• Natural Product Sciences
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• v.28 no.3
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• pp.153-160
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• 2022

Neuroinflammation is known to be associated with brain injury in Alzheimer's disease (AD), and the inhibition of microglial activation, a key player in inflammatory response, is considerd as important target for AD. In this study, the ethanol extract of aerial parts of Forsythia velutina Nakai, a Korean native species, significantly inhibited nitric oxide (NO) production in LPS-stimulated BV2 microglial cells. Thus, the active principles in F. velutina aerial parts were isolated based on activity-guided isolation method. As a result, six compounds were isolated and their structures were elucidated based on NMR data and the comparison with the relevant references as arctigenin (1), matairesinol (2), rengyolone (3), ursolic acid (4), secoisolariciresinol (5), and arctiin (6). Among them, four compounds including arctigenin (1), matairesinol (2), secoisolariciresinol (5), and arctiin (6) significantly inhibited NO production in a dose-dependent manner. In particular, matairesinol (2) and secoisolariciresinol (5) reduced 60% of NO production compared to LPS-treated group. This inhibitory effects of matairesinol (2) and secoisolariciresinol (5) were accompanied with the reduced expression levels of iNOS and COX-2. These results suggest that the extract of F. velutina and its active compounds could be beneficial for neuroinflammatory diseases including AD.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.136-142
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• 2013

Optimal condition for pollen germination was suggested as a basic research of flowering physiology in order to identify the characteristics of flowering and seed setting of rare and endangered Forsythia saxatilis Nakai. Pollen samples were collected during flowering time from the end of March to the beginning of April. First, a suitable germination temperature, medium sucrose concentration and germination time were determined for pollen germination and pollen tube elongation in vitro, and then an optimal pH of culture medium. Pollen germination and tube elongation were significantly different among the levels of germination temperature, sucrose concentration and germination time. Interactive effects were observed between germination temperature and time, germination temperature and sucrose concentration, germination time and sucrose concentration. Pollen germination was the highest at $10^{\circ}C$ and increased with the increase of sucrose concentration, whereas it had no relation with germination time. In addition, pollen germination and tube elongation did not increase at more than 15% of sucrose concentration and 24 hours later. Pollen germination was the highest at pH 5 (20.8%) and the lowest at pH 6 (3.8%). In conclusion, $10^{\circ}C$, 15% sucrose and pH 5 were proposed as the optimal condition for pollen germination 24 hours later of pollen culture.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.61-68
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• 2005

Objectives : This is the study of the application as the ingredients of cosmetics through the examination of the function for physiological activity of Forsythia koreana Nakai. Methods : Forsythia koreana Nakai, which had been extracted, concentrated, and freeze drying with water and ethanol, have been used for the experiment. The effects on electronic donating ability, SOD-like activity, xanthine oxidase inhibition, whitening effect, nitric oxide inhibition have been investigated in the physiological activity measurement of function experiment. Results : According to the physiological activity measurement of function experiment, it had been found that the electron donating ability, at over 100ppm of water extract and ethanol extract showed relatively high donating ability by more than 80%, and as a result of measuring. In the xanthine oxidase inhibition test, 500ppm of water extract showed an effect of 18% and ethanol extract showed an effect of 31.5%. In the tyrosinase inhibition test, 5000ppm of water extract showed an effect of 60% and ethanol extract showed an effect of 85%. In the anti-inflammatory test, the water extract and ethanol extract inhibited the generation of nitric oxide. Conclusions : The results indicated that extract of Forsythia koreana Nakai can be used as a natural ingredients with biological function in cosmetics ingredients.

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.274-277
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• 2010

This study was performed to report the augmented wild populations of Forsythia koreana (Rehder) Nakai and Abeliophyllum distichum Nakai in Korea. Forsythia koreana was found in Budong-myeon, Cheongsong-gun, Gyeongsangbuk-do. The overall size of the natural habitat of Forsythia koreana is $5m{\times}5m$. A total of 12 individuals were recorded in the wild, and 3 of them were in the seedling stage. The wild habitat is in a secondary forest, which is co-dominated by Pinus densiflora Siebold & Zucc and Robinia pseudoacacia L, with 15% coverage. The coverage of the shrub layer is 40% and is dominated by Spiraea prunifolia for. simpliciflora Nakai. The natural habitat of Abeliophyllum disitichum, which is recorded in Uisong-up, Gyeongsangbuk-do, is distributed in sites characterized by boulders along streams, with a patchwork of 15 scattered areas over a habitat size of $50m{\times}15m$. Quercus variabilis Blume is a dominant species in this habitat, with this species dominating at the sub-tree layer. Celtis aurantiaca Nakai also dominates at the shrub layer. The natural ranges of these two species in Korea were mapped based on the terms of the extent of occupancy (EoO).

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.4
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• pp.149-160
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• 1995

We investigated the seasonal changes flower color of 163 deciduous woody landscape plants in the Suwon region from January 1, 1992 to March 20, 1993. The results were as follows; 1. By the month of anthesis of woody landscape plants, only one plant of Hamamelis japonica flowered in February, 15 species in March, 48 species in April, 63 species in May, 23 species in June, 12 species in July, and one plant of Hydrangea paniculata was flowered in August. 2. The flowering period was about 220 days from February 24, 1992 that Hamamelis japonica was anthesis to October 5, 1992 when Hydrangea paniculata was deblossomed. 3. By the flowering period of woody landscape plants, 81 species continued for 11 days through 20 days, and Rosa spp., 118 days, Hibiscus syriacus 'Yungkwang', 80 days, Largerstroemia indica, 65 days, and 6 species continued for 41 through 60 days, 10 species were 31 through 40 days, 43 species were 21 through 30 days, and 20 species were for less than 11 days. 4. The woody landscape plants flowering before leaf spreading, Hamamelis japonica, Abeliophyllum distichum, Prunus mume 'Hwahyangmi', Prunus mume 'Baekkaha', Lindera obtusiloba, Cornus officinalis, Prunus armeniaca. The others were plants with leaves spreading white flowering; Forsythia ovata 'Tetra gold', Forsythia ovata, Corylus hetrophylla, Rhododendron mucronulatum, Magnolia denudata, Forsythia koreana 'Seoul Gold', Forsythia koreana, Magnolia stellata, Acer negundo 'Elegans', Magnolia kobus, Forsythia viridissima 'Bronxensis', Prunus yedoensis, Prunus leveilleana var. pendula, Prunus persica for. albiplena, Prunus tomentosa, Prunus persia, Magnolia liliflora, Prunus glandulosa for. sinensis, Cercis chinensis, Poncirus trifoliata. 5. In terms of flower color based on KBS standard color number, 83 species were white, 44 species wer red, 21 species were yellow, 12 species were violet, and 3 species were green. 6. In terms of the flower color by month. Hamamelis japonica was yellow February. Flower colors in March were : yellow-7 species, red-3 species and white-5 species. Flower colors in April were : White-21 species, red-19 species and yellow-6 species. Flower colors in May were : White-36 species, red-16 species. The white flowers in June were 16 species. Flower colors in July were : white-4 species, red-4 species. 7. The white flower color of woody landscape plants of trees was 35 species. The red flower color was 18 species, yellow flower color was 5 species, violet flower color was 2 species, and green flower color was 3 species. Also the white flower color of woody landscape plants of shrubs was 48 species, red flower color was 25 species, yellow flower color was 17 species and violet flower color was 10 species. 8. The new 'Cultivars' of woody landscape plants are needed to introduced the development of planting design. 9. Present data of illustrated books of plants should be checked by new data that was studied in this research.

• Research in Plant Disease
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• v.29 no.3
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• pp.234-242
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• 2023

In early spring, water-soaked lesions appeared on the petals and leaves of gaenari (Forsythia koreana), and the tissues were necrotic and dry. Cankers appeared on the infected branches around late spring and the above part of a branch withered and died. However, it was very rare that the base of the cankered-branch died. The identical fungi were isolated from the lesions on various tissues, and they grew with white colonies on potato dextrose agar medium. The fungus grew most actively at 23℃ and produced many sclerotia of various sizes. In a pathogenicity assay in which mycelial and sclerotial suspensions were inoculated on each organ of forsythia, it was found that the pathogen infects the flower only, but not the leaves or branches. Symptoms on the flowers spread to the next leaves and branches over time and the infected branches were eventually withered. To identify the isolates, DNA sequences of four phylogenetic markers including ITS, LSU, Tub2, and CAL were analyzed and all isolates were identified as a species in the genus Septotinia. This is not only the first report of gaenari (forsythia) shoot blight caused by the fungus Septotinia sp., but also the first report on the genus Septotinia as a plant pathogen in Korea.

• 한국생태학회:학술대회논문집
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• 1999.05a
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• pp.104.2-104.2
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• 1999

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.727-728
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• 2003

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.105-108
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• 2015

This research is aimed at evaluating the potential abilities of the natural acaricide of F. suspense oil against Tyrophagus putrescentiae and Dermatophagoides spp. Based on the $LD_{50}$ values, in contact bioassay, F. suspense oil (8.19, 3.28, and $4.35{\mu}g/cm^2$) showed acaricidal effects against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively. Fumigant toxicities of F. suspense oil showed similar patterns as those observed with contact toxicities. GC/MS analysis showed the major components of F. suspense oil to be ${\beta}$-pinene (45.88%), myrtenol (13.86%), (+)-${\alpha}$-pinene (13.09%), (-)-trans-pinocarveol (7.34%), sabinene (6.64%) and pinocarvone (4.13%). These findings indicate that F. suspense oil has potential as a natural acaricide.

• Plant Resources
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• v.4 no.3
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• pp.181-188
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• 2001

We tried to introduce two forsythia genes related in lignan biosynthesis, dirigent protein and pinoresinol/lariciresinol (Ph) reductase, into potatoes for accumulation of lignans in transgenic potatoes. We made binary vectors overexpressing dirigent protein gene and P/L reductase gene driven by a CaMV35S promoter and transformed into potatoes via Agrobacterium mediated transformation. And in order to control the metabolic flux of lignan biosynthesis pathway, we tried to inhibit chalcone synthase genes of potatoes by antisense inhibition technique also. We tried to use PCR screening method for selection of transgenic plants of different vectors. We tried to determine and compare lignan contents from different transgenic potato lines.