• Environmental Analysis Health and Toxicology
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• v.11 no.1_2
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• pp.11-17
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• 1996

The purpose of this experiment is to estabilish the sensitive and specific ELISA (enzyme linked immunosorbent assay) system for the detection of fish metallothionein (MT). Silver carp were injected with CA of 1-8mg/kg body wt. 4 times during 10 days. Silver carp was very tolerant species to CA. Cd induced MT in liver was seperated and purified by gel filtration chromatography and ion exchange chromatography and identified by spectrophotometry, native gel electrophoresis and western blot analysis. The rabbit antiserum was produced by immunizing rabbit with lyophilized MT, and the competitive ELISA system was estabilished for the detection of fish MT. In the present ELISA system, the detection limit was about 33 ng/ml. When this ELISA system was employed to determine the MT level in the supernatant sample of fish liver homogenate, the reaction curve showed a good parallel corelationship with the calibration curve over a certain dilution range. The results indicate that the competitive ELISA can be a useful tool for the detection of fish MT in the toxicological study and the evaluation of water pollution.

• Annual Conference of KIPS
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• 2016.10a
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• pp.510-513
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• 2016

Detecting of the object in image processing is substantial but it depends on the object itself and the environment. An object can be detected either by its shape or color. Color is an essential for pattern recognition and computer vision. It is an attractive feature because of its simplicity and its robustness to scale changes and to detect the positions of the object. Generally, color of an object depends on its characteristics of the perceiving eye and brain. Physically, objects can be said to have color because of the light leaving their surfaces. Here, we conducted experiment in the aquarium fish tank. Different color of fish robots are mimic the natural swim of fish. Unfortunately, in the underwater medium, the colors are modified by attenuation and difficult to identify the color for moving objects. We consider the fish motion as a moving object and coordinates are found at every instinct of the aquarium to detect the position of the fish robot using OpenCV color detection. In this paper, we proposed to identify the position of the fish robot by their color and use the position data to control the fish robot gathering in one point in the fish tank through serial communication using RF module. It was verified by the performance test of detecting the position of the fish robot.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.508-513
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• 2008

Erythromycin has been used to treat Streptococosis, Edwardsiel1osis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electro spray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, $87.6{\pm}5.0%$; black rockfish, $87.2{\pm}6.4%$; eel, $85.2{\pm}4.8%$; and rainbow trout, $86.0{\pm}6.2%$. In the established method in this study, the correlation of coefficient values ($R^2$) of erythromycin calibration curve (n=11) was 0.9998.

• Journal of fish pathology
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• v.12 no.1
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• pp.66-69
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• 1999

Occurrences of red sea bream iridovirus disease (RSIVD) in cultured marine fishes were investigated. The infection was detected by the polymerase chain reaction (PCR) used to amplify the red sea bream iridovirus (RSIV). The RSIV infection was widely distributed in fish culture farm around the south coastal area of the Korean peninsula.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.22 no.4
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• pp.98-103
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• 1986

The passive acoustic system only has generally used in fish detection. But the passive acoustic system has not been tried in fishing since Freytag has proposed a possibilities of the passive detection of fishes in 1963. This paper describes the .feasible aspects of fish detection by listening of the sound they make. The passive acoustic system accompanied the active acoustic system may expand the range of detection and compensate for lack of capabilities each other, but there are some difficulties in noise rejection because the fre9uency range of ship noises covers the whole range vf biological sounds. The attempt to collect useful informations from underwater would be greatly contributed in fisheries.

• The Journal of the Korea Contents Association
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• v.21 no.1
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• pp.552-560
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• 2021

Currently, the domestic aquaculture industry is pursuing smartization, but it is still proceeding with human subjective judgment in many processes in the aquaculture stage. The prerequisite for the smart aquaculture industry is to effectively grasp the condition of fish in the farm. If real-time monitoring is possible by identifying the number of fish populations, size, pathways, and speed of movement, various forms of automation such as automatic feed supply and disease determination can be carried out. In this study, we proposed an algorithm to identify the state of fish in real time using underwater video data. The fish detection performance was compared and evaluated by applying the latest deep learning-based object detection models, and an algorithm was proposed to measure fish object identification, path tracking, and moving speed in continuous image frames in the video using the fish detection results. The proposed algorithm showed 92% object detection performance (based on F1-score), and it was confirmed that it effectively tracks a large number of fish objects in real time on the actual test video. It is expected that the algorithm proposed in this paper can be effectively used in various smart farming technologies such as automatic feed feeding and fish disease prediction in the future.

• Journal of fish pathology
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• v.9 no.2
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• pp.169-176
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• 1996

An indirect double antibody enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of a new virus isolated from abnormally swimming salmonid fish, RVS (Retrovirus of salmonid). Results using brain tissue homogenates, and infected cell cultures are described. The sensitivity of the methods is $10^{2.6}$ $TCID_{50}/100{\mu}l$ of the examined cell culture fluid. The specificity was confirmed by the ELISA inhibition test and virological examinations. Viral antigen could be detected in artificially infected fish tissue homogenates. The assay will allow the diagnosis of RVS-infected fish within a day.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.5 s.305
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• pp.79-84
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• 2005

In this paper, we developed an omni-directional surveillance and motion detection method. The fish-eye lens provides a wide field of view image. Using this image, the equi-distance model for the fish-eye lens is applied to get the perspective and panorama images. Generally, we must consider the trade-off between resolution and field of view of an image from a camera. To enhance the resolution of the result images, some kind of interpolation methods are applied. Also the moving edge method is used to detect moving objects for the object tracking.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.1
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• pp.83-88
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• 2010

Acoustic telemetry is a useful method to investigate fish behavior and is widely used to obtain biological information. In this study, the detection ability of a mooring-type acoustic telemetry system and the seasonal changes were studied for survey design and data analysis. The system detection range was examined with an underwater noise model, and seasonal changes were estimated with a ray-tracing program and underwater temperature profile data. The field experiment was conducted with two sets of pingers and six receivers to estimate the difference in detection rate by installation depth and to compare the model estimate. Results indicated that the long-range detection ability of the acoustic telemetry system was significantly affected by underwater temperature. The detection rate rapidly decreased near the sea surface or bottom despite that the near-range Signal to noise ratio was sufficient.

• Fisheries and Aquatic Sciences
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• v.8 no.3
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• pp.185-187
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• 2005

We tested the in vivo ability of a DNA chip to detect virus-specific genes from virus-infected olive flounder Paralichthys olivaceus and rainbow trout Oncorhynchus mykiss. Target cDNA was obtained from total RNA of virus infected cell lines by reverse transcription (RT) and was labeled with fluorescent dye (Cy5-dUTP). The results show the successful detection of infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV) genes in the virus-infected fishes.