Transactions of the Korean Society of Mechanical Engineers A (대한기계학회논문집A)

The Korean Society of Mechanical Engineers (대한기계학회)
권호 표지
DOI QR코드

DOI QR Code

On the Dynamic Characteristics of Cell Contact by Analyzing TIRE Images

전반사 형광 이미지 분석을 통한 세포 부착점의 운동 특성에 관한 연구

  • Published : 2007.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

We carried out an image analysis of living cells forming their contacts at the bottom of the cell culturing substrate. In order to visualize the contact area selectively, we adopted total-internal-reflection-fluorescence (TIRF) method, which can illuminate the specimen volume within only several hundred nano-meters above the substrate. From the fluorescent intensity of the TRF image, we could calculate the distance of the cell surface from the substrate. As a result, we visualized the origin of cell contacts, their movements, and the change of cell-contact type from the close-contact into focal-contact with information of its vertical displacement representing the temporal evolution process of the three-dimensional cell-surface-profile near the contact area during this metamorphosis.

Keywords

References

  1. Cooper, J. M. and Cass, A. E. G., 2002, 'Miniaturized Screening Technologies for Drug Discovery,' Biochem. Soc. Trans., Vol. 30, part 4, pp. 802-806 https://doi.org/10.1042/BST0300802
  2. Newgreen, D. F., 1989, 'Physical Influences on Neural Crest Cell Migration in Avian Embryos: Contact Guidance and Spatial Restriction,' Int. J. Dev. Biol., Vol. 131, No. 1, pp. 136-148
  3. Zygourakis, K., 1996, 'Quantification and Regulation of Cell Migration,' Tissue Eng., Vol. 2, No. 1, pp. 1-26 https://doi.org/10.1089/ten.1996.2.1
  4. Gingell, D., Heavens, O. S. and Mellor, J. S., 1987, 'General Electromagnetic Theory of Total Internal Reflection Fluorescence: the Quantitative Basis for Mapping Cell-substratum Topography,' J. Cell Sci., Vol. 87, No. 5, pp. 677-693
  5. Burmeister, J. S., Olivier, L. A., Reichert, W. M. and Truskey, G. A., 1998, 'Application of Total Internal Reflection Fluorescence Microscopy to Study Cell Adhesion to Biomaterials,' Biomaterials, Vol. 19, pp. 307-325 https://doi.org/10.1016/S0142-9612(97)00109-9
  6. Axelrod, D., 1981, 'Cell-substrate Contacts Illuminated by Total Internal Reflection Fluorescence,' J. Cell Biol., Vol. 89, pp. 141-145 https://doi.org/10.1083/jcb.89.1.141
  7. Burridge, K. and Fath, K., 1989, 'Focal Contacts: Transmembrane Links Between the Extracellular Matrix and the Cytoskeleton,' BioEssays, Vol. 10, No. 4, pp. 104-108 https://doi.org/10.1002/bies.950100403
  8. Webb, D. J., Brown, C. M. and Horwitz, A. F., 2003, 'Illuminating Adhesion Complexes in Migrating Cells; Moving Toward a Bright Future,' Current Opinion in Cell Biol., Vol. 15, pp. 614-620 https://doi.org/10.1016/S0955-0674(03)00105-4
  9. Izzard, C. S. and Lochner, L. R., 1980, 'Formation of Cell-to-substrate Contacts During Fibroblast Motility: an Interference-reflexion Study,' J. Cell Sci., Vol. 42, pp. 81-116
  10. Toomre, D. and Manstein, D. J., 2001, 'Lighting up the Cell Surface with Evanescent Wave Microscopy,' Trends in Cell Biol., Vol. 11, No. 7, pp. 298-303 https://doi.org/10.1016/S0962-8924(01)02027-X
  11. Smilenov, L. B., Mikhailov, A., Pelham Jr., R. J., Marcantonio, E. E. and Gundersen, G. G., 1999, 'Focal adhesion motility revealed in stationary fibroblasts,' Science. Vol. 286, pp.1172-1174 https://doi.org/10.1126/science.286.5442.1172

Cited by

  1. Review — Transport phenomena associated with cells incurring diseases vol.21, pp.11, 2007, https://doi.org/10.1007/BF03177441