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Immobilization of Bacillus sp. Strains, Catalase Producing Bacteria and Their Hydrogen Peroxide Removal Characteristics  

Han, Kyung-Ah (Department of Material and Biochemical Engineering, Chonnam National University)
Jang, Yun-Hee (Department of Advanced Chemicals, Chonnam National University)
Rhee, Jong-Il (School of Applied Chemical Engineering, Chonnam National University)
Publication Information
KSBB Journal / v.25, no.6, 2010 , pp. 520-526 More about this Journal
Abstract
In this work we have investigated the production of catalase from Bacillus sp. strains, which were screened and identified from soil. These strains were cultivated in shaking flasks with tryptic soy broth (TSB) at $30^{\circ}C$ and 200 rpm. Effects of the temperature and pH on the stability of the native catalase and whole cell viability were studied in the temperature range of $25-60^{\circ}C$ and the pH range of 7-13. Korean natural zeolite was added to culture medium and mixed with microorganisms for 24 hours. The native catalase maintained its activity over $50^{\circ}C$. The enzyme acitiviy of the catalase from Bacillus flexus BKBChE-3 was highest among the Bacillus sp. strains studied. Bacillus flexus BKBChE-3 and immobilized Bacillus cells have survived under extreme conditions of over $50^{\circ}C$ and pH 12. 60 mL of 10.5 mM $H_2O_2$ solution were entirely removed within 1 hour with catalase produced from Bacillus sp. on the flask. When Bacillus cells were immobilized on Korean natural zeolite, colony forming unit of Bacillus flexus BKBChE-3 was increased and high efficiency of hydrogen peroxide removal was observed.
Keywords
Catalase; Hydrogen peroxide; Bacillus sp.; Immobilization; Zeolite;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Murthy, M. R. N., T. J. III. Reid, A. Sicignano, N. Tanaka, and M. G. Rossmann (1981) Structure of beef liver catalase. J. Mol. Biol. 152: 465-499.   DOI
2 Shinonaga, M.-A., K. Yoshihide, S. Kikuo, and Y. Tsuneo (1996) Continuous production of phospholipase D by Streptomyces lydicus D-121 immobilized with cross-linked chitosan bead. J. Ferment. bioeng. 81: 310-314.   DOI   ScienceOn
3 Çetinus, S. A. and O. H. Nursevin (2003) Immobilization of catalase into chemically crosslinked chitosan beads. Enzyme Microb. Technol. 32: 889-894.   DOI   ScienceOn
4 Mo, S.-Y., H.-K. Chang, K.-J. Lee, G.-E. Jang, and J.-R. Sohn (2000) Measurement of the quantity of hydrogen peroxide produced in the ultrasound-irradiated aqueous solution of organic compounds. J. Korean Soc. Environ. Eng. 22: 61-71.
5 Lee, D. H., S. J. Kim, and H. Moon (1999) Preparation of a clinoptilolite-type korean natural zeolite. Korean J. Chem. Eng. 16: 525-531.   DOI
6 Lee, S.-H., J.-H. Lee, D. G. Kim, C. S. Lee, K. S. Kang, and I. H. Kim (2008) Simultaneous removal of ammonium and nitrate by natural zeolite and bacteria. Korean Chem. Eng. Res. 40: 971-976.
7 Nakamura, H., K. Samejima, and T. Zenzo (1974) A capillary tube method for counting viable cells of Bifidobacterium bifidum growth in a solid medium. Japan J. Microbiol. 18:135-138.   DOI
8 Zweifel, C., J. E. Muehlherr, M. Ring, and R. Stephan (2005) Influence of different factors in milk production on standard plate count of raw small ruminant's bulk-tank milk in Switzerland. Small Rumin. Res. 58: 63-70.   DOI   ScienceOn
9 Costa, S. A., T. Tzanov, A. F. Carneiro, A. Paar, G. M. Gὕbitz, and A. C. Paulo (2002) Studies of stabilization of native catalase using additives. Enzyme Microb. Technol. 30: 387-391.   DOI   ScienceOn
10 (2001) Treatment of seafood wastewater by intermittently aerated activated sludge system with zeolite addition. J. Korean Ind. Eng. Chem. 12: 410-414.
11 Lee, H. S.(2002) Wastewater treatment in a hybrid biological reactor using powered minerals: effects of organic loading rates on COD removal and nitrification. Process Biochem. 38: 81-88.   DOI   ScienceOn
12 Kourkoutas, Y., A. Bekatorou and A. A. Koutinas (2004) Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiol. 21: 377-397.   DOI   ScienceOn
13 Kam, S.-K., D.-S. Kim, and M.-G. Lee (1999) Comparison of removal performances of divalent heavy metals by natural and pretreated zeolite. J. Korean Environ. Sci. Soc. 8: 399-409.
14 Han, K.-A. and J. I. Rhee (2009) Isolation and characterization of catalase-producing bacteria from soil. Korean J. Biotechnol. Bioeng. 24: 508-514.   과학기술학회마을
15 Heo, B. O., D. C. Lee, and H. J. Shin (2003) Catalase production by membrane process for treatment of industrial wastewater containing hydrogen peroxide. Korean J. Biotechnol. Bioeng. 18: 186-189.   과학기술학회마을
16 Dwight, L. B. (1953) Production of catalase from mold. US Patent 2,605,069.
17 Hans, E. D. (1961) Method of extracting catalase from liver. US Patent 2,992,167.
18 Yang, H. S., H. C. Yang, and Y. Tani (1988) Catalase from Aspergillus niger KUF-04. Korean J. Appl. Microbiol. Bioeng. 16: 193-198.
19 Yu, S.-J., S.-Y. Yu, and K.-Y. Lee (2001) Influence of algitation speed on cell growth in the aerobic yeast fermentation of pulverized liquid food waste for probiotic feed production. J. KOWREC. 9: 99-104.
20 Cetinus, S. A. and H. N. Oztop (2000) Immobilization of catalase on chitosan film. Enzyme Microb. Technol. 26: 497-501.   DOI   ScienceOn
21 Costa, S. A., T. Tzanko, P. Andreas, G. Marinka, M. G. Georg, and C.-P. Artur (2001) Immobilization of catalase from Bacillus SF on alumina for the treatment of textile bleaching effluents. Enzyme Microb. Technol. 28: 815-819.   DOI   ScienceOn
22 Hidalgo, A., B. Lorena, L.-G. Fernando, M. Renata, B. José, F.-L. Foberto, and H. G. José (2003) Design of an immobilized preparation of catalase from Thermus thermophilus to be used in a wide range of conditions. structural stabilization of a multimeric enzyme. Enzyme Microb. Technol. 33: 278-285.   DOI   ScienceOn
23 Norton, S. and T. D'Amore (1994) Physiological effects of yeast cell immobilization : application for brewing. Enzyme Microb. Technol. 16: 365-375.   DOI   ScienceOn
24 Park, J. K. and H. N. Chang (2000) Microencapsulation of microbial cells. Biotechnol. Adv. 18: 301-319.
25 Kourkoutas, Y., A. Bekatorou, I. M. Banat, R. Marchant, and A. A. Koutinas (2004) Immobilization technologies and support materials suitable in alcohol beverages production : a review. Food Microbiol. 21: 377-397.   DOI   ScienceOn
26 Kim, K., K.-I. Jang, C.-H. Kim, and K.-Y. Kim (2002) Optimization of culture conditions and encapsulation of Lactobacillus fermentum YL-3 for probiotics. Korean J. Food Sci. Technol. 34: 255-262.   과학기술학회마을