Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report on the Shoot Blight Disease of Gaenari (Forsythia koreana) Caused by Septotinia sp. in Korea

Septotinia sp.에 의한 개나리 가지마름병(가칭) 발생

  • Ji Hye Woo (Goyang Agricultural Technology Center) ;
  • Mikyeong Kim (Department of Plant Medicine, Chungbuk National University) ;
  • Kun-Woo Lee (Department of Forest Environment Science, Jeonbuk National University) ;
  • Sang-sup Han (Department of Forest Environment Science, Jeonbuk National University) ;
  • Byeongjin Cha (Department of Plant Medicine, Chungbuk National University)
  • 우지혜 (고양시농업기술센터) ;
  • 김미경 (충북대학교 식물의학과) ;
  • 이건우 (전북대학교 산림환경과학과) ;
  • 한상섭 (전북대학교 산림환경과학과) ;
  • 차병진 (충북대학교 식물의학과)
  • Received : 2023.08.03
  • Accepted : 2023.09.12
  • Published : 2023.09.30
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Abstract

In early spring, water-soaked lesions appeared on the petals and leaves of gaenari (Forsythia koreana), and the tissues were necrotic and dry. Cankers appeared on the infected branches around late spring and the above part of a branch withered and died. However, it was very rare that the base of the cankered-branch died. The identical fungi were isolated from the lesions on various tissues, and they grew with white colonies on potato dextrose agar medium. The fungus grew most actively at 23℃ and produced many sclerotia of various sizes. In a pathogenicity assay in which mycelial and sclerotial suspensions were inoculated on each organ of forsythia, it was found that the pathogen infects the flower only, but not the leaves or branches. Symptoms on the flowers spread to the next leaves and branches over time and the infected branches were eventually withered. To identify the isolates, DNA sequences of four phylogenetic markers including ITS, LSU, Tub2, and CAL were analyzed and all isolates were identified as a species in the genus Septotinia. This is not only the first report of gaenari (forsythia) shoot blight caused by the fungus Septotinia sp., but also the first report on the genus Septotinia as a plant pathogen in Korea.

이른 봄에 개나리 꽃잎과 잎에 수침상 병반이 나타나서 조직이 괴저하며 마르고 증상이 가지까지 확산되었다. 감염된 가지에는 궤양이 생기며 5월 초부터 윗부분이 말라 죽는데, 가지 기부까지 말라 죽는 경우는 매우 드물었다. 마름 증상을 보이는 가지 조직으로부터 균을 분리하여 병원성을 확인하였다. 대부분의 시료로부터 같은 균이 분리되었는데, potato dextrose agar 배지에서 흰색 균총을 만들었으며 23℃에서 생육이 가장 활발하였고 크기가 다양한 균핵을 많이 만들었다. 개나리의 각 기관에 균사와 균핵 현탁액을 접종한 병원성 검정에서 병원균은 꽃을 감염하며, 잎이나 가지는 감염하지 않는 것으로 나타났다. 꽃에 나타난 증상은 시간이 지남에 따라 잎과 가지로 확산되며 감염 조직을 고사시켰다. 병원균을 동정하기 위하여 ITS, LSU, Tub2, CAL 등 네 가지 유전자의 염기서열을 분석한 결과 Septotinia속으로 동정되었다. 본 연구는 개나리 가지마름병(가칭)에 대한 국내 첫 보고인 동시에 Septotinia속이 식물병원균임을 밝히는 국내 첫 보고이다.

Keywords

Acknowledgement

This work was supported by the research grant of the Chungbuk National University in 2022.

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