• Journal of Marine Bioscience and Biotechnology
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• v.2 no.1
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• pp.1-10
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• 2007

Coral reefs are among the most dynamic and various ecosystems on tropical ecosystem. They provide a large number of important ecosystem services. Despite their importance, they appear to be one of the most susceptible marine ecosystems. Dramatic decreasing of coral reefs has been reported from every part of the world. Indonesia contains 18% of coral reefs of world's total. Unfortunately the status of coral reefs in Indonesia is already in critical and poor condition. Coral reefs communities in Indonesia are subjected to a variety of environmental disturbance. Threats to Indonesia's coral reefs resources can be divided into two main types: anthropogenic and natural disturbances. The major anthropogenic disturbances on coral reefs in Indonesia are destructive fishing, pollution, coastal development, mining and harvesting live fish and coral, tourism. The natural disturbances such as cyclones, volcanic eruptions, earthquakes, tsunami and predator also contribute to coral reefs destruction in Indonesia. In my paper I tried to compare between natural and anthropogenic disturbances on coral reefs in Indonesia and raised these questions: (i) how the natural disturbances differ from anthropogenic area (ii) which type of disturbances has caused the greatest impact on coral reef ecosystem. My finding is that both of anthropogenic and natural disturbances give major impact on coral reefs in Indonesia. The important issue here is coral reef resilience could be disturbed by synergistic effects between various anthropogenic and natural disturbances. This phenomenon has significant conservation and management implication. The appropriate management should be conducted to protect coral reefs ecosystem in Indonesia. Mangrove management will succeed only when local people are involved and get sustainable benefits from mangrove ecosystem. Community based management and Integrated Coastal Zone Management (ICZM) are type of management that can be applied on coral reef ecosystems in Indonesia.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7479-7486
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• 2014

Background: Colorectal cancer (CRC) is one of the most common cancers worldwide. This study aimed to investigate the interaction between the presence of a polymorphism of the XRCC1 gene and known risk factors for colorectal cancer in Thailand. Materials and Methods: A hospital-based case-control study was conducted in Thailand. The participants were 230 histologically confirmed new cases and 230 controls matched by sex and age and recruited from the same hospital. Information about demographic characteristics, life style, and dietary habits was collected using structured interviews, and blood samples were taken which were used for the detection of a homozygous and heterozygous polymorphisms of XRCC1. Associations were assessed using multiple conditional logistic regression. Results: In the univariate analysis, factors found to be significantly associated with an increased risk for CRC were the presence of the XRCC1 AA homozygote (OR= 4.95; 95% CI: 1.99-12.3), a first degree family history of cancer (OR= 1.74; 95% CI: 1.18-2.58), and a high frequency of pork consumption (OR= 1.49; 95% CI: 1.00-2.21). Intakes of fish fruit and vegetables appeared to be protective factors, but the associations were not statistically significant. In the multivariate analysis only the XRCC1 AA homozygote polymorphism and a family history of cancer emerged as risk factors (OR= 4.96; 95% CI: 1.90- 12.95 and OR=1.80; 95% CI: 1.18-2.72, respectively). Conclusions: While the XRCC1 AA homozygote and a family history of cancer were found to be associated with an increased risk of CRC, none of the dietary intake variables were clearly identified as risk or protective factors. There is a need for further research to determine the reasons for this.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.2111-2114
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• 2016

Opisthorchiasis is a health problem in Thailand particularly in northeast and north regions where have been reported the highest of cholangiocarcinoma. Active surveillance is required, therefore a cross-sectional surveyed was conducted in Nong Bunnak sub-district of Nakhon Ratchasima province, Thailand. A total of 367 participants were selected by multistage sampling from 5 villages located near natural water resources. Participants completed a predesigned questionnaire containing behavior questions regarding liver fluke disease, covering reliability and validity knowledge (Kuder-Richardon-20) = 0.80, attitude and practice (Cronbach's alpha coefficient) = 0.82 and 0.79, respectively. Descriptive statistics included frequencies, percentages, means, and standard deviations. The majority of the participants were female (58.3%), age group between 21-30 years old (42.5%), with primary school education (59.9%), occupation in agriculture (38.1%), and married (80.9%). They had past histories of raw fish consumption (88.3%), stool examination (1.4%), anti-parasite medication used (4.6%). Heads of villages, village health volunteers, television, and village newstations were the main sources for disease information. Participants had a moderate level of behavior regarding liver fluke disease. The mean scored of knowledge regarding liver fluke life cycle, transmission, severities, treatment, prevention and control was 10.9 (SD=0.5), most of them had a moderate level, 95.1%. The mean score for attitude regarding liver fluke prevention and control was 45.7 (SD=9.7), and for practice was 30.6 (SD=10.5). Participants had a moderate level of attitude and practice, 94.5% and 47.7, respectively. This study indicates that health education is required in this community including stool examination for liver fluke as further active surveillance screening.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3295-3300
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• 2016

Background: Different types of cancer exhibit abnormalities in cell cycle regulators. The murine double minute-2(MDM2) cell cycle regulator is a proto-oncogene that negatively regulates the P53 tumour suppressor gene. Surface epithelial tumours constitute approximately two thirds of ovarian neoplasms. Each histologic type can be classified as benign, borderline and malignant. This study aimed to examine immunohistochemical expression of the MDM2 protein in ovarian serous and mucinous epithelial tumours (benign, borderline and malignant). Materials and Methods: This study included forty five ovarian tumours, subdivided into fifteen cystadenomas (5 serous and 10 mucinous), fifteen borderline tumours (11 serous and 4 mucinous) and fifteen cystadenocarcinomas (9 serous and 6 mucinous). Paraffin sections were stained with haematoxylin and eosin for histopathologic study, and with mouse monoclonal anti-MDM2 antibody for immunohistochemistry. Results: MDM2 positivity was detected in 28.9% of the studied ovarian tumours. All benign tumours were negative and positivity was significantly higher in malignant than borderline tumours (P value of chi-square test =0.000). Significantly, all MDM2 positive mucinous tumours were malignant with no positive mucinous borderline tumours. Malignant tumours showed positive MDM2 expression in 83.3% of mucinous type and in 55.6% of serous type. Borderline serous tumours showed negative MDM2 in 72.7% of cases (P value of Z test =0.04). Conclusions: Alterations in the expression of the cell cycle regulator (MDM2) occur early in the process of tumourigenesis in serous and mucinous ovarian tumours. We suggest that MDM2 may be used in those tumours as a marker for risk stratification and identification of cases with cancer development and progression. We recommend further studies on MDM2 immunohistochemistry, in conjunction with adjuvant methods as DNA ploidy and FISH gene amplification, focusing on the mucinous tumours and differentiating between the three tumour categories, benign, borderline and malignant.

• Korean Journal of Microbiology
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• v.35 no.3
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• pp.242-247
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• 1999

This sludy was conducted to investigate the change of bacterial co~munities with season and depth in Lake Soyang. Korea, using fluorescence in situ hybridization (FISH). The oligonucleotide probes used in this study were EUB338, ALF I b. GAM42a, and CF, The percentage of h e Proleobacteria a $\alpha$-group ranged from 0.70 to 33% the $\beta$-group from 1.0 to 26% they -group from 2.4 Lo 37% and Cytophagn and Flavobactefin groups from 4.7 to 24% duing the study period (April Lo November, 1998). They $\gamma$-group was dominant in spring when Asterionella was dominant. and a $\alpha$-group was dominant in summer when the organic content was low and Dinobryon was dominant. However, a specific group was not dominant in ？dl when cyanobacteria group was dominant and the ratio of eubacleria to total bacteria was very low. Therefore, the bacterial communities in Lake Soyang changed with season and depth, which seems to be associated with the telnporal succession of phytoplanlaons.

• Preventive Nutrition and Food Science
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• v.7 no.2
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• pp.201-206
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• 2002

Findings from European and American studies have indicated such health behaviors as smoking, drinking, lack of exercise, and insufficient intake of grains, fruits and vegetables as risk factors for hypertension. However, because dietary pattern and health behaviors of Korean differ from people of other countries, the risk factors for Koreans could be different. Therefore, the aim of this study was to identify food consumption patterns and health behavior characteristics of Korean hypertensive adults. Data on food consumptions and other health behaviors such as smoking, drinking, and exercise together with development of hypertension were collected from 597 persons aged 20 to 69 years in a local community. The first stage of analysis utilized cluster analysis to aggregate individuals into different health behavior and food consumption groups. Four health behavior groups were characterized by passive cluster, smoker cluster, fitness cluster and drinker cluster. Food consumption patterns of the subjects were also aggregated into 4 different clusters: dairy cluster, grain & vegetable cluster, fruit cluster, and fish & meat cluster. Then univariate analysis was followed to identify the variables associated with hypertension. The final stage of analysis was the identification of the relative importance of the variables selected from the univariate analysis on hypertension, using multiple logistic analysis. The results showed that heavy drinking was the most significant health behavior associated hypertension, which was similar to the findings in European and American studies. However, unlike the findings from foreign studies, grain and vegetable consumption appeared to be a risk factor for hypertension. A possible reason for the contradictory results between Korean and Western studies may be the dependence of Koreans on white rice as the major staple food, and/or the frequent consumption of salted vegetables, rather than fresh vegetables as is customary in Europe and America.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.43 no.4
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• pp.241-250
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• 2007

Fishes were caught by a bottom trawl in the Yeo-ja bay of Korea by every three month(March, June, September and December) and seasonal variation of abundance and species composition were studied in 2005. The catches were 1,187kg and were composed of 115 species with 20 hauls by 5 stations. The dominant species caught were Charybdis bimcaulata, Crangon hakodatei, Parapenaeus fissurus, Oratosguilla oratoria, and Thryssa purava. One hundred fifty species were identified as fish(38%), crustacea(20%), mollusca(4%) and others(38%) which including of Asterias amurensis. The amount of species was high in June and September and low in March and December. The diversity index(H') was about 1.2－2.5 and station similarity of fishes caught among the community was 0.009－0.230.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.4
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• pp.248-254
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• 1991

The demersal fishes of Asan Bay were collected by an otter trawl in August 1990 to determine optimum sample size for the analysis of community structure. A total of 17 species comprising 957 individuals and 21,840 grams in biomass was collected. Predominant species were Cynoglossus joyneri, Thrissa koreana, Hexagrammos otakii and Johnius belengerii. Coefficients of variation for fish numbers in ten replicate tows ranged from 2.2 to $385.1\%$ for four abundant species and from 52.2 to $162.0\%$ when all species were considered. The cumulative number of species increased rapidly until 4 hauls, and less than 1 species per haul in average was added thereafter. The cumulative diversity index reached nearly an asymptote value when three of samples were combined. Variance in the number of individuals diminished as the sample size increased. The ratio of variance to mean numbers (dispersion index) was not significantly different from the unity when first 4-haul samples were combined. Four of 20-minute trawl haul are proposed to be a proper sampling size for the unbiased estimation of abundance in the study area.

• Animal cells and systems
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• v.16 no.2
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• pp.162-171
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• 2012

We investigated food webs of a $Salix$ $subfragilis$-dominated wetland in the Janghang wetland in the Hangang estuary, which is very close to the Demilitarized Zone, along the west coast of Korea. Our study focused on understanding sesarmine crab ($Sesarma$ $dehaani$)-related food webs in a $S.$ $subfragilis$ forest. For our study, we used carbon and nitrogen stable isotopes and fatty acid biomarkers. We collected samples of plants, animals, and detrital sediment from four quadrats ($5{\times}5m^2$) set in the $S.$ $subfragilis$ community. Samples were collected from September 2006 to June 2009, except during the winter hibernation period of $S.$ $dehaani$. In the wet season, the sediment showed relatively high ${\delta}^{13}C$ and low ${\delta}^{15}N$ signatures compared with relatively low ${\delta}13C$ and high ${\delta}15N$ signatures in the dry season. Mature $S.$ $dehaani$ appeared to feed on fresh leaves and other carbon sources, such as immature individuals or fish, in addition to detrital sediment, which appeared to be the main carbon source for immature crabs. Principal component analysis of fatty acid biomarkers of $S.$ $dehaani$ showed a clear difference between immature individuals (10-30 mm) and mature ones (larger than 30 mm), indicating that the main food source for immature crabs was detrital sediment, whereas mature crabs foraged plants in addition to consuming detrital sediment. On the basis of our results from stable isotope and fatty acid analyses, mature $S.$ $dehaani$ appeared to feed on detrital sediment and fresh leaves of $S.$ $subfragilis$ in summer in addition to engaging in cannibalism of immature individuals.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1826-1835
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• 2008

An autotrophic denitrification reactor (ADR-l) and a heterotrophic denitrification reactor (HDR-2) were operated to remove nitrate and nitrite in an anoxic environment in raw sewage. The $NO_3$-N removal rate of ADR-l was shown to range from 52.8% to 78.7%, which was higher than the $NO_3$-N removal rate of HDR-2. Specific denitrification rates (SDNR) of ADR-l and HDR-2 were 3.0 to 4.0 and 1.1 to $1.2\;mgNO_3$-N/gVSS/h, respectively. From results of restriction fragment length polymorphism (RFLP) of the 16S rRNA gene, Aquaspirillum metamorphum, Alcaligenes defragrans, and Azoarcus sp. were $\beta$-Proteobacteria that are affiliated with denitritying bacteria in the ADR-l. Specifically, Thiobacillus denitrificans was detected as an autotrophic denitrification bacteria. In HDR-2, the $\beta$-Proteobacteria such as Denitritying-Fe-oxidizing bacteria, Alcaligenes defragrans, Acidovorax sp., Azoarcus denitrificans, and Aquaspirillum metamorphum were the main bacteria related to denitrifying bacteria. The $\beta$-and $\alpha$-Proteobacteria were the important bacterial groups in ADR-l, whereas the $\beta$-Proteobacteria were the main bacterial group in HDR-2 based on results of fluorescent in situ hybridization (FISH). The number of Thiobacillus denitrificans increased in ADR-l during the operation period but not in HRD-2. Overall, the data presented here demonstrate that many heterotrophic denitritying bacteria coexisted with autotrophic denitrifying bacteria such as Thiobacillus denitrificans for nitrate removal in ADR-l. On the other hand, only heterotrophic denitritying bacteria were identified as dominant bacterial groups in HDR-2. Our research may provide a foundation for the complete nitrate removal in raw sewage of low-COD concentration under anoxic condition without any external organic carbon or the requirement of post-treatment.