• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1163-1172
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• 2016

The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the T_m values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.

• 한국전산구조공학회논문집
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• 제27권5호
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• pp.379-384
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• 2014

본 논문은 갠트리 크레인의 설계를 위해 컨테이너 하역작업 시 특정하중 조건하의 RTGC(Rubber Tired Gantry Crane)의 동적거동과 그에 따른 윤하중을 분석한 내용을 기술하고 있다. 먼저 RTGC의 동적거동을 살펴보기 위해 거대 구조물인 크레인의 유한요소 모델을 개발하고 고유진동수와 고유모드의 모달시험결과를 이용하여 유한요소모델을 검증하였다. RTGC의 기타 부속품은 3차원 CAD모델링을 통해 다물체 동역학해석 소프트웨어인 ADAMS에서 강체로 모델링하였다. 본 연구에서 하중 조건은 일반적인 컨테이너의 이송조건(OP1)과 외부부하조건 없이 단순히 트롤리를 이용하여 컨테이너를 하역하는 2가지 경우로 고려하였다. 해석 결과 RTGC의 컨테이너 작업 시 발생하는 크레인의 진동은 거대 구조물의 강성과 변형에 주로 기인함을 확인하였으며 이러한 크레인의 진동은 RTGC의 움직임을 발생시켜 컨테이너 하역작업 불능 등의 거동을 발생시킬 수 있음을 분석할 수 있었다.

• 한국정밀공학회지
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• 제28권8호
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• pp.975-983
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• 2011

A microfin on a heated surface and its effects of the heat transfer has been investigated. The thickness of the fin is about 8 micrometer to allow the flexible up-down motion of the fin. Two-way complete FSI (Fluid-Structure Interaction) method has been applied for the analysis. Firstly, the deformation of a microfin due to the pulsating flow is evaluated using structure analysis. The flow and temperature patterns are predicted by CFD (Computational Fluid Dynamics) method. At each time step, using the pressure force and temperature distribution from CFD, the deformation of the wing is evaluated by FEM. Also in order to estimate the resonance probability, the natural frequency of the wing structure is calculated by modal analysis. The proposed numerical procedure was validated through experiment using a single fin. Through this work, we show that the increase of 40% in heat transfer capacity using the microfin has been compared with that of flat plate case.

• 대한기계학회논문집A
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• 제34권4호
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• pp.501-509
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• 2010

해상크레인은 크레인을 탑재한 선박으로서, 조선소에서 대형 블록이나 구조물의 탑재 및 해상 운송 작업에 사용된다. 본 논문에서는 해상크레인과 중량물의 전후 동요(Surge), 상하 동요(Heave), 종 동요(Pitch)에 대한 정적/동적거동을 분석하였다. 이 때, 유연 다물체계 동역학을 적용하여 해상크레인의 붐(boom)을 탄성으로 고려하였으며, 플로팅 프레임(floating frame)과 노드 좌표(nodal coordinates)를 사용하였다. 질량 행렬, 탄성 강성 행렬, 2 차 속도 벡터, 일반화 좌표 방향으로 작용하는 외력 등을 고려하여 모든 운동이 연성된 비선형 운동 방정식을 구성하였다. 외력으로는 비선형 유체정역학 힘, 선형화된 유체동역학 힘, wire rope 힘, 계류력이 고려되었다. 수치 해석을 위해 Hilber-Hughes-Taylor 방법을 비선형 운동방정식에 적용하였다. 정적 거동 분석을 통한 정적 평형 자세를 고려한 경우와 고려하지 않은 경우에 대해 결과를 비교하였으며, 수치 해석 방법에 대한 정적/동적 거동 분석 결과를 비교하였다.

• Ocean and Polar Research
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• 제37권2호
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• pp.119-125
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• 2015

This paper focuses on the efficient arrangement plan of buoyancy modules, which plan is used to secure the safe operation and structural stability of a marine riser. The marine riser is connected between a vessel and seabed devices. The movement of the vessel and the seabed devices are affected by the motion of the riser. The riser of a deep-seabed integrated mining system exerts a strong influence on the healthy transfer of minerals. So, buoyancy modules must be equipped to compensate for the problem which is the structure stability and the dynamic motion. Installation locations and quantities of the buoyancy modules are determined by real sea experiments. But this is not easy to do because in real sea experimental conditions the cost is expensive as well as being, time-consuming and dangerous. Therefore, the locations and quantities should be determined by numerical simulation. This method is called simulation-based design. The dynamic analysis models of the riser and the buoyancy modules are built into the commercial software of DAFUL.

• 드라이브 ㆍ 컨트롤
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• 제14권2호
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• pp.23-29
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• 2017

In this study, a flexible multi-body dynamic simulation model of a knuckle boom crane is developed to evaluate the stress of spindle and rack gears under dynamic working conditions. It is difficult to predict potential critical damage to a knuckle boom crane if only the static condition is considered during the development process. To solve this issue, a severe working scenario (high speed with heavy load) was simulated as a boundary condition for testing the integrity of the dynamic simulation model. The crane gear model is defined as a flexible body so contact analysis was performed. The functional motion of a knuckle boom crane is generated by applying forces at each end of the rack gear, which was converted from hydraulic pressure measured for the experiment. The bending and contact stress of gears are theoretically calculated to validate the simulation model. In the simulation, the maximum stress of spindle and rack gears are observed when the crane abruptly stops. Peak impact force is produced at the contact interface between pinion and rack gears due to the inertia force of the boom. However, the maximum stress (bending/contact) of spindle and rack are under the yield stress, which is safe from damage. By using the developed simulation model, the experiment process is expected to be minimized.

• 한국철도학회논문집
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• 제18권1호
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• pp.15-24
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• 2015

상전도흡인식 자기부상열차는 전자석의 전류를 조정하여 차량과 선로 사이의 간극(공극)을 일정하게 유지한다. 이러한 능동현가장치를 사용하는 자기부상열차에 있어서는 다양한 외란에도 불구하고 부상제어기를 이용한 부상공극을 허용하는 범위 내로 유지하는 것이 핵심기술 중의 하나이다. 특히 차량과 유연한 가이드웨이 사이의 동적상호작용에 의해 발생되는 진동은 저속 및 정지상태에서 부상안정성을 방해하는 중요한 요인으로 작용한다. 본 논문에서는 가이드웨이의 유연성에도 강인한 부상제어기를 개발하기 위하여 차량과 유연 가이드웨이 사이의 연성 동역학 모델을 개발하고, 전자석 및 부상제어기도 포함시키는 통합모델링을 제안하였다. 개발된 통합동역학 모델을 이용하여 가이드웨이의 유연성을 고려한 부상공극 시뮬레이션을 수행하였다.

• 대한기계학회논문집 C: 기술과 교육
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• 제5권2호
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• pp.89-95
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• 2017

제품을 개발하는데 있어서 경량화와 원가절감이라는 두가지 요소는 가장 중요한 화두이다. 특히 대형 LCD 로봇은 최대 $3{\times}3m$ 이상의 글라스를 7m 높이까지 상하, 전후로의 직선운동과 선회축을 중심으로한 회전운동을 하면서 작업공정간 이송을 가능케 하는 대형 구조물이다. 따라서 지나치게 무거울 경우에는 클린룸내 설치에 문제가 있을 수 있고 반송물의 정확한 이송을 위하여는 고강성이 요구되며 대량 생산을 위한 연속작업을 충분히 감당할 수 있는 내구강도를 확보하여야 한다. 따라서 경량화, 고강성, 고강도 제품에 대한 요구는 갈수록 증가하고 있다. 현재 개발되고 있는 11 세대 대형 LCD 승강프레임은 이러한 요구조건을 충족하기 위하여 최적설계 기법을 적용하여 기존 제품 대비 경량화와 고강성 요구조건을 만족하였으며 용접부에 대한 상세 수명평가로 내구강도에 대한 신뢰성을 확보하였다.

• Advances in aircraft and spacecraft science
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• 제6권2호
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• pp.117-144
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• 2019

High Altitude and Long Endurance (HALE) aircraft are capable of providing intelligence, surveillance and reconnaissance (ISR) capabilities over vast geographic areas when equipped with advanced sensor packages. As their use becomes more widespread, the demand for additional range, endurance and payload capability will increase and designers are exploring non-conventional configurations to meet the increasing demands. One such configuration is the joined-wing concept. A joined-wing aircraft is one that typically connects a front and aft wings in a diamond shaped planform. One such example is the Boeing SensorCraft configuration. While the joined-wing configuration offers potential benefits regarding aerodynamic efficiency, structural weight, and sensing capabilities, structural design requires careful consideration of elastic buckling resulting from the aft wing supporting, in compression, part of the forward wing structural loading. It has been shown already that this is a nonlinear phenomenon, involving geometric nonlinearities and follower forces that tend to flatten the entire configuration, leading to structural overload due to the loss of the aft wing's ability to support the forward wing load. Severe gusts are likely to be the critical design condition, with flight control system interaction in the form of Gust Load Alleviation (GLA) playing a key role in minimizing the structural loads. The University of Victoria Center for Aerospace Research (UVic-CfAR) has built a 3-meter span scaled and flexible wing UAV based on the Boeing SensorCraft design. The goal is to validate the nonlinear structural behavior in flight. The main objective of this research work is to perform Ground Vibration Tests (GVT) to characterize the dynamic properties of the scaled flight vehicle. Results from the experimental tests are used to characterize the modal dynamics of the aircraft, and to validate the numerical models. The GVT results are an important step towards a safe flight test program.

• International Journal of Aeronautical and Space Sciences
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• 제14권1호
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• pp.30-45
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• 2013

The present work focuses on the unsteady aerodynamics and aeroelastic properties of a small-medium sized wind-turbine blade operating under ideal conditions. A tapered/twisted blade representative of commercial blades used in an experiment setup at the National Renewable Energy Laboratory is considered. The aerodynamic loads are computed using Computational Fluid Dynamics (CFD) techniques. For this purpose, FLUENT$^{(R)}$, a commercial finite-volume code that solves the Navier-Stokes and the Reynolds-Averaged Navier-Stokes (RANS) equations, is used. Turbulence effects in the 2D simulations are modeled using the Wilcox k-w model for validation of the CFD approach. For the 3D aerodynamic simulations, in a first approximation, and considering that the intent is to present a methodology and workflow philosophy more than highly accurate turbulent simulations, the unsteady laminar Navier-Stokes equations were used to determine the unsteady loads acting on the blades. Five different blade pitch angles were considered and their aerodynamic performance compared. The structural dynamics of the flexible wind-turbine blade undergoing significant elastic displacements has been described by a nonlinear flap-lag-torsion slender-beam differential model. The aerodynamic quasi-steady forcing terms needed for the aeroelastic governing equations have been predicted through a strip-theory based on a simple 2D model, and the pertinent aerodynamic coefficients and the distribution over the blade span of the induced velocity derived using CFD. The resulting unsteady hub loads are achieved by a first space integration of the aeroelastic equations by applying the Galerkin's approach and by a time integration using a harmonic balance scheme. Comparison among two- and three- dimensional computations for the unsteady aerodynamic load, the flap, lag and torsional deflections, forces and moments are presented in the paper. Results, discussions and pertinent conclusions are outlined.