• Journal of Korean Society of Coastal and Ocean Engineers
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• v.7 no.2
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• pp.156-162
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• 1995

A numerical method for a 2-D oil boom model considering the flexibility of skirt has been developed The neater is assumed rigid and the skirt is tensioned membrane having a point mass at its end The fluid motion is potential. The kinematic condition which demands the continuity of the displacement is imposed at the joint between the floater and the skirt. The dynamic condition for the point mass is imposed at the bottom end of the skirt. The numerical method is based on the Green's function method in the frame of linear potential theory. It finds it's solution simultaneously from the total system of three equations, integral equation, the equation of motion of the floater and the equilibrium equation of the deformation of the skirt. Integral equation is derived by applying the Green's theorem to radiation potential and Green's function. Proper descretization of those three equations leads to the system of a linear algebraic equation. Due to the flexibility of skirt the motion of floater can be diminished in some range of wave frequency and furthermore the mechanism of resonance of the oil boom can be changed. The motion responses of various oil booms have been compared varying the length of the skirt and the point mass. The numerical method has been validated indirectly from the good correspondence between the motion responses of the flexible skirt model and the rigid skirt model in low frequency limit.

• Journal of Bio-Environment Control
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• v.10 no.3
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• pp.148-154
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• 2001

The uplift capacity of a pile for improving the wind resistance of the 1-2 W type plastic film pipe on greenhouses was tested using the plane and corrugated piles with various shapes and diameters. First, the resistant uplift capacity was measured by using the uplift loading on plane piles. As the uplift loading on plane piles increased, the resistant uplift capacity also increased until the loading was reached to ultimate uplift capacity. After ultimate uplift capacity was appeared the uplift displacement, the uplift capacity was decreased gradually. Secondly, the resistant uplift capacity was measured by using the uplift loading on corrugated piles. After the uplift capacity was reached the uplift displacement, the uplift capacity was continually increased or decreased. In general, the ultimate uplift capacity was independent of pile shapes, pile diameter length, and embedded pipe depth. However, the ultimate uplift capacity of a corrugated pile was twice more than that of a plane pile without regard to its diameter and embedded depth. The ultimate uplift capacity per unit pile area was increasing in deeper embedded depth. However, the longer a pile diameter was, the less ultimate uplift capacity. The uplift capacity of a plane pile, used in conjunction with the design wind velocity (26.9m.s$^{-1}$ ) of the project area, was unsatisfiable without regard to diameters and embedded depths of piles, while most of corrugated piles were well appeared uplift capacity under various experimental conditions.

• Journal of Aquaculture
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• v.20 no.1
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• pp.65-72
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• 2007

Full length complementary DNA encoding apolipoprotein A-I (apoA-I) was isolated and characterized in mud loach (Misgurnus mizolepis). Mud loach apoA-I cDNA encoding 24 bp of 5'-untranslated region (UTR), 762 bp of single open reading frame (ORF) consists of 254 amino acids and 293 bp of 3'-UTR excluding stop codon and poly (A+) tail. Two overlapping polyadenylation signals (AATAAAATAAA) was found 9 bp prior to the poly (A+) tail. Mud loach apoA-I represented considerable homology to those from other teleost species at amino acid level with conserving common features of vertebrate apoA-I. Molecular phylogenetic analysis inferred the phylogenetic hypothesis that was generally in accordance with the previous taxonomic relationship. Apolipoprotein A-I mRNA was detected in various tissues, but the mRNA levels were quite varied depending on tissues based on semi-quantitative RT-PCR. Liver and brain showed the significantly higher levels of apoA-I transcripts than other tissues. mRNA expression of apoA-I was quite low in very early stage of embryonic development, however dramatically enhanced from 8 hours post fertilization. This increased mRNA level was retained consistently up to 14 days post hatching.

• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.390-399
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• 2012

Endoglucanase gene egIV was cloned from Trichoderma viride AS 3.3711, an important cellulose-producing fungus, by using an RT-PCR protocol. The egIV cDNA is 1,297 bp in length and contains a 1,035 bp open reading frame encoding a 344 amino acid protein with an estimated molecular mass of 35.5 kDa and isoelectronic point (pI) of 5.29. The expression of gene egIV in T. viride AS 3.3711 could be induced by sucrose, corn straw, carboxymethylcellulose (CMC), or microcrystalline cellulose, but especially by CMC. The transcripts of egIV were regulated under these substrates, but the expression level of the egIV gene could be inhibited by glucose and fructose. Three recombinant vectors, pYES2-xegIV, $pYES2M{\alpha}$-egIV, and $pYES2M{\alpha}$-xegIV, were constructed to express the egIV gene in Saccharomyces cerevisiae H158. The CMCase activity of yeast transformants $IpYES2M{\alpha}$-xegIV was higher than that of transformant IpYES2-xegIV or $IpYES2M{\alpha}$-egIV, with the highest activity of 0.13 U/ml at induction for 48 h, illustrating that the modified egIV gene could enhance CMCase activity and that $MF{\alpha}$ signal peptide from S. cerevisiae could regulate exogenous gene expression more effectively in S. cerevisiae. The recombinant EGIV enzyme was stable at pH 3.5 to 7.5 and temperature of $35^{\circ}C$ to $65^{\circ}C$. The optimal reaction condition for EGIV enzyme activity was at the temperature of $55^{\circ}C$, pH of 5.0, 0.75 mM $Ba^{2+}$, and using CMC as substrate. Under these conditions, the highest activity of EGIV enzyme in transformant $IpYES2M{\alpha}$-xegIV was 0.18 U/ml. These properties would provide technical parameters for utilizing cellulose in industrial bioethanol production.

• Magazine of the Korea Concrete Institute
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• v.4 no.1
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• pp.135-145
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• 1992

With the increasing tendency to construct high rise reinforced concrete building~i, it is required to use high strength materIals, smaller member sections, and larger reinforcing bars, I t is generally recognized that under severe seismic loads beam column jomts may become more critical structural components than other structural elements. In a ductile momentresistmg reinforced concrete frame, the connection of bearncolumn must be capable of resistll1g the large lateral forces caused by seismic actions, The purpose of this experimental study is to evaluate and ll1vestigate the earthquake resistant perform ance of beam-colurrm subassemblies constructed with high-strength concrete cast by the concrete of com¬pressive strength of 700kg / cm2 subjected to reversed cyclic loadings. New approaches for moving the plastic hinging zone away from the column face and preventing the di¬agonal crack in the joint region are adopted to advance the earthquake-resistant performance of beam-column subassemblies using high-strengh concrete under severe earthquake-type loading. Exper¬imental results indicate that the modified new details which are introduced by intermediate reinforcement in the beam over a specific beam length adjacent to the joint are able to attain the stable hysteretic behavior and the enhancement of earthquake-resistant performance. Keywords: high strength concrete: beam-column Joints; seirnic loads(reversed cyclic loading) : earth¬quake-resistant performance; plastic hinge zone: diagonal crack: intermediate reinforce¬ment ; closed strirrup: hysteretic behavior: enhancement .

• Journal of the Korea Concrete Institute
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• v.24 no.3
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• pp.259-266
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• 2012

A general earthquake resistant design philosophy of ductile frame buildings allows beams to form plastic hinges adjacent to beam-column connections. In order to carry out this design philosophy, the ultimate bond or shear strength of the beam should be greater than the flexural yielding force and should not degrade before reaching its required ductility. The behavior of RC members dominated by bond or shear action reveals a dramatic reduction of energy dissipation in the hysteretic response due to the severe pinching effects. In this study, a method was proposed to predict the deformability of reinforced concrete members with short-span-to-depth-ratios, which would result in bond failure after flexural yielding. Repeated or cyclic loading produces a progressive deterioration of bond that may lead to failure at lower cyclic bond stress levels. Accumulation of bond damage is caused by the propagation of micro-cracks and progressive crushing of concrete in front of the lugs. The proposed method takes into account bond deterioration due to the degradation of concrete in the post yield range. In order to verify bond deformability of the proposed method, the predicted results were compared with the experimental results of RC members reported in the technical literature. Comparisons between the observed and calculated bond deformability of the tested RC members showed reasonably good agreement.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.9 no.4
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• pp.427-438
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• 1998

The bit error rate(BER) performance of Trellis Coded QPSK signal in the presence of cochannel interference (CCI) and Rician fading is investigated. The trellis coded QPSK system adopts Maximum Ratio Combining (MRC) diversity reception and Reed-Solomon code to enhance system performance. Using the derived error probability equation, the error performance of trellis coded QPSK system has been evaluated and shown in figures to discuss as a function of signal power to noise power ratio (SNR), signal power to interference power ratio(SIR), direct to indirect signal power ratio ($K_R$), the number of diversity branch (M), the frame length of Reed-Solomon code (n), the number of error correction symbol (t), and the number of state of trellis encoder. From the results, we know that proposed system is affected by cochannel interference and fading in microcell environment. Also, BER performance of Trellis Coded QPSK system can be improved as increasing either the power of desired signal or the value of SIR. And the BER floor in microcellular system is caused by the cochannel interference and it occurs at high BER when SIR is low. And Reed-Solomon code (n=15, t=2) is more effective to restrain the affection of CCI and fading than MRC diversity reception (M=2).

• Journal of Korean Association for Spatial Structures
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• v.8 no.5
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• pp.47-58
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• 2008

Spatial structure is an appropriate shape that resists external force only with in-plane force by reducing the influence of bending moment, and it maximizes the effectiveness of structural system. With this character of the spatial structure, generally long span is used. As a result, large deflection is accompanied from the general frame. the structure is apt to result in a large deflection even though this structure experiences a small displacement in absence. Usually, nonlinear analysis in numerical analysis means geometric nonlinearity and material nonlinearity and complex nonlinearity analysis considers both of them. In this study, nonlinear equation of equilibrium considering geometric nonlinearity as per finite element method was applied and also considered the material nonlinearity using the relation of stress-strain in element. It is applied to find unstable result for tracing load-deflection curve in the numerical analysis tech. especially Arc-length method, and result of the analysis was studied by ABAQUS a general purpose of the finite element program. It is found that the present analysis predicts accurate nonlinear behavior of plane and space truss.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.38 no.3
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• pp.304-315
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• 2001

In this paper, we peformed the real-time implementation of AMR(Adaptive Multi-Rate) speech coding algorithm which is adopted for IMT-2000 service using TMS320C6201, i.e., a Texas Instrument´s fixed-point DSP. With the ANSI C source code released from ETSI, optimization is performed to make it run in real-time with memory as small as possible using the C compiler and assembly language. Implemented AMR speech codec has the size of 32.06 kWords program memory, 9.75 kWords data RAM memory, and 19.89 kWords data ROM memory. And, The time required for processing one frame of 20 ms length speech data is about 4.38 ms, and it is short enough for real-time operation. It is verified that the decoded result of the implemented speech codec on the DSP is identical with the PC simulation result using ANSI C code for test sequences. Also, actual sound input/output test using microphone and speaker demonstrates its proper real-time operation without distortions or delays.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.2
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• pp.71-84
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• 2014

The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.