• 한국식품영양학회지
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• 제33권2호
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• pp.210-214
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• 2020

In this study, polyphenol and flavonoid contents were measured, and DPPH, OH, H₂O₂ radical scavenging activity, and the α-amylase inhibitory activity were measured to study the antioxidant activity of 70% ethanol extract from Morinda citrifolia L. The polyphenol and flavonoid contents of noni 70% ethanol extract were 29.52 GAE/g and 12.48 CE/g, respectively. Also, the IC₅₀ values of DPPH, hydroxyl radical, and hydrogen peroxide scavenging activity of 70% ethanol extract from noni were 18.70 mg/mL, 26.45 mg/mL, and 35.67 mg/mL, respectively. Measurement of the α-amylase inhibitory activity of 70% ethanol extract from noni showed 45% inhibitory activity at 10 mg/mL.

• 한국식생활문화학회지
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• 제27권1호
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• pp.75-81
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• 2012

This study was conducted to investigate the physiological activity of acai berry (Euterpe oleracea Mart.) extracts from three different solvents (water, methanol, and ethanol). We measured total polyphenol and total flavonoid content, DPPH radical scavenging activity, nitrite scavenging activity, metal chelating effect, and reducing power. The extraction yield from water, methanol, and ethanol was 17.10, 9.50, and 37.51%, respectively. The highest total polyphenol content (10.54 mg/100 g) and total flavonoid contents (1.88 mg/100 g) was observed in water extract. DPPH radical scavenging activity was the highest in both water extract (72.03%) and methanol extract (74.79%) at levels of 5 mg/mL, which was similar to that of BHT (78.90%). Water extract yielded the highest metal chelating effect (92.54%) and reducing power (1.09) at levels of 5 mg/mL. Taken together, these findings suggest that extracts of acai berry can be used as functional food materials with antioxidative and nitrite scavenging activities.

• 한국식생활문화학회지
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• 제22권3호
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• pp.336-341
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• 2007

Antioxidant activity of Astragalus membranaceus, Polygonatum stenophyllum, Angelica gigas, Acanthopanax sessiliflorus and Angelica pubescens extracts cultivated in Jecheon prepared with different solvents were determined by 1,1-diphenyl-2-picrylhydrzyl(DPPH) radical scarvenging ability and thiocyanate method . The highest hydroxy radical scarvenging activity was shown in Acanthopanax sessiliflorus, while the lowest was in Polygonatum stenophyllum. Angelica gigas showed strong antioxidant activity by thiocyanate method. Methanol extracts and water extracts showed higher antioxidant activities than ethanol extracts. Angelica pubescens showed the highest polyphenol contents and Acanthopanax sessiliflorus showed the highest flavonoid contents. Direct correlation between the hydroxy radical scavenging activity and polyphenol contents or flavonoid contents was established by simple regression(r>0.8) in each solvent extracts. Among medicinal plants cultivated in Jecheon, Acanthopanax sessiliflorus and Angelica pubescens showed strong antioxidant activities. These results suggest that methanol or water extracts of several medicinal plant cultivated in Jecheon could be used as natural antioxidants.

• Preventive Nutrition and Food Science
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• 제21권2호
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• pp.110-116
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• 2016

This study was performed to evaluate and compare the antioxidant substance content and antioxidant activities of white (Superior) and colored (Hongyoung, Jayoung, Jasim, Seohong, and Jaseo) potatoes. The potatoes were extracted with 80% ethanol and were evaluated for the total polyphenol, flavonoid, and anthocyanin contents and for 1,1-diphenyl-2-picrylhydrazyl (DPPH)/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, reducing power, and ferrous metal ion chelating effect. The total polyphenol, flavonoid, and anthocyanin contents of Hongyoung and Jayoung were higher than white and other colored potatoes. All colored potato extracts, except for Jaseo and Seohong, showed higher ABTS radical scavenging activities than the general white potato extract. Hongyoung and Jayoung had the highest ABTS and DPPH radical scavenging activities. Optical density values for the reducing power of Jayoung and Jaseo at concentration of 2 mg/mL were 0.148 and 0.090, respectively. All colored potato extracts had lower ferrous metal ion chelating effect than the white potato. A significant (P<0.05) positive correlation was observed between total polyphenol content and total flavonoid content (r=0.919), anthocyanin content (r=0.992), and ABTS radical scavenging activity (r=0.897). Based on these results, this research may be useful in developing the Hongyoung and Jayoung cultivars with high antioxidant activities.

• 한국식품영양과학회지
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• 제41권6호
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• pp.739-744
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• 2012

본 연구는 자생분포가 높은 4가지 지역(경기, 강원, 전남 및 제주)에서 채집한 엉겅퀴를 에탄올 추출하여 총 폴리페놀과 총 플라보노이드 함량, DPPH 및 ABTS radical 소거능, 2가지 방법에 의한 환원력을 분석하여 새로운 자생식물 유래의 항산화제 소재개발에 있어 원물 최적화를 위하여 시행하였다. 총 폴리페놀 및 플라보노이드를 많이 함유하고 있을수록 4가지 항산화능의 평가 역시 효과적인 것으로 나타났다. 이는 총 폴리페놀 및 플라보노이드 함량과 항산화 활성의 유의적인 상관성이 있음을 확인할 수 있는 결과라 사료된 다. 엉겅퀴는 현재 잘 소비되는 식물자원은 아니지만 다양한 효능이 확인되고 있는 엉겅퀴를 이용한 소재 및 제품 개발을 위해서는 본 연구결과를 활용한 최적의 재배지역을 선정하고, 향후 재배기술 및 원물의 규격화를 위한 연구는 반드시 수행되어야 할 것으로 판단된다.

• KSBB Journal
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• 제31권2호
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• pp.100-104
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• 2016

Dolsan leaf mustard has various biological functions, including those in the immune system and cancer prevention. It contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the total phenolics contents and antioxidant activity in Dolsan leaf mustard pickle (DLMP) during storage. DLMP methanol extracts had the highest total polyphenol and flavonoid contents of $35.56{\pm}0.01GAEmg/g$ and $4.54{\pm}0.03QEmg/g$, respectively. The DPPH and ABTS radical scavenging activities in the DLMP methanol extracts showed the highest activities of 79.4 and 85.36%, respectively. The ferric reducing antioxidant power (FRAP) assay showed 2.24-3.82 mM $FeSO_4$ eq. (p<0.05) in DLMP extracts. Overall, storage at day 14 showed the highest antioxidant activity.

• 한국약용작물학회지
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• 제24권4호
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• pp.263-270
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• 2016

Background: In this study, microwave extraction was used, which is an effective method to extract useful bioactive substances as it requires low quantities of solvent and short time periods. The aim of this study was to determine the optimal extraction conditions for Agrimonia pilosa Ledeb. Methods and Results: The independent variables were ethanol concentration, microwave power, and extraction time, each of which had five levels. The dependent variables were total polyphenol and total flavonoid content, and DPPH radical scavenging activity. To determine the optimal extraction conditions for bioactive compounds, a response surface methodology was employed. Contour maps were generated from polynomial equations. The optimal conditions were then assumed by superimposing these contour maps. Based on the resulting graph, the optimal microwave extraction conditions for Agrimonia pilosa Ledeb were determined as 42 - 48% ethanol concentration, 240 - 280W microwave power, and 13 - 20 min of extraction time. Conclusions: Ethanol concentration had a significant effect on microwave extraction, in terms of total polyphenol and total flavonoid content, as well as DPPH radical scavenging activity. Microwave power and extraction time influenced the total polyphenol content, but not the total flavonoid content or the DPPH radical scavenging activity.

• Preventive Nutrition and Food Science
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• 제14권3호
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• pp.195-200
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• 2009

This study was carried out to investigate the biological activities of star anise extracts obtained from four different solvents (water, methanol, ethanol and chloroform) by measuring total polyphenol and flavonoid contents, DPPH radical scavenging, hydroxyl radical scavenging, nitrite scavenging activity and antimicrobial activity. The methanol extract showed the highest extraction yield, followed by ethanol, water and chloroform. The properties of the extracting solvents significantly affected the total polyphenol content. Methanol extracts contained more total polyphenols than any other extracts. The highest DPPH radical scavenging activity was found in methanol extract. The hydroxyl radical and nitrite scavenging activity were highest in methanol and ethanol extracts. In antimicrobial activity, water extract showed stronger activities than methanol and ethanol extract against Micrococcus luteus and Bacillus subtilis, and no inhibitory effects on Gram negative bacteria were found in all extracts at the concentration used.

• 한국약용작물학회지
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• 제21권4호
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• pp.255-261
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• 2013

The purpose of this study was to examined the antioxidant activities by water and 70% ethanol extract from durian (Durio zibethinus.) seed, sarcocarp and peel. Durian extract were studied for reducing sugar content, polyphenol content, superoxide dismutase (SOD) like activity, electron donating ability, nitrite scavenging ability, flavonoid content, hydroxy radical scavenging activity. Reducing sugar content were increased peel > sarcocarp > seed. Total polyphenol, flavonoid content, DPPH radical scavenging ability and SOD like activity were increased seed > peel > sarcocarp. Total polyphenol content was relatively high as $21.90{\pm}0.50mg/g$ in the ethanol extract of the seed. DPPH radical scavenging ability was relatively high as $62.08{\pm}2.63%$ in the water extract of the seed. Nitrite scavenging ability was no significant difference. Hydroxy radical scavenging activity was increased seed > peel > sarcocarp, was relatively high as $58.27{\pm}1.13%$ in the water extract of the seed.

• 한국식품과학회지
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• 제38권3호
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• pp.331-336
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• 2006

고온고압처리 조건에 따른 마늘의 이화학적 특성의 변화와 유용성분 변화를 확인하기 위하여 고온고압처리온도(110, 120, 130, 140 및 $150^{\circ}C$)와 시간(1, 2, 3, 4 및 5 시간)을 변수로 처리한 후 DPPH assay에 항산화활성$(IC_{50})$, 총 폴리페놀 함량, 총 플라보노이드 함량 및 5-HMF를 측정하였고 이틀의 상관관계를 분석하였다. 마늘은 항산화활성$(IC_{50})$은 고온고압처리 온도와 시간의 증가에 따라 증가하였으며, $150^{\circ}C$, 4시간 처리구에서 1.95 mg/g으로 가장 낮아 생마늘에 비해 약 50배 이상 증가하였다. 총 폴리페놀 함량은 고온고압처리 온도와 시간의 증가에 따라 증가하였으며, $140^{\circ}C$, 2시간에서 $18.16{\pm}0.51\;mg/g$으로 생마늘에 비해 약 7배정도 증가하였다, 총 플라보노이드 함량도 고온고압처리 온도와 시간의 증가에 따라 증가하였으며, $150^{\circ}C$, 1시간에서 $532.73{\pm}18.79\;{\mu}g/g$으로 생마늘보다 약 16배 높은 함량을 나타내었다. 5-HMF 함량은 110 및 $120^{\circ}C$에서는 변화가 작았지만 $130^{\circ}C$에서는 고온고압처리 시간의 증가에 따라 급격한 증가를 보였고 $150^{\circ}C$, 2시간에서 $7,765.9{\pm}359.6\;ppm$로 가장 높은 함량을 나타내었다. 통계분석 결과 고온고압처리온도와 시간이 항산화성, 폴리페놀, 플라보노이드 및 5-HMF 함량에 유의적으로 영향을 미치며(p<0.001), 처리 시간보다 온도가 더 많은 영향을 미치는 것으로 나타났다. 또한 항산화성, 폴리페놀, 플라보노이드 및 5-HMF 함량 간의 상관관계도 높게 나타났다(p<0.01).