• Proceedings of the Korean Society for Bioinformatics Conference
• /
• 2006.02a
• /
• pp.83-89
• /
• 2006

Cellular functions are carried out by a concerted action of biochemical pathways whose components have genetic interactions. Abnormalities in the activity of the genes that constitute or modulate these pathways frequently have oncogenic implications. Therefore, identifying the upstream regulatory genes for major biochemical pathways and defining their roles in carcinogenesis can have important consequences in establishing an effective target-oriented antitumor strategy We have analyzed the gene expression profiles of human liver cancer samples using cDNA microarray chips enriched in liver and/or stomach-expressed cDNA elements, and identified groups of genes that can tell tumors from non-tumors or normal liver, or classify tumors according to clinical parameters such as tumor grade, age, and inflammation grade. We also set up a high-throughput cell-based assay system (cell chip) that can monitor the activity of major biochemical pathways through a reporter assay. Then, we applied the cell chip platform for the analysis of the HCC-associated genes discovered from transcriptome profiling, and found a number of cancer marker genes having a potential of modulating the activity of cancer-related biochemical pathways such as E2F, TCF, p53, Stat, Smad, AP-1, c-Myc, HIF and NF-kB. Some of these marker genes were previously blown to modulate these pathways, while most of the others not. Upon a fast-track phenotype analysis, a subset of the genes showed increased colony forming abilities in soft agar and altered cell morphology or adherence characteristics in the presence of purified matrix proteins. We are currently analyzing these selected marker genes in more detail for their effects on various biological Processes and for Possible clinical roles in liver cancer development.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.3
• /
• pp.330-335
• /
• 2005

We have investigated the effects of Bu-Zhong-Yi-Qi- ang on the differentiation of murine bone marrow cells in methylcellulose culture. GM-CSF and IL-3 supported primarily the formation of granulocyte/mac-rophage colony formation. However, the addition of Bu-Zhong-Yi-Qi-Tang extract yielded a significant increase in the numbers of colonies and differentiated cells in the presence of GM-CSF and IL-3. We have analyzed CD11b (Mac-1) expression of differentiated cells from bone marrow by staining with monoclonal anti-CD11b antibody. The majority of colony-forming cells were in CD11b/sup +/ population. Also Bu-Zhong-Yi-Qi-Tang extract promoted the production of IL-6 and nitric oxide by macrophages. These results demonstrate that extract of Bu-Zhong-Yi-Qi-Tang, a prescription of traditional oriental medicine, is effective in supporting macrophage potential of the primary colonies.

• Journal of the Korean GEO-environmental Society
• /
• v.18 no.7
• /
• pp.13-20
• /
• 2017

Soil nailing is ground reinforcement method using the shear strength of ground and the pullout shear resistance force of nail. It is mainly used for reinforcement of cut slopes, earth retaining structures and retaining walls, etc. It may be designed considering the pullout resistance of nail in the case of earth retaining structure and retaining wall, but it should be designed considering not only pullout resistance but also shear and bending resistance in the case of slope. However, conservative designs considering only pullout resistance are being done and most of the studies are about increasing pullout resistance by improving of material, shape and construction method of nail. Actually, Shear bending deformations occur centering on the active surface in ground reinforced with the nail. The grout with relatively low strength is destroyed and separated from the reinforcing material. As a result, the ground is collapsed while reducing the frictional resistance rapidly. Therefore, it is necessary to develop the method to increase the shear resistance while preventing separation of nail and grout body. In this study, an experimental study was conducted on new soil nailing method which can increase shear resistance by forming protrusions through pressurized grouting after installing a packer on the outside of deformed bar.

• Journal of Korean Home Economics Education Association
• /
• v.31 no.3
• /
• pp.117-134
• /
• 2019

This study aims to explore the effects of the parenting education program for high-school maladjusted students. To this purpose, a parenting education program for adolescents developed by the Ministry of Gender Equality and Family(MOGEF) was applied to ten high-school maladjusted students in an alternative(Dae-an) class in Jeju. Findings from class observations and interviews with participants are as follows. First, students with school maladjustment recognized importance and necessity of positive self-concepts and had an opportunity for self-growth through parenting education program. Second, students with school maladjustment obtained understanding about their family of origin and confidence for forming their future families. Third, advance learning about meanings and roles of being a parent provided students with opportunities to know importance of responsible sexual behavior. Fourth, practices of basic care with a replica of a new-born baby enhanced participation and concentration level of students with school maladjustment which became extremely effective in obtaining necessary knowledge for the basic care. Fifth, information provision for parent roles according to children's different developmental levels let students recognize that parent's roles change as their children grow and opened a way to grasp the need of parent education. Sixth, education and repetitive practices on reflective listening helped students enhance communication skills which entailed expanding their human relations. Seventh, passing-down of emotion and understanding on child abuse allowed students recognize risks of child abuse as well as the importance and need of skills for controlling emotion. This study sheds lights on the feasibility and effectiveness of a parenting education program for maladjusted or vulnerable students as an effective program for school drop-out prevention.

• Journal of the Korean Institute of Gas
• /
• v.20 no.4
• /
• pp.78-84
• /
• 2016

In this work, finite element investigations were carried out to optimize reverse drawing process design for manufacture of high-capacity aluminum liner used in fuel cell vehicle. The tensile tests with aluminum alloy Al6061 annealed at $350^{\circ}C$ were carried out to obtain the flow stresses. In order to estimate more accurate flow stresses after necking, the flow stresses were estimated from the comparison of load vs. displacement curves which were obtained from experimental and simulation results of tensile tests. In case of finite element analyses of reverse drawing processes, it was focused on the effects of process designs such as punch and die designs, blank holding force, drawing ratio and the clearance between the punch and blank holder on the generation of wrinkle and fracture of the blank and partially heated punch. However, it was revealed that experimental results still show the fracture at the end of 2nd drawn cup, although partially heated punch is used. Nevertheless, the drawn cup can be used because the sufficient length of the drawn cup for the next flow forming process and spinning process was obtained.

• KSBB Journal
• /
• v.25 no.6
• /
• pp.520-526
• /
• 2010

In this work we have investigated the production of catalase from Bacillus sp. strains, which were screened and identified from soil. These strains were cultivated in shaking flasks with tryptic soy broth (TSB) at $30^{\circ}C$ and 200 rpm. Effects of the temperature and pH on the stability of the native catalase and whole cell viability were studied in the temperature range of $25-60^{\circ}C$ and the pH range of 7-13. Korean natural zeolite was added to culture medium and mixed with microorganisms for 24 hours. The native catalase maintained its activity over $50^{\circ}C$. The enzyme acitiviy of the catalase from Bacillus flexus BKBChE-3 was highest among the Bacillus sp. strains studied. Bacillus flexus BKBChE-3 and immobilized Bacillus cells have survived under extreme conditions of over $50^{\circ}C$ and pH 12. 60 mL of 10.5 mM $H_2O_2$ solution were entirely removed within 1 hour with catalase produced from Bacillus sp. on the flask. When Bacillus cells were immobilized on Korean natural zeolite, colony forming unit of Bacillus flexus BKBChE-3 was increased and high efficiency of hydrogen peroxide removal was observed.

• Korean journal of applied entomology
• /
• v.45 no.2 s.143
• /
• pp.217-226
• /
• 2006

As the results achieved by the evaluation of toxicities on an aphid parasitoid, Aphidius colemani by 79 pesticides registered as horticultural pesticide and 4 adjuvants far pest control, 6 insecticides including a-cypermethrin, 13 fungicides including metalaxyl-M+mancozeb and 4 acaricides including bifenazate showed low toxicity against A. colemani adult. Low toxicity was showed in all the 4 adjuvants as well. In residual toxicity test from 40 pesticides which showed toxicity more than 50%, A. colemani was safe from 11 pesticides from the 3th day after treatment, 7 pesticides from the 5th day after treatment and 14 pesticides from the 7th day after treatment, respectively. But, chlorpyrifos-methyl, diflubenzuron+chlorpyrifos, etofenprox+diazinone and imidachloprid+chlorpyrifos showed high toxicities reaching 100%, 97.7%, 100% and 100% respectively, even from the 7th day after treatment. To evaluate the control effect by A. colemani against Myzus persicae in a greenhouse, A. colemani was released at parasitoid versus aphids rates of 1:50 and 1:100 when the population of M persicae was 50 per plant. After release, aphids population remained steady for 20 days after release at the level of around 60 aphids per plant. During the investigation, insecticides fur thrips control and fungicides for powdery mildew control were treated, but didn't affect the mummy forming of A. colemani. It may be suggested from these results that the selected insecticides, fungicides, acaricides and adjuvent could be incorporated into the integrated M. persicae management system with A. colemani on greenhouse cultivation.

• Culinary science and hospitality research
• /
• v.19 no.5
• /
• pp.206-224
• /
• 2013

The purpose of this study was to examine the formation of customer's repeated use that is real long-term commitment in franchise/chain coffee shops. 457 effective samples were analyzed using PASW 18.0 and AMOS 18.0. The results of the analysis of the relationships between user experience, customer satisfaction, customer commitment and repeated use are as follows. First, it was presented that atmosphere, price fairness and coffee product quality had a positive impact on customer satisfaction. Second, customer satisfaction had a positive impact on customer commitment and repeated use. Finally, there was the mediating effect of continuous commitment between customer satisfaction and repeated use. Based on the results of the above analysis, the managerial implications for increasing repeated use of coffee shops are as follows. First, the strategy for enhancing performance of user experience is to try to improve atmosphere(facilities, ambient/lighting), price fairness, coffee product quality(taste, flavor, temperature). Second, the strategy for forming repeated use is to enhance customer commitment(affective commitment, continuous/normative commitment).

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.36 no.2
• /
• pp.99-103
• /
• 2010

Waxes, or long-chain hydrocarbons, may be obtained naturally from animals, vegetables, and mineral waxes, or may be synthesized. The oil-wax gels are widely applied to lots of cosmetics such as lipsticks. For example, the lipstick texture is strongly dependent on the glossiness of the oil-wax gels. Extensive research has been carried out to investigate the lattice structure of wax mixture in pure solvents (hydrocarbons) and defined mixtures. However, only a limited amount of work has been published on the lattice structure of wax matrixes in undefined mixtures. The objective of this study was to investigate the relationship between the lattice structure of ceresin wax and different wax mixtures and the glossiness of oil-wax gels. Recently visual factors such as the glossiness of skin are generally known as the words to express the beauty. The mechanism of glossiness has been suggested to understand the changes that occur in the lattice structure of the wax matrixes when they are forming gels and also the effects of the nature of solvent. The present work investigates the lattice structure of the wax matrixes and glossiness of oil-wax gels obtained from ceresin and microcrystalline wax as well as of the gels formed by different waxes in solvent.

• Journal of Convergence for Information Technology
• /
• v.7 no.6
• /
• pp.259-265
• /
• 2017

This study reviewed the existing studies in order to set up a strategy that can achieve the trust and business performance of the franchisers, who are actually operating franchised business, breaking from the research trend centered around franchise headquarters and added the factors of the franchise headquarters' strategy for expanding franchises, including word-of-mouth communication and advertising & publicity and added the exercise of recruitment agency outsourcing, flagship store, recently utilized often as preceding variables to verify the causal relation between the trust of the franchiser and the business performance of the franchise. The implications of this study, it can be concluded that the government authority for the attraction of healthy franchises can continuously attract franchises and enter the market can continuously attract franchises by increasing the trust of the franchises, and powerfully investing and managing them for the effort through word-of-mouth marketing. It is expected that this study will be helpful in the establishment of a business strategy for forming a continuous relationship as well as the franchise head office's strategy for the recruitment of new franchises.