• 약학회지
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• 제42권3호
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• pp.274-274
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• 1998

Aclarubicin(ACL)-entrapped freeze dried liposomes were prepared using Microfludizer to attain a sustained release at targeted organs in a prolonged time so that it can reduce th e side effect and maximize the therapeutic effect. The freeze-dried liposomes were evaluated for pharmacokinetics, antitumor activity against Sarcoma 180, cytotoxicity against L1210 and A549 tumor cells, spleen toxicity and myelosuppressive action. The AUC0->8hr values were 122+/-42, 382+/-140, 419+/-171, 835+/-206 and 443+/-309mcg min/ml for free ACL. ACL-liposome formulation I, II, III and IV, respectively. Cytotoidcity of ACL-entrapped liposomes against L1210 and A549 tumor cells was 2-4 times higher than that of free aclarubicin. ACL-liposome formulation I(PC/CHOL/TA) showed the most potent antitumor activity against Sarcoma 180 in mice. The loss of body weight was much smaller with ACL-entrapped liposomes than free ACL after I.p. injection at a dose of 2 mg/kg/day. Compared to free ACL, ACL-entrapped liposomes expressed a lower and delayed spleen toxicity up to 5th day after I.v. administration. Myelosupperssion seemed to be lower with ACL-entrapped liposome of PC/PC-hydrate/CHOL/TA (formulation III) than free aclarubicin.

• 한국식품영양과학회지
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• 제44권3호
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• pp.449-454
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• 2015

본 연구는 어린 콜라겐 500 g, 액상의 식용식물 추출물 375 g, 덱스트린 125 g을 물 1,000 mL에 녹여 1:0.75:0.25:2의 비율로 제조한 후 동결건조한 다음 분쇄하여 제조한 어린 콜라겐 동결건조 혼합분말(freeze-dried collagen mixture powder, FDCMP)을 0.5, 1.0, 1.5 및 2.0% 비율로 칼국수 반죽에 첨가하여 제조한 칼국수의 품질 특성을 분석하여 FDCMP의 첨가가 칼국수의 품질 특성에 미치는 영향을 조사하였다. 조리 후 칼국수의 중량과 부피는 FDCMP 첨가량이 많을수록 낮게 나타났고, 칼국수 삶은 물의 탁도는 유의적인 수준으로 FDCMP 첨가구가 높게 나타났다. 수분 결합 능력은 밀가루와 물보다 FDCMP와 물의 수분 결합 능력이 높게 나타났다. 색도는 조리 전후의 칼국수에서 FDCMP 첨가량이 많을수록 명도 L값은 감소하고 적색도 a값과 황색도 b값은 증가하였다. 기계적 조직감 측정은 조리한 칼국수의 경도와 씹힘성는 FDCMP 첨가량이 증가할수록 감소하였으며, 점착성, 응집성과 검성은 FDCMP 첨가량이 증가할수록 증가하였다. 탄력성과 복원성은 FDCMP 첨가 시 대조구와 유의적인 차이는 없었다. 관능검사 결과에서는 대조구보다 FDCMP 0.5% 첨가구가 조직감, 입 안에서의 느낌, 종합적인 기호도가 높게 나타났다. 따라서 칼국수 개발 및 품질 향상을 위한 어린 콜라겐 동결건조 혼합분말의 첨가는 0.5%가 가장 적당하다고 사료된다.

• Journal of Pharmaceutical Investigation
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• 제25권3호
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• pp.249-264
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• 1995

Although liposome has many advantages as a pharmaceutical dosage form, its application in the industrial field has been limited because of some problems such as preparation method, reproducibility, scale-up, stability and sterilization etc. Liposomes prepared by microemulsification method had defined size, narrow size distribution, reproducibility and high entrapment efficiency. For enhancing the stability, the dry form of liposome was recommended. These types of liposome are proliposome and freeze-dried liposome. The liposome must have some properties for preparing of freeze-dried liposome; small size $(50{\sim}200\;nm)$, narrow size distribution and cryoprotectant. In this experiment, the liposomes containing 5-Fluorouracil(5-FU) and its prodrug(pentyl-5-FU-1-acetate; PFA, hexyl-5-FU-1-acetate; HFA) were made with soybean phosphatidylcholine, cholesterol, stearylamine(SA) and dicetyl phosphate(DCP) employing hydration method or microemulsification method using $Microfluidizer^{TM}$. Both or liposome types were MLV and MEL. After preparation, freeze drying and rehydration were performed. In the process of freezing, trehalose(Tr) was added as a cryoprotectant. Their evaluation methods were as follows; entrapment efficiency, mean particle size and size distribution, dissolution test, retain of entrapment efficiency and turbidity after freeze-drying. The results are summarized as belows. The entrapment efficiency of 5-FU was dependent on total lipid concentration and cholesterol content but that of PFA and HFA was decreased when cholesterol was added. When DCP and SA were added, entrapment efficiency was decreased. As the partition coefficient of drug was increased, entrapment efficiency was increased. Under the same condition, entrapment efficiency of MEL is similar to that of MLV. The mean particle size and size distribution of MEL were smaller than those of MLV. Dissolution rates of drug from both liposome types were comparatively similar. Dissolution rates of drugs with serum and liver homogenate were faster than without these material. After preparation of liposome, free drug was removed efficiency by Dowex 50W-X4. When liposome was freeze-dried and then rehydrated in the presence of Tr, characteristics of liposome were maintained well in MEL than MLV. Tr Was used successfully as a cryoprotectant in the process of freeze drying and the optimal ratio of Tr:Lipid was 4:1(g/g).

• Korea-Australia Rheology Journal
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• 제13권1호
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• pp.1-12
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• 2001

This paper describes the application of our recently developed two-phase model for flow-induced crystallization (FIC) to the simulation of fiber spinning and film blowing. 1-D and 2-D simulations of fiber spinning include the combined effects of (FIC), viscoelasticity, filament cooling, air drag, inertia, surface tension and gravity and the process dynamics are modeled from the spinneret to the take-up roll device (below the freeze point). 1-D model fits and predictions are in very good quantitative agreement with high- and low-speed spinline data for both nylon and PET systems. Necking and the associated extensional softening are also predicted. Consistent with experimental observations, the 2-D model also predicts a skin-core structure at low and intermediate spin speeds, with the stress, chain extension and crystallinity being highest at the surface. Film blowing is simulated using a "quasi-cylindrical" approximation for the momentum equations, and simulations include the combined effects of flow-induced crystallization, viscoelasticity, and bubble cooling. The effects of inflation pressure, melt extrusion temperature and take-up ratio on the bubble shape are predicted to be in agreement with experimental observations, and the location of the frost line is predicted naturally as a consequence of flow-induced crystallization. An important feature of our FIC model is the ability to predict stresses at the freeze point in fiber spinning and the frost line in film blowing, both of which are related to the physical and mechanical properties of the final product.l product.

• 터널과지하공간
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• 제21권2호
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• pp.145-150
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• 2011

사암과 안산암에 대한 풍화특성을 살펴보기 위하여 동결융해 반복시험을 실시한 후, 두 암석에 대한 중량 감소율, 탄성파속도, 일축압축강도 등의 물성 변화를 측정하였다. 중량 변화의 경우 두 암석 모두에서 동결 융해 반복 횟수가 증가함에 중량이 감소하는 경향을 보였다. 특히 안산암에서는 중량 감소 경향이 시험편에 따라 매우 불규칙하게 나타났다. 탄성파속도 변화에 있어서 사암은 5%이상의 감소 경향을 보였으며, 안산암은 동결융해 반복시험 500 사이클까지 탄성파속도 변화가 거의 없다가 1000 사이클부터 5%이상의 감소 현상을 보였다. 이것은 안산암이 비교적 풍화에 강한 암석인 반면, 사암은 풍화받기 쉬운 암석인 것을 의미한다. 또한 안산암의 탄성파속도 변화 양상은 안산암의 중량 변화와 일치한다. 사암의 일축압축시험 결과에서 일축압축강도는 동결융해 반복시험 초기 사이클 구간에서 미미한 강도저하를 보이다 64 사이클부터는 불규칙한 경향을 나타냈다. 결과적으로, 중량 감소가 적은 암석 시험편일수록 강도저하율도 작게 나타났다.

• 한국지반신소재학회논문집
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• 제8권1호
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• pp.53-59
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• 2009

본 연구에서는 변형된 동결-융해 시험장치를 이용하여 동상민감성흙의 동결-융해 과정시 지반공학적 특성을 평가하였다. 흙시료의 정상상태에서 동상이 발생되는 양은 전체 동상량의 70~80% 정도로 정상상태의 동상이 일어나기 위한 열흐름 조건을 제안하였다. 즉, 동결시 동결전선에서 순열흐름을 0으로 하기 위해서 열적 경계조건을 변화시키는 방법이다. 제안된 방법으로 변형된 동결-융해 시험의 결과에 대하여 적합성을 평가하였다. 또한, 동상 발현중 제안된 방법을 적용 결과, 장기적인 동상현상을 제시하였으나, 장기적 동상은 매우 작은 동상량이 나타나기 때문에 공학적 요소에서는 무시할 수 있는 동상으로 판단하였다.

• 한국세라믹학회지
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• 제33권9호
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• pp.1057-1063
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• 1996

Y2O3-CeO2-ZrO2 powders were prepared from water-soluble salts using a coprecipitation method. The forming process of oxide and the characteristics of the calcined powders treated in different drying conditions were investigated. The oxidation was occurred at the temperature of around 40$0^{\circ}C$ and the main crystallization of ZrO2 around $600^{\circ}C$. On calcination at $600^{\circ}C$ heating lamp-dried powders consisted of agglomerates of globular morphology with average agglomerate size of 2.27${\mu}{\textrm}{m}$ and specific surface area of 68.3m2/g and spray dried powders contained dense spheric particles with average agglomerate size of 1.35${\mu}{\textrm}{m}$ and specific surface area of 11.0m2/g which exhibited low agglomeration tendency. Removal of the water by a freeze-drying technique produced calcined powders containing flake-like secondary particle structures with wide agglomerate size distri-bution of 0.1-60${\mu}{\textrm}{m}$ and specific surface area of 24.5${\mu}{\textrm}{m}$. The 20 MPa-pressed density (36.8-41.4% T,D) of calcined powders did not nealy depend on drying methods whilst compaction ratio of calcined powders derived from freeze-drying was the highest ( 6.24) among three drying methods. On continuous heating up to 150$0^{\circ}C$ the sinterability of calcined powders derived from heating lamp-drying was superior to those derived from spray-and freeze-drying. The final sintered density of calcined powders was the highest (96% T,D at 150$0^{\circ}C$) in case of heating lamp-drying.

• 한국식품저장유통학회지
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• 제6권1호
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• pp.87-91
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• 1999

In an attempt to test experimental condition of preparing grape powder, grapes having less commercial value was used and tried. With drying method, spray and freeze drying were satisfactory to produce power. Moisture content and odor retention were better by the latter method. Three grape strains stored for 40 days contained more odors than those stored for 5 days. Maltose 90% plus dextrin 10% was suitable for drying support. To increase odror sense, citric acid and vitamin C can be added up to 0.1 and 0.2%, respectively. Considering these conditions, grape complex powder prapared from grape powder 20% comprising drying support, glucose 79.7%, citric acid 0.1%, vitamin C 0.2% with freeze drying was the best by overall evaluation including sensory test. When campbell and neomuscut were mixed by 15:5 or 10:10, sensory evaluation was also ameliorated.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.384-387
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• 2004

The purpose of the present investigation was to develop a novel method for cell immobilization. Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated- immobilized cells. The beads were then placed at $-15^{\circ}C$ for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads due to water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system. This study showed that bioreactor performance can be improved up to 2 times by the use of the dehydrated beads.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2004년도 추계 학술발표회 제16권2호
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• pp.117-120
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• 2004

Up to now, many researches have been performed md verified that many properties of concrete can be improved by using mineral admixtures such as blast furnace slag, silica fume, and expansive admixture. But it is not clear whether there is any need for entraining air to make a high strength concrete using expansive admixture and mineral admixtures to insure enough freeze-thaw resistance. this paper presents the strength and durability properties of high strength concrete using expasive admixtures and industrial by-products. It was observed from the test results that very high strength concrete$(W/B=20\%)$ is not needed to be air entrained and high strength concrete$(W/B=30\%)$ using expansive admixture and mineral admixtures is needed to be entrained $2\~4\%$ air.