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http://dx.doi.org/10.3746/jkfn.2009.38.6.801

Purification and Characterization of Transglutaminase from a Newly Isolated Streptomyces platensis YK-2  

Ko, Hee-Sun (Dept. of Microbiology, Keimyung University)
Kim, Hyun-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.6, 2009 , pp. 801-806 More about this Journal
Abstract
A species producing transglutaminase (EC 2.3.2.13) was isolated from forest soil and identified as Streptomyces platensis YK-2. The transglutaminase was purified from culture broth by 50% methanol precipitation, followed by successive chromatography on DEAE-Sephadex. The yield and purification-fold was 63.4% and 2.2-fold, respectively. The purified microbial transglutaminase (MTG) migrated as a single band of approximately 45 kDa upon sodium dodecyl sulfate polyacrylamide gel eletrophoresis. The isoelectric point determined by multichambered electrofocusing was pH $6.0{\sim}7.0$. The enzyme was strongly inhibited by $Hg^{++}$, but was activated by $Cd^{++}$, $Mg^{++}$, $Mn^{++}$, $Pb^{++}$ and reducing agents such as dithiothreitol and mercaptoethanol.
Keywords
microbial transglutaminase; Streptomyces platensis YK-2; purification; methanol precipitation; DEAE-Sephadex;
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