• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.753-760
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• 2007

The diversity of Paenibacillus species was assessed in the rhizospheres of four cultivars of sorghum sown in Cerrado soil amended with two levels of nitrogen fertilizer(12 and 120 kg/ha). Two cultivars(IS 5322-C and IS 6320) demanded the higher amount of nitrogen to grow, whereas the other two(FBS 8701-9 and IPA 1011) did not. Using the DNA extracted from the rhizospheres, a Paenibacillus-specific PCR system based on the RNA polymerase gene(rpoB) was chosen for the molecular analyses. The resulting PCR products were separated into community fingerprints by DGGE and the results showed a clear distinction between cultivars. In addition, clone libraries were generated from the rpoB fragments of two cultivars(IPA 1011 and IS 5322-C) using both fertilization conditions, and 318 selected clones were sequenced. Analyzed sequences were grouped into 14 Paenibacillus species. A greater diversity of Paenibacillus species was observed in cultivar IPA 1011 compared with cultivar IS 5322-C. Moreover, statistical analyses of the sequences showed that the bacterial diversity was more influenced by cultivar type than nitrogen fertilization, corroborating the DGGE results. Thus, the sorghum cultivar type was the overriding determinative factor that influenced the community structures of the Paenibacillus communities in the habitats investigated.

• Journal of Korean Neurosurgical Society
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• 제60권5호
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• pp.550-559
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• 2017

Objective : Subsidence is a frequent complication of anterior cervical discectomy and fusion. Postoperative segmental micromotion, thought to be a causative factor of subsidence, has been speculated to increase with uncinate process resection area (UPR). To evaluate the effect of UPR on micro-motion, we designed a method to measure UPR area based on pre- and postoperative computed tomography images and analyzed the relationship between UPR and subsidence as a proxy of micro-motion. Methods : We retrospectively collected clinical and radiological data from January 2011 to June 2016. A total of 38 patients (53 segments) were included. All procedures included bilateral UPR and anterior plate fixation. UPR area was evaluated with reformatted coronal computer tomography images. To reduce level-related bias, we converted UPR area to the proportion of UPR to the pre-operative UP area (pUPR). Results : Subsidence occurred in 18 segments (34%) and positively correlated with right-side pUPR, left-side pUPR, and the sum of bilateral pUPR (sum pUPR) (R=0.310, 301, 364; p=0.024, 0.029, 0.007, respectively). Multiple linear regression analysis revealed that subsidence could be estimated with the following formula : $subsidence=1.522+2.7{\times}sum\;pUPR$($R^2=0.133$, p=0.007). Receiver-operating characteristic analysis determined that sum $pUPR{\geq}0.38$ could serve as a threshold for significantly increased risk of subsidence (p=0.005, area under curve=0.737, sensitivity=94%, specificity=51%). This threshold was confirmed by logistic regression analysis for subsidence (p=0.009, odds ratio=8.471). Conclusion : The UPR measurement method confirmed that UPR was correlated with subsidence. Particularly when the sum of pUPR is ${\geq}38%$, the possibility of subsidence increased.

• Knee surgery & related research
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• 제30권4호
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• pp.341-347
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• 2018

Purpose: Tunnel widening following anterior cruciate ligament (ACL) reconstruction is commonly observed. Graft micromotion is an important contributing factor. Unlike fixed-loop devices that require a turning space, adjustable-loop devices fit the graft snugly in the tunnel. The purpose of this study is to compare tunnel widening between these devices. Our hypothesis is that the adjustable-loop device will create lesser tunnel widening. Materials and Methods: Ninety-eight patients underwent ACL reconstruction from January 2013 to December 2014. An adjustable-loop device was used in 54 patients (group 1) and a fixed-loop device was used in 44 patients (group 2). Maximum tunnel widening at 1 year was measured by the L'Insalata's method. Functional outcome was measured at 2-year follow-up. Results: The mean widening was 4.37 mm (standard deviation [SD], 2.01) in group 1 and 4.09 mm (SD, 1.98) in group 2 (p=0.511). The average International Knee Documentation Committee score was 78.40 (SD, 9.99) in group 1 and 77.11 (SD, 12.31) in group 2 (p=0.563). The average Tegner-Lysholm score was 87.25 (SD, 3.97) in group 1 and 87.29 in group 2 (SD, 4.36) (p=0.987). There was no significant difference in tunnel widening and functional outcome between the groups. Conclusions: The adjustable-loop device did not decrease the amount of tunnel widening when compared to the fixed-loop device. There was no significant difference in outcome between the two fixation devices. Level of Evidence: Level 3, Retrospective Cohort.

• Archives of Aesthetic Plastic Surgery
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• 제23권1호
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• pp.1-10
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• 2017

Background Creating a natural-looking umbilicus during closure of the donor-site in abdominally based free flap breast reconstruction is a factor of satisfaction for both the patient and surgeon. We present a simple method of umbilical transposition that results in an aesthetic, natural-looking umbilicus. Methods From March 2011 to November 2014, fifty three consecutive female patients received abdominal flap breast reconstruction. Twenty patients (from March 2011 to February 2013) underwent umbilical transposition through a cross like incision in the abdominal flap, with fascial fixation sutures but no dermal flaps. Thirty three patients (March 2013 to November 2014) received umbilical transposition in the following method. An oval-shaped incision is made at the location of the new umbilicus on the abdominal flap. This oval is deepithelialized, and full-thickness incisions are made at the 2, 6, and 10 o'clock directions to create three triangular dermal flaps. These are pulled down to the abdominal fascia using sutures that pass through the umbilical stalk and the abdominal fascia at the 3, 9, and 12 o'clock directions. This results in an umbilical stalk lined with dermal flaps, creates a natural periumbilical concavity, and anchors the abdominal flap inward to minimize tension. The cranial flap enhances superior hooding. Results Patient and surgeon satisfaction, surveyed 2 months after surgery with a satisfaction scoring system, were higher in the dermal flap group. Conclusions The technique using three dermal flaps in an oval skin incision is simple, relatively easy to learn, and results in an aesthetic, natural-looking umbilicus.

• 가정∙방문간호학회지
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• 제28권2호
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• pp.178-185
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• 2021

Purpose: In this study, we aimed to explore the risk factors for catheter-associated urinary tract infections (CAUTI) in patients receiving home care nursing. Methods: A total of 117 participants registered for home care nursing provided by the University of C hospital in Seoul were included in the study. Data were based on a survey and urine examination results from June to July 2018. Results: Asymptomatic bacteriuria was identified in 96 (82%) patients. Age, sex, activity, diabetes mellitus, mental status, presence of other diseases, catheter material type, catheter size and fixation, hand washing (care giver), gloving (care giver), perineal care, and bladder irrigation were not recognized as risk factors for CAUTI. Age and catheterization duration were associated with CAUTI. Conclusions: Old age was found to be a risk factor for CAUTI (p=0.048). CAUTI incidence decreased as catheter use exceeded 70 months (p=0.028).

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1040-1050
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• 2024

To isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas aeruginosa. Bioassay results demonstrated that this strain possessed robust antagonistic abilities, effectively inhibiting V. dahliae spore germination and mycelial growth. Furthermore, P. aeruginosa ZL6 exhibited high temperature resistance (42℃), nitrogen fixation, and phosphorus removal activities. Pot experiments revealed that P. aeruginosa ZL6 fermentation broth treatment achieved a 47.72% biological control effect compared to the control group. Through activity tracking and protein mass spectrometry identification, a neutral metalloproteinase (Nml) was hypothesized as the main virulence factor. The mutant strain ZL6ߡNml exhibited a significant reduction in its ability to inhibit cotton Verticillium wilt compared to the strain P. aeruginosa ZL6. While the inhibitory activities could be partially restored by a complementation of nml gene in the mutant strain ZL6CMߡNml. This research provides a theoretical foundation for the future development and application of biogas residue as biocontrol agents against Verticillium wilt and as biological preservatives for agricultural products. Additionally, this study presents a novel approach for mitigating the substantial amount of biogas residue generated from kitchen waste fermentation.

• Hip & pelvis
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• 제34권2호
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• pp.106-114
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• 2022

Purpose: While initial fixation using a press-fit of the acetabular cup is critical for the durability of the component, restoration of the hip center is regarded as an attributable factor for implant survival and successful outcome. In protrusio acetabuli (PA), obtaining both restoration of the hip center and the press-fit of the acetabular cup simultaneously might be difficult during total hip arthroplasty (THA). We tested the hypothesis that use of a medialized cup, if press-fitted, will not result in compromise of the implant stability and outcome after cementless THA of PA. Materials and Methods: A total of 26 cementless THAs of 22 patients with PA were reviewed. During THA, press-fit of the cup was prioritized rather than hip center restoration. A press-fit was obtained in 24 hips. A pressfit could not be obtained in the two remaining hips; therefore, reinforcement acetabular components were used. Restoration of the hip center was achieved in 17 cups; 15 primary cups and two reinforcement components; it was medialized in nine cups. Implant stability and modified Harris hip score (mHHS) between the two groups were compared at a mean follow-up of 5.1 years (range, 2-16 years). Results: Twenty-six cups; 17 restored cups and nine medialized press-fitted cups, remained stable at the latest follow-up. A similar final mHHS was observed between the restored group and the medialized group (83.6±12.1 vs 83.8±10.4, P=0.786). Conclusion: Implant stability and favorable results were obtained by press-fitted cups, irrespective of hip center restoration. THA in PA patients showed promising clinical and radiological results.

• 대한골관절종양학회지
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• 제17권2호
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• pp.58-64
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• 2011

목적: 단순 골낭종의 병적 골절 환자를 대상으로 단순 골낭종의 관해에 미치는 예후 인자에 대하여 알아보고자 하였다. 대상 및 방법: 2001년부터 2010년까지 본원에 내원한 단순 골낭종을 동반한 병적 골절 환자 13명을 대상으로 하였으며, 평균 추시 기간은 26개월(3-90개월)이었다. 성별은 남자 11명, 여자 2명이었으며, 평균 나이는 10.2세(6-16세)였다. 발생 부위는 근위 상완골 9명, 상완골 간부 2명, 근위 대퇴골 1명, 근위 경골 1명이었다. 단순 골낭종의 병적 골절에 대하여 보존적 치료로 단순 석고 고정 후 골절 유합을 시도하였으며, 1명은 일차적으로 골이식 및 관헐적 정복술과 내고정술을 시행하였다. 5명의 환자에서 치료 경과 중 스테로이드 주사를 시행하였으며, 2명에서 골이식을 시행하였다. 병적 골절 치료 중의 단순 골낭종의 변화 및 연령, 낭종의 크기, 성장판 침범 정도에 따른 단순 골낭종의 예후 인자에 대해 분석하였다. 결과: 병적 골절 후 전례에서 평균 8.2개월에 골유합을 얻었으며, 13명의 환자 중 4명(31%)에서 골낭종의 완전 관해를 보였다. 발병 나이에 따른 단순 골낭종 관해의 통계적 차이는 없었지만(p=0.42), 낭종의 크기가 클수록, 성장판에 근접한 경우 관해를 얻기가 어려웠다(p=0.05, p=0.03). 결론: 단순 골낭종의 병적 골절에서 관해가 이루어질 수 있으나, 낭종의 크기가 크거나 성장판에 근접한 경우에는 좀 더 적극적인 치료가 필요할 것으로 보인다.

• 대한공업교육학회지
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• 제33권2호
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• pp.120-136
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• 2008

로봇과 같은 물리적인 객체와 교육용 프로그래밍 언어를 통합한 실체적 프로그래밍 지원 도구는 일반적인 문제해결력 신장 뿐 아니라, 창의적 사고 발현에 유용한 도구이다. 즉, 학습자가 설계한 문제해결과정을 가상의 시뮬레이션이 아닌 물리적 객체를 통해 시뮬레이션 하는 경험을 제공해 줌으로써, 사전지식이나 문제해결과정에 대한 고정관념을 최소화할 수 있는 기회를 제공한다. 이러한 경험은 새로운 해법을 꾸준히 탐색하고 능동적으로 환경을 변경하고자 하는 창의적 사고 유발에 효과적이다. 따라서 본 연구에서는 로봇을 활용한 프로그래밍 교수 학습 프로그램을 개발하여 이를 대학교 프로그래밍 입문 과정의 초보 학습자들에게 적용하였고, 로봇 활용 프로그래밍 학습이 학습자들의 창의적 문제해결성향 증진에 유의미한 영향을 주었음을 확인하였다. 특히 창의적 문제해결성향을 구성하는 3가지 하위요인 중 인지적 요인이 통제집단에 비해 유의미하게 높게 나타났다. 인지적 요인은 일반적인 문제해결력 뿐 아니라 창의성을 설명하는 문제인지 및 표상, 확산적 사고 등의 개념을 포함하고 있다. 따라서 이러한 결과는 로봇 활용 실체적 프로그래밍 학습이 학습자의 창의적 문제해결력 향상에 긍정적인 요인으로 작용할 수 있음을 의미한다. 또한 집단별 사전 사후 창의적 문제해결성향 향상 정도를 분석한 결과, 로봇 활용 프로그래밍 학습 집단은 인지적 요인, 정의적 요인, 지식구성 요인 모두에서 유의미한 향상을 보였다. 이러한 결과는 로봇 프로그래밍 학습을 위한 교수 전략들이 문제해결력 뿐 아니라, 동기와 같은 정의적 요인 모두에 유의미한 영향을 끼친 것으로 해석할 수 있다.

• 한국학교수학회논문집
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• 제17권4호
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• pp.507-539
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• 2014

문자 기호 사용으로 대표되는 대수는 수학 전반에 그 영향력을 행사하는 중요한 도구로 자리매김하게 되었다. 이러한 대수를 적절히 활용하기 위해서는 무엇보다 주어진 문제 상황을 적합한 대수적 표상으로 전환하는 작업이 요구된다. 그러나 문장제에 관한 몇 가지 연구로부터 이러한 전환의 어려움이 보고되고 있다. 본 연구에서는 학생들이 주어진 문장 표상과 기하 표상 각각을 대수적 표상으로 전환 및 정교화하는 과정을 살펴보는데 초점을 두었다. 중학교 1학년 학생 29명을 대상으로 하여 문장으로 기술된 상황과 도형 표현이 추가된 상황을 제시하고 각 상황에서 요구하는 바를 대수적 표상으로 전환하는 능력을 조사한 결과 도형 표현을 대수적 표상으로 전환하는 하나의 문항을 제외하고 나머지 3개의 문항에서 10% 내외의 학생이 부적절한 응답을 하였다. 나아가 그 중 임의 추출한 네 명을 개별 면담함으로써 사고 특징 및 대수적 표상 정교화를 돕는 요인을 조사하였다. 그 결과, 대수 표상 정교화 과정은 급진적이 아닌 점진적 개선 과정임을 확인할 수 있었다. 그리고 대수적 표상 정교화를 요하는 문제에 대해 문제 요구 사항에 대한 오해가 있을 수 있음을 확인할 수 있었다. 또한 자신의 대수적 표상에 대한 설명과 구체적 수치 상황 제시가 정교화에 도움이 되는 요인으로 작용하는 것을 목격하였으며, 아울러 정교화의 경험은 전이력을 가질 수 있음을 확인할 수 있었다. 한편, 변수에 관한 오개념 등식 설정에 고착된 사고는 표상 전환의 방해 요소로 작용할 수 있음을 알 수 있었다. 이러한 결과로부터 대수적 표상 전환 및 정교화를 돕기 위한 몇 가지 교육적 시사점을 도출하였다.