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Effect of Schisandrae Chinensis Fructus on Keratinocyte Damage by UV Irradiation  

Park, Hyung-Hoon (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee, Jang-Suk (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
Yun, Hyuk (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Hwang, Gwi-Seo (Department of Preventive Medicine, College of Oriental Medicine, Gachon University)
Chong, Myong-Soo (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.26, no.3, 2012 , pp. 330-337 More about this Journal
Abstract
This study was performed to assess the Effect of SCF(Schisandrae Chinensis Fructus) on Keratinocyte Damage by UV irradiation. The effect of SCF were determined in UV irradiated HaCaT. We measured LDH release and NO release from HaCaT to elucidate the effect of SCF. And iNOS, TNF-${\alpha}$, COX-2, Bax, Bcl-2, Bcl-xL, c-jun, c-fos gene expression were determined in HaCat using real time PCR method. The results are as follows. SCF inhibited LDH-release, NO production in UV irradiated HaCaT. SCF increased the gene expression Bax, Bcl-2 and Bcl-xL protein in UV irradiated HaCaT. SCF suppressed the gene expression TNF-${\alpha}$ in UV irradiated HaCaT. SCF suppressed the gene expression iNOS, c-fos, and c-jun in UV irradiated HaCaT. SCF not affected the suppression of the gene expression COX-2 in UV irradiated HaCaT. The study showed SCF inhibited the cell damage in UV irradiated HaCaT.
Keywords
schizandrae chinensis fructus; HaCaT keratinocyte; UV irradiation;
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