and Corm fructus, Schizandrae fructus, Paeonia japonica, Theae folium. These four combinations assisted growth of 1. plantarum with 1.5 times than that of control, and their antioxidative capacity also higher than that of 4-5 times, the ratio of elimination of hydroxyl radical was more than 75% in dilution rate of 100 times. As these combinations of natural products could activate some parts of body, they might be applied pharmaceuitcal sources, functional foods, and expected to fermentative beverages.

• Korean Journal of Food Science and Technology
• /
• v.24 no.6
• /
• pp.597-602
• /
• 1992

To improve utilization of boxthorn (Lycii Fructus), the boxthron and mixed boxthron (Lycii Fructus, Schizandrae Fructus, Corni Fructus, Zizyphi Fructus, Zingiberis Rhizoma, Cinnamami Cortex) hot-water extractable concentrates were prepared by vaccum evaporation and its rheological properties were investigated. The rheological properties of concentrates $(20{\sim}50^{\circ}Bx)$ followed power low model and showed a pseudoplastic behavior at the temperature range of $20{\sim}60^{\circ}C$. The apparent viscosity of $20^{\circ}Bx$, $30^{\circ}Bx$, $40^{\circ}Bx$ and $50^{\circ}Bx$ boxthron hot-water extractable concentrate was 0.0074 Pa s, 0.0175 Pa s, 0.0431 Pa s and 0.0988 Pa s, that of mixed boxthorn hot-water extractable concentrate was 0.0099 Pa s, 0.0328 Pa s, 0.0720 Pa s and 0.1940 Pa s at $20^{\circ}C$ and 1500 l/s, respectively. The yield stress of boxthron and mixed boxthron hot-water extractable concentrates ranged from 0.045 to 6.253 Pa and from 0.022 to 8.891 Pa, respectively. The activation energy for the flow of boxthron and mixed boxthorn hot-water extractable concentrates increased from 1.6182 to $2.0543{\times}10^7\;J/kg{\cdot}mol$ and from 1.7057 to $2.1462{\times}10^7\;J/kg{\cdot}mol$ with the concentrations of concentrates, respectively.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.26 no.3
• /
• pp.330-337
• /
• 2012

This study was performed to assess the Effect of SCF(Schisandrae Chinensis Fructus) on Keratinocyte Damage by UV irradiation. The effect of SCF were determined in UV irradiated HaCaT. We measured LDH release and NO release from HaCaT to elucidate the effect of SCF. And iNOS, TNF-${\alpha}$, COX-2, Bax, Bcl-2, Bcl-xL, c-jun, c-fos gene expression were determined in HaCat using real time PCR method. The results are as follows. SCF inhibited LDH-release, NO production in UV irradiated HaCaT. SCF increased the gene expression Bax, Bcl-2 and Bcl-xL protein in UV irradiated HaCaT. SCF suppressed the gene expression TNF-${\alpha}$ in UV irradiated HaCaT. SCF suppressed the gene expression iNOS, c-fos, and c-jun in UV irradiated HaCaT. SCF not affected the suppression of the gene expression COX-2 in UV irradiated HaCaT. The study showed SCF inhibited the cell damage in UV irradiated HaCaT.