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http://dx.doi.org/10.7235/hort.2012.12004

Seed Production Ability of Doubled Haploid Plants through Microspore Culture in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) Introduced from China  

Jo, Man Hyun (Future High-Technology Agriculture Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Ham, In Ki (Future High-Technology Agriculture Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Park, Min Young (Department of Horticulture, Chungnam National University)
Kim, Tae Il (Future High-Technology Agriculture Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Lim, Yong Pyo (Department of Horticulture, Chungnam National University)
Lee, Eun Mo (Future High-Technology Agriculture Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Publication Information
Horticultural Science & Technology / v.30, no.5, 2012 , pp. 573-578 More about this Journal
Abstract
A total of eleven Chinese cabbage accessions were used for microspore culture and were grown to take basal data. Based on the collected data, breeding materials were chosen to develop new improved Chinese cabbage cultivars. The range of microspore-derived embryoid taken from flower buds was 1.6 to 35.4 embryoids. The embryoids from IT26110 and IT26153 among the Chinese cabbages were more than 34 per flower bud. The viability rate after cold treatment was low from 0.2 to 11.7%. The range of fertility rate was 7.7 to 58.8% in general but the IT26118, IT26122, IT26128, IT26130, and IT26164 were more than 50%. The result of their seed production ability by selfing was 11.9 seeds per siliqua in IT26128 while the others were less than 10 seeds. In the microspore culture using parents of different hereditary, the number of embryoids, the number of plants, the rate of fertility and their pure seed production ability appeared to be very different in doubled haploid lines obtained from fertile plants of Chinese cabbage.
Keywords
cold treatment; fertility; microspore-derived embryoid; NLN medium; siliqua;
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