농약과학회지 (The Korean Journal of Pesticide Science)

  • 제12권3호
  • /
  • Pages.291-294
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  • 2008
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지

잿빛무늬병균에 대한 Streptomyces padanus isolate TH04의 항균활성

Antifungal Activity of Streptomyces pad anus isolate TH04 against Monilinia fructicola, Brown rot Fungus on Stone-fruits

  • 임태헌 (삼호유비 농생명과학연구소) ;
  • 최용화 (상주대학교 식물자원학과) ;
  • 이동운 (상주대학교 생물응용학과) ;
  • 한상섭 (전북대학교 산림자원학과) ;
  • 차병진 (충북대학교 식물의학과)
  • Lim, Tae-Heon (Research Institute of Agri-Bio Science, Samhoub, Co., Ltd.) ;
  • Choi, Yong-Hwa (Department of Applied Biology, Kyungpook National University) ;
  • Lee, Dong-Woon (Department of Plant Resources, Kyungpook National University) ;
  • Han, Sang-Sub (Department of Forest Resources, Chonbuk National University) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
  • 발행 : 2008.09.30
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초록

복숭아 미이라 과일로부터 복숭아 잿빛무늬병 Monilinia fructicola에 대하여 강한 항균활성을 보이는 방선균 Streptomyces padanus TH04를 분리하였다. TH04 균주의 배양 추출물 1%를 함유한 배지에서의 균사생육 및 포자발아는 시험한 M. fructicola의 strain에 따라 각각 $79.8{\sim}81%$와 $73.9{\sim}75.8%$ 억제되었다. 병원균과 TH04 균주 초기접종 밀도를 0.01%, 0.1% 및 1%로 달리하여 동시배양 한 결과, 항균활성은 선발 방선균의 밀도에 따라 $7.5%{\sim}94%$로 나타났다. 사과(품종; 후지)를 이용한 조추출물의 항균활성은 0.1% 처리구 85.9%, 1%처리구 100%로 나타났다. 항균활성 물질 생산, 안정성 및 제형화에 관한 연구가 이루어질 경우 선발한 Streptomyces padanus TH04는 생물학적 방제제로의 개발 가능성 있을 것으로 생각된다.

The Streptomyces padanus isolate TH04, isolated from mummified peaches, showed strong antifungal activity to Monilinia fructicola. The inhibition activity of the isolate TH04 to mycelial growth and spore germination at 1% concentration of sub-antifungal powder made from culture suspension (CS) was ranged from 79.8% to 81.0% and from 73.9% to 75.8% to M. fructicola four strains, respectively. In the test of antifungal activity in mixed culture of the isolate and M. fructicola, inhibition rate was 7.5%, 86.8% and 94.0% in 0.01, 0.1, and 1% concentration of CS containing bacterial cell of the isolate, respectively. On apples (cultivar; Fuji), the control values of the isolate TH04 crude filtrates (0.1 and 1%) were 85.9% and 100%, respectively. The results suggest that the isolate TH04 indicate development possibility as biocontrol agent of brown rot caused by M. fructicola with the study on delivery method and fermentation condition to produce an antifungal compound.

키워드

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