• Journal of Interdisciplinary Genomics
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• v.3 no.2
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• pp.41-46
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• 2021

Inherited platelet function disorders (IPFDs) are a disease group of heterogeneous bleeding disorders associated with congenital defects of platelet functions. Normal platelets essential role for primary hemostasis by adhesion, activation, secretion of granules, aggregation, and procoagulant activity of platelets. The accurate diagnosis of IPFDs is challenging due to unavailability of important testing methods, including light transmission aggregometry and flow cytometry, in several medical centers in Korea. Among several IPFDs, Glanzmann thrombasthenia (GT) is a most representative IPFD and is relatively frequently found compare to the other types of rarer IPFDs. GT is an autosomal recessive disorder caused by mutations of ITGA2B or ITGB3. There are quantitative or qualitative defects of the GPIIb/IIIa complex in platelet, which is the binding receptor for fibrinogen, von Willbrand factor, and fibronectin in GT patients. Therefore, patients with GT have normal platelet count and normal platelet morphology, but they have severely decreased platelet aggregation. Thus, GT patients have a very severe hemorrhagic phenotypes that begins at a very early age and persists throughout life. In this article, the general contents about platelet functions and respective IPFDs, the overall contents of GT, and the current status of genetic diagnosis of GT in Korea will be reviewed.

• Journal of Korea Multimedia Society
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• v.23 no.12
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• pp.1540-1551
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• 2020

Aiming at the problem that the existing human behavior recognition algorithm cannot fully utilize the multi-level spatio-temporal information of the network, a human behavior recognition algorithm based on a dense three-dimensional residual network is proposed. First, the proposed algorithm uses a dense block of three-dimensional residuals as the basic module of the network. The module extracts the hierarchical features of human behavior through densely connected convolutional layers; Secondly, the local feature aggregation adaptive method is used to learn the local dense features of human behavior; Then, the residual connection module is applied to promote the flow of feature information and reduced the difficulty of training; Finally, the multi-layer local feature extraction of the network is realized by cascading multiple three-dimensional residual dense blocks, and use the global feature aggregation adaptive method to learn the features of all network layers to realize human behavior recognition. A large number of experimental results on benchmark datasets KTH show that the recognition rate (top-l accuracy) of the proposed algorithm reaches 93.52%. Compared with the three-dimensional convolutional neural network (C3D) algorithm, it has improved by 3.93 percentage points. The proposed algorithm framework has good robustness and transfer learning ability, and can effectively handle a variety of video behavior recognition tasks.

• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.1
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• pp.117-133
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• 2008

Purpose: This study was performed to evaluate anti-thrombotic effects of Hyulbuchukeotanggamibang(HBCT). Methods: It was measured the effects which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Results: 1. HBCT showed a safety in toxicity of liver. 2. In experiment of anti-thrombotic effect, HBCT inhibited the platelet aggregation induced by ADP and epinephrine, collagen, arachidonic acid as compared with the control group. 3. HBCT inhibited pulmonary embolism induced by collagen and epinephrine (inhibitory rate is 50%). 4. HBCT increased platelet number and fibrinogen amount significantly and also HBCT shortened PT and APTT significantly as compared with the control group in thrombus model induced by dextran. 5. HBCT increased blood flow rate insignificantly as compared with the control group in vivo. Conclusion: These results suggest that HBCT can be used for treating diverse female diseases caused by thrombosis.

• The Journal of Internal Korean Medicine
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• v.18 no.1
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• pp.69-85
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• 1997

This study was designed to elucidate the anti-inflammatory, cardiovascular, anti-thrombotic and analgesic effects of Shintongchugeotang. The anti-inflammatory effect was measured by the method of carragenin induced edema, protein leakage test using CMC-pouch, and the analgesic effect was measured by the acetic acid method and hot plate method, and the effect of Shintongchugeotang on the cardiovascular system was observed by the change of flow rate of Ringer solution in the vascular system in the ear of rabbit, and the contraction and dilatation of rat tail artery. Death rate, platelet aggregation, plasma coagulation activity was observed for the measurement of the anti-coagurative effect of Shintongchugeotang. The result was as follows : 1. After the administration of Shintongchugeotang extract, Carragenin induced edema and CMC-pouch protein leakage were significantly decreased. 2. The slight analgesic effect of Shintongchugeotang extract was confirmed by the observation of writhing syndrome, paw licking time, and escape time. 3. The drug increased the auricular blood flow in rabbit. 4. The drug relaxed the artery contraction by pretreated norepinephrine in rat. 5. The drug inhibited the death rate of mouse which was led to thromboembolism by serotonin and collagen. 6. The drug inhibited the platelet aggregation in rat. 7. The drug prolonged the prothrombin time and activated partial thromboplastin time on the test of plasma coagulation factor activity in rat, but was not valuable.

• The Journal of Internal Korean Medicine
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• v.19 no.2
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• pp.88-106
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• 1998

This study was designed to elucidate the antiinflammatory, cardiovascular, antithrombotic, and analgesic effect of Sambitang. The antiinflammatory effects was measured by the method of carrageenin induced edema, protein leakage test using CMC-pouch, and the effect of Sambitang on the cardiovascular system was observed by the change of flow rate of Ringer solution in the vascular system in the ear of rabbit, and the contraction and dilatation of rat tail artery. Death rate, platelet aggregation, plasma coagulation activity was observed for the measurement of the anticoagurative effect of Sambitang, and the analgesic effect was measured by the acetic acid method and hot plate method. The result was as follows: 1. Sambitang administration, edema and protein leakage was significantly decreased. 2. The drug increased the auricular blood flow in rabbit. 3. The drug relaxed the artery contraction by pretreated norepinephrine in rat. 4. The drug inhibited the death rate of mouse which was led to thromboembo- lism by serotonin and collagen. 5. The drug inhibited the platelet aggregation in rat. 6. The drug prolonged the prothrombin time and activated partial thromboplastin time on the test of plasma coagulation factor activity in rat, but was not valuable. 7. The slight anagesic effect of Sambitang extract was confirmed by the observation of writhing syndrome, paw licking time, and escape time.

• KSBB Journal
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• v.27 no.6
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• pp.375-380
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• 2012

We previously reported that Ultra nattokinase$^{(R)}$ showed high fibrinolytic activity and revealed antithrombotic effect in rat blood plasma based on its ability to suppress collagen-induced platelet aggregation. This research was carried out to verify the clot lysing activity and blood flow enhancing effects of Ultra nattokinase$^{(R)}$ via monitoring and comparing the antithrombotic effects in rat artery between oral administration of Ultra nattokinase$^{(R)}$ and maltodextrin. SD rats were fed with 1.11 mg/kg of Ultra nattokinase$^{(R)}$ for 4 weeks. The effect on arterial thrombosis was then evaluated using an antithrombotic model after induction by $FeCl_3$. Detected fibrinolytic activity was proportional to the content of Ultra nattokinase$^{(R)}$ and statistical extents of the antithrombotic activity was enhanced strongly twice rather than control group. The PT and the aPTT, however, showed only a small difference between two groups. The results suggest that Ultra nattokinase$^{(R)}$ can effectively treat thromboembolism and enhance blood flow, and that Ultra nattokinase$^{(R)}$ can also prevent venous occlusion by aiding clot lysis.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.1-17
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• 2006

Purpose : The Purpose of this research was to investigate the effects of antithrombotic activities of Haenggyonghonghwatang (HGHHT). Methods : Measured the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Each of the groups consisted in 8 mice, was divided into Normal, Control, and HGHHT. All of these 3 group were supplied a saline solution and after an hour the control group brought the dextran extravasated blood. Also the HGHHT group was dosed to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood as well as the Control group. Results : The results were obtained as follows. HGHHT inhibited platelet aggregation induced by ADP and epinephrine significantly as compared with the control group. HGHHT showed fibrinolytic activity insignificantly as compared with the control group. HGHHT increased blood flow rate significantly as compared with the control group in vitro, but insignificantly as compared with the control group in vivo. HGHHT inhibited pulmonary embolism induced by collagen and epinephrine(inhibitive rate 50%). HGHHT increased number of platelet, fibrinogen amount and shortened prothrombin time, activated partial thromboplastin time significantly as compared with the control group in thrombus model induced by dextran. Conclusion : HGHHT is effective antithrombotic activity from experimental result.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.1
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• pp.181-185
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• 2002

The thrombosis is the category of blood stasis. Blood stasis is a pathologicial state resulting from the reverse or impeded flow of blood in the body or the stagnation of blood flow in local parts as well as abnormal blood outside of the vessles which remains in the body and fails to disperse. Hwao-tang has been reported to have a hypolipidemic effect in patients with hypercholesterolemia, and in highcholesterol-induced experimental models. The present paper reports the effects of HOT on atherosclerosis using a spontaneous experimental model, Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbits. We have also investigated the pharmacological effect of extracts obtained from HOT on collagen-and ADP-induced blood platelet aggregation, thrombin-induced conversion of fibrinogen and fibrinolysis in in vitro experiments. In conclusion, the protection of extracts of Korean herbs' HOT on the ischemic infarction induced artificially might be involved to their inhibition of thrombotic action.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.05b
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• pp.73-76
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• 2005

This study aims to suggest a simple and convenient design for a mix proportion method for high performance concrete by determining the optimum fine aggregate ratio and minimum binder content based on the maximum density theory. The mix design method introduced in this study adopted the optimum fine aggregate ratio with a minimum void and binder content higher than the minimum binder content level. The research results reveal that the method helps to reduce trial and error in the mixing process and is a convenient way of producing high performance concrete with self filler ability. In an experiment based on the mix proportion method, when aggregate with the fine aggregation ratio of 41$\%$ was used, the minimum binder content of high performance concrete was 470kg/$m^{3}$ and maximum aggregate capacity was $0.657m^{3}/m^{3}$. In addition, in mixing high performance concrete, the optimal slump flow to meet filler ability was 65$\pm$5cm, V load flow speed ranged from 0.5 to 1.5.

• Journal of Life Science
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• v.17 no.4 s.84
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• pp.575-580
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• 2007

The purpose of this study was to investigate the effect of three different mulberry cultivars extracts on inhibition of platelet aggregation and blood flow improvement. Four groups were surgically ovariectomized (OVX). The fifth group was sham operated. Sprague-Dawley strain female rats were randomly assigned to the following groups. sham-operated rats(Sham), ovariectomized control rats(OVX-Control), ovariectomized rats supplemented with 80% ethanol extracts from fruits of Tajikistan mulberry (OVX-TM), Korea mulberry (OVX-KM) and China mulbery (OVX-CM) at 200 mg/kg bw/d. The mulberry extracts were orally administrated at 1 ml per day. The body weights of OVX rats were significantly heavier than the sham-operated rats at all values (p<0.05). The serum triglyceride level was significantly decreased after supplemented with the CM and KM extracts. The serum HDL-cholesterol level in the OVX-KM group was significantly higher than that in the OVX-control group. The passage time of whole blood showed significant correlation with triglyceride but reverse correlation with HDL cholesterol. The ability of platelet aggregation of groups treated with mulberry extracts was less than OVX-control group. Taken these together, mulberry extracts may be used to possibly improve the quality of life in menopausal women.