• Journal of the Korean Wood Science and Technology
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• 제29권4호
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• pp.89-96
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• 2001

항균활성이 우수한 회화나무 목부로부터 4종의 isoflavonoid 화합물을 단리하였으며, 기기분석 결과 3종의 isoflavone인 5,7-dihydroxy-4',6-dimethoxyisoflavone(irisolidone)을 비롯하여, 5,7-dihydroxy-4'-methoxyisoflavone(Biochanin A), 7-hydroxy-4'-methoxyisoflavone(formononetin) 그리고 1종의 isoflavanone인 7-hydroxy-4'-methoxyisoflavanone으로 각각 동정하였다. 이들 단리 물질의 항균활성을 배지점적법으로 조사한 결과 4종의 isoflavonoid 공히 낮은 항균활성을 나타내어, 회화나무 목부의 높은 항균활성은 본 실험에서 단리한 물질 이외의 물질이 관여하는 것으로 추정되며 금후 관련물질의 탐색이 필요할 것으로 사료되었다.

• 대한한의학회지
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• 제43권3호
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• pp.79-93
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• 2022

Objectives: We investigated the isoflavone contained in SH003 and its fractions, and the effect of these components on the inhibition of breast cancer. Methods: The isoflavones in solvent fractions of SH003 extract were identified by UPLC-MS and its contents were quantified using HPLC analysis. The estrogenic activity of SH003 or fractions was assessed by ERE luciferase assay in estrogen receptor (ER)-positive MCF-7 cells. To test the breast cancer inhibitory effect, the cell viability was measured using an MTT assay. Results: In this study, we demonstrated that SH003 and fractions contain 4 isoflavones which are calycosin-7-β-D-glucoside, formononetin-7-β-D-glucoside, calycosin, and formononetin. Despite containing isoflavones, estrogen-dependent transcription activity was not altered by both SH003 and fractions. On the other hand, SH003 and fractions inhibited the cell viability of breast cancer. In addition, its isoflavone components also showed reduced cell viability in various breast cancer cells. Conclusions: Overall, the phytoestrogen included in SH003 and fractions did not influence the estrogenic activity, emphasizing the safety of SH003 and fractions in breast cancer treatment.

• 한국식품영양학회지
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• 제36권5호
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• pp.360-367
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• 2023

The major active components of Astragalus membranaceus (AM) are isoflavones, which exist in the form of various glycosides. Nuruk is a traditional fermentation starter in Korea, and is suitable for the biotransformation of isoflavone glycosides because it contains various microorganisms and enzymes. This study was performed to determine changes in the isoflavones and antioxidant properties of AM fermented (AF) with nuruk over 24 hours. AF was sampled after 0, 2, 4, 6, 12, 18, and 24 h of fermentation, and calycosin 7-glucoside, ononin, calycosin, and formononetin content, and the antioxidant properties of AF were analyzed. The total phenolic content increased with fermentation time, and the ABTS radical scavenging activity increased until 6 h of fermentation and then decreased. During fermentation, the isoflavone glycosides decreased significantly as fermentation time increased. The contents of calycosin and formononetin, which are aglycons of calycosin-7-glucoside and ononin, increased from 100.54 ㎍/g to 276.84 ㎍/g and from 56.29 ㎍/g to 123.04 ㎍/g, respectively, at 18 h of fermentation. Significant correlations were observed between fermentation time, isoflavone content, and antioxidant properties. The results of this study showed that fermentation with nuruk is suitable for the biotransformation of isoflavones in AM.

• Journal of Nutrition and Health
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• 제52권5호
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• pp.413-421
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• 2019

본 연구는 황기의 최적의 볶음 공정을 하였을 때 항산화 활성의 변화를 확인하기 위해 DPPH, ABTS radical scavenging assay, 총 폴리페놀 함량 및 calycosin, formononetin과 이의 배당체인 calycosin-7-O-${\beta}$-D-glucoside, Formononetin7-O-${\beta}$-D-glucoside의 분석을 실시하였다. 또한 UVB 처리로 산화적 스트레스를 유발한 HDF 세포에 추출물을 처리하여 DNA의 손상을 억제하는지 확인하였다. DPPH, ABTS radical scavenging assay 는 2R-AM 추출물에서 항산화 활성이 가장 좋았으며, 총 폴리페놀 함량은 1R-AM 추출물이 NR-AM과 비교하였을 때 월등히 증가하였지만, 2R-AM과 3R-AM과는 크게 차이가 없었다. 볶음 공정을 통한 황기는 calycosin와 formononetin의 함량이 2R-AM 추출물에서 가장 높게 측정되었다. HDF 세포에서 추출물들은 $200{\mu}g/mL$까지 독성을 나타내지 않았으며, 추출물 모두 자외선에 의해 손상된 HDF 세포에서 DNA 보호효능을 유의성 있게 증가시켰다. 특히 2R-AM 추출물의 활성은 정상세포와 유사한 수준으로 보호효과를 나타내었다. 결과를 종합해봤을 때, 볶음공정을 통한 황기 추출물은 지표 성분의 변화로 인하여 자외선에 의한 피부보호효과를 증가하였을 것이라 판단되며 이는 건강기능식품이나 화장품 원료로써 가능성을 시사한다.

• Natural Product Sciences
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• 제14권2호
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• pp.131-137
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• 2008

From the 70% EtOH extract of the roots of Astragalus membranaceus (Leguminosae), eleven flavonoid derivatives and a lignan, were isolated and identified as liquiritigenin (1), daidzein (2), formononetin (3), sophorophenolone (4), calycosin (5), methylnissolin (6), isomucronulatol (7), isomucronulatol 7-O-glucoside (8), methylnissolin 3-O-glucoside (9), calycosin 7-O-glucoside (10), (+)-syringaresinol O-${\beta}$-D-glucoside (11), and isomucronulatol 7,2'-di-O-glucoside (12), by spectroscopic methods. This is the second report of the isoflavonoid derivatives sophorophenolone (4) and isomucronulatol 7,2'-di-O-glucoside (12) from a natural source, as well as the first report of compounds liquiritigenin (1), daidzein (2) and (+)-syringaresinol O-${\beta}$-D-glucoside (11) from the species A. membranaceus.

• Natural Product Sciences
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• 제17권4호
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• pp.363-366
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• 2011

Guibi-tang is a traditional medicine used for the treatment of colds. We investigated the levels of several compounds in Guibi-tang before and after fermentation with Lactobacillus and tested their ability to inhibit angiotensin-converting enzyme. Six known compounds (decursin, decursinol angelate, nodakenin, liquiritin, formononetin, and 6-gingerol) and 2 unidentified compounds were detected in Guibi-tang (GB) and fermented Guibi-tang (FGB) by an established HPLC-DAD method. The levels of the 6 known compounds were decreased after fermentation. FGB showed more potent inhibition of angiotensin-converting enzyme activity than GB. In conclusion, fermentation with Lactobacillus affects the content of several compounds in GB and improves its angiotensin-converting enzyme inhibitory activity.

• Archives of Pharmacal Research
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• 제17권4호
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• pp.236-239
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• 1994

The suppressive activity of isoflavonoids against lymphocyte proliferation in vitro was examined. Isoflabvonoid derivatives tested were isflavones isolated from Pueraia radix and synthesized 7-O-substituted biochanin A derivatives. The certain isoflavones such as biochanin A and 2-carbethoxybiochainin A were found to possess the suppressive activity against concanavaline A (Con A)-induced lymphocyte proliferation from mouse spleen. Against mixed lymphocyte culture reaction, biochanin A, 2-carbethoxybiochainin A, daidzein, formononetin, genistein and 7-O-isopropylbiochaninl A showed the suppressive activity at $10^{-5}$ M. However, all isoflavones tested did not show the suppressive activity against lymphocyte proliferation induced by B-cell mitogen, lipopolysaccharide (LPS). In general, isoflavones were revealed to be less active than flavones/flavonols.

• 한국약용작물학회지
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• 제22권2호
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• pp.91-97
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• 2014

The contents of index components in 5 medicinal crops cultivated at different heights in Andong area. The contents of allantoin in Dioscorea batatas Decne and catalpol in Rehmannia glutinosa Liboschitz were not different by cultivation heights (100 ~ 200m). But the contents of index components in Astragalus membranaceus and Angelica acutiloba Kitagawa were higher in samples cultivated at elevated heights. At 700 m of heights, the contents of formononetin and astragaloside I in Astragalus membranaceus were 1.20 and $201.2{\mu}g/g$, respectively. The contents of decursin and nodakenin in Angelica acutiloba Kitagawa were 68.3 and 4.20 mg/g, respectively. Thus Astragalus membranaceus and Angelica acutiloba Kitagawa are recommended to be cultivated in higher altitude.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.217.1-217.1
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• 2003

Phytoestrogen has been used as supplement in order to treat osteoporosis. The representative phytoestrogen, isoflavones, are daidzein, genistein and formononetin which were present highly in legumes. We have studied the quantitative analysis of isoflavones in KUNBO by HPLC. KUNBO, a mixed herbal extract including Astragali Radix and Rhynchosiae nulubilis Semen (Leguminosae), etc., is a nutraceutical candidate for type I osteoporosis. olumn used in HPLC was LUNA 5${\mu}$ C18 (250 ${\times}$4.6mm) (Phenmenex Co.m Torroance, CA, U.S.A.). (omitted)

• 한국식품저장유통학회지
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• 제23권5호
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• pp.680-688
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• 2016

본 연구에서는 상황버섯 균사체를 황기에 배양하여 이용성 및 기능성을 증진시킨 식품 및 소재를 개발하기 위한 연구의 일환으로 기초연구를 실시하였다. 수분함량, pH, 산도 및 환원당을 측정함으로서 균사체 배양 황기의 변화를 관찰한 결과 수분함량 및 pH는 균사체 배양기간이 지남에 따라 유의적으로 증가하는 경향을 보인 반면 산도는 시간이 지남에 따라 오히려 대체적으로 감소하는 경향을 나타냈다. 환원당 함량 측정결과 배양 30일차 황기가 대조구에 비해 약 2배 증가한 0.61 g/100 g으로 가장 높은 함량을 나타냈고, 배양 10, 20일차는 각각 0.36, 0.42 g/100 g 으로 대조구보다 다소 증가한 함량을 나타냈다. 추출용매에 따른 균사체 배양 황기의 추출수율 결과 열수추출물이 40.18~41.99% 범위로 80% 에탄올 추출물의 28.70~33.18%보다 높게 나타났다. 유리당 함량 분석결과 80% 에탄올 추출물 및 열수추출물의 모든 실험구에서 배양기간이 지남에 따라 fructose 및 sucrose의 함량이 감소하는 경향을 나타내었다. 유리아미노산은 필수아미노산 8종을 포함해서 총 17종이 검출되었으며 상황버섯균 배양 황기의 주요 아미노산은 arginine, alanine, aspartic acid 및 valine 으로 확인되었다. 아미노산의 총 함량은 배양기간이 지남에 따라 20일차까지 소폭 증가 한 뒤 30일차 감소하였으며, 그 중 배양 20일차가 121.47 mg%으로 배양일 중 가장 증가한 함량을 보였다. 상황버섯균 배양 황기의 성분 함량을 분석한 결과 열수 추출물의 배양 30일차에서 calycosin-7-o-${\beta}$-d-glucoside은 3.30 mg/100 g, ononin은 2.85 mg/100 g으로 대조구에 비해 각각 5배, 5.1배 감소하였으며, calycosin 및 formononetin 함량은 3.91 mg/100 g, 1.38 mg/100 g으로 대조구에 비해 각각 8.7배와 7.7배 증가한 함량으로 다른 배양일 보다 유의적으로 높은 함량을 나타내었다. 이는 황기에 존재하는 배당체인 calycosin-7-o-${\beta}$-d-glucoside 및 ononin (Formononetin-7-O-${\beta}$-d-glucoside) 성분이 aglycon 형태로 전환이 된 것으로 사료된다. 이러한 분석결과는 상황버섯 균사체 배양을 통한 황기의 이화학적특성 변화를 초래하여 기능성을 증대 시킬 수 있음을 확인하였고, 추후 긍정적 영향을 미칠 수 있는 천연소재로서 산업적 활용이 가능할 것이라 생각된다.