• Title/Summary/Keyword: Flavonoid contents

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Evaluation of Antioxidant Activity and Cytotoxicity in Mixture Extract of Artemisia asiatica Nakai and Moringa oleifera Lam

  • Lee, Yoon-Ji;Kim, Jang-Oh;Jeon, Chan-Hee;Lee, Ji-Eun;Shin, Ji-Hye;Min, Byung-In
    • Biomedical Science Letters
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    • v.26 no.4
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    • pp.296-301
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    • 2020
  • The purpose of this study was performed to evaluate antioxidant activity of the Artemisia asiatica Nakai and Moringa oleifera Lam mixture extract. Mixture extracts were manufactured by concentration and compared with a single extract (only the Artemisia asiatica Nakai mixture and only the Moringa oleifera Lam mixture). The experiments conducted Total polyphenol measurements, Total flavonoid measurements, DPPH radical scavenging activty, ABTS radical scavenging activty and LDH assay. The LDH assay assessment shows that all extracts are cells compared to controls. The toxicity was weak. Finally, The antioxidant capacity was rated higher than mixture extract of a single extract. Also, the optimized mixture was determined AM5 (Artemisia asiatica Nakai mixture: Moringa oleifera Lam mixture = 3:1). For extracts of AM5, Total phenol and flavonoid contents were 271.769±18.087 mg/g and 45.384±5.026 mg/g. and DPPH and ABTS scavenging activity were 70.8±6.496% and 77.1±9.634%. Therefore, it is expected that the value of the extract will increase as it increases its antioxidant activity if it is manufactured according to the appropriate ratio.

Preparation and Characterization of Polyurethane Nanofibers Containing Dendropanax morbiferus Extracts (황칠나무 추출물을 함유한 폴리우레탄 나노섬유의 제조 및 특성)

  • Dayae Kang;Jungsoon Lee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.48 no.4
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    • pp.673-687
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    • 2024
  • In this study, we first analyzed the total polyphenol and flavonoid contents and the antioxidant activity of Dendropanax morbiferus extracts obtained from different parts using different solvents. We then investigated the possibility of producing Dendropanax morbiferus leaf extract loaded polyurethane (PU) nanofibers via electrospinning. The total polyphenol and flavonoid content and the antioxidant activity of the Dendropanax morbiferus leaf extracts obtained distilled water were found to be higher than those of the extracts obtained under other conditions, and it was therefore used to prepare the extracts/PU nanofibers by varying the concentrations of both the Dendropanax morbiferus leaf extract and PU. The most appropriate fiber morphology were when 1.5% and 1.0% of the Dendropanax morbiferus leaf extracts were added to 12% and 13% PU, respectively. Fourier-tranform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD) analyse reveal the successful incorporation of the Dendropanax morbiferus leaf extracts into the PU matrix. In addition, the nanofibers containing this leaf extract were confirmed to exhibit antibacterial properties against Staphylococcus aureus, suggesting the potential usefulness of nanofacial masks containing the Dendropanax morbiferus leaf extract.

Analysis and Quantitative Distribution of Glycosided Flavonoids in Citruses and Korean Chung-pi (감귤류와 한국산 청피에 함유된 Glycosided Flavonoids의 분석과 정량적 분포)

  • Baik, S.O.;Bock, J.Y.;Chun, H.J.;Jeong, S.I.;Baek, S.H.;Oh, H.B.;Kim, I.K.
    • Analytical Science and Technology
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    • v.14 no.4
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    • pp.340-348
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    • 2001
  • Glycosided flabonoids (naringenin, naringin, narirutin, hesperidin, and hesperetin) in Citrus and Korea Chung-pi were isolated and analyzed with HPLC, GC-mass, UV and high resolution NMR. Contents of glycosided flavonoids were compared according to kinds of Citrus and fruit ripening periods. Major compound of Korean Chung-pi was hesperidin and minors were narirutin and hesperetin. Major compounds of Gisil were naringin, narirutin, naringenin and were narirutin, hesperidin in Gigak. Major compounds of milgam and orange were narirutin, hesperidin, and the contents of glycoside flavonoids decreased according to the age of maturity.

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Comparative Radical Scavenging and Nitric Oxide Production Capacity of Water Extracts of Acanthopanax divaricatus var, Albeofructus and Eleutherococcus divaricatus var. Chiisanensis (지리산오가피와 흰털오가피 열수추출물의 라디칼 제거 활성 및 대식세포에서의 산화질소 생성능 비교)

  • Kim, Dong-Min;Jin, Bora;Kim, Ji Yeon
    • Journal of Applied Biological Chemistry
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    • v.57 no.4
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    • pp.337-340
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    • 2014
  • In the present study, water extracts of Acanthopanax divaricatus var, albeofructus and Eleutherococcus divaricatus var. chiisanensis were prepared and their antioxidant and anti-inflammatory effects were compared. The water extract of Acanthopanax divaricatus var, albeofructus showed significantly higher activity in total polyphenol and flavonoid contents, while there were no significant differences in ABTS/DPPH radical scavenging activities and ferric reducing antioxidant power. However, the water extract of Eleutherococcus divaricatus var. chiisanensis at the highest dose showed significantly lower capacity in nitric oxide production than that of Acanthopanax divaricatus var, albeofructus. Taken together, there were differences between Acanthopanax divaricatus var, albeofructus and Eleutherococcus divaricatus var. chiisanensis in total polyphenol/flavonoid contents and anti-inflammatory effect, however, the antioxidant effect was almost similar.

Effects of different UV-8 levels on the growth, photosynthesis and pigments in cucumber(Cucumis sativus L.) (UV-B 강도 변화가 오이(Cucumis sativus L.)의 생장, 광합성 및 색소에 미치는 영향)

  • Kim, Hak-Yoon;Lee, In-Jung;Shin, Dong-Hyun;Kim, Kil-Ung
    • Journal of Life Science
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    • v.8 no.3
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    • pp.272-278
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    • 1998
  • To investigate the effects of different UV-B levels on plant growth, cucumber plants were subjected to three levels of biologically effective ultraviolet-B(UV--$B^{BE}$ radiation [daily dose : 0.03(No UV-B), 6.40(Low UV-B) and 11.30 (High UV-B) kJ $m^{-2}$, UV--$B^{BE}$] in the growth chambers for 3 weeks during the early growth period. High and low levels of UV-B irradiation drastically decreased both dry weight and leaf area, but increased specific leaf weight of cucumber. Plants subjected to UV-B resulted in 30% and 20% reduction of photosynthesis rate by high and low UV-B, respectively. However, respiration rate was not affected by the UV-B. With increasing UV-B intensity, total chlorophyll contents were decreased linearly, while the contents of flavonoid were increased linearly. These results suggest that the present levels of UV-B may affect the growth of cucumber plant compared with a UV-B-free condition. It is likely that the growth of cucumber will be affected by enhanced UV-B due to ozone depletion in the near future.

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Antioxidant and Antimicrobial Activities of Trypsin-treated Pinus densiflora Ethanol Extract (트립신 처리에 따른 적송잎 추출물의 항산화 활성 및 항균 효과)

  • Moon, Ki-Eun;Park, Kyo-Hyun;Lee, Beom Zoo;Kim, Bae-Hwan
    • Journal of Environmental Health Sciences
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    • v.44 no.3
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    • pp.293-300
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    • 2018
  • Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and $ABTS^+$ radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.

Analysis of Nutritional Components and Physiological Activity of Butternut Squash (Cucurbita moschata) by Drying Methods (건조방법을 달리한 땅콩호박의 영양성분 분석 및 생리활성 평가)

  • Sim, Wan-Sup;Kim, Ho-Joong;Ku, Su-Bin;Chae, Seon-Hee;Choi, Yong-Won;Men, Xiao;Park, Sung-Min;Lee, Ok-Hwan
    • The Korean Journal of Food And Nutrition
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    • v.33 no.1
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    • pp.91-97
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    • 2020
  • The purpose of this study was to provide the basic data of butternut squash (Cucurbita moschata) according to the drying methods. The proximate composition and chromaticity of freeze-dried and hot air-dried Cucurbita moschata powders were evaluated, and the antioxidant effects of these ethanolic extracts were analyzed by DPPH, ABTS, FRAP, reducing power activity, total phenolic, and flavonoid content. As a result, Cucurbita moschata was revealed to have high carbohydrate and crude protein contents, while crude ash and crude fat contents were relatively low. Also, it was confirmed that the hot air-dried Cucurbita moschata had lower L, a, and b values than the freeze-dried Cucurbita moschata. Additionally, as a result of analyzing the antioxidant activity of Cucurbita moschata ethanolic extracts, hot air-dried Cucurbita moschata ethanolic extract showed significantly superior antioxidant activity than freeze-dried Cucurbita moschata ethanolic extract. Also, hot air-dried Cucurbita moschata ethanolic extract was revealed to have a higher polyphenol content, but slightly lower flavonoid content than freeze-dried Cucurbita moschata ethanolic extract. Based on the data from this study, further experiments on Cucurbita moschata material are necessary.

Changes in Phytochemical Content and Antiproliferative Activity of Germinated Geunnun and Ilpum Rice Varieties (큰눈벼와 일품벼의 발아에 의한 생리활성물질 함량 및 암세포 증식억제활성 변화)

  • Sung, Jeehye;Lee, Junsoo;Oh, Sea-Kwan;Lee, Jeom-Sig;Choi, Won-Seok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.7
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    • pp.1157-1161
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    • 2013
  • The purpose of this work was to measure the changes in ${\gamma}$-aminobutyric acid (GABA) and polyphenolic content of two different rice varieties (Geunnun and Ilpum), as well as the antiproliferative activities of both germinated brown rice varieties in cancer cells. The contents of GABA in Geunnun and Ilpum, especially in the bran of Ilpum increased significantly after germination. The content of polyphenol in Geunnun also increased after germination, but the contents of flavonoid in both varieties decreased after germination. A significant increase in the antiproliferative activity of both varieties on human lung and gastric cancer cell line was observed after germination.

Antioxidative and Anticancer Activities of Xanthium strumarium Extracts prepared from Different Parts (도꼬마리 부위별의 항산화 및 항암 활성)

  • Lee, Youn Ri
    • The Korean Journal of Food And Nutrition
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    • v.26 no.4
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    • pp.609-614
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    • 2013
  • This study was carried out in order to investigate the functional properties of methanol extracts from two parts (root and fruit) of Xanthium strumarium by means of measuring the contents of total polyphenols and flavonoid as well as determining $ABTS{\cdot}^+$, DPPH radical scavenging activity, hydroxyl radical scavenging activity (OH${\cdot}$) and anticancer activity. The examination of physiologically active substances in the two parts revealed that the Xanthium strumarium fruit had high total polyphenol, flavonoid contents, $ABTS{\cdot}^+$ DPPH and hydroxyl radical scavenging activity. The Xanthium strumarium fruit has higher activities of anticancer activities on prostate cell lines compared to other cancer cell lines.

Comparison of Bioactive Constituents and Biological Activities of Aronia, Blackcurrant, and Maquiberry (아로니아, 블랙커런트, 마키베리의 기능성 성분 및 생리활성 비교)

  • Chung, Hai-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.8
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    • pp.1122-1129
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    • 2016
  • In this study, bioactive constituents and biological activities of aronia, blackcurrant, and maquiberry were investigated. The 60% ethanol extracts were prepared and evaluated for total polyphenol, total flavonoid, and total anthocyanin contents. Biological activities, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, ferric reducing antioxidant power (FRAP), reducing power, tyrosinase inhibitory activity, and nitrite scavenging activity were also examined. Maquiberry showed the highest bioactive constituents as shown based on total polyphenol (73.66 mg GAE/g), total flavonoid (28.08 mg/g), and total anthocyanin (12.82 mg/g) contents compared to aronia and blackcurrant. Maquiberry also exhibited the highest DPPH and ABTS radical scavenging activity, FRAP, and reducing power at a concentration of 5 mg/mL, which were superior to those of ascorbic acid (a positive control). Therefore, maquiberry could be used as a potential source of antioxidants and functional food substances.