• KSBB Journal
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• 제4권3호
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• pp.288-292
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• 1989

인참 근 조직에 Agrobacterium rhizogenes strain $A_4$를 접종하여 hairy roots 유도와 유도된 hairy roots의 배양 조건을 조사하였다. 48시간 배양된 균($2{\times}A92$bacteria/ml)을 접종하여 암조건($26{pm}1^{\circ}C$)하에서 배양하였을때 6-7주후 tumor가 형성되었으며, 10-12주후 hairy roots가 유도되었다. 근 년별 hairy roots 유도율은 5년근이 4, 6년근에 비하여 높았으며, 배지에 IAA, 2, 4-D, IBA 및 tryptophan 을 각각 첨가시켰을때 15-30mg/l tryptophan에서 tumor 및 hairy roots 유도율이 증가되었음을 나타내었다. 또한 유도된 hairy roots hormone-free인 RCM배지(sucrose 3%, pH 4.5)에서 배양하였다.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.673-679
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• 2004

A microbial cryoprotectant formulation using a W/O/W multiple emulsion system was developed. The psychrotolerant microorganism, B4, isolated from soil in South Korea, was observed by the drop freezing method, in which the microorganism sample inhibited ice nucleation activity. The antifreeze activity was eliminated when the microorganism sample was treated with protease, indicating that the antifreeze activity was due to the presence of antifreeze protein. The result of the l6S rDNA sequencing indicated the B4 strain was most closely related to a species of the genus Bacillus. Culture broth of B4 strain (Bacillus sp.) and rapeseed oil containing 1 % polyglycerine polyricinolate (PGPR) were used as core and wall material, respectively. The most stable W/O emulsion was prepared at a core/oil ratio of 1:2. The highest W/O/W emulsion stability was achieved when the primary emulsion to external aqueous phase containing 0.5% caster oil polyoxyethylene ether $(COG25^{TM})$ ratio was 1:1. Microcrystalline cellulose showed better W/O/W emulsion stability than other polymer types. The viability of cells in a W/O/W emulsion was higher than free cells during storage at $37^\circ{C}$. An acidic pH and UV exposure decreased the viability of free cells, but cells in W/O/W emulsion were more stable under these conditions.

• 한국염색가공학회지
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• 제10권3호
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• pp.10-19
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• 1998

This paper investigates the change of mechanical properties and thermal shrinkage in commercial multi-filament PET(polyethylene terephthalate) , namely, regular yarn, POY, DTY and composite yarn. To determine changing the effects of processing steps, these were examined at three steps process simulation conditions. The first step is sizing simulation$(S-1\;step\;:\;130^\circ{C}\times2\;min$., hot air treatment under 0.1 gf/d load), the second step is scouring simulation$(S-2\;step\;:\;100^\circ{C}\times20\;min$., boiling water treatment under free tension)and final step is setting simulation$(S-3\;step\;:\;180^\circ{C}\times2\;min$., hot air treatment under free tension). Regular yarn in multi-step treatment showed higher shrinkage at S-3 step and DTY showed higher in at S-1 step. While POY was relaxed at S-1 step, composite yarn showed different shrinkage properties depending on composite yarn type. Mechanical properties showed good relationship with shrinkage : high shrinkage makes initial modulus decrease and bleating strain increase. It also makes decreasing yield strain and yield stress decrease.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2005년도 춘계학술대회 논문집
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• pp.253-257
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• 2005

A tube hydroformability testing system was designed and fabricated so as to observe the forming process and to apply forming condition along arbitrarily pre-programmed internal pressure-axial feed path. The forming limit diagram of A6063 extruded tube, of 40.6 mm outer diameter and 2.25 mm thickness, was successfully obtained through free bulging and T-forming tests except the region of high positive minor strain. It is found that the data points marked on the FLD are mostly located near the strain paths from the finite element analysis excluding the cases of large axial feed. There exist data points even in the area beyond the uniaxial tension mode, since the reduction in thickness decreases due to the axial feed. The forming limit from T-forming test was considerably lower than that from free bulge test. It seems because the deformation is localized at the pole.

• Advances in nano research
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• 제5권4호
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• pp.303-311
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• 2017

The continuum elasticity model is applied to investigate quantitatively the growth features and nucleation mechanism of quantum dots, nanopits, and joint QDs-nanopits structures in GaInAlN quasyternary systems. We have shown that for GaInAlN material system at the critical strain of ${\varepsilon}^*=0.039$ the sign of critical energy and volume is changed. We assume that at ${\varepsilon}={\varepsilon}^*$ the mechanism of the nucleation is changed from the growth of quantum dots to the nucleation of nanopits. Obviously, at small misfit (${\varepsilon}$ < ${\varepsilon}^*$), the bulk nucleation mechanism dominates. However, at ${\varepsilon}$ > ${\varepsilon}^*$, when the energy barrier becomes negative as well as a larger misfit provides a low-barrier path for the formation of dislocations, the nucleation of pits becomes energetically preferable. The free energy of mixing for $Ga_{1-x-y}In_xAl_yN$ quasiternary system was calculated and studied and its 3D sketch was plotted.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.64-71
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• 2022

The discarding of wastes into the environment is a significant problem for many communities. Still, food waste can be used for lactic acid bacteria (LAB) growth. Here, we evaluated three growth media equivalent to de Mann Rogosa Sharpe (MRS), using apple bagasse, yeast waste, fish flour, forage oats, and cheese whey. Cell-free supernatants of eight LAB strains were tested for antimicrobial activity against nine indicator microorganisms. The supernatants were also evaluated for protein content, reducing sugars, pH, and lactic acid concentration. Cell-free supernatants from fish flour broth (FFB) LAB growth were the most effective. The strain Leuconostoc mesenteroides PIM5 presented the best activity in all media. L. mesenteroides CAL14 completely inhibited L. monocytogenes and strongly inhibited Bacillus cereus (91.1%). The strain L. mesenteroides PIM5 consumed more proteins (77.42%) and reducing sugars (56.08%) in FFB than in MRS broth (51.78% and 30.58%, respectively). Culture media formulated with agroindustrial wastes positively improved the antimicrobial activity of selected LAB, probably due to the production of antimicrobial peptides or bacteriocins.

• Advances in nano research
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• 제11권6호
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• pp.621-640
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• 2021

Effect of thickness stretching on mechanical behavior of functionally graded (FG) carbon nanotubes reinforced composite (CNTRC) laminated nanoplates resting on elastic foundation is analyzed in this paper using a novel quasi 3D higher-order shear deformation theory. The key feature of this theoretical formulation is that, in addition to considering the thickness stretching effect, the number of unknowns of the displacement field is reduced to four, and which is more than five in the other models. Single-walled carbon nanotubes (SWCNTs) are the reinforced elements and are distributed with four power-law functions which are, uniform distribution, V-distribution, O-distribution and X-distribution. To cover various boundary conditions, an analytical solution is developed based on Galerkin method to solve the governing equilibrium equations by considering the nonlocal strain gradient theory. A modified two-dimensional variable Winkler elastic foundation is proposed in this study for the first time. A parametric study is executed to determine the influence of the reinforcement patterns, power-law index, nonlocal parameter, length scale parameter, thickness and aspect ratios, elastic foundation, thermal environments, and various boundary conditions on stresses, displacements, buckling loads and frequencies of the CNTRC laminated nanoplate.

• Advances in nano research
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• 제12권2호
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• pp.117-137
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• 2022

Effect of thickness stretching on free vibration, bending and buckling behavior of carbon nanotubes reinforced composite (CNTRC) laminated nanoplates rested on new variable elastic foundation is investigated in this paper using a developed four-unknown quasi-3D higher-order shear deformation theory (HSDT). The key feature of this theoretical formulation is that, in addition to considering the thickness stretching effect, the number of unknowns of the displacement field is reduced to four, and which is more than five in the other models. Two new forms of CNTs reinforcement distribution are proposed and analyzed based on cosine functions. By considering the higher-order nonlocal strain gradient theory, microstructure and length scale influences are included. Variational method is developed to derive the governing equation and Galerkin method is employed to derive an analytical solution of governing equilibrium equations. Two-dimensional variable Winkler elastic foundation is suggested in this study for the first time. A parametric study is executed to determine the impact of the reinforcement patterns, nonlocal parameter, length scale parameter, side-t-thickness ratio and aspect ratio, elastic foundation and various boundary conditions on bending, buckling and free vibration responses of the CNTRC plate.

• Advances in nano research
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• 제12권1호
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• pp.101-115
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• 2022

Some researchers pointed out that the nonlocal cantilever models do not predict the dynamic softening behavior for nanostructures (including nanobeams) with clamped-free (CF) ends. In contrast, some indicate that the nonlocal cantilever models can capture the stiffness softening characteristics. There are substantial differences on this issue between them. The vibration analysis of porosity-dependent functionally graded nanoscale tubes with variable boundary conditions is investigated in this study. Using a modified power-law model, the tube's porosity-dependent material coefficients are graded in the radial direction. The theory of nonlocal strain gradients is used. Hamilton's principle is used to derive the size-dependent governing equations for simply-supported (S), clamped (C) and clamped-simply supported (CS). Following the solution of these equations by the extended differential quadrature technique, the effect of various factors on vibration issues was investigated further. It can be shown that these factors have a considerable effect on the vibration characteristics. It also can be found that our numerical results can capture the unexpected softening phenomena for cantilever tubes.

• Advances in materials Research
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• 제12권2호
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• pp.161-177
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• 2023

This paper proposes an analytical method to investigate the free vibration behaviour of new functionally graded (FG) carbon nanotubes reinforced composite beams based on a higher-order shear deformation theory. Cosine functions represent the material gradation and material properties via the thickness. The kinematic relations of the beam are proposed according to trigonometric functions. The equilibrium equations are obtained using the virtual work principle and solved using Navier's method. A comparative evaluation of results against predictions from literature demonstrates the accuracy of the proposed analytical model. Moreover, a detailed parametric analysis checks for the sensitivity of the vibration response of FG nanobeams to nonlocal length scale, strain gradient microstructure-scale, material distribution and geometry.