Browse > Article
http://dx.doi.org/10.7841/ksbbj.2011.26.2.100

Characterization of an Indigenous Antimicrobial Substance-producing Paenibacillus sp. BCNU 5011  

Choi, Hye-Jung (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University)
Kim, Ya-Ell (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University)
Bang, Ji-Hun (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University)
Kim, Dong-Wan (Department of Microbiology, Changwon National University)
Ahn, Cheol-Soo (Cho-A Pharm. Co, LTD.)
Jeong, Young-Kee (Department of Biotechnology, Dong-A University)
Joo, Woo-Hong (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University)
Publication Information
KSBB Journal / v.26, no.2, 2011 , pp. 100-106 More about this Journal
Abstract
Strain BCNU 5011 was isolated from forest soil samples collected in the Taebaek mountain in the Gangwon province, Korea. The biochemical, physiological and 16S rRNA sequence analysis strongly indicated that this isolate was most closely related to Paenibacillus polymyxa. A maximum production level of antimicrobial substances of Paenibacillus sp. BCNU 5011 was achieved under aerobic incubation at $30^{\circ}C$ for 3 days in SST broth.Paenibacillus sp. BCNU 5011 showed a broad spectrum of activity against Gram positive and Gram negative bacteria, including methicllinresistant Staphylococcus aureus (MRSA). Paenibacillus sp. BCNU 5011 was also shown to inhibit the growth of different potential human pathogenic bacteria and fungi in vitro. Peptide extract showed better antimicrobial activity than solvent extracts. But active antimicrobial compounds might be included in both peptide extract and solvent extracts. Further separation, purification and identification of active principles leads project to develop antimicrobial agents and anti-MRSA agents.
Keywords
Paenibacillus polymyxa; Antimicrobial activity; Human pathogenic fungi; Methicllin-Resistant Staphylococcus aureus;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Saito, N. and M. Nei (1987) The neighbor-joining method, a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 79: 426-434.
2 Thompson, J. D., D. G. Higgins, and T. J. Gibson (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nuclic. Acids Res. 22: 4673-4680.   DOI   ScienceOn
3 Perez, C., M. Pauli, and P. Bazerque (1990) An antibiotics assay by agar well diffusion method. Acta. Biol. Med. Exp. 15: 113-115.
4 Falagas, M. E. and S. K. Kasiakou (2006) Toxicity of polymyxins: a systematic review of the evidence from old and recent studies. Crit. Care. 10: R27.   DOI
5 Tankovic, J., P. Legrand, G. De Gatines, V. Chemineau, C. Brun- Buisson, and J. Duval (1994) Characterization of a hospital outbreak of imipenemresistant Acinetobacter baumannii by phenotypic and genotypic typing methods. J. Clin. Microbiol. 32: 2677-2681.
6 Sneath, P. H. A. (1986) Endospore-forming gram-positive rods and cocci, pp. 1104-1139. In: P. H. A. Sneath, N. S. Mair, M. E. Sharpe, J. G. Holt (eds.). Bergey's Manual of Systematic Bacteriology, Vol. 2, Williams & Wilkins, Baltimore.
7 Cho, Y. S., N. L. Schiller, H. Y. Kahng, and K. H. Oh (2007) Cellular responses and proteomic analysis of Escherichia coli exposed to green tea polyphenols. Curr. Microbiol. 53: 501-506.
8 Martin, N. I., H. Hu, M. M. Moake, J. J. Churey, R. Whittal, R. W. Worobo, and J. C. Vederas (2003) Isolation, structural characterization, and properties of mattacin (polymyxin M), a cyclyc peptide antibiotic produced by Paenibacillus kobensis M. J. Biol. Chem. 278: 13124-13132.   DOI
9 Senturk, S. (2005) Evaluation of the anti-endotoxic effects of polymyxin-E (colistin) in dogs with naturally occurred endotoxic shock. J. Vet. Pharm. Ther. 28: 57-63.   DOI   ScienceOn
10 Pedersen, M. F., J. F. Pedersen, and P. O. Adsen (1971) A clinical and experimental comparative study of sodium colistimethate and polymyxin B sulfate. Invest. Urol. 9: 234-237.
11 Govaerts, C., J. Orwa, A. Van Schepdael, E. Roets, and J. Hoogmartens (2002) Characterization of polypeptide antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry. J. Pept. Sci. 8: 45-55.   DOI   ScienceOn
12 Gupta, A. K., Y. Kohli, A. Ki, J. Faergemann, and R. C. Summerbell (2000) In vitro susceptibility of the seven Malassezia species to ketoconazole, voriconazol, itaconazol and terbinafine. Br. J. Dermatol. 142: 758-765.   DOI   ScienceOn
13 He, Z., D. Kisla, L. Zhang, C. Yuan, K. B. Green-Church, and A. E. Yousef (2007) Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin. Appl. Environ. Microbiol. 73: 168-178.   DOI   ScienceOn
14 Chung, Y. R., C. H. Kim, I. Hwang, and J. Chun (2000) Paenibacillus koreensis sp. nov. a new species that produces aniturin-like antifungal compound. Int. J. Syst. Evol. Microbiol. 50: 1495-1500.   DOI   ScienceOn
15 Beatty, P. H. and S. E. Jensen (2002) Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotic active against Leptosphaeria maculans, the causative agent of blackleg disease of canola. Can J. Microbiol. 48: 159-169.   DOI   ScienceOn
16 Fortes, T. O., D. S. Alviano, Gl. Tupinamb, T. S. Padro'n, A. R. Antoniolli, C. S. Alviano, and L. Seldin (2008) Production of an antimicrobial substance against Cryptococcus neoformans by Paenibacillus brasilensis Sa3 isolated from the rhizosphere of Kalanchoe brasiliensis. Microbiol. Res. 163: 200-207.   DOI   ScienceOn
17 Kajimura, Y. and M. Kaneda (1997) Fusaricidins B, C and D, new depsipeptide antibiotics produced by Bacillus polymyxa KT-8: isolation, structure elucidation and biological activity. J. Antibiot. 50: 220-228.   DOI   ScienceOn
18 Kurusu, K., K. Ohba, T. Arai, and K. Fukushima (1987) New peptide antibiotics LI-F03, F04, F05, F07, and F08, produced by Bacillus polymyxa. I. Isolation and characterization. J. Antibiot. 40: 1506-1514.   DOI
19 Nakajima, N., S. Chihara, and Y. Koyama (1972) A new antibiotic, Gatavalin I. Isolation and characterization. J. Antibiot. 25: 243-247.   DOI
20 Selim, S., J. Negrel, C. Govaerts, S. Gianinazzi, and D. Van Tuinen (2005) Isolation and partial characterization of antagonistic peptides produced by Paenibacillussp. strain B2 isolated from the sorghum mycorrhizosphere. Appl. Environ. Microbiol. 71: 6501-6507.   DOI   ScienceOn
21 Whipps, J. M. (1987) Effect of media on growth and interactions between a range of soil-born glass-house pathogens and antagonistic fungi. New Phytol. 107: 127-142.   DOI   ScienceOn
22 Ash, C., J. A. E. Farrow, S. Wallbank, and M. D. Collins (1991) Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small subunit-ribosomal RNA sequences. Lett. Appl. microbiol. 13: 202-206.
23 Pfaller, M. A. and W. L. Yu (2001) Antifungal susceptibility testing. New technology and clinical applications. Infect. Dis. Clin. North Am. 15: 1227-1261.   DOI   ScienceOn
24 Choi, H. J., U. H. Yang, Y. E. Kim, Y. H. Choi, C. S. Ahn, Y. K. Jeong, D. W. kim, and W. H. Joo (2010) Antifungal activity of Bacillus sp. BCNU 2003 against the human pathogenic fungi. J. Life Sci. 20: 269-274.   DOI
25 Choi, H. J., C. S. Ahn, Y. K. Jeong, D. W. kim, and W. H. Joo (2010) Antifungal activity of Bacillus sp. BCNU 2002 against the human pathogens. Kor. J. Biotechnol. Bioeng. 25: 123-129.
26 Favre, B., B. Hofbauer, K. S. Hildering, and N. S. Ryder (2003) Comparison of in vitroactivities of 17 antifungal drugs against a panel of 20 dermatophytes by using a microdilution assay. J. Clin. Microbial. 41: 4817-4819.   DOI   ScienceOn
27 Seldin, L., F. Silva de Azevedo, D. S. Alviano, C. S. Alviano, and M. C. de Freire Bastos (1999) Inhibitory activity of Paenibacillus polymyxa SCE2 against human pathogenic micro-organisms. Lett. Appl. Microbiol. 28: 423-427.   DOI   ScienceOn
28 Wang, Z. W. and X. L. Liu (2008) Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using response surface methodology. Bioresource Technol. 99: 8245-8251.   DOI   ScienceOn
29 Jung, J. H. and H. C. Chang (2009) Bacillus polyfermenticus CJ9, Isolated from meju, showing antifungal and antibacterial activities. Kor. J. Microbiol. Biotechnol. 37: 340-349.