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http://dx.doi.org/10.7841/ksbbj.2012.27.6.375

The Evaluation of Antithrombotic and Fibrinolytic Activities of Nattokinase from Bacillus subtilis Natto  

Lee, Da-Lyung (Cardiovascular product evaluation center Yeonsei university collage of medicine)
Hong, Sung-Yu (Cardiovascular product evaluation center Yeonsei university collage of medicine)
Jang, Yang-Su (Cardiovascular product evaluation center Yeonsei university collage of medicine)
Jang, Hyung-Wook (KPX Bio-Tech Co., LTD.)
Maeng, Chang-Jae (KPX Bio-Tech Co., LTD.)
Yoo, Chul-Bae (KPX Bio-Tech Co., LTD.)
Baek, Dae-Heoun (KPX Bio-Tech Co., LTD.)
Publication Information
KSBB Journal / v.27, no.6, 2012 , pp. 375-380 More about this Journal
Abstract
We previously reported that Ultra nattokinase$^{(R)}$ showed high fibrinolytic activity and revealed antithrombotic effect in rat blood plasma based on its ability to suppress collagen-induced platelet aggregation. This research was carried out to verify the clot lysing activity and blood flow enhancing effects of Ultra nattokinase$^{(R)}$ via monitoring and comparing the antithrombotic effects in rat artery between oral administration of Ultra nattokinase$^{(R)}$ and maltodextrin. SD rats were fed with 1.11 mg/kg of Ultra nattokinase$^{(R)}$ for 4 weeks. The effect on arterial thrombosis was then evaluated using an antithrombotic model after induction by $FeCl_3$. Detected fibrinolytic activity was proportional to the content of Ultra nattokinase$^{(R)}$ and statistical extents of the antithrombotic activity was enhanced strongly twice rather than control group. The PT and the aPTT, however, showed only a small difference between two groups. The results suggest that Ultra nattokinase$^{(R)}$ can effectively treat thromboembolism and enhance blood flow, and that Ultra nattokinase$^{(R)}$ can also prevent venous occlusion by aiding clot lysis.
Keywords
Bacillus subtilis Natto; Ultra nattokinase$^{(R)}$; Antithrombotic effect; Fibrinolytic effect; Platelet aggregation;
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