• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.3 s.258
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• pp.380-387
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• 2007

We carried out an image analysis of living cells forming their contacts at the bottom of the cell culturing substrate. In order to visualize the contact area selectively, we adopted total-internal-reflection-fluorescence (TIRF) method, which can illuminate the specimen volume within only several hundred nano-meters above the substrate. From the fluorescent intensity of the TRF image, we could calculate the distance of the cell surface from the substrate. As a result, we visualized the origin of cell contacts, their movements, and the change of cell-contact type from the close-contact into focal-contact with information of its vertical displacement representing the temporal evolution process of the three-dimensional cell-surface-profile near the contact area during this metamorphosis.

• Journal of Sensor Science and Technology
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• v.19 no.2
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• pp.130-136
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• 2010

The microbolometer array sensor with fine pitch pixel array has been implemented to the released amorphous silicon layer supported by two contact pads. For the design of focal plane mirror with geometrical flatness, the simple beam test structures were fabricated and characterized. As the beam length decreased, the effect of beam width on the bending was minimized, Mirror deformation of focal plane in a real pixel showed downward curvature by residual stress of a-Si and Ti layer. The mirror tilting was caused by the mis-align effect of contact pad and confirmed by FEA simulation results. The properties of bolometer have been measured as such that the NETD 145 mK, the TCR -2 %/K, and thermal time constant 1.99 ms.

• Journal of the Korean Society of Visualization
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• v.8 no.3
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• pp.22-28
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• 2010

In the present study, we performed the velocity field measurement in a microchannel using a focal length variable micro liquid lens. The liquid lens is used as a beam expander in a micro-PIV system to acquire the scatter image of the seeded particle. A thin film-type micro liquid lens was made of PDMS material and it was attached on top of the 700-micron-wide working fluid supply channel trench. As a result, the focal length and contact angle of the liquid lens changed with variations in applied pressure.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1455-1457
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• 2008

Cell adhesion is a coordinated process involving initial binding of integrin receptors to extracellular matrix (ECM), recruitment of adhesion proteins, and focal adhesion assembly. The formation of mechanically stable focal adhesion assembly of cells within surrounding ECM is a key parameter to direct numerous cellular functions including cell migration, differentiation, and apotosis. With current cell adhesion assays, it is difficult to understand contributions of each coordinated event on evolution of cell adhesion strengthening since cells spontaneously spread upon their adhesion to the substrate, thus remodeling their cytoskeletal structure. In this presentation, novel approaches for analysis of cell adhesion strengthening process based on the combination of mechanical device, micro-patterned substrates, and molecular biological techniques will be discussed.

• Proceedings of the KSME Conference
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• 2005.11a
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• pp.69.1-69.1
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• 2005

• Journal of the Korean institute of surface engineering
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• v.42 no.5
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• pp.203-207
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• 2009

Interaction between human osteoblast (hFOB 1.19) and CrN films was conducted in vitro. CrN films were produced by cathodic arc plasma deposition. The surface was characterized by atomic force microscopy (AFM). CrN films, glass substrates and TiN films were cultured with human osteoblasts for 48 and 72 hours. Actin stress fiber patterns and cell adhesion of osteoblasts were found less organized and weak on CrN films compared to those on the glass substrates and the TiN films. Human osteoblasts also showed less proliferation and less distributed microtubule on CrN films compared to those on glass substrates and TiN films. Focal contact adhesion was not observed in the cells cultured on CrN films, whereas focal contact adhesion was observed well in the cells cultured on glass substrates and TiN films. As a result, the CrN film is a potential candidate as a surface coating to be used for implantable devices which requires minimal cellular adhesion.

• Journal of the Korean Society for Nondestructive Testing
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• v.25 no.2
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• pp.74-80
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• 2005

A new dimension measuring method for the measurement of diameter of an object has been developed using laser triangulation. The 3D data of an object was calculated from the 2 dimensional image information obtained by the laser stripe using the laser triangulation. The system that use existing theory can measure the diameter of hole not only in a normal plane but also ill an incline plane. However, in the existing theory, since the lens with fixed feral length was used, the area of measurement was fixed. The simplest way to solve this problem is to change distance between a CCD camera and object. Other way is to use a zoom lens having variable focal length. In this paper, the zoom lens with variable focal length was used. Therefore, we ran experiment with magnification that is optimized according to size of object using zoom lens with variable focal length.

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1629-1635
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• 2012

Previously, we demonstrated that the erythropoietin receptor (EpoR) is present on fibroblasts, where it regulates focal contact. Here, we assessed whether this action of EpoR is involved in the reduced cell adhesion observed in colonocytes exposed to Clostridium difficile toxin A. EpoR was present and functionally active in cells of the human colonic epithelial cell line HT29 and epithelial cells of human colon tissues. Toxin A significantly decreased activating phosphorylations of EpoR and its downstream signaling molecules JAK-2 (Janus kinase 2) and STAT5 (signal transducer and activator of transcription 5). In vitro kinase assays confirmed that toxin A inhibited JAK 2 kinase activity. Pharmacological inhibition of JAK2 (with AG490) abrogated activating phosphorylations of EpoR and also decreased focal contacts in association with inactivation of paxillin, an essential focal adhesion molecule. In addition, AG490 treatment significantly decreased expression of occludin (a tight junction molecule) and tight junction levels. Taken together, these data suggest that inhibition of JAK2 by toxin A in colonocytes causes inactivation of EpoR, thereby enhancing the inhibition of focal contact formation and loss of tight junctions known to be associated with the enzymatic activity of toxin A.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.1
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• pp.31-37
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• 2007

A $^{192}Ir$ small-focal source has been developed by using the HANARO reactor and the radioisotope production facility at the Korea Atomic Energy Research Institute (KAERI). The small-focal source with the dimension of 0.5 mm in diameter and 0.5 mm in length was fabricated as an aluminum-encapsulated form by a specially designed pressing equipment. For the estimation of the radioactivity, neutron self-shielding and ${\gamma}-ray$ self-absorption effects on the measured activity was considered. From this estimation, it is realized that $^{192}Ir$ small-focal sources over 3 Ci activities can be produced from the HANARO. Field performance tests were performed by using a conventional source and the developed source to take images of a computer CPU and a piece of a carbon steel. The small-focal source showed better penetration sensitivity and geometrical sharpness than the conventional source does. It is concluded from the tests that the focal dimension of this source is small enough to maximize geometrical sharpness in the image taking for the close proximity shots, pipeline crawler applications and contact radiography.

• Proceedings of the Korean Society of Laser Processing Conference
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• 2005.06a
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• pp.3-8
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• 2005

The robot systems are widely used in the welding manufacturing. The essential tasks to operate the welding robot are the acquisition of the position and/or shape of the parent metal. For the seam tracking or the robot automation, many kinds of contact and non-contact of the system which monitors the shape of the welding part is described. This system uses the line-type structured laser diode and the visual sensor. It includes the correction of radial distortion which is often found in the image from the camera with short focal length. Direct Linear Transformation (DLT) is used for the camera calibration. The three dimensional shape of the parent metal is obtained after simple linear transformation. Therefore, the system operates in real time. Some experiments are carried out to evaluate the performance of the developed system.