• Journal of Advanced Marine Engineering and Technology
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• v.40 no.3
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• pp.180-184
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• 2016

Legislative and regulatory actions regarding the exhaust gas from ships are being strengthened by both international organizations and national governments, to protect human health and the environment. Exhaust gas traps are excluded from exhaust gas regulation applications, but, recently, the United States, Britain, and other developed countries have examined a variety of ways to improve the system, including the introduction of electric propulsion systems to prevent air pollution generated by naval ships. This study investigates a large number of smoke problems of naval diesel engines to verify the effect of improving the nozzle characteristics. An exhaust gas emission measurement method to determine the quality of pollutant exhaust gas generated during low-load operation is proposed through the research methodology of the smoke problem. It was confirmed that the emissions value is improved by decreasing the nozzle hole diameter and increasing the injection pressure. At the same time, the flow rate decrease equation and setting up a test memo based on the nozzle diameter confirmed that the fuel consumption, to which the nozzle diameter in the flow path is related, is reduced.

• Applied Chemistry for Engineering
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• v.21 no.6
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• pp.632-637
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• 2010

Considering the water quality regulation of total emission system which is enforced with hydro TMS and so on operating currently, automatic water sampler must be necessary in the viewpoint of national interest for conservation of water resources. This study aimed to develope automatic water sampler for the purpose of decreasing spends on foreign currency which is relied on import from abroad and upgrading the functional efficiency simultaneously. We have made an effort for developing results as follows with some cases. First, flow meter with a sensor equiped into automatic water sampler for field measurement which can perform linkage operation was developed. Second, remote-D/L system was developed which was able to monitor and store some transmitted data from practical measurement sensor. Also, automatic water sampler was developed in this research that operates in a sewage treatment plant, and finally, we confirmed that our system is able to apply to the field well.

• Journal of Life Science
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• v.12 no.4
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• pp.469-476
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• 2002

The DNA topoismerase I inhibitor $\beta$-lapachone, the product of a tree from South America, is known to exhibit various biological properties, however the mechanisms of which are poorly understood. In the present report, we investigated the effects of $\beta$-lapachone on the growth of human prostate carcinoma DU-145 cells. Upon treatment with $\beta$-lapachone, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and DNA fragmentation. Flow cytometry analysis confirmed that $\beta$-lapachone increased populations of apoptotic-sub Gl phase. In addition, proteolytic cleavages of poly (ADP-ribose) polymerase (PARP) and $\beta$-catenin protein were observed after treatment of $\beta$-lapachone. These apoptotic effects of $\beta$-lapachone in DU-145 cells were associated with marked induction of Bax protein, however the levels of Bcl-2 expression were decreased in a dose-dependent manner.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.15 no.4
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• pp.259-268
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• 2003

The damage of water quality like red tide occurs every year inspite of the total load regulation of pollutant inflows from land such as COD, phosphorus and so on around Seto inland sea in Japan. It is attributed to the increase of primary production due to eutrophication. and the rising and settling from the sediment containing nutrient salt. Furthermore, the recent research shows that nutrient salt such as nitrogen and phosphorus, flows into Kii channel from land as well as the Pacific ocean. To investigate the distribution of water quality and flow and residual current in Kitan strait, the field observations were carried out. The field data were obtained a time each season from 1999 to 2002. The results of observations and data of other research institution are used to understand the mechanism of pollution in the strait.

• The Journal of Internal Korean Medicine
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• v.35 no.3
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• pp.317-331
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• 2014

Objectives: The root of Platycodon grandiflorum (PG) has been known to possess a range of pharmacological activities including anti-cancer, anti-inflammatory, and anti-oxidant effects. The present study was designed to investigate whether or not PG-induced cell death was connected with autophagy and apoptosis in NCI-H460 human lung cancer cells. Methods: Effects on the cell viability and apoptotic activity were quantified using MTT assays and flow cytometry analysis, respectively. Protein activation was measured by immunoblotting. Autophagy was measured by LC3 immunofluorescence and immunoblotting. ROS production and loss of mitochondria membrane potential (MMP) were checked with flow cytometry analysis. Results: Following exposure to PG, NCI-H460 cell proliferation decreased simultaneously inducing autophagic vacuoles and up-regulation of microtubule-associated protein 1 light chain 3 and beclin-1 protein expressions. Interestingly, pre-treated with autophagy inhibitors, 3-methyladenin or bafilomycin A1 further triggered reduction of cell viability. PG treatment also induced apoptosis that was related modulation of Bcl-2 family proteins, death receptors and activation of caspases. In addition, PG stimulation clearly enhanced loss of MMP and reactive oxygen species (ROS) generation. Conclusions: Our results suggest that PG elicited both autophagy and apoptosis by increasing loss of MMP and ROS production. PG induced-autophagy may play a cell protective role.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.220-223
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• 2011

Human basophilic KU812F cells express a high affinity for IgE receptor, $Fc{\varepsilon}RI$, which plays an important role in immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on $Fc{\varepsilon}RI$ ${\alpha}$ chain expression. Flow cytometric analysis showed that cell surface $Fc{\varepsilon}RI$ ${\alpha}$ chain expression was suppressed when the cells were cultured with CZ extract. RT-PCR analysis showed that the mRNAs for $Fc{\varepsilon}RI$ ${\alpha}$ chain decreased by the treatment of CZ extract. Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation of $Fc{\varepsilon}RI$ ${\alpha}$ chain expression and a subsequent decrease in histamine release.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.38 no.2
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• pp.155-163
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• 2014

At gas stations, liquid fuels expand and contract in volume owing to temperature variations. In Korea, the ambient temperature varies between $-15^{\circ}C$ in winter and $35^{\circ}C$ in summer. The volume expansion coefficients of liquid fuels are about $0.1%/^{\circ}C$. To investigate this issue, we measured daily changes in fuel temperature and the delivered fuel temperature at gas stations. In addition, we scrutinized the daily, monthly, and annual changes in temperature over past 50 years in Korea. The results show that the temperature of the fuel in the storage tank was maintained at a stable value(summer or winter). Many factors, such as the surrounding conditions, fuel filling frequency, and gas station location, influence the delivered fuel temperature. The results of this study can be applied for establishing a national regulation and will contribute to fair transactions.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.1
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• pp.273-276
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• 2014

Ellagic acid has been shown to inhibit tumor cell growth. However, the underlying molecular mechanisms remain elusive. In this study, our aim was to investigate whether ellagic acid inhibits the proliferation of MCF-7 human breast cancer cells via regulation of the TGF-${\beta}$/Smad3 signaling pathway. MCF-7 breast cancer cells were transfected with pEGFP-C3 or pEGFP-C3/Smad3 plasmids, and treated with ellagic acid alone or in combination with SIS3, a specific inhibitor of Smad3 phosphorylation. Cell proliferation was assessed by MTT assay and the cell cycle was detected by flow cytometry. Moreover, gene expression was detected by RT-PCR, real-time PCR and Western blot analysis. The MTT assay showed that SIS3 attenuated the inhibitory activity of ellagic acid on the proliferation of MCF-7 cells. Flow cytometry revealed that ellagic acid induced G0/G1 cell cycle arrest which was mitigated by SIS3. Moreover, SIS3 reversed the effects of ellagic acid on the expression of downstream targets of the TGF-${\beta}$/Smad3 pathway. In conclusion, ellagic acid leads to decreased phosphorylation of RB proteins mainly through modulation of the TGF-${\beta}$/Smad3 pathway, and thereby inhibits the proliferation of MCF-7 breast cancer cells.

• The Korean Journal of Physiology
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• v.22 no.2
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• pp.319-332
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• 1988

The regulations of renal function and renin release are influenced by neural, humoral and physical factors. During the last decade, considerable progress has been made in the identification and characterization of these extrinsic renal control systems. Mechanisms intrinsic to the kidney are also important for renal function. These include the autoregulation of blood flow, and the local control of renin secretion. Fundamental questions regarding the mechanism of these intrinsic controls remain unanswered. Recently, endogenous renal adenosine has been claimed to influence the tubuloglomerular feedback control and renin release. Two subclasses of adenosine receptors $A_1{\;}and{\;}A_2$ have been described. The present experiment was carried out to evaluate the effects of $N_6-cyclohexyladenosine$ $(CHA,{\;}A_1{\;}selective)$ and 5'-N-ethylcarbox-amide adenosine $(NECA,{\;}A_2{\;}selective)$ on the renal function and renin release in the unanesthetized rabbit. Intra-renal arterial infusion of NECA $(0.3{\sim}10.0n{\;}mole/min/rabbit)$ or CHA $(0.03{\sim}10.0n{\;}mole/min/rabbit)$ caused a prompt and dose-dependent decrease in urine volume, glomerular filtration rate (GFR), renal plasma flow (RPF), filtration fraction (FF), electrolyte excretion and free water clearance $(CH_2O)$, the effect being much more profound with CHA than with NECA. The NECA infusion resulted in a profound decrease of systemic blood pressure, but the CHA infusion did not. Both NECA and GHA infusions caused a prompt and dose-dependent decrease in renin secretion rate, again the effect being greater with CHA than with NEGA. These results suggest that both $A_1{\;}and{\;}A_2$ adenosine receptors may be involved in the intrinsic control of renal function and renin release, and that the $A_1$ receptors plays a more important role than the $A_2$ receptor in the regulation of renal fnction.

• Journal of Korea Water Resources Association
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• v.39 no.7 s.168
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• pp.627-636
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• 2006

This study estimates adequate discharge to meet the specified target water quality concentration using the pollutant load of the Geum river basin given in TMDL (Total Maximum Daily Load) report. During the 1st phase, BOD is chosen as a target water quality constituent under regulation of the Ministry of Environment in Korea. BOD, TN, and TP loads estimated based on the TMDL and provincial zones were re-distributed for 10 major tributaries, and the remaining areas along the main river are classified as 15 incremental flow areas. Water quality modeling was conducted using Qual2E for the low flow period of a year (i.e. $March{\sim}April$). The results of the model simulation showed that about 30 cms from the Daechung dam would be sufficient to satisfy the target water quality in the Geum river downstream of the Daechung multipurpose Dam.