• Horticultural Science & Technology
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• v.30 no.2
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• pp.162-170
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• 2012

The objectives of this study were to isolate and the sequence of novel $F3'H$ gene related to an anthocyanin pathway, and to confirm the expression patterns of the gene involved in the flower color variations of chrysanthemum mutants. In this study, we isolated the full-length cDNAs and the genomic DNAs of an $F3'H$ gene from a wild type (WT) chrysanthemum (cv. Argus) and its three color mutants. The sequence analysis revealed a putative open reading frame of 1,527 bp that encodes a polypeptide of 509 amino acids. Sequence homology ranged from 97% to 99% between 'Argus' and its three color mutants. The sequence analysis from the genomic DNA revealed that the chrysanthemum $DgF3'H$ gene consisted of three exons and two introns spanning a 3,830 bp length. The sizes of the gene for three mutants ranged from a shorter size of 3,828 bp to a longer size of 3,838 bp when compared to the size of WT. The total size of the two introns was 2,157 bp for WT, but those for three color mutants ranged from 2,154 bp to 2,159 bp. A result of an RT-PCR analysis indicated that the color variations of the mutants AM1 and AM2 can be partly explained by the structural modification derived from the sequencial changes in the gene caused by gamma ray. A Southern blot analysis revealed that the $DgF3'H$ gene existing as multiple copies in the chrysanthemum genome. A systemic study will be further needed to provide a genetic mechanism responsible for the color mutation and to uncover any involvement of genetic elements for the expression of the $DgF3'H$ gene for the color variation in chrysanthemum.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.191-197
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• 1984

This study was conducted to investigate the flowering habit for the botanical types of peanut (Arachis hypogaea. L) in cultural limiting region. Eight peanut varieties pertaining to virginia, Spanish, Valencia, Shinpung type were used in this study. Shinpung type, a new erect type was developed at Crop Experiment Station, ORD. Appearance of the first flower of peanut plants is observed at a relatively early growing stage. Number of days to first flower were 41 days under vinyl-mulching while non-mulching culture took 55 days. Number of days to first flower were delayed in order of Valencia, Spanish, Shinpung, Virginia type. Flowering durations were distinguished among botanical types and days of those were Virginia 85-93, Spanish 101-105, Valencia 106-113, Shinpung 82-88 days, respectively. The Spanish and Valencia types produced more flowers than Virginia and Shinpung types. The maximum flowering stage of Shinpung and Virginia types reached early and ceased by one cycle, while Spanish and Valencia types came late and showed maximum stage through the early and late stage during the growth stage. By vinyl-mulching, the maximum flowering stage was shortened by 20 days than that of non-mulching. Distribution of flowers for each of branch positions to total flowers occupied 52-53% in two cotyledonary branchs, 23-27% in 3rd to 5th branchs, 20-25% in over. 6th branchs. Shinpung type was considered more favorable botanical type than the other types for the breeding of early maturing high yielding variety because of shortening of days to first flower, flowering duration and distribution of lower branch of effective flowers.

• Korean Journal of Plant Resources
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• v.26 no.2
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• pp.284-288
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• 2013

This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. The optimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploid plant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, the higher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. While individuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and no tetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulum Thunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05% for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.443-447
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• 2015

Fragaria ${\times}$ ananassa (Duch.) cultivars, 'Wongyo 3 115' a nd 'Wongyo 3 116', we re bre d as a n inte rme diate parent l ine using heterosis of $F_1$ hybrids in octoploid strawberry. These two new cultivars were developed as inbred lines ($S_9$ generation) propagated from both seed and runners/stolons. 'Wongyo 3115', an inbred line derived from the 'Benihoppe' cultivar, has a semi-upright plant shape showing a vigorless growth habit, weaker than the original 'Benihoppe' cultivar; it has early flower bud differentiation and produces 10 flowers per cluster when grown from healthy seed. 'Wongyo 3115' has low yields of conical type fruit with pink colored skin; average fruit weight is approximately 11 g. The important characteristic of 'Wongyo 3115' is its excellent fruit firmness, firmer than the original variety. 'Wongyo 3116', an inbred line derived from the 'Doyonoka' cultivar, has an upright plant shape, vigorless growth habit, weaker than that of the original 'Doyonoka' cultivar; it has early flower bud differentiation and produces 8 flowers per cluster when grown from healthy seed. 'Wongyo 3 116' has oblate type fruits with red skin and an ave rage weight of approximately 12 g. The important characteristics of 'Wongyo 3116' are excellent fruit shape and higher yield than other inbred lines, although it produces small fruits compared to the original cultivar. Both cultivars have excellent taste, high sugar/acid ratio, and good texture. In regards to disease and pest resistance, 'Wongyo 3115' and 'Wongyo 3116' have a tendency to be sensitive to powdery mildew, anthracnose and the two-spotted spider mite.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.292-297
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• 2015

A new cultivar of Hibiscus 'Red Bohanjae' (Hibiscus syriacus 'Red Bohanjae') was developed after crossing between Hibiscus syriacus 'Andong' (♀) with white flowers and Hibiscus syriacus 'Samchully' (♂) with pink flowers to improve flower quality and growth habit. The Hibiscus breeding program was initiated in 2002, and H. syriacus 'Red Bohanjae' was preliminarily selected as '02-RV-17' in 2003 due to its stable flower quality with rich pink flowers and small tree type. The selected line was further evaluated for growth characteristics, leaf shape, leaf size, flowering characteristics, and tested for distinctness, homogeneity, and stability during 2004-2007. The characteristics preserved by grafting. 'Red Bohanjae' showed a small growth habit with slightly upright branches. The tree had potential use as a potted plant or bonsai because of its dwarf form. The new cultivar 'Red Bohanjae' had violet-pink color flowers (RHS N78C) with red eye spots, small size (9.2 cm) and spatulate (I-type) petals. Leaves were 4.2 cm long and 2.3 cm wide. After the evaluation of plant characteristics for 3 years (2005-2007), it was registered as cultivar 'Red Bohanjae' (4118, plant variety protection rights number) in 2012. 'Red Bohanjae' having violet-pink flowers with red eye and dwarf tree form seems to be suitable for pot or bonsai cultivation in both indoor and outdoor conditions.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.618-623
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• 2015

The new white calla lily (Zantedeschia aethiopica) cultivar 'White Cutie' was bred at the National Institute of Horticultural & Herbal Science (NIHHS) in 2011. 'Childsiana' showing the multi-flowering characteristic and 'Wedding March' resistant to soft rot disease were artificially crossed in 2004. Of the progeny, 'White Cutie' was selected specifically for use in cut flower production after investigation over seven years (2005 to 2011) of genetic and phenotypic characteristics, resistance against soft rot, and customer preference regarding the culture vigor and post-harvest quality. 'White Cutie' was early flowering (85.6 days to flowering) with white flowers (RHS W155C), although it had a mid-sized flower in which spathe height and width were 8.6 cm and 8.7 cm, respectively. It was multi-flowering (6.2 flowers per plant) and produced a very high number of cormels (13.4 per plant). Furthermore, it was resistant to soft rot disease.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.573-578
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• 2012

A total of eleven Chinese cabbage accessions were used for microspore culture and were grown to take basal data. Based on the collected data, breeding materials were chosen to develop new improved Chinese cabbage cultivars. The range of microspore-derived embryoid taken from flower buds was 1.6 to 35.4 embryoids. The embryoids from IT26110 and IT26153 among the Chinese cabbages were more than 34 per flower bud. The viability rate after cold treatment was low from 0.2 to 11.7%. The range of fertility rate was 7.7 to 58.8% in general but the IT26118, IT26122, IT26128, IT26130, and IT26164 were more than 50%. The result of their seed production ability by selfing was 11.9 seeds per siliqua in IT26128 while the others were less than 10 seeds. In the microspore culture using parents of different hereditary, the number of embryoids, the number of plants, the rate of fertility and their pure seed production ability appeared to be very different in doubled haploid lines obtained from fertile plants of Chinese cabbage.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.224-230
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• 2013

Lily is an important cut-flower in Korea and world as well due to it's a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with $150mg{\cdot}l^{-1}$ of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with $100mg{\cdot}l^{-1}$ of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, $150mg{\cdot}l^{-1}$ of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.1
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• pp.133-138
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• 1983

In Korea, Brassica species planted in summer remain in rosette and do not flower. Seed production of vernalized rape seedlings grown in summer was evaluated to establish method of generation shortening and genetically pure seed production without isolation of seed fields. When vernalized rape seedlings of different maturity groups were transplanted in summer, all plants bolted and flowered, and average days from sowing to bolting and flowering was 37 and 58. respectively. It took 85days for early maturity group, 88 days for medium maturity group, and 108 days for late maturity group to mature. Seed yield of vernalized plants grown in summer was 8.8 gr per plant and 53kg per l0a which was 23% of seed yield of rape planted in autumn. However, enough seeds were produced to test oil composition and cake and to plant for a next generation. If seedlings are raised, they can be transplanted in more than 1, 000 times of original seed field, indicating that genetically pure seed can be produced without caging seed fields with nets and isolation of seed fields since other Brassica species do not flower in summer.

• Journal of Korean Society of Forest Science
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• v.59 no.1
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• pp.31-36
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• 1983

Flowering time of Pinus rigida Mill and Pinus taeda L, in an $F_1$-hybrid seed orchard was investigated for five years from 1971 through 1975. The two tree species flowered during late April to early May at the observation site, Flowering patterns of the two species of different sex of the same species changed yearly during the five observation years. Floral development of the two species appeared to depend largely on temperature factor during the period of the initiation of floral organs up to flowering provided that other environmental factors are normal. Six-days difference in flowing time between female inflorescence of pitch (flower later) and male inflorescence of loblolly (flower earlier) pines effectively isolated the two species reproductively on population levels. Not all of selected trees of the two species for their synchronized flowering appeared to be useful as parental trees for the establishment of $F_1$-hybrid seed orchards. With the result from this investigation the author suggested to use a modified simple recurrent selection method for pitch-loblolly hybrid pine breeding.