Production of Oligosaccharides from Sucrose for Animal Industry

  • Lee, Jae-Heung (Dept. of Food Bio-Technology, Sungkyunkwan University) ;
  • Shin, Hyung-Tai (Dept. of Food Bio-Technology, Sungkyunkwan University) ;
  • Lee, Soo-Won (Dept. of Food Bio-Technology, Sungkyunkwan University)
  • Published : 2004.05.28

Abstract

The purpose of the present investigation was to develop a novel method for cell immobilization. Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated- immobilized cells. The beads were then placed at $-15^{\circ}C$ for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads due to water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system. This study showed that bioreactor performance can be improved up to 2 times by the use of the dehydrated beads.

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