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http://dx.doi.org/10.5423/RPD.2014.20.2.112

Control of the Root-Knot Nematode (Meloidogyne spp.) on Cucumber by a Liquid Bio-Formulation Containing Chitinolytic Bacteria, Chitin and Their Products  

Ha, Woo Jong (Department of Plant Biotechnology and Biotechnology Research Institute, Chonnam National University)
Kim, Young Cheol (Department of Plant Biotechnology and Biotechnology Research Institute, Chonnam National University)
Jung, Hyuncha (Department of Forest Resources, Sunchon National University)
Park, Seur Kee (Department of Agricultural Biology, Sunchon National University)
Publication Information
Research in Plant Disease / v.20, no.2, 2014 , pp. 112-118 More about this Journal
Abstract
A liquid bio-formulation containing chitinolytic bacteria, chitin and their products was assessed for its potential biological control against root-knot nematodes on cucumber. The bio-formulation was prepared by cultures of three chitinolytic bacteria, Chromobacterium sp. strain C-61, Lysobacter engymogenes and Serratia plymuthica in minimal medium supplemented with chitin. Under pot conditions, the bio-formulation showed better growth of cucumber plants, and less root galls and population density of Meloidogyne spp. than control media without the bio-formulation. In a greenhouse, 75-fold diluted bio-formulations were treated instead of water around cucumber plants through hoses for drip irrigation six times at 5-day intervals from the transplanting date. After 30 and 60 days, the treatment provided about 7% and 10% enhancement in the plant height and about 78% and 69% reduction in population density of Meloidogyne spp. in the rhizosphere, respectively. In addition, the experiments showed that the control effects occurred only in the soils contacted with the bio-formulation. Undiluted bio-formulations were drenched three times at 10-day intervals around cucumber plants severely infested with Meloidogyne spp. The treatment showed about 37% plant enhancement without dead plants compared with 37% death in the untreated control, and about 82% nematode reduction. These results suggest that the bio-formulation can be practically used to control the root-knot nematode on cucumber.
Keywords
Bio-formulation; Chitinolytic bacteria; Cucumber; Root-knot nematode;
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