• Journal of fish pathology
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• v.2 no.2
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• pp.99-108
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• 1989

Anaethetics are needed for handling fish, especially for transportation, tagging and grading. Among them, MS-222 has been popular in aquaculture since it has an excellent anaesthetic effect. However, MS-222 is more expensive than other chemicals. Benzocaine (Ethyl-p-aminobenzoate) has a similar molecular formula and equivalent anaesthetic effect to MS-222, and is cheaper. The purpose of this study was first to compare anaesthetic effects (Benzocaine) under various conditions : temperature, concentration, pH and body weight. Second purpose was to compare actual anaesthetic effects at 50 ppm benzocaine at ambient temperature and pH for the grading of tilapia. The results of this study are as follows : 1. The effect of anaesthesia at $24^{\circ}C$ was better with low pH, that is 5.6 than high pH 6.6 and 7.6. 2. The anaesthetic effect was not different at different body weight form 11g to 1,350g. 3. The fish were anaesthetized in 4~10 minutes at 50 ppm benzocaine at temperature. $20{\sim}24^{\circ}C$ and pH 6.8~7.3 and recovered in 4~6 minutes when they were put back in the fresh water after 30 minutes anaesthesia. 4. Benzocaine was more sensitive at pH fluctuation than temperature. 5. Twenty four hour-TLm of Benzocaine was 50 ppm at $24^{\circ}C$, pH 6.8 when the fish were put back in the fresh water after 120 minutes.

• Journal of fish pathology
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• v.16 no.2
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• pp.119-123
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• 2003

We have selected an valuable pmbiotic strain; Lactobacillus sakei BK19 which has wide antagonic spectrum against fish pathogens . Present study investigated cultural characterization of L. sakei BK19 including pH tolerance , susceptibility of antibacterial agents and growth pattern with different environment such as nutritions, temperature and salinity. L. sakei BK19 showed Significantly higher resistance at low pH(around pH 4) environment and relative high antibiotic tolerance . In the study of optimal culture condition, maltose and saccharose provided the optimal nutritional culture condition while lactose and mannitol were unable to supply its carbon source for the fermentation of L. sakei BK19. Moreover. L. sakei BK19 showed good growth at the temperature range of 15 to $45^{o}C$ und the NaCl concentration of 0 to 7%. Hence, this particular probiotic strain may be benificial both in seawater and fresh weter conditions.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.6
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• pp.619-626
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• 2012

This study investigated the food quality characteristics of mottled skate Raja pulchra including the proximate composition, taste, color, flavor and texture as affected by area caught (Heuksando and Daecheongdo), sex and fish weight (heavy, intermediate and light grades). Based on viable cell counts, all of the mottled skates studied remained fresh. The moisture and protein contents of the mottled skate did not differ by the area caught and fish weight, but differed by fish sex. The taste of mottled skate was affected only by fish weight. The texture of the mottled skate differed according to fish sex and fish weight. The odor intensity did not differ for the three parameters.

• Parasites, Hosts and Diseases
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• v.22 no.2
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• pp.190-202
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• 1984

In an attempt to clarify the epidemiological feature of distomiasis in Tongjin riverside area, the prevalence of distomiasis in the residents and infection rates of the metacercariae in fresh-water fishes were investigated at the upper, middle and lower reaches of the river from January to April, 1984. The results obtained were summarized as follows: 1. Out of a total of 931 fresh-water fishes which composed of 33 different species, 611 fishes(65.6%) of 31 species were found positive with digenetic trematode metacercariae of 16 different species, and there were some differences in infection rates of the metacercariae among the fishes in the 3 parts of the river; 53.8% in upper, 80.7% in middle, and 61.0% in lower reaches, respectively. 2. Infection rates of the metacercariae of Exorchis oviformis, Metagonimus yokogawai, Echinochasmus japenicus, Metorchis orientalis and Clonorchis sinensis in the fishes were 48%,29%, 115, 7.9% and 6.3oA, respectively. 3. The average number of the encysted larvae of Clonorchis found in fish body/gram showed 4.44 in Pseudorasbera larva, Gnathepegon coreanus (1.2), Microphysogoio yaluensis (0.76), Abbottina springeri (0.4), Acanthorhodeus asmussi (0.21) and Cultriculus eigenmanni (0.17), respectively. 4. The average number of the metacercariae of Metagonimus found in fish body/gram disclosed 34.01 in Zacco platypus, Zacco temmincki (16.46), Carassius carassius (5.35), Moroco oxycephalus (1.54) , Aphyocypris chinensis (1.5) and etc., respectively. 5. Detection rates of the eggs of Clonorchis and Metagonimus among residents were 1.1% and 0.8%, respectively, out of a total 923 Persons.

• Parasites, Hosts and Diseases
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• v.41 no.2
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• pp.117-120
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• 2003

A human Echinostoma hortense infection was diagnosed by gastroduodenoscopy. An 81-year-old Korean male, living in Yeongcheon-shi, Gyeongsangbuk-do and with epigastric discomfort of several days duration, was subjected to upper gastrointestinal endoscopy. He was in the habit of eating fresh water fish. Two live worms were found in the duodenal bulb area and were removed using an endoscopic forcep. Based on their morphological characteristics, the worms were identified as E. hortense. The patient was treated with praziquantel 10 mg/kg as a single dose. The source of the infection in this case remains unclear, but the fresh water fish consumed, including the loach, may have been the source. This is the second case of E. hortense infection diagnosed by endoscopy in Korea.

• The Korean Journal of Pharmacology
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• v.11 no.1 s.17
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• pp.15-18
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• 1975

The effect of skin extracts of Korean amphibian, poisonous snake and fresh-water fish were determined for their caerulein-like action on rabbit gall bladder strips. The isolated gall bladder strips were prepared according to the technique described by Amer and Becvar(1969). The strips were placed in a bath containing 100ml of Locke-Ringer solution maintained at $38^{\circ}C$. Oxygen was continuously bubbled through the solution. The tension of the muscle strip was initially adjusted to 0.7g. The contractile response was measured isometrically by a force-displacement transducer connected to a polygraph. In this rabbit gall bladder strip caerulein produced contraction of CCK-PZ type. The skin extract of Korean amphibian also elicited similar contraction as caerulein, which extracted from Australian amphibian, Hyla caerulea, by Erspamer et al. The calculated amount was approximately $2{\mu}g$ caerulein per gram of skin tissue in Korean amphibian and the potency was about 1/200 of that seen in Australian amphibian. The contraction of gall bladder strip by our amphibians occurs in decreasing order; Rana Nigromaculata coreana Okada, Rana nigromaculata Hallowell, Hyla arborea japonica Gunther and Bombina orientalis Boulenger. The skin extracts of poisonous snake and fresh-water fish produced no caerulein-like activity.

• Journal of fish pathology
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• v.30 no.1
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• pp.25-39
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• 2017

A total number of 668 apparently healthy fish were obtained from farm to study the effect of two heavy metals in a form of (Copper sulfate and Mercuric chloride) on some hematological and biochemical parameters of blood. The $LC_{50}$ /96 hr. of Cu and Hg were estimated and fish exposed to $\text\tiny{^1/_2}$ $LC_{50}$ for 7 days and for $1/_{10}$ $LC_{50}$ for 8 weeks from each product separately. Results showed decrease in RBCs count, PCV% and Hb in acute and chronic mercury while a significant increase was shown in acute and chronic copper toxicity, total leucocytic count showed decrease in acute mercury toxicity and increase in the chronic case, while in copper toxicity non-significant decrease in acute and significant decrease in chronic toxicity was noticed. Elevated serum urea and creatinine in both acute and chronic mercury and copper toxicity was detected. No changes in total bilirubin in the acute mercury and chronic copper toxicity while significant increase in chronic mercury and acute copper. Elevation of serum AST and ALT in some days of acute toxicity of mercury and copper while in chronic mercury toxicity a significant elevation of both serums AST and ALT were detected .while in chronic copper toxicity serum AST was fluctuated and ALT showed no significant changes. CK study revealed significant decrease in acute mercury with fluctuation in the chronic toxicity while in copper toxicity it showed fluctuation in acute and significant decrease in chronic toxicity. Glucose value decreased in acute and chronic mercury toxicity while in copper toxicity it showed significant increase in the acute and increase followed by significant decrease in the chronic copper toxicity.

• Ecology and Resilient Infrastructure
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• v.10 no.4
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• pp.201-207
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• 2023

Suspended solid in river is resource serious contamination. Suspended solid have a pernicious influence to fish and aquatic ecosystem. Flood and sediment and land use in river are largely[mainly] responsible of suspended solid. Especially, resuspension phenomenon of sediment will be dangerous effect to aquatic ecosystem. Study of fish and aquatic ecosystem on suspended solidus slight in fresh water. This study was basic research to manage fish and aquatic ecosystem on turbidity. It was conducted to analyze and monitor fish response on turbidity. Result in this study was confirmed the insensitive and die of fish on reduction of oxygen supply.

• Journal of Animal Environmental Science
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• v.16 no.3
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• pp.215-226
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• 2010

The pig slurry leachate was dark brown-colored solution that leaches out of woodchip trickling filter. The purpose of this research was to investigate the effect of pig slurry leachate and byproduct on growth characteristics of leaf lettuce in hydroponics culture. The effects of addition of fish meal, bone meal and crab shell for the growth of leaf lettuce were investigated. Leaf lettuce were grown in each of six combination treatment solutions; slurry leachate, slurry leachate + fish meal, slurry leachate+bone meal, slurry leachate + crab shell and chemical hydroponic solution for lettuce based on EC content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of lettuce. The all of nutrient solution was adjusted 1.5 mS/cm in EC in hydroponics culture. 1. The pH level of leachate of trickling filter was increased and EC decreased gradually during treatment. Pig slurry leachate was low in suspended solids (SS), phosphorus (P), but rich in potassium (K). 2. The plot of slurry leachate (SL) was lowest in the growth characteristics of lettuce. The leaf length and width of lettuce treated with mixture plot of slurry leachate and fish meal (SL + FM) was higher compared with plot in slurry leachate. The chlorophyll reading was reduced in plot treated with slurry leachate, but that in plot of SL+FM was similar compared with control plot. 3. The fresh weight of lettuce showed lowest in the plot treated with slurry leachate. The addition of fish meal increased the yield of comparing plot of slurry leachate, but plots of bone meal and crab shell addition were not significantly difference. The fresh weight of leaf lettuce in plot of SL+FM was 87% as 400.0g compared with control. In conclusion, the mixture solution of pig slurry leachate and fish meal could be used as a nutrition solution of organic lettuce hydroponics.

• Food Science of Animal Resources
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• v.35 no.1
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• pp.71-79
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• 2015

This study was performed to determine the effect of multilayered fish oil (FO) emulsion without or with trans-cinnamal-dehyde on pork patties. Multilayered FO (-primary, -secondary, -tertiary) emulsions were prepared using a layer-by-layer deposition technique with Tween 20, chitosan, and low methoxyl pectin, and were added to pork patties at the same concentration. Pork patties were then stored for 20 d in a refrigerator ($5^{\circ}C$) to study changes in quality. The results showed that the pH value of all samples significantly decreased but cooking loss increased during storage (p<0.05). However, water-holding capacity and moisture content showed no remarkable difference between treatments and storage periods (p>0.05). All pork patties containing multilayered FO (treated samples) showed higher values for lightness and significantly lower values for yellowness compared to control pork patties (untreated sample). Lipid oxidation was higher in treated pork patties than in control pork patties during storage. In addition, lipid oxidation and total viable bacterial count in pork patties decreased as the number of coating layers increased. However, hardness, cohesiveness, and springiness of all samples showed no significant change during storage (p>0.05) as compared to fresh pork patties. Furthermore, these did not remarkable change with addition of trans-cinnamaldehyde in all pork patties. From our results, we suggest that FO emulsion did not affect the texture characteristics of fresh pork patties, indicating that it could be used to improve the quality of pork patties by contributing high-quality fat such as unsaturated fatty acids.