Browse > Article

Characterization of Mud Loach (Misgurnus mizolepis) Apolipoprotein A-I: cDNA Cloning, Molecular Phylogeny and Expression Analysis  

Lee, Youn-Ho (National Fisheries Research and Development Institute)
Noh, Jae-Koo (National Fisheries Research and Development Institute)
Kim, Keun-Yong (Department of Aquaculture, Pukyong National University)
Cho, Young-Sun (Department of Aquaculture, Pukyong National University)
Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
Kim, Dong-Soo (Department of Aquaculture, Pukyong National University)
Publication Information
Journal of Aquaculture / v.20, no.1, 2007 , pp. 65-72 More about this Journal
Abstract
Full length complementary DNA encoding apolipoprotein A-I (apoA-I) was isolated and characterized in mud loach (Misgurnus mizolepis). Mud loach apoA-I cDNA encoding 24 bp of 5'-untranslated region (UTR), 762 bp of single open reading frame (ORF) consists of 254 amino acids and 293 bp of 3'-UTR excluding stop codon and poly (A+) tail. Two overlapping polyadenylation signals (AATAAAATAAA) was found 9 bp prior to the poly (A+) tail. Mud loach apoA-I represented considerable homology to those from other teleost species at amino acid level with conserving common features of vertebrate apoA-I. Molecular phylogenetic analysis inferred the phylogenetic hypothesis that was generally in accordance with the previous taxonomic relationship. Apolipoprotein A-I mRNA was detected in various tissues, but the mRNA levels were quite varied depending on tissues based on semi-quantitative RT-PCR. Liver and brain showed the significantly higher levels of apoA-I transcripts than other tissues. mRNA expression of apoA-I was quite low in very early stage of embryonic development, however dramatically enhanced from 8 hours post fertilization. This increased mRNA level was retained consistently up to 14 days post hatching.
Keywords
Mud loach; Apolipoprotein A-I; Phylogeny; Gene expression;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chenna, R., H. Sugawara, T. Koike, R. Lopez, T. J. Gibson, D. G. Higgins and J. D. Thompson, 2003. Multiple sequence alignment with the clustal series of programs. Nucleic Acids Res., 31, 3497-3500   DOI   ScienceOn
2 Christoffels, A., E. G. L. Koh, J.-M. Chia, S. Brenner, S. Aparicio and B. Venkatesh, 2004. Fugu genome analysis provides evidence for a whole-genome duplication early during the evolution of ray-finned fishes. Mol. Biol. Evol., 21, 1146-1151   DOI   ScienceOn
3 Kondo, H., K. Morinaga, R. Misaki, M. Nakaya and S. Watabe, 2005. Characterization of the pufferfish Takifugu rubripes apolipoprotein multigene family. Gene, 346, 257-266   DOI   ScienceOn
4 Frank, P. G and Y. L. Marcel, 2000. Apolipoprotein A-I: structurefunction relationships. J. Lipid Res., 41, 853-872
5 Jaillon, O., J. M. Aury, F. Brunet, J. L. Petit, N. Stange-Thomann, E. Mauceli, L. Bouneau, C. Fischer, et al., 2004. Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. Nature, 431, 946-957   DOI   ScienceOn
6 Li, W.-H., M. Tanimura, C.-C. Luo, S. Datta and L. Chan, 1988. The apolipoprotein multigene family: biosynthesis, structure, structure-function relationships, and evolution. J. Lipid Res., 29, 245-271
7 Hall, T. A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser., 41, 95-98
8 Wallaert, C. and P. J. Babin, 1994. Age-related, sex-related, and seasonal changes of plasma lipoprotein concentrations in trout. J. Lipid Res., 35, 1619-1633
9 Llewellyn, L., V. P. Ramsurn, T. Wigham, G. E. Sweeney and D. M. Power, 1998. Cloning, characterization and expression of the apolipoprotein A-I gene in the sea bream (Sparus aurata). Biochim. Biophys. Acta, 1442, 399-404   DOI   ScienceOn
10 Boguski, M. S., N. Elshourbagy, J. M. Taylor and J. I. Gordon, 1985. Comparative analysis of repeated sequences in rat apolipoproteins A-I, A-IV, and E. Proc. Natl. Acad. Sci. USA, 82, 992-996
11 Khuseyinova, N. and W. Koenig, 2006. Apolipoprotein A-I and risk for cardiovascular diseases. Curr. Atheroscler Rep., 8, 365-373   DOI
12 Kumar, S., K. Tamura and M. Nei, 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform., 5, 150-163   DOI   ScienceOn
13 Babin, P., C. Thisse, M. Durliat, M. Andre, M.-A. Akimenko and B. Thisse, 1997. Both apolipoprotein E and A-I genes are present in a nonmammalian vertebreate and are highly expressed during embryonic development. Proc. Natl. Acad. Sci. USA, 94, 8622-8627
14 Kim, D. S., J. Y. Jo and T. Y. Lee, 1994. Induction of triploidy in mud loach (Misgurnus mizolepis) and its effect on gonadal development and growth. Aquaculture, 120, 263-270   DOI   ScienceOn
15 Delcuve, G. P., J. M. Sun and J. R. Davie, 1992. Expression of rainbow trout apolipoprotein A-I genes in liver and hepatocellular carcinoma. J. Lipid Res., 33, 251-262
16 Babin, P. J., 1987. Plasma lipoprotein and apolipoprotein distribution as a function of density in the rainbow trout (Salmo gairdneri). Biochem. J., 246, 425-429   DOI
17 Kondo, H., I. Kawazoe, M. Nakaya, K. Kikuchi, K. Aida and S. Watabe, 2001. The novel sequences of major plasma apolipoproteins in the eel Anguilla japonica. Biochim. Biophys. Acta, 1531, 132-142   DOI   ScienceOn
18 Nam, Y. K., 2005. Tailoring fish genome and transgenic manipulation as exemplified by mud loach (Misgurnus mizolepis). Fish Genet. Breed. Sci., 35, 113-121
19 Chen, W.Y., J.A.C. John, C.-H. Lin, H.-F. Lin, S.-C. Wu, C.-H. Lin, C.-Y. Chang, 2004. Expression of metallothionein gene during embryonic and early larval development in zebrafish. Aquat. Toxicol., 69, 215-227   DOI   ScienceOn