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http://dx.doi.org/10.5010/JPB.2002.29.3.185

Somatic Embryogenesis and Plant Regeneration in Mature Seed Cultures and Seed-Derived Embryogenic Suspension Cultures of Yuzu  

Min, Sung-Ran (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Choi, Myung-Suk (Division of Forest Science, Gyeongsang National University)
Jeong, Won-Joong (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Liu, Jang-Ryol (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.29, no.3, 2002 , pp. 185-188 More about this Journal
Abstract
Off-white, friable embryogenic calluses were formed on the internal integument of mature seeds of yuzu (Citrus junos) cultured on Murashige and Skoog's basal medium at a frequency of 1.2%. Embryogenic calluses were proliferated when cultured on medium with 1 mg/L 2,4-D. Upon transfer to medium with 0.1 mg/L kinetin, embryogenic calluses produced numerous somatic embryos. Embryogenic suspension cultures were established by placing embryogenic calluses into liquid medium with 1 mg/L 2,4-D. When plated onto medium with 0.5 mg/L ABA, embryogenic cells developed into somatic embryos at a high frequency, and then regenerated into plantlets. Plantlets were successfully transplanted to potting soil and grown in a greenhouse.
Keywords
Citrus junos; sutaceae; somatic embryo;
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