Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Stimulation of Macrophage Differentiation by Bu-Zhong-Yi-Qi- Tang Extract

보중익기탕 추출물의 대식세포 분화 유도 효과

  • Kang, Hye-In (Dept. of Food and Nutrition, College of Natural Science, Sunchon National University) ;
  • Shin, Sung-Hae (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Cho, Young-Sook (Dept. of Food and Nutrition, College of Natural Science, Sunchon National University) ;
  • Cho, Sung-Ki (Dept. of Food Irradiation, Korea Atomic Energy Research Institute) ;
  • Byun, Mung-Woo (Dept. of Food Irradiation, Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Natural Science, Sunchon National University)
  • 강혜인 (순천대학교 식품영양학과) ;
  • 신성해 (순천대학교 생물학과) ;
  • 조영숙 (순천대학교 식품영양학과) ;
  • 조성기 (원자력연구소 식품ㆍ생명공학팀) ;
  • 변명우 (원자력연구소 식품ㆍ생명공학팀) ;
  • 이성태 (순천대학교 생물학과)
  • Published : 2005.03.01
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Abstract

We have investigated the effects of Bu-Zhong-Yi-Qi- ang on the differentiation of murine bone marrow cells in methylcellulose culture. GM-CSF and IL-3 supported primarily the formation of granulocyte/mac-rophage colony formation. However, the addition of Bu-Zhong-Yi-Qi-Tang extract yielded a significant increase in the numbers of colonies and differentiated cells in the presence of GM-CSF and IL-3. We have analyzed CD11b (Mac-1) expression of differentiated cells from bone marrow by staining with monoclonal anti-CD11b antibody. The majority of colony-forming cells were in CD11b/sup +/ population. Also Bu-Zhong-Yi-Qi-Tang extract promoted the production of IL-6 and nitric oxide by macrophages. These results demonstrate that extract of Bu-Zhong-Yi-Qi-Tang, a prescription of traditional oriental medicine, is effective in supporting macrophage potential of the primary colonies.

본 실험에서는 생쥐에서 분리한 골수 세포의 분화에 보중익기탕 추출물이 미치는 영향을 알아보았다. GM-CSF와 IL-3는 대식세포의 형성을 자극하는데 관여한다. 보중익기탕 추출물을 첨가하였을 때 GM-CSF와 IL-3로 분화하는 colony의 수를 증가시키는 것을 알 수 있었으며 anti-CD11b 항체로 염색한 결과 분화한 세포는 대식 세포의 특이적 세포표면 단백질 인 CD11b(Mac-1)를 발현하고 있었다. 또한 보중익기탕 추출물은 대식세포가 생산하는 IL-6와 일산화질소(nitric oxide)의 분비량도 증가시키는 것을 알 수 있었다. 이상의 실험 결과, 오래 전부터 전통 처방제로 사용되어온 보중익기탕 추출물은 대식세포의 분화와 증식을 유도하는데 효과가 있는 것으로 생각된다.

Keywords

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