• Food Science and Preservation
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• v.11 no.3
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• pp.411-416
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• 2004

Botanical antimicrobial agent-grapefruit seed extract mixture(BAAG), which could be applied to the preparation of antimicrobial packaging paper, was investigated in order to prove the preservative function of fruits and vegetables. HAAG showed remarkable antimicrobial effects against Fusarium solani Botrytis cinerea, Pencillium crustosum, Erwinia carotovora, Phoma destructiva and Alternaria radicina causing the postharvest decay of fruits and vegetables. We have examined that HAAG could inhibit the growth of microorganims when treated with more than 500 $\mu$g/mL concentration. The activities of HAAG were stable in the wide spectrum of pH and temperature. Direct visualization of microbial cells by using scanning electron microscope showed the loss of microbial cell membrane function, which was destroyed by treating with the dilute solutions of HAAG. We could confirm that HAAG be an antimicrobial agent for the preparation of antimicrobial packaging paper.

• Journal of Mushroom
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• v.5 no.1
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• pp.1-6
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• 2007

A new commercial strain "Hambak" of Grifola frondosa was developed by di-mono crossing between dikaryon of ASI 9031 and monokaryotic strain derived from ASI 9021. It can be cultivated in plastic bottle and bag filled with oak and poplar sawdust substrate which supplemented with 15% rice bran or 10% corn bran. Improvement of cultivation methods was required for shortage of mycelium incubation period and management of humidity in growing room. The optimum temperature of mycelial growth was $25^{\circ}C$ and that of fruiting body development was $15{\sim}18^{\circ}C$. The color of pileus surface was brown and fine pores, whitish to cream-colored, were underside. Primordia formation of Hambak was two days faster than that of Yipsae 1. The yield was $97g/850m{\ell}$ bottle, a bunch of fruiting body was bigger and the quality was better than Yipsae 1.

• Applied Chemistry for Engineering
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• v.16 no.3
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• pp.385-390
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• 2005

In this study, nano sized colloidal Ag was made by using a seed sol method. Colloidal Ag solution was spouted on the surface of the inorganic pigment using the hybridizer system and spray nozzle. Then, the surface of the inorganic pigment was modified by $TiO_2$ to obtain the antibacterial ability. In the manufacturing process of nano sized colloidal Ag, it was confirmed that the size of particles increased by addition of $AgNO_3$ and increased the reaction time. The antibacterial measurement of the inorganic pigment showed that the growth of fungus decreased as the reaction time was increased. After the antibacterial ability appeared, in 5~7 h of the antibacterial inoculation experiment, it was measured that the antibacterial activity was excellent at a fixed time frame. The photodecomposition of benzene using $TiO_2$ as the photocatalyst showed 60~70% efficiency in about 80 min. reaction time. It was shown that more than 90% of this efficiency was achieved in the reaction time of about 30 min.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.291-294
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• 2008

The Streptomyces padanus isolate TH04, isolated from mummified peaches, showed strong antifungal activity to Monilinia fructicola. The inhibition activity of the isolate TH04 to mycelial growth and spore germination at 1% concentration of sub-antifungal powder made from culture suspension (CS) was ranged from 79.8% to 81.0% and from 73.9% to 75.8% to M. fructicola four strains, respectively. In the test of antifungal activity in mixed culture of the isolate and M. fructicola, inhibition rate was 7.5%, 86.8% and 94.0% in 0.01, 0.1, and 1% concentration of CS containing bacterial cell of the isolate, respectively. On apples (cultivar; Fuji), the control values of the isolate TH04 crude filtrates (0.1 and 1%) were 85.9% and 100%, respectively. The results suggest that the isolate TH04 indicate development possibility as biocontrol agent of brown rot caused by M. fructicola with the study on delivery method and fermentation condition to produce an antifungal compound.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.50-50
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• 2018

최근 생태 조경용 관상식물의 수요가 증가함에 따라, 환경적응성이 강하며 관상용으로 가치가 있는 자생식물들을 개발하기 위한 노력이 증가하고 있다. 둥근산꼬리풀(Veronica rotunda Nakai)은 화기가 길며, 꽃이 만개하였을 때 관상가치가 높아 정원의 지피식물 및 실내 분화식물로 이용 가치가 높다. 본 연구는 둥근산꼬리풀의 육묘기술을 개발하기 위하여 2018년 5월 8일에서 6월 30일까지 약 8주간 시행되었다. 본 연구의 공통사항은 200구 트레이에 원예상토를 충진한 후 셀당 4립씩 종자를 파종하였다. 이후 무차광 조건에서 육묘하였으며, 추비는 hyponex $500mg{\cdot}L^{-1}$를 잎이 충분히 젖도록 2, 4주차에 엽면시비 하였다. 파종 용기별 실험은 162, 200, 288구 트레이를 사용하였고, 파종립수별 생육을 알아보기 위하여 200구 트레이에 셀 당 1, 2, 4 및 6립을 파종하였다. 토양종류는 원예상토, 피트모스:펄라이트(3:1, 4:1)의 혼용토를 육묘에 이용하였다. 각 혼용토의 비료 농도는 한아름상토(N:P:K, $300-200-200mg{\cdot}L^{-1}$)를 기준으로 0, 100 및 200%로 조성하였다. 추비는 hyponex (6.5-6-19)를 물에 희석하여 0, 500, 및 $1000mg{\cdot}L^{-1}$로 파종 후 2, 4주차 총 2회 잎이 충분히 젖을 정도로 엽면시비 하였으며, 차광실험은 0, 55 및 75% 차광막이 설치된 곳에 재배하였다. 8주간 육묘 실험을 수행한 결과, 파종 용기별로는 162구 트레이에서 생육한 유묘에서 각 측정 항목별로 유의적으로 양호한 결과가 나타났다. 셀 당 파종립수 별로는 1립 파종구에서 가장 우수한 유묘의 생장을 보였으며, 파종립수가 증가할수록 생육이 감소하는 경향이 나타났다. 토양조성을 달리하였을 때 원예상토 단용 처리에서 가장 생육이 우수하였고, 피트모스가 혼합된 토양에서는 생육이 저조한 경향을 보였다. Hyponex의 농도를 달리하여 추비하였을 때 $1000mg{\cdot}L^{-1}$에서 유묘의 생육이 가장 양호하였으며, 추비농도가 감소할수록 생육이 감소하는 경향을 보였다. 차광정도에 따른 생육을 관찰한 결과 55% 차광에서 생육이 가장 우수하였고, 75% 차광의 경우에는 무차광 처리구에 비해 생육이 감소하는 경향이었다. 따라서 각 처리별 결과를 토대로 둥근산꼬리풀을 육묘시 162구 트레이에 원예상토를 충진한 후 셀 당 1립 파종하여, 55% 차광에서 재배하며, 추비는 Hyponex $1000mg{\cdot}L^{-1}$를 잎이 충분히 젖을 정도로 엽면시비하는 것이 가장 효과적일 것으로 생각된다.

• The Korean Journal of Ecology
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• v.24 no.2
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• pp.93-100
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• 2001

One-year-old cottonwood (Populus deltoides Bartr.) clones, which were classified as sensitive or tolerant, were exposed to 150 n1/1 ozone (O$_3$) over 8 days for 8 hours each day under glass chamber conditions with natural sunlight. The leaves of the sensitive clone had black stipple and bifacial necrosis after $O_3$ treatment. Photosynthesis and stomatal conductance were measured before, during, and after the $O_3$ treatment. The photosynthetic rates due to $O_3$ treatment were decreased 51 percent and 34 percent on the sensitive and tolerant clone, respectively. The stomatal conductance of the sensitive clone was more than 40 percent higher than that of the tolerant clone regardless of the $O_3$ treatment. As light intensity increased, the $O_3$ effect on photosynthesis was clear. Compared to the previous growth chamber studies, our natural light exposure system was able to maintain a stable photosynthetic responses of the control treatment throughout the fumigation period. In addition, changes in assimilation versus intercellular $CO_2$ concentration (A/C curves) showed that $O_3$ decreased the slope and asymptote of the curves for the sensitive clone. This indicates that $O_3$ decreases the biochemical capacity of photosynthesis on the sensitive clone. Chlorophyll contents and fluorescence of the two clones were analyzed to examine the $O_3$ effects on photosystem 11, but $O_3$ did not impact these variables on either clone. Although the tolerant clone did not show any foliar injury, we could not find any ecophysiological defensive responses to $O_3$ treated. Stomatal conductance of the tolerant clone was originally much lower than that of the sensitive one. Thus, the mechanisms of the tolerant clone in this system are to narrowly open stomata and efficiently maintain photosynthesis with a more durable biochemical apparatus of photosynthesis under $O_3$ stress. The sensitive clone has higher photosynthetic capacity and more efficient light reaction activity than the tolerant one under charcoal filtered condition, but is not as resilient under stress.

• Research in Plant Disease
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• v.20 no.2
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• pp.112-118
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• 2014

A liquid bio-formulation containing chitinolytic bacteria, chitin and their products was assessed for its potential biological control against root-knot nematodes on cucumber. The bio-formulation was prepared by cultures of three chitinolytic bacteria, Chromobacterium sp. strain C-61, Lysobacter engymogenes and Serratia plymuthica in minimal medium supplemented with chitin. Under pot conditions, the bio-formulation showed better growth of cucumber plants, and less root galls and population density of Meloidogyne spp. than control media without the bio-formulation. In a greenhouse, 75-fold diluted bio-formulations were treated instead of water around cucumber plants through hoses for drip irrigation six times at 5-day intervals from the transplanting date. After 30 and 60 days, the treatment provided about 7% and 10% enhancement in the plant height and about 78% and 69% reduction in population density of Meloidogyne spp. in the rhizosphere, respectively. In addition, the experiments showed that the control effects occurred only in the soils contacted with the bio-formulation. Undiluted bio-formulations were drenched three times at 10-day intervals around cucumber plants severely infested with Meloidogyne spp. The treatment showed about 37% plant enhancement without dead plants compared with 37% death in the untreated control, and about 82% nematode reduction. These results suggest that the bio-formulation can be practically used to control the root-knot nematode on cucumber.

• Korean Journal of Environmental Biology
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• v.28 no.1
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• pp.30-39
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• 2010

We researched community structure, species composition, and habitat environment of Dendropanax morbifera populations distributed in Goheung, Haenam, Wando (I.), and Bogildo (I.) at south coast of the Korean Peninsula. Our research was also estimated possible area for growth of Dendropanax morbifera by analyzing meteorological factors of the habitat thereof. Dendropanax morbifera community divided into Castanopsis cuspidata var. sieboldii group and Carpinus laxiflora group by difference of species composition. Broad-leaved evergreens showed high dominance value in view of rNCD in Dendropanax morbifera community as follows: Camellia japonica (100.0), Quercus acuta (88.6), Castanopsis cuspidata var. sieboldii (55.8), Dendropanax morbifera Lev. (41.4), Trachelospermum asiaticum var. intermedium (23.8), Eurya japonica (19.9) and Ligustrum japonicum (11.5). In the population distribution, 94.2% of Carpinus laxiflora group consisted of sapling and small size class that are less than 5 cm in DBH while 54.2% of Castanopsis cuspidata var. sieboldii group did. The soil environment of habitat showed low soil acidity, good organic matter and water content, and good drainage. The distribution area of Dendropanax morifera habitat had $13^{\circ}C$ or more in annual mean temperature, $7.4^{\circ}C$ or more in daily minimum temperature, $100^{\circ}C$ month or more in warmth index, and approximately 1,344 mm in mean annual precipitation. Dendropanax morifera habitat corresponded to evergreen broad-leaved forest zone located in islands and coast in the southwest region of the Korean Peninsula. The northern limit line of Dendropanax morifera community was determined as Gunsan-Jeongeup-Gwangju-Jinju-Pohang-Youngdeok line based on $13^{\circ}C$ in annual mean temperature that was confirmed in natural habitat of Dendropanax morifera community.

• Korean Journal of Environmental Biology
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• v.25 no.1
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• pp.56-65
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• 2007

The aim of this study is to provide a fundamental data which can be applied to management conservation, and restoration of coastal sand dune vegetation by determining the classification and distribution of community, and ecological characteristics of the habitat. This research was performed by the Braun-Blanquet's method. The coastal sand dune vegetation of Uido (I.) was composed with 9 communities as follows : Vitex rotundifolia community, Zoysia macrostachya community, Carex kobomugi community, Iachaemum anthephoroides community, Imperata cylindrica var. koenigii community, Carex pumila community, Calystegia soldanella community Messerschmidia sibirica community and Pinus thunbergii community, the evergreen needle-leaved forest. The constancy degree showed high in Calystegia soldanella (77%), Vitex rotundifolia (74%), Carex kobomugi (66%), Zoysia macrostachya (50%) and Imperata cylindrica var. koenigii (47%). However the highest constancy degree Calystegia soldanella has, it turned out to have low net contribution degree (NCD) in each community. In terms of the distribution and growth feature of the composition species in the coastal sand dune, Vitex rotundifolia, Carex kobomuri and Zoysia macrostachya were widely distributed from unstable sand dune to stable one but Iachaemum anthephoroides and Imperata cylindrica var. koenigii were mainly found at the stable sand dune. Carex kobomugi was especially dominant at the unstable sand dune where the sand continued to be deposited. On the other hand, Carer pumila and Messerschmidia sibirica showed regional distributions around fresh water.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.4
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• pp.235-238
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• 2008

The Water Erosion Prediction Project (WEPP) was initiated in August 1985 to develop new generation water erosion prediction technology for federal agencies involved in soil and water conservation and environmental planning and assessment. Developed by USDA-ARS as a replacement for empirical erosion prediction technologies, the WEPP model simulates many of the physical processes important in soil erosion, including infiltration, runoff, raindrop detachment, flow detachment, sediment transport, deposition, plant growth and residue decomposition. The WEPP included an extensive field experimental program conducted on cropland, rangeland, and disturbed forest sites to obtain data required to parameterize and test the model. A large team effort at numerous research locations, ARS laboratories, and cooperating land-grant universities was needed to develop this state-of-the-art simulation model. The WEPP model is used for hillslope applications or on small watersheds. Because it is physically based, the model has been successfully used in the evaluation of important natural resources issues throughout the United State and in several other countries. Recent model enhancements include a graphical Windows interface and integration of WEPP with GIS software. A combined wind and water erosion prediction system with easily accessible databases and a common interface is planned for the future.