• KSBB Journal
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• 제26권2호
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• pp.100-106
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• 2011

Strain BCNU 5011 was isolated from forest soil samples collected in the Taebaek mountain in the Gangwon province, Korea. The biochemical, physiological and 16S rRNA sequence analysis strongly indicated that this isolate was most closely related to Paenibacillus polymyxa. A maximum production level of antimicrobial substances of Paenibacillus sp. BCNU 5011 was achieved under aerobic incubation at $30^{\circ}C$ for 3 days in SST broth.Paenibacillus sp. BCNU 5011 showed a broad spectrum of activity against Gram positive and Gram negative bacteria, including methicllinresistant Staphylococcus aureus (MRSA). Paenibacillus sp. BCNU 5011 was also shown to inhibit the growth of different potential human pathogenic bacteria and fungi in vitro. Peptide extract showed better antimicrobial activity than solvent extracts. But active antimicrobial compounds might be included in both peptide extract and solvent extracts. Further separation, purification and identification of active principles leads project to develop antimicrobial agents and anti-MRSA agents.

• KIEAE Journal
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• 제10권1호
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• pp.3-10
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• 2010

Riverside forests make a river bank stable because trees of them hold together the stone and soil by roots and decrease the speed of running fluid by trunks. So they become known to have positive effects on flood prevention. So This study will be a basic study to preserve and restore of riverside forests. The goal of this study is to classify types of Han riverside forests between Yangpyeng and Yeoju gun. and find out sites of planting. Results of this study can be summarized as follows; The evaluation indicators were set up based on literature review and site survey. Two indicator categories were developed: natural environment and human environment. And they were divided into 5 sub-categories for calculating weights. As for the major indicator categories, the weighted index of natural environment is at 0.5. And the weighted index of human environment is at 0.5 followed by access at 0.15, the range of user at 0.15, cultivated land at 0.1 and legislation at 0.1. This study selected 53 sites for riverside forests planting. They were classified with types of bank(11), level-upped riverside(32), island(10). The amount of the length of 11 bank types is 23,050m, the area of 32 level-upped riverside types is $4,490,000m^2$ and the area of 10 island types is $4,590,000m^2$. After the evaluation of 53 riverside forests, this study selected 12 sites of riverside forests. They were two bank types, nine level-upped riverside types, and one island type. Rebuilding riverside forests are to accomplish the green network which links and divides region. It will be one of the best ecological methods to construct friendly environmental region.

• 한국식품영양과학회지
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• 제38권6호
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• pp.801-806
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• 2009

본 연구는 transglutaminase 생산능이 우수한 토양유래방선균 strain YK-2를 TGase 최적생산배지에서 $28^{\circ}C$, 5일간 배양하여 얻은 배양여액으로 본 효소의 정제 및 정제된 효소의 효소화학적 특성에 관하여 검토한 것이다. 본 효소의 정제는 50% methanol precipitation, DEAE-Sephadex column chromatography의 정제 절차를 거쳐 56.5%의 수율로 정제되었고, 정제된 효소의 순도는 12.5% SDS-PAGE에서 단일 밴드를 나타내어, 서브유닛트의 분자량이 약 45,000 dalton으로 추정되는 호모형 효소인 것을 알 수 있었다. 정제된 TGase의 생화학적 제 특성을 검토한 결과, 등전점은 pH $6.0{\sim}7.0$ 부근에 있는 것으로 나타났으며, 본 효소의 기질인 CBZ-L-Gln-Gly 농도에 대한 Km치는 18.5 mM으로 추산되었다. 또한 금속이온 및 저해제의 영향으로는 $Hg^{++}$에 의해서 본 효소의 활성이 강하게 저해되었으나, DTT 및 mercaptoethanol에 의해 각각 293% 및 219% 활성이 증가하였다.

• 한국안전학회지
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• 제27권6호
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• pp.172-177
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• 2012

In areas around Deoksan Li and Deokjeon Li, Inje Eup, Inje Gun, located between $38^{\circ}2^{\prime}55^{{\prime}{\prime}}N$ and $38^{\circ}5^{\prime}50^{{\prime}{\prime}}N$ in latitude and $128^{\circ}11^{\prime}20^{{\prime}{\prime}}E$ and $128^{\circ}18^{\prime}20^{{\prime}{\prime}}E$ in longitude, large-sized avalanche disasters occurred due to Typhoon Ewiniar in 2006. As a result, 29 people were dead or missing, along with a total of 37.25 billion won of financial loss(Gangwon Province, 2006). To evaluate such landslide and debris flow risk areas and their vulnerability, this study applied a technique called 'Weight of Evidence' based on GIS. Especially based on the overlay analysis of aerial images before the occurrence of landslides and debris flows in 2005 and after 2006, this study extracted 475 damage-occurrence areas in a shape of point, and established a DB by using such factors as topography, hydrologic, soil and forest physiognomy through GIS. For the prediction diagram of debris flow and landslide risk areas, this study calculated W+ and W-, the weighted values of each factor of Weight Evidence, while overlaying the weighted values of factors. Besides, the diagram showed about 76% in prediction accuracy, and it was also found to have a relatively high correlationship with the areas where such natural disasters actually occurred.

• 한국균학회지
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• 제38권1호
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• pp.8-15
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• 2010

상록활엽수림에서 출현 균류를 알아보기 위해, 제주도 선흘곶 동백동산에서 야생버섯의 출현과 서식처를 조사하였다. 버섯 샘플은 2007년 6월부터 2008년 12월까지 4개 조사구로 구성된 500 내에서 채집되었다. 버섯은 69과 178종이 동정되었고, 이중 28속 50종은 제주도에서 처음 보고되는 종이고, 선홀광대버섯(Amanita pseudogemmata)은 한국에서 처음으로 보고되는 종이었다. 버섯 종들은 서식처별로 6개 그룹으로 나누어졌다(죽은 나무에 44종, 낙엽에 29종, 토양 표면에 99종, 동물 똥에 2종, 곤충 또는 버섯에 2종 그리고 이끼에 2종).

• The Plant Pathology Journal
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• 제27권1호
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• pp.59-67
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• 2011

A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

• Journal of Plant Biotechnology
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• 제29권3호
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• pp.185-188
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• 2002

유자의 성숙종자를 MS 기본배지에 치상하여 종자의 내피에서 주심조직 유래의 희고 부서지기 쉬운 배발생캘러스가 1.2%의 빈도로 형성되었다. 이 캘러스는 1 mg/L 2,4-D를 첨가한 MS배지에서 증식되었다. 증식된 캘러스를 0.1 mg/L kinetin을 첨가한 MS 배지에 옮겼을 때 많은 수의 체세포배가 형성되었다. 배발생캘러스를 1 mg/L 2,4-D를 첨가한 액체 배지에 넣어 배발생 현탁배양계를 확립하였다. 배양된 현탁배양세포를 0.5 mg/L ABA를 첨가한 고체배지에 평판하였을 때 높은 빈도로 체세포배로 발달하였으며 MS 기본배지 혹은 1 mg/L kinetin 첨가배지에서 소식물체로 발달하였다. 소식물체는 성공적으로 토양으로 옮겨서 온실에서 육성되었다.

• 한국측량학회지
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• 제32권4_1호
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• pp.281-292
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• 2014

본 연구는 산사태 관련 인자를 달리하여 산사태 취약성을 분석한 후, 정확도를 비교하고자 한다. 이를 위해 항공사진을 이용하여 산사태 위치를 추출하였고, 항공 LiDAR와 수치지도를 이용한 지형인자, 각종 주제도를 이용한 토양, 임상, 토지피복 인자를 추출하여 공간데이터베이스를 구축하였다. 산사태 취약성 지도는 로지스틱 회귀분석과 빈도비를 이용하여 산사태 취약지수를 산정하는 것에 의해 작성되었다. 분석결과, 항공 LiDAR와 수치지도의 상관관계는 거의 일치하였으며, 각 방법별로 작성된 산사태 취약성 지도 사이에는 강한 상관관계가 존재하였다. 각 방법별로 작성된 산사태 취약성 지도는 높은 예측 정확도를 보였다. 특히, 빈도비와 항공 LiDAR를 이용할 경우 성능이 더욱 향상되었다. 이를 통해 항공 LiDAR 자료는 효과적인 산사태 발생 예측 및 피해저감대책을 수립하는데 기여할 것으로 판단된다.

• 대한원격탐사학회지
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• 제26권1호
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• pp.47-57
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• 2010

본 연구의 목적은 2006년 태풍 에위니아, 빌리스, 개미와 집중호우로 인해 많은 산사태가 발생한 진부면 지역을 대상으로 항공사진을 이용한 산사태 탐지 및 인공신경망과 GIS를 이용한 산사태 취약성을 분석하는데 있다. 산사태 위치는 산사태 발생 전후의 항공사진을 판독 후 현장에서 확인하였다. 취약성 분석을 위해 지형, 지질, 토양, 임상, 선구조, 토지이용도 등의 자료는 공간 데이터베이스로 구축하였다. 산사태와 관련 요인들간의 상대적 가중치는 인공신경망의 역전파 알고리즘을 이용하여 결정하였다. 그 결과 경사방향과 경사는 다른 요인들 보다 1.2~1.5배 높게 나타났다. 이 가중치를 이용하여 취약성도를 작성 후 분석에 사용하지 않은 산사태 위치와 비교하여 검증하였다. 그 결과 예측 정확도는 81.44%로 나타났다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2016년도 학술발표회
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• pp.204-204
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• 2016

Thailand had set the National Water Management Strategy which covered main six areas in the next 12 years, i.e., by priority: (1) water for household, (2) water for agricultural and industrial production, (3) water for flood and drought management, (4) water for quality issue, (5) water from forest conservation and soil erosion protection, (6) water resources management. However due to the climate change impact, there is a question for all strategies is whether to complete this mission under future climate change. If the impact affects our target, we have to clarify how to mitigate or to adapt with it. Vulnerability assessment was conducted under the framework of ADB's (with the parameters of exposure, sensitivity and adaptive capacity) and the assessments were classified into groups due to their different characteristic and the framework of the National Water Management Strategy, i.e., water supply (rural and urban), water for development (agriculture and others), water disasters (floods (flash, overflow), drought, water quality). The assessments identified the parameters concerned and weight factors used for each groups via expert group discussions and by using GIS mapping technology, the vulnerability maps were produced. The maps were verified with present water situation data (floods, drought, water quality). From the analysis result of this water resources management strategy, we found that 30% of all projects face the big impacts, 40% with low impact, and 30% for no impact. It is clear that water-related agencies have to carefully take care approximately 70% of future projects to meet water resources management strategy. It is recommended that additional issues should be addressed to mitigate the impact from climate risk on water resource management of the country, i.e., water resources management under new risk based on development scenarios, relationship with area-based problems, priority definition by viewpoints of risk, vulnerability (impact and occurrence probability in past and future), water management system in emergency case and water reserve system, use of information, knowledge and technology in management, network cooperation and exchange of experiences, knowledge, technique for sustainable development with mitigation and adaptation, education and communication systems in risk, new impact, and emergency-reserve system. These issues will be described and discussed.