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Effect of Cycloheximide and Holding Solution on Vase Life of Cut 'Blue Magic' Iris Flowers according to the Flower Development and Opening Stages (Cycloheximide 및 보존용액 처리가 개화단계에 따른 절화 아이리스 '블루매직'의 수명에 미치는 영향)

  • Kim, Young-A;Lee, Jong-Suk
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.790-795
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    • 2010
  • This experiment was conducted to clarify the effects of cycloheximide and holding solution on vase life of cut 'Blue Magic' iris. The vase life of iris flowers held in 3% sucrose (S) + $100mg{\cdot}L^{-1}$ hydroxy quinoline sulfate (HQS) + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ Benzylaminopurine (BA), 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ cycloheximide (CHI), or 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI were much longer than those held in distilled water. Squeeze stem phenomenon that showed at a holding solution containing $200mg{\cdot}L^{-1}$ HQS disappeared at a holding solution containing $100mg{\cdot}L^{-1}$ HQS. The holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA extended the most effective treatments on vase life, fresh weight, water balance, and flowering of cut iris flowers. However, the holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI and 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI was not effective in solution uptake or transpiration, but did result in high water balance. Iris flowers treated with CHI at the half-open flower stage showed increases in ornamental value, such as full flower opening and extended vase life. To improve flower quality and prolonging vase life of cut iris flowers, a holding solution containing $50{\mu}M$ CHI can be used continuously from the half-open stage.

Analyses of CO2 Concentration and Balance in a Closed Production System for King Oyster Mushroom and Lettuce (밀폐형 식물생산시스템 내 새송이 버섯과 상추의 혼합 재배 비율에 따른 CO2 농도 변화 및 균형 분석)

  • Jung, Dae Ho;Kim, Chan Kyo;Oh, Kyung Hun;Lee, Dong-Hyeon;Kim, Minsu;Shin, Jong Hwa;Son, Jung Eek
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.628-635
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    • 2014
  • The large amount of $CO_2$ emitted from mushrooms during incubation and developmental stages can be utilized in plant production systems as a $CO_2$ source. The objectives of this study were to measure the $CO_2$ emission and absorption rates of mushroom and lettuce, respectively, and to analyze the $CO_2$ concentrations at various ratios of mushroom and lettuce in a closed production system. The $CO_2$ emission rate of king oyster mushrooms (Pleurotus eryngii ( DC.) Qu$\acute{e}$l) and $CO_2$ absorption rate of lettuces (Lactuca sativa L. cv. Asia Heuk Romaine) were measured by using two closed acryl chambers ($1.0m{\times}0.8m{\times}0.5m$) in which indoor temperatures were maintained at $18^{\circ}C$ and $22^{\circ}C$, respectively. The lettuce was grown at a light intensity of PPF $340mol{\cdot}m^{-2}{\cdot}s^{-1}$ and with nutrient solution at EC $1.2dS{\cdot}m^{-1}$. The air was periodically circulated between the two chambers using a diaphragm pump. The $CO_2$ emission rate of the mushroom increased until the $15^{th}$ day after scratching (DAS) and then decreased. The rate also increased with increased indoor temperature. In particular, the $CO_2$ emission rate per fresh weight of fruit body increased by about 3.1 times after thinning compared to before thinning. In terms of $CO_2$ balance, the $CO_2$ emission rates from a bottle (950 mL) of the mushroom at 9, 12, and 14 DAS were equivalent to those of 3, 4.5, and 5.5 lettuce plants at 7, 10, and 12 DAT (days after transplanting), respectively. This work shows that balance in $CO_2$ concentration could be achieved using an appropriate ratio of the two crops in a closed production system.

Effects of Differentiated Temperature Based on Growing Season Temperature on Growth and Physiological Response in Chinese Cabbage 'Chunkwang' (고랭지 여름배추 주산지의 기온을 기준으로 한 수준별 온도가 배추 '춘광'의 생육 및 생리반응에 미치는 영향)

  • Son, In-Chang;Moon, Kyung Hwan;Song, Eun Young;Oh, Soonja;Seo, Hyeongho;Moon, Young Eel;Yang, Jinyoung
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.3
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    • pp.254-260
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    • 2015
  • Changes of the growth, quality and physiological response of Chinese cabbage cv 'Chunkwang' in response to five different temperature treatments based on climate change scenario were investigated during the growing season. The treatments consisted of normal year temperature $-2.0^{\circ}C$ (I), normal year temperature (II; Control group), normal year temperature $+2.0^{\circ}C$ (III), normal year temperature $+4.0^{\circ}C$ (IV), and normal year temperature $+6.0^{\circ}C$ (V). Regarding fresh weight, number of leaves, and leaf area were high in group IV, and V before the head formation stage, but it has decreased during the later growth period. Rate of frangibleness sympton was the highest in group V as 85.7%, and it was decreased in group IV (64.3%), group III (28.6%), group II (14.3%), and group I (7.1%). Regarding photosynthetic rate, group III, IV, and V showed relatively high photosynthetic rate at 20 DAP but it was reduced dramatically during the later growth period. Transpiration and stomatal conductance showed the similar trend with the photosynthetic rate. When comparing the chlorophyll fluorescence reaction of each treatment group at 50 DAP, Fv/Fm in group I was highest as 8.04 among all treatment groups and the lowest in group IV as 7.15.

Quality characteristics of kimchi prepared with cut Kimchi cabbages during the short-term storage (단기저장 절단배추 김치의 품질 특성)

  • Son, Eun Ji;Kim, Sang Seop;Chung, Shin-Kyo
    • Food Science and Preservation
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    • v.24 no.2
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    • pp.215-220
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    • 2017
  • Cut Kimchi cabbages ($3{\times}3cm$) were dipped in the egg shell solution (0.5% egg shell calcium/0.5% citric acid solution) and stored in the low-density polyethylene (LDPE) film bag at $4^{\circ}C$ for 2 weeks. Using this cut Kimchi cabbage, kimchi was prepared and their physicochemical qualities were investigated. Moreover, their sensory qualities were compared with Kimchi prepared with normal Kimchi cabbages. Egg shell calcium pretreatment (ET) showed the lower weight loss of cabbages than non-treatment (NT), and soluble solid compounds were decreased in all samples. Titratable acidity showed no statistical difference. After making a kimchi using cut Kimchi cabbages stored for 2 weeks no statistical differences in soluble solids and titratable acidities of kimchi stored for 7 days were shown. As a result of sensory test, preference of color was decreased and salted condition of control was the most significantly decreased. Pickled seafood odor of kimchi showed statistical difference, compared with the control. Crispness decreased in all samples. On the other hand, salty flavor and pickled seafood flavor were increased, fresh cabbage flavor, bitter flavor and carbonic flavor were decreased. Overall sensory quality of cut Kimchi cabbage (ETK) didn't show significant difference compare with kimchi prepared with normal cabbage (CON). It is possible to make kimchi with approvable sensory quality using cut Kimchi cabbage treated with egg shell calcium.

Mass Culture and Ginsenoside Production of Ginseng Hairy Root by Two-Step Culture Process (2계단 배양방법을 이용한 인삼 모상근의 대량배양과 Ginsenoside 생산)

  • Ko, Kyeong-Min;Yang, Deok-Chun;Park, Ji-Chang;Choi, Kang-Ju;Choi, Kwang-Tae;Hwang, Baik
    • Journal of Plant Biology
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    • v.39 no.1
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    • pp.63-69
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    • 1996
  • A hairy root clone of Panax ginseng C.A. Meyer, HRB-15 was cultured iu various conditions with 3 L bubble type bioreactor to enhance both growth and ginsenoside production. The hairy roots were more rapidly grown under the dark condition than under the light condition. However, total amount of ginsenoside of hairy roots cultured under the light for 30 days increased 2 folds as compared with the dark condition and was 1.10% based on 6 ginsenosides. Especially, ginsenoside-Re was significantly increased and some ginsenosides except for ginsenoside-Re was slightly reduced. Also, the growth of hairy roots decreased about 30% as compared with the dark condition. In contrast, addition of sodium acetate led to decreased production of ginsenoside and growth of hairy roots under light condition. The influence of potassium dihydrogenphosphate concentration was examined in MS medium and a 1.25 mM concentration was found to be the most appropriate for growth and ginsenoside production under light condition. Two-step process of hairy roots culture with yeast elicitation or without ammonia in culture medium was developed to enhance growth and giusenoside synthesis. $50\;\mu\textrm{g}$ of yeast elicitor per g of fresh weight showed a synergistic effect on the ginsenoside synthesis of hairy roots on 20 days after culture. At that time, the content of total ginsenoside was 1.15%, while the growth of hairy roots decreased 21 % as compared with the dark condition. In addition, when elimination of ammonia on 20 days after culture, the content of total ginsenoside was 1.26% with significant increment of ginsenoside-Rd (0.27%) in addition to ginsenoside-Re and the growth of hairy roots decreased 10% as compared with the dark condition. In this system, we have demonstrated a unique two-step process of hairy root cultures to maximize biomass and secondary metabolites. It has found possibility to enhance ginsenosides production by growing hairy roots in this method.

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Photosynthetic Characteristics and Cellular Tissue of Chinese Cabbage are Affected by Temperature and $CO_{2}C$ Concentration (온도와 $CO_{2}C$ 농도에 따른 배추의 광합성특성 및 세포조직의 변화)

  • Lee, Sang-Gyu;Moon, Ji-Hye;Jang, Yoon-Ah;Lee, Woo-Moon;Cho, Ill-Hwan;Kim, Seung-Yu;Ko, Kwan-Dal
    • Journal of Bio-Environment Control
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    • v.18 no.2
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    • pp.148-152
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    • 2009
  • Numerous studies have presented evidence that global atmospheric carbon dioxide ($CO_{2}$ ) concentration and temperature is increasing every year. Both of the $CO_{2}$ and temperature are important components for photosynthesis activity of plants and thusgrowth and yield. However, little information is available in terms of the reaction of vegetable plants to increased $CO_{2}$ concentration and temperature, and also the reaction to a complex condition of both increased $CO_{2}$ concentration and temperature. The aim of this research was therefore to investigate changes in growth, photosynthetic activity and ultra-cellular structure of leaf tissue of Chinese cabbage. Plants were grown under either of elevated $CO_{2}$ concentration (elevated $CO_{2}$, 2-fold higher than atmospheric $CO_{2}$ ) or elevated temperature (elevated temp, 4$^{\circ}C$ higher than atmospheric temperature), under both of elevated $CO_{2}$ concentration and elevated temperature (elevated temp+$CO_{2}$), and under atmospheric $CO_{2}$ concentration and temperature (control). The treatment of 'elevated temp' negatively affected leaf area, fresh weight, chlorophyll and starch content. However, when the treatment of 'elevated temp' was applied coincidently with the treatment of 'elevated $CO_{2}$', growth and photosynthetic performance of plants were as good as those in the treatment of 'elevated $CO_{2}$', Microscopic study resulted that the highest starch content and density of cells were observed in the leaf tissue grown at the treatment of 'elevated $CO_{2}$', whereas the lowest ones were observed in the leaf tissue grown at the treatment of 'elevated temp'. These results suggest that when Chinese cabbage grows under a high-temperature condition, supplement of $CO_{2}$ would improve the growth and yield. In our knowledge, it is the first time to determine the effect of a complex relationship between the increased $CO_{2}$ concentration and temperature on the growth of Chinese cabbage.

Effect of Nursery Period on the Growth and Yield of Green Papaya (Carica papaya) Production under Non-Heated Greenhouse (청과용 파파야 무가온 생산시 육묘기간이 생육특성 및 수량에 미치는 영향)

  • Seong, Ki-Cheol;Kim, Chun Hwan;Jeong, Yong Bin;Lim, Chan Gyu;Moon, Doo Kyong
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.212-217
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    • 2016
  • This study was conducted to investigate the effect of nursery period on growth and yield attribute of green papaya (var. Red lady). The nursery period was 3, 5, 7, 9, 11 and 13 months and the green papaya was transplanted on 15 April, 2015 in a non-heated greenhouse. The plant height, node number and fresh weight of nursery plant were increased as the nursery periods increased. The growth of green papaya with 13 months nursery period was better than those of other treatments. First harvest after transplanting was increased as the nursery periods were shorten. It took 137 days (18 August) at 13 months treatment, and 184 days (2 October) at 3 months treatment. The fruit length and diameter were smallest at 3 months treatment and there was no significant difference among other treatments. The fruit yield was also influenced by the nursery periods, the commercial yield was also increased as the nursery periods increased. The commercial yield was highest at 13 months treatment (3,172kg/10a), followed by 11 (2,247kg/10a) and 9 months treatment (2,357kg/10a). At 7 and 5 months treatment were 1,942kg/10a and 1,787kg/10a, respectively and the yield was lowest at 3 months treatment (1,443kg/10a). The commercial yield was significantly decreased under 7 months treatment. Although the harvest time of 11 months treatment was earlier than that of other treatments in non-heated greenhouse, 9 month treatment will be more recommendable for green papaya production because of operating costs.

Effect of Artificial Light Sources on the Growth of Apple Rootstock M.9 Seedling (인공광원이 사과 대목 M.9 묘 생육에 미치는 영향)

  • So, Eun Hee;An, Jin Hee;Kim, Jae Kyung;Yoon, Yeo Joong;Park, So Jeong;Na, Jong-Kuk;Choi, Ki Yong
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.341-348
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    • 2018
  • To investigate the effect of light sources on the growth and photosynthesis of the dwarf apple rootstock M.9 for the production of standard seedlings, the plants were cultivated in a controlled environment for 6 weeks. The sources of light are six treatments [Red (R), Blue (B), White (W), RBUV (R7B3 containing UV-A), RBW (R3B1W1), SMF (high pressure sodium + metal halide + fluorescent lamp) under $154{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Growth characteristics of apple seedlings varied depending on artificial light source at 3 weeks and 6 weeks. The plant height of apple seedling was high in the R, RBUV, RBW, and SMF light sources at 3 weeks, and in the R light at 6 weeks. There was no significant difference on stem diameter among the treatments at 3 weeks, but showed high in RBUV and RBW light at 6 weeks. Leaf number was the highest in RBUV light at 3 and 6 weeks. The chlorophyll content (SPAD value) was high in the B and RBUV light at 3 weeks, but it was not significant at 6 weeks. The growth rate to height of the R light (1.12mm/day) was the highest among the treatments, followed by RBUV, RBW, SMF, W and then B. Leaf area was the highest in RBUV and RBW lowest in B. Specific leaf area was high in W and fresh and dry weight were high in RBUV. The photosynthetic rate at 6 weeks was highest in the B and lowest in the R. Stomatal conductivity and transpiration rate were higher in the B and W compared to the other light sources. Therefore, we are considered that light sources for growth of dwarf apple rootstock M.9 seedlings are suitable the R, RBUV, and RBW light sources with a high mixing ratio of Red and Red +Blue.

Current Research Trend of Postharvest Technology for Chrysanthemum (국화 수확 후 관리기술의 최근 연구 동향)

  • Kim, Su-Jeong;Lee, Seung-Koo;Kim, Ki-Sun
    • Korean Journal of Plant Resources
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    • v.25 no.1
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    • pp.156-168
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    • 2012
  • Chrysanthemum is a cut flower species that normally lasts for 1 to 2 weeks, in some cases 3-4 weeks. This has been attributed to low ethylene production during senescence. Reduction in cut flower quality has been attributed to the formation of air embolisms that partially or completely blocks the water transport from the vase solution to the rest of the cut flower stem, increasing hydraulic resistance which may cause severe water stress, yellowing, wilting of leaf, and chlorophyll degradation. Standard type chrysanthemum can be harvested when buds were still tightly closed and then fully opened with the simple bud-opening solution. Standard type chrysanthemum can also be harvested when the minimum size of the inflorescence is about 5-6 cm bud which opened into the first flower full-sized flower. While spray varieties can be harvested when 2-4 most mature flowers have opened (40% opening). Cut flowers are sorted by stem length, weight, condition, and so on. Standard chrysanthemum is 80 cm length for standard type and 70cm for spray type. Pre-treatment with a STS, plant regulator such as GA, BA, 1-MCP, chrysal, germicide, and sucrose, significantly improved the vase life and quality of cut flowers. It is well established that vase solutions containing sugar can improve the vase life of cut chrysanthemum. Chrysanthemum is normally packed in standard horizontal fiberboard boxes. Chrysanthemum should normally be stored at $5{\sim}7^{\circ}C$. Precooling resulted in reduction in respiration, decomposition, and transpiration activities as well as decoloration retardation. There was significant difference between "wet" storage in 3 weeks and "dry" storage in 2 weeks. In separate pulsing solution trials, various germicides were tested, as well as PGRs to maintain the green color of leaves and turgidity. Prolonging vase life was attained with the application of optimal solution such as HQS, $AgNO_3$, GA, BA and sucrose. This also retarded senescence in leaves of cut flower stems. Fresh cut chrysanthemum can be transported using a refrigerated van with $5{\sim}7^{\circ}C$. Increasing consumption and usage of cut chrysanthemum of various cultivars would require efficient transport system, and effective information exchange among producer, wholesaler, and consumer.

Studies on the Utilization of Persimmons -(Part 6) Investigation of the Optimum Thickness of Film Bag for Polyethylene Film Storage of Fuyu- (감의 이용(利用)에 관(關)한 연구 -(제6보(第六報)) 부유시의 Polyethylene Film 저장(貯藏)에 따른 최적(最適) Film 두께의 조사(調査)-)

  • Sohn, T.H.;Choi, J.U.;Seog, H.M.;Cho, R.K.;Seo, O.S.;Kim, S.T.;Ha, Y.S.;Kang, J.H.
    • Korean Journal of Food Science and Technology
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    • v.10 no.1
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    • pp.78-82
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    • 1978
  • In a series of studies on the utilization of persimmons, the purpose of this experiment was to examine the optimum thickness of film using different number of persimmons per film bag. 'Fuyu', persimmon variety was used in this investigation. The results obtained were as follows: The optimum thickness of the film bag was 0.08mm, 0.06mm, and 0.04mm for the bags packed with 3, 10 and 50 persimnons, respectively. The changes in the ratio of firmness, loss of fresh weight, titrable acidity and percentage of sugar contents were minimal in these three optimum combinations than the others. These results could be explained by the balanced optimum gas concentration, $CO_2$ 5-10% and $O_2$ 5%, in those three optimum combination. Therefore, it was suggested that the different thickness of film bag needs a particular number of fruits packed per bag for the long term storage in persimmons.

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