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http://dx.doi.org/10.5668/JEHS.2018.44.3.293

Antioxidant and Antimicrobial Activities of Trypsin-treated Pinus densiflora Ethanol Extract  

Moon, Ki-Eun (Department of Public Health, College of Natural Sciences, Keimyung University)
Park, Kyo-Hyun (Department of Public Health, College of Natural Sciences, Keimyung University)
Lee, Beom Zoo (CHEMLAND Co., Ltd.)
Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
Publication Information
Journal of Environmental Health Sciences / v.44, no.3, 2018 , pp. 293-300 More about this Journal
Abstract
Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and $ABTS^+$ radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.
Keywords
Antibacterial activity; Antioxidant; Pinus densiflora; Protease; Trypsin;
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Times Cited By KSCI : 6  (Citation Analysis)
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