Browse > Article
http://dx.doi.org/10.7732/kjpr.2013.26.2.284

A Tetraploid Induction in Hypericum patulum Thunberg by Colchicine Soaking Treatment  

Kwon, Soo Jeong (Department of Flower Floral Plant Coordi & Landscape Architecture, Woosong College)
Cho, Kab Yeon (Department of Food Nutrition and Cookery, Woosong College)
Kim, Hag Hyun (Department of Flower Floral Plant Coordi & Landscape Architecture, Woosong College)
Publication Information
Korean Journal of Plant Resources / v.26, no.2, 2013 , pp. 284-288 More about this Journal
Abstract
This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. The optimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploid plant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, the higher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. While individuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and no tetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulum Thunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05% for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid.
Keywords
DNA content; Explant; Flow cytometry; Number of chloroplasts; Soaking treatment;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kwon, S.J., U.D. Shin, I.H. Kim and H.H. Kim. 2009. Tetraploid induction in Prunella vulgaris for. albiflora by colchicine soaking treatment. Kor. J. Hort. Sci. Technol. 27 (Suppl. I):151 (in Korean).
2 Lapins, K.O. 1975. Polyploidy and mutations induced in apricot by colchicine treatment. Can. J. Genet .Cytol. 17:591-599.   DOI
3 Miyoshi, K. and N. Asakura. 1996. Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gevera jamesonii). Plant Cell Rep. 16:1-5.   DOI   ScienceOn
4 Park, K.J. 1994. Cold-hardiness and tetraploid induced by colchicine treatment in mulberry seedlings(Morus alba L. Yongchonppong/Kaeryanppong). Korean J. Seric. Sci. 36(1): 1-7 (in Korean).   과학기술학회마을
5 Sari, N., K. Abak and M. Pitrat. 1999. Comparison of ploid level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. & Nakai. Sci. Hort. 82:265-277.   DOI   ScienceOn
6 Bae, C.H., Y.H. Lee, D.C. Yang, K.S. Min, H.I. Kim and H.Y. Lee. 2001. Changes of chloroplast number per guard cell pairs of leaves by ploidy level in Nicotiana tabacum L. cv. BY-4. Korean J. Plant Tissue Cult. 28(4):179-184 (in Korean).   과학기술학회마을
7 Bamberg, J.B. and R.E. Hanneman Jr. 1991. Rapid ploid screening of tuber bearing Solanum (potato) species through pollen diameter measurement. Am. Potato J. 68:279-285.   DOI
8 Bingham, E.T. 1969. Haploids from cultivated alfalfa Medicago sativa L. Nature 221:865-866.   DOI   ScienceOn
9 Borrino, E.M. and W. Powell. 1987. Stomatal guard cell length as an indicator of ploid in microspore derived plants of barley. Genome 30:158-160.
10 Chaudhari, H.K. and J.R. Barrow. 1975. Identification of cotton haploids by stomatal chloroplast-count technique. Crop Sci. 15:760-763.   DOI
11 Cho, H.M., H.Y. Kim and I.G. Mok. 1994. Stomatal cell characters as an indicator for haploid indentification from progeny plants derived by 4x${\times}$2x interspecific crosses in potatoes. Korean J. Breed 26(1):66-73 (in Korean).
12 Cockerham, L.E. and G.J. Galletta. 1976. A survey of pollen characteristics in certain Vaccinium species. J. Amer. Soc. Hort. Sci. 101:671-676.
13 Dudley, J.W. 1968. Number of chloroplasts in guard cells of inbred lines of tetraploid and diploid sugarbeets. Agron. J. 50(3):169-170.
14 Hermsen, J.G.TH., and J. Verdenius. 1973. Selection from Solanum tuberosum group 'Phureja' of genotypes combining high-frequency haploid induction with homozygosity for embryo-spot. Euphytica 22:244-259.   DOI
15 Galbraith, D.W., K.R. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma and E. Firoozabady. 1983. A rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049-1051.   DOI   ScienceOn
16 Hadlaczky, G., G. Bistrary, T. Parznovszky and D. Dudits. 1983. Mass isolation of plant chromosomes and nuclei. Planta 157:278-385.   DOI   ScienceOn
17 Hahn, S.J. 1969. Studies on the tetraploid radish (Raphanum sativus L.) induced by colchicine. On the resistance the virus diseases. J. Kor. Soc. Hort. Sci. 5:48-56 (in Korean).
18 Hougas, R.W. and S.J. Peloquin. 1957. A haploid plant of the potato variety 'Katahdin'. Nature 180:1209-1210.   DOI
19 Kim, H.H. 1999. Studies on establishment of cultivation system and breeding of Codonopsis lanceolata (Sieb. et Zucc.) Trautv. PhD Diss., Kyushu Univ. (in Japanese).
20 Kim, I.H., H.H. Kim, E.Y. Hong, J.S. Yun, T. Yun, J.K. Hwang and C.H. Lee. 2003a. Breeding of tetraploid in Platycodon grandiflorum (Jacq.) A. DC. by colchicine treatment. Korean J. Plant Res. 6(3):188-194.   과학기술학회마을
21 Kim, I.H. 2003b. Breeding of tetraploid in Codonopsis lanceolata (Sieb. et Zucc.) Trautvetter by colchicine treatment. Korean J. Plant Res. 6(3):227-232.   과학기술학회마을
22 Kwon, S.J., J.H. Park, S.H. Woo and H.H. Kim. 2012. The effective approach to induce tetraploid plant of Prunella vulgaris for. albiflora by colchicine soaking treatment. Korean J. Plant Res. 43 (Abstr.).