• 한국미생물·생명공학회지
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• 제22권5호
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• pp.495-498
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• 1994

Genistein, a inhibitor of the progression of G$_{1}$ and G$_{2}$ phase of the mammalian cell cycle, was discovered through a unique screening system, in which effects of microbial metabolites on the cycle progression of the cultured mouse mammalian carcinoma cell were monitored by flow cytometry. The inhibitor was extracted from the fermentation broth of Streptomyces sp. ZF10 with ethyl acetate, and purified by silica gel column chromatography and HPLC.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3191-3196
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• 2007

The present study addresses a method to operate a fuel-cell system effectively using a recirculation ejector which recycles wasted hydrogen gas. Configuration of a recirculation ejector is changed to investigate the flow behavior through it under varying operating conditions, and how such conditions affect the fuel-cell hydrogen cycle. The numerical simulations are based on a fully implicit finite volume scheme of the axisymmetric, compressible, Reynolds-Averaged, Navier-Stokes equations for hydrogen gas, and are compared with available experimental data for validation. The results show that a hydrogen recirculation ratio is effectively controlled by a configurational alteration within the operational region in which the recirculation passage doesn't plugged by a sonic line.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6417-6421
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• 2015

Viscum album var (VAV) also known as mistletoe, has long been categorized as a traditional herbal medicine in Asia. In addition to its immunomodulating activities, mistletoe has also been used in the treatment of chronic hepatic disorders in China and Korea. There are numerous reports showing that VAV possesses anti-cancer effects, however influence on human hepatocarcinoma has never been elucidated. In the present study, hot water extracts of VAV was evaluated for its potential anti-cancer effect in vitro. SK-Hep1 cells were treated with VAV (50-400ug/ml) for both 24 and 48 hours then cell viability was measured by cell counting kit-8 (CCK-8). Flow cytometry analysis was used to measure the proportion of SK-Hep1 in the different stages of cell cycle. RT-PCR and Western blot analysis were conducted to measure expression of cell cycle arrest related genes and proteins respectively. VAV dose dependently inhibited the proliferation of SK-Hep1 cells without any cytotoxicity with normal Chang liver cell (CCL-13). Flow cytometry analysis showed that VAV extract inhibited the cell cycle of SK-Hep1 cells via G1 phase arrest. RT-PCR and Western blot analysis both revealed that cyclin dependent kinase 2 (Cdk2) and cyclin D1 gene expression were significantly down regulated while p21 was upregulated dose dependently by VAV treatment. Combined down regulation of Cdk2, Cyclin D1 and up regulation of p21 can result in cell death. These results indicate that VAV showed evidence of anti-cancer activity through G1 phase cell cycle arrest in SK-Hep1 cells.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제13권2호
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• pp.123-140
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• 2009

The effect of a solute concentration difference on the osmotic transport of water through the semi-permeable membrane of a simple cell model is investigated. So far, most studies on osmotic phenomena are described by simple diffusion-type equations ignoring all fluid motion or described by Stokes flow. In our work, as the governing equations, we consider the coupled full Navier-Stokes equations which describe the fluid motion and the full transport equation that takes into account of convection and diffusion effects. A two dimensional finite difference model has been developed to simulate the velocity field, concentration field, and semi-permeable membrane movement. It is shown that the cell swells to regions of lower solute concentration due to the uneven water flux through the semi-permeable membrane. The simulation is applied on a red blood cell geometry and the relevant results are presented.

• 한국ITS학회 논문지
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• 제11권6호
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• pp.31-39
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• 2012

본 연구에서는 기존 기종점 트립 기반 교통시뮬레이션이 가지는 한계를 극복하고 대규모 지역이나 도시를 대상으로 개별 통행주체의 활동기반(activity-based) 모형의 적용가능성을 검증하기 위한 기초 연구로 단속류 가로구간을 대상으로 차량추종모형의 근간이 되는 가 감속 및 차로변경 등의 제어변수를 우리 실정에 맞게 보정(calibration)하는 방안을 제시하였다. 특히, 기존 한 cell의 크기인 7.5m보다는 1.0m가 단속류의 교통특성을 보다 잘 구현함을 확인하였으며 각 차량들에 ID를 부여하여 차량의 거동 특성을 추적하는 기법을 제시하였다. 나아가 차종을 세분화하고 대상구간의 차종비율을 적용시켜 실제 단속류에서의 차량의 주행특성을 분석하였다. 제안한 모델의 검증을 위해 대구시 수성구의 달구벌대로 일부 가로구간을 대상으로 시뮬레이션 한 결과 실제 단속류의 교통특성 구현이 가능함을 확인하였다.

• Fisheries and Aquatic Sciences
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• 제24권1호
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• pp.1-9
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• 2021

Human prostate cancer is the second most frequently diagnosed cancer worldwide, and its incidence rate continues to increase. Advanced prostate cancer is more difficult to treat than early forms due to its chemotherapy resistance. There is need for more effective agents that can inhibit the progression of advanced prostate cancer. Demethoxyfumitremorgin C (DMFTC) was isolated from the fermentation extract of the marine fungus Aspergillus fumigatus. Antiproliferative activity of DMFTC against human prostate cancer PC3 cells was examined through cell cycle analysis by flow cytometry, the fluorescent nuclear imaging analysis with propidium iodide (PI), and proteins expression related to cell cycle arrest and apoptosis were investigated via Western blotting. DMFTC inhibited PC3 cells growth through G1 phase cell cycle arrest and apoptosis induction. It activated the tumor suppressor p53 and the Cdk inhibitor p21, which regulate the cell progression into the G1 phase. Additionally, PI-positive late apoptotic non-viable cells were increased and the expression levels of the G1-positive downstream regulators cyclin D, cyclin E, Cdk2, and Cdk4 were decreased by DMFTC treatment. These results suggest that DMFTC induces G1 arrest and apoptosis induction through regulation of p53/p21-dependent cyclin-Cdk complexes, and it may be a useful therapeutic agent for the treatment of human advanced prostate cancer.

• 한국수소및신에너지학회논문집
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• 제23권4호
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• pp.293-299
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• 2012

The serpentine flow channel is widely used in polymer electrolyte membrane fuel cells (PEMFCs) to prevent flooding phenomena because it effectively removes liquid water in the flow channel. The pressure drop between inlet and outlet increases as compared with straight channels due to minor losses associated with the corners of the turning configurations. This results in a strong pressure gradient between adjacent channels in specific regions, where some amount of reactant gas can be delivered to catalyst layers by convection through a gas diffusion layer (GDL). The enhancement of the convective flow in the GDL, so-called bypass flow, affects fuel cell performance since the bypass flow influences the reactant transport and thus its concentration over the active area. In the present paper, for the bipolar plate design, a simple analytic model has been proposed to predict the bypass flow in the serpentine type flow channels and validated with three-dimensional numerical simulation results.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.240-244
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• 2004

Characteristics of steady and unsteady cavitation in a turbopump inducer were investigated in this paper. To see the effect of tip clearance on the inducer performance, three cases of tip clearance were tested. The helical inducer, which has two blades with inlet tip blade angle of 7.8 degree and tip solidity of 2.7, was tested in the water. In the non-cavitating condition, the inducer head decreased with increase in the tip clearance. Rotating cavitation and cavitation surge were observed through unsteady pressure measurements at the inducer inlet. The cell number and propagation speed of the rotating cavitation were determined through cross-correlation analysis. During the rotating cavitation one cell rotated at the same rotational speed as that of the inducer rotation and the cavitation surge did not rotate. The critical cavitation number increased with increase in the tip clearance at the same flow rate, but the change of critical cavitation number was small at the nominal flow rate.

• 한국디지털건축인테리어학회논문집
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• 제12권3호
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• pp.5-13
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• 2012

In this study, features and design effects of PV(Photovoltaic) modules were classified to help the installation of BIPV(Building Integrated Photovoltaic) In addition, through domestic and international trends and cases survey, installation method was organized and applicable range of efficiency and design from First-generation solar cells to the third-generation solar cell was classified. Frist, Crystalline Solar cell module of first-generation is appropriate for the wall type, roof, louver, shading and etc. It has superiority of technology and price stability and can be achieved by a variety of aesthetic effects. Second, Dye-Sensitized Solar Cell of Thin Film solar cell can express a variety of colors, adjust light transmittance and maximize the aesthetic splendor. It is appropriate for the wall type, window type, curtain wall type and etc. Also, see-through type solar cell can provide comforts cause of free flow of light. And it is advantageous from economic due to adjust the indoor temperature. It is appropriate for the atrium type, curtain wall type, window type and etc.

• 한국가스학회지
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• 제27권1호
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• pp.38-47
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• 2023

수소연료전지 차량 충전 과정에서, 충전소에서의 공급압력과 차량 내 저장 탱크의 압력 차이에 의해 수소가 흐르게 되고 유량은 압력 차에 의존한다. 따라서 충전 과정에서 발생하는 수소의 압력강하에 대한 고려는 필수적이며 이의 분석을 통해 수소 충전 과정의 효율성을 높일 수 있다. 본 연구에서는 충전라인 중 호스, 노즐/리셉터클, 파이프, 밸브에 대하여 압력강하를 분석하였다. 호스와 파이프는 도관에서의 압력강하로, 노즐/리셉터클은 흐름 노즐 식으로, 밸브는 기체 유량 식으로 계산하였다. 또한 각 구성요소에서 발생하는 압력강하 효과를 종합 분석한 결과 전체 충전라인에서 압력강하에 가장 큰 영향을 주는 요소는 밸브에서의 압력강하임이 밝혀졌다. 이번 연구는 추후 수소 충전을 포함한 수소 유동 해석으로 수소 충전 과정의 모델 개발에 활용될 수 있을 것이다.