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http://dx.doi.org/10.7783/KJMCS.2016.24.4.263

Prediction of Optimal Microwave Extraction Conditions for Functional Compounds from Agrimonia pilosa Ledeb Using Response Surface Methodology  

Park, Jong Jin (Department of Food Science and Technology, Kyungpook National University)
Lee, Won Young (Department of Food Science and Technology, Kyungpook National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.24, no.4, 2016 , pp. 263-270 More about this Journal
Abstract
Background: In this study, microwave extraction was used, which is an effective method to extract useful bioactive substances as it requires low quantities of solvent and short time periods. The aim of this study was to determine the optimal extraction conditions for Agrimonia pilosa Ledeb. Methods and Results: The independent variables were ethanol concentration, microwave power, and extraction time, each of which had five levels. The dependent variables were total polyphenol and total flavonoid content, and DPPH radical scavenging activity. To determine the optimal extraction conditions for bioactive compounds, a response surface methodology was employed. Contour maps were generated from polynomial equations. The optimal conditions were then assumed by superimposing these contour maps. Based on the resulting graph, the optimal microwave extraction conditions for Agrimonia pilosa Ledeb were determined as 42 - 48% ethanol concentration, 240 - 280W microwave power, and 13 - 20 min of extraction time. Conclusions: Ethanol concentration had a significant effect on microwave extraction, in terms of total polyphenol and total flavonoid content, as well as DPPH radical scavenging activity. Microwave power and extraction time influenced the total polyphenol content, but not the total flavonoid content or the DPPH radical scavenging activity.
Keywords
Agrimonia pilosa Ledeb; Extraction; Microwave; Optimization; Response Surface Methodology;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Giese J. (1992). Advances in microwave food processing. Food Technology. 46:118-123.
2 Kim DH, An BJ and Lee JY. (2011a). Whitening activities of the Agrimonia pilosa L. extracts. Journal of Applied Biological Chemistry. 54:284-289.   DOI
3 Kim TH, Kim JM, Baek JM, Kim TW, Kim DJ, Park JH and Choe M. (2011b). Antioxidant and whitening effects of Agrimonia pilosa Ledeb water extract. Korean Journal of Medicinal Crop Science. 19:177-184.   DOI
4 Kim YM. (2010). The inhibitory effect of Agrimonia pilosa Ledeb extract on allergic reaction. Korean Journal of Medicinal Crop Science. 18:398-404.
5 Kwon JH, Kim KG and Lee GD. (2000). Optimization of microwave-assisted extraction under atmospheric pressure condition for soluble ginseng components. Korean Journal of Food Science and Technology. 32:117-124.
6 Kwon MC, Kim CH, Kim HS, Lee SH, Chio GP, Park UY, You SG and Lee HY. (2007). Optimal extract condition for the enhancement of anticancer activities of Artemisia princeps Pampanini. Korean Journal of Medicinal Crop Science. 15:233-240.
7 Kwon YJ, Kim KH and Kim HK. (2002). Changes of total polyphenol content and antioxidant activity of Ligularia fischeri extracts with different microwave-assisted extraction conditions. Korean Journal of Food Preservation. 9:332-337.
8 Lee HJ, Do JR, Kwon JH and Kim HK. (2012). Optimization of Corni fructus extracts by response surface methodology. Journal of the Korean Society of Food Science and Nutrition. 41:390-395.   DOI
9 Lee WY, Choi SY, Lee BS, Park JS, Kim MJ and Oh SL. (2006). Optimization of extraction conditions from Omija (Schizandra chinensis Baillon) by response surface methodology. Korean Journal of Food Preservation. 13:252-258.
10 Min KJ, Song JW and Cha CG. (2008). The antioxidative and antitumor activity of extracts of Agrimonia pilosa. Journal of Food Hygiene and Safety. 23:149-156.
11 Pare JRJ, Belanger JMR and Stafford SS. (1994). Microwaveassisted process($MAP^{TM}$): A new tool for the analytical laboratory. Trends in Analytical Chemistry. 13:176-184.   DOI
12 Pei YH, Li X and Zhu TR. (1990). Studies on the structure of a new ellagic acid glycoside from the root sprouts of Agrimonia pilosa Ledeb. Acta Pharmaceutica Sinica. 25:798-800.
13 Schiffmann RF. (1992). Microwave processing in the U.S. food industry: Dielectric and ohmic sterilization. Food Technology. 46:50-56.
14 Singleton VL, Orthofer R and Lamuela-Raventos RM. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology. 299:152-178.   DOI
15 Teng H, Ghafoor K and Choi YH. (2009). Optimization of microwave-assisted extraction of active components from Chinese quince using response surface methodology. Journal of Applied Biological Chemistry. 52:694-701.
16 Theodore K and Panda T. (1995). Application of response surface methodology to evaluate the influence of temperature and initial pH on the production of ${\beta}$-1,3-glucanase and carboxymethylcellulase from Trichoderma harzianum. Enzyme and Microbial Technology. 17:1043-1049.   DOI
17 Wang L, Li D, Bao C, You J, Wang Z, Shi Y and Zhang H. (2008). Ultrasonic extraction and separation of anthraquinones from Rheum palmatum L. Ultrasonics Sonochemistry. 15:738-746.   DOI
18 Yoon JY, Lee SY, Jun HJ and Lee JY. (2012). Anti-aging effects of solvent fraction from Agrimonia pilosa L. extracts. Journal of Applied Biological Chemistry. 55:35-39.   DOI
19 Bae JH and Sohn MA. (2005). Effect of Agrimonia pilosa Ledeb extract on the growth of food-borne pathogens. The Korean Journal of Nutrition. 38:112-116.
20 Zhang HF, Yang XH and Wang Y. (2011). Microwave assisted extraction of secondary metabolites from plants: Current status and future directions. Trends in Food Science and Technology. 22:672-688.   DOI
21 Blois MS. (1958). Antioxidant determination by the use of a stable free radical. Nature. 181:1199-1200.   DOI
22 Chen L, Jin H, Ding L, Zhang H, Li J, Qu C and Zhang H. (2008). Dynamic microwave-assisted extraction of flavonoids from Herba Epimedii. Separation and Purification Technology. 59:50-57.   DOI
23 Gao Y, Zhou G, Chen J and Qin H. (2015). Determination of six flavonoids in Agrimonia pilosa Ledeb by HPLC. Food Science. 36:93-96.